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PubMed1967

Sphingomyelinase in normal human spleens and in spleens from subjects with Niemann-Pick disease.

This paper describes the purification and some of the properties of an enzyme from human spleen that catalyzes the hydrolysis of sphingomyelin with the formation of ceramide and phosphoryl choline. The enzyme, which is located in the subcellular particulate fraction that sediments between 700 and 8500 g, is readily made soluble and has been partially purified. Its pH optimum is between 4.5 and 5.0. It is unaffected by divalent cations, chelating agents, and sulfhydryl reagents, but is inhibited by phosphate. The enzyme attacks sphingomyelin and dihydrosphingomyelin, but is inactive toward sphingosine phosphoryl choline, O-acetylsphingomyelin, and lecithin. In some of its properties, the enzyme from human spleen is different from the previously studied sphingomyelinase from rat tissues. The enzyme is absent or markedly reduced in spleens from patients with classical and visceral varieties of Niemann-Pick disease, but is present in normal amounts in the late infantile type of the disease. In the present study another enzyme, this one magnesium-dependent, capable of catalyzing the cleavage of sphingomyelin has been detected in the spleens of patients with the classical form of Niemann-Pick disease. Some implications of these findings for theories of the metabolic defect in Niemann-Pick disease are discussed.

Adult↗

PubMed1982

Spleen cells from adult mice given total lymphoid irradiation (TLI) or from newborn mice have similar regulatory effects in the mixed leukocyte reaction (MLR). II. Generation of antigen-specific suppressor cells in the MLR after the addition of spleen cells from newborn mice.

Spleen cells from newborn BALB/c mice were added to the mixed leukocyte reaction (MLR) between a variety of responder and stimulator cells. The newborn cells nonspecifically suppressed the uptake of (3H)-thymidine and the generation of cytolytic cells regardless of the responder-stimulator combination used. Suppressor cell activity fell rapidly during the first 4 days after birth, and could not be detected by day 20. Newborn spleen cells inhibited the generation of nonspecific suppressor cells during the MLR but did not inhibit the generation of antigen-specific suppressor cells. Thus, newborn spleen cells exhibit a pattern of regulation of the MLR similar to that reported previously for spleen cells from adult mice given total lymphoid irradiation (TLI). These regulatory interactions provide a model that explains the ease of induction of transplantation tolerance in vivo in newborn mice and in TLI-treated adult mice.

Animals↗

PubMed1979

Cell killing by spleen necrosis virus is correlated with a transient accumulation of spleen necrosis virus DNA.

Spleen necrosis virus productively infects avian and rat cells. The average number of molecules of unintegrated and integrated viral DNA in cells at different times after infection was determined by hybridization and transfection assays. Shortly after infection, there was a transient accumulation of an average of about 150 to 200 molecules of unintegrated linear spleen necrosis virus DNA per chicken, turkey, or pheasant cell. No such accumulation was seen in infected rat cells. Soon after infection there was in chicken cells, but not inturkey, pheasant, or rat cells, also a transient integration of an average of 35 copies of viral DNA per cell. By 10 days after infection, the majority of this integrated viral DNA was lost from the population of infected chicken cells. At the same time, the majority of the unintegrated viral DNA was also lost from infected chicken, turkey, and pheasant cells. The transient cytopathic effect seen in these infected cells also occurred at this time. Late after infection about five copies of apparently nondefective spleen necrosis proviruses were stably integrated at multiple sites in chicken, turkey, pheasant, and rat DNA. These results demonstrate a correlation between the transient accumulation of large numbers of spleen necrosis virus DNA molecules and the transient occurrence of cytopathic effects.

Animals↗

PubMed2002

Anatomical rationale for spleen salvage by lobe/segment dearterialization in inferior pole spleen injury during left hemicolectomy: a post-mortem study.

The aim of this study was to determine the rationale for spleen salvage by lobe/segment dearterialization without resection for inferior pole injury during left hemicolectomy. One hundred and two consecutive human cadavers were dissected. Corrosion case and post-mortem arteriography with computerized planimetry were employed. Lobe/segment size, artery diameter and length and anastomoses between arteries were measured. The mean inferior terminal splenic artery had a significantly smaller diameter than the superior (2.8 vs. 3.4 mm, p<0.01). An inferior polar artery was found in 22.5% of the specimens (mean diameter, 1.9 mm; mean length, 33 mm). The inferior lobe and inferior polar segment comprised 41.3% and 12.6% of the spleen, respectively. Anastomoses were detected in 34 of 102 spleens (3% extraparenchymal, 88% intraparenchymal, 9% combined). The mean diameter and length of intrasplenic anastomoses were 0.3 mm and 20 mm, respectively. In conclusion, there was a positive correlation between diameters of lobar/segmental arteries and vascular zones ( p<0.05). The rationale for splenic lobe/segment dearterialization without resection is found in the presence of intrasplenic anastomoses.

Adult↗

PubMed1989

Human spleen cathepsin D: its characterization and localization in human spleen.

1. The cathepsin D was purified 1830-fold under mild conditions by a rapid procedure, based on two-step affinity chromatography. 2. Its molecular weight, amino acid composition and substrate specificity were shown to display minor differences from materials of other origins. 3. Inhibition with thiol compounds was found to be a specific phenomenon of the cathepsin D from the human spleen. 4. Production of antiserum specific for purified cathepsin D was demonstrated by immunodiffusion test, an immunoadsorbent column and immunoblotting of the crude enzyme in SDS gel. 5. In an immunocytochemical study, the antigenic sites for this enzyme were found to be localized in the reticuloendothelial system of the human spleen. 6. The role of this enzyme in human spleen cell was discussed.

Cathepsin D↗

PubMed1992

Response of pituitary and spleen pro-opiomelanocortin mRNA, and spleen and thymus interleukin-1 beta mRNA to adjuvant arthritis in the rat.

During development of adjuvant-induced arthritis (AA) in the rat, pituitary pro-opiomelanocortin (POMC) mRNA expression was increased. Pituitary POMC mRNA was much higher following adrenalectomy and AA. Spleen POMC mRNA also increased with a similar time kinetics, although the levels in the spleen were much lower than those in the pituitary. In control animals, spleen interleukin-1 beta (IL-1 beta mRNA) was undetectable, whereas AA led to the accumulation of IL-1 beta mRNA and the highest levels were seen in the adrenalectomised AA group. Thymic IL-1 beta expression was also increased in AA animals. These results suggest that AA leads to the activation of both the neuroendocrine and the immune systems and the interaction between these systems may play a role in this disease state.

Adrenalectomy↗

PubMed1996

Role of spleen or spleen products in the reduced locomotor-enhancing effect of morphine in diabetic mice.

In the present study, we examined the possibility that factor(s) derived from spleen cells are involved in the reduced morphine-induced hyperlocomotion in diabetic mice. The mean total locomotor activity after s.c. administration of morphine (20 mg/kg) in non-diabetic mice was significantly greater than that in diabetic mice. Splenectomized diabetic mice had a significantly higher sensitivity to morphine-induced hyperlocomotion than untreated or sham-operated diabetic mice. However, morphine-induced hyperlocomotion in non-diabetic mice was unaffected by splenectomy or sham-operation. Furthermore, 7 days after adoptive transfer of the supernatant of spleen cell homogenate (SSCH) from diabetic mice (SSCH-D), naive mice that had been injected with SSCH-D showed lower morphine-induced locomotor activity than mice which had been injected with SSCH from non-diabetic mice (SSCH-ND). These results suggest that some factor(s) derived from spleen cells may play an important direct or indirect role in the selective reduction of morphine's locomotor-enhancing effect in diabetic mice.

Animals↗

PubMed1989

The fuel of the spleen. Studies using a new method for perfusing the rat spleen with whole blood.

1. An improved rat spleen perfusion is described incorporating a method of defibrination which avoids the use of heparin and enables the spleen to be perfused with rat blood for several hours at a haematocrit of 40% and for 12 h or more at a haematocrit of 20%. 2. Glucose oxidation accounted for 11.6% of the total oxygen consumption but this represented only 8% of total glucose uptake, which was largely converted to lactate and released into the perfusate. However, significant amounts of lactate were oxidized. These results can be explained by the presence of at least two cell populations, one emphasizing the anaerobic oxidation of glucose and the other aerobic metabolism, particularly of lactate. 3. Non-esterified fatty acid and 3-hydroxybutyrate, when available at physiological concentrations, were shown to be major oxidative fuels of the spleen. 4. Chylomicron triacylglycerol was hydrolysed readily and taken up, but not oxidized extensively.

Animals↗

PubMed1984

[Sonographic determination of spleen size and spleen weight].

This report deals with the results of post-mortem sonographic examination of the spleen of 36 patients. The goal of the study was to gain direct information on the precision of the sonographic organometric method we use. It becomes evident that - depending on the position of the spleen in the dome of the diaphragm - the real length of the organ is underestimated when it is measured as the direct line between the two poles. For this reason the length should be taken as the (curved) median line obtained when performing a longitudinal section of the spleen at sonography. Under these conditions there is a good correlation of the sonographic and anatomic data obtained by autopsy. The mean deviation is maximally 7 mm. For the approximate calculation of the organ weight from the sonographically obtained data the simple ellipsoid formula is sufficient. In order to obtain a normogram for sonographic weight estimation a larger statistical sample is necessary.

Humans↗

PubMed1987

Splenic reticuloendothelial function after splenectomy, spleen repair, and spleen autotransplantation.

Overwhelming infection after splenectomy remains a problem despite the introduction of vaccine and antimicrobial prophylaxis. To evaluate prospectively various procedures proposed for salvage of the spleen, we measured reticuloendothelial function for two to five years in 51 patients who had initially presented with abdominal trauma and suspected splenic rupture. The mean percentage of pocked erythrocytes and the clearance of antibody-coated autologous erythrocytes in 8 patients who had splenic repair and in 6 who had partial splenectomy were the same as in 11 controls with intraabdominal injury that did not involve the spleen. The mean percentage of pocked erythrocytes remained significantly elevated in 19 patients who had undergone total splenectomy without autotransplantation of splenic tissue. One of seven patients who underwent splenic autotransplantation had a normal level of pocked erythrocytes 18 months after surgery, and a second patient had only a slight elevation at 24 months. The mean (+/- SEM) half-time clearance of labeled erythrocytes was significantly longer in the group that had total splenectomy without autotransplantation (421.1 +/- 74.5 hours) than in the autotransplantation group (91.6 +/- 20.0) or in the controls (5.4 +/- 2.0). We conclude that reticuloendothelial function was better preserved after partial splenectomy and splenic repair than after splenic autotransplantation, but that autotransplantation was superior to total splenectomy and appeared to be safe. Splenic autotransplantation deserves further study in patients who have had splenic trauma when other surgical maneuvers to save the spleen are not possible.

Adolescent↗

PubMed1976

The spleen in Friend leukemia. I. Prolonged survival of leukemic mice after autoimplantation of spleen tissue.

Friend virus infection of susceptible mice led rapidly to fulminant erythroleukemia and death. Subcutaneous implantation of leukemia spleen bits into splenectomized normal animals led to their early death from Friend leukemia. In contrast, bits of leukemic spleen implanted sc into splenectomized leukemic mice prolonged the survival of these animals. Concomitant with this survival was a reversal of the virus-induced immunosuppression and an increase in the levels of circulating, neutralizing, antivirus activity. This marked difference in response to leukemic spleen implants by leukemic as compared to normal mice reflected previous contact of the former with Friend Virus. Our studies indicated that the Friend virus-infected mouse mounted a resistance to the virus infection, which under certain conditions is capable of reversing the disease process.

Animals↗

PubMed1986

False-positive spleen imaging. Splenic cleft and accessory spleen.

Following trauma, a 35-year-old man had a falling hematocrit. Radiocolloid imaging showed a superior pole splenic defect with a bilobed appearance (suggesting nearly complete trans-section). There was a small quantity of function below the main portion of the spleen. At surgery, the spleen was noted to have marked lobulation (perhaps congenital) to account for the upper pole defect and trans-splenic appearance of a tear. A small accessory spleen was present below the main organ, mimicking the appearance of organ disruption. Cases of false-positive radiocolloid images, suggesting trauma, are rare but do occur.

Adult↗

PubMed2004

Spleen-preserving total pancreatectomy with conservation of the spleen vessels:: operative technique and possible indications.

Conventional distal pancreatectomy (cDP) and total pancreatectomy (cTP) also involve removal of the spleen. The spleen, however, is an important organ in the immunologic defense of the host and is worthy of preservation if this can be safely achieved. We performed a spleen-preserving total pancreatectomy (SPTP), with good results, in a Caucasian woman, 66-year-old, affected by pancreatic metastases of renal clear cell carcinoma. This is the first report of a SPTP for pancreatic metastases to our knowledge, and we therefore wish to describe the surgical technique and to suggest the possible indications for this new technique.

Adenocarcinoma, Clear Cell↗

PubMed1978

F1-hybrid anti-parental-strain reactivity. I. Impaired proliferation of CBA lymphocytes in the spleens of irradiated C3H X CBA mice pretreated with CBA spleen cells.

Previous investigations have indicated that C3H X CBA hybrid lymphocytes can neutralize lymphocytes which are reactive against the C3H-determined Mls antigen when injected into the H-2-compatible strain CBA. In this study we have inoculated C3H X CBA hybrids with CBA spleen cells and 2 weeks later lethally irradiated the mice and injected them with CBA lymphocytes to study their proliferation in the host spleen. Proliferation of lymphocytes from CBA mice, but not from C57Bl mice, was significantly impaired in such hosts, indicating that the hybrids had become immunized against the parental cells. In contrast, such an effect was not detected by treating CBA X C57Bl mice with CBA spleen cells. Humoral antibodies in the C3H X CBA hybrids capable of blocking the response of CBA lymphocytes against C3H X CBA cells could not be detected, indicating that inhibition of lymphocyte proliferation is cell mediated rather than by humoral factors.

Animals↗

PubMed1980

Proliferation of C3H x CBA hybrid lymphocytes in the spleens of irradiated CBA mice: inhibition of this phenomenon by pretreatment of the hosts with C3H x CBA spleen cells.

Lymphocytes from C3H x CBA hybrid mice proliferate intensely in the spleens of irradiated CBA mice. Pretreatment of the CBA hosts with C3H x CBA spleen cells, known to specifically reduce the T-cell reactivity of the hosts against the strongly stimulatory Mls-antigen determined by the C3H-genome, abolished the capacity of the host to promote proliferation of C3H x CBA lymphocytes. In contrast, proliferation of C3H x CBA bone marrow cells, as measured by splenic 59Fe incorporation, was unaffected by such pretreatment. By examining the capacity of spleen cell populations of (C3H x CBA) x CBA back-cross mice to inhibit lymphocyte proliferation, as described above, and to express the C3H-determined Mls-antigen, it was concluded that these two traits are associated.

Animals↗

PubMed1976

[Effect of spleen and bone marrow regeneration on the number of hematopoietic colonies in mouse spleen].

The spleen (2/3) was removed in CBA male mice (the 1st group); in the 2nd group the bone marrow from the right posterior shin was removed. The hemopoietic splenic colonies were counted on the 8th day after the lethal irradiation and injection of 1 X 10(-6) nucleated cells of the intact spleen. A significant increase of the number of colonies in comparison with their number in control intact mice was observed. The authors suppose that this increase could also be caused by the local influence of the regenerating stroma of the spleen and by some stimulating factor discharge by the regenerating hemopoietic tissue.

Animals↗

PubMed1975

Cellular cooperation during in vivo anti-hapten antibody responses. III. The helper cell activity of activated thymocytes, of spleen cells treated with anti-theta serum, and of spleen cells from anti-thymocyte serum-treated or adult thymectomized donors.

An adoptive secondary anti-2,4-dinitrophenyl (DNP) antibody response involving T-B cell collaboration has been studied. In particular, attempts have been made to affect the unexpectedly steep log dose-response curve obtained when graded numbers of helper cells are transferred to irradiated recipients given a fixed number of B cells (premium effect). A variety of means were used to alter helper cell activity, and this activity was then measured quantitatively, as was the ability of the helper cells present after these treatments to give a premium effect. It was shown that activated T cells are approximately twice as active as spleen cells in helper activity and give a comparable premium effect. Graded doses of anti-theta serum plus complement markedly reduce the helper activity of spleen cells without affecting the premium effect given by the residual cells. Treatment of primed cell donors with limited doses of heterologous anti-mouse thymocyte serum (ATS) before transfer does not affect B cell activity, but readily inactivates helper cells, again without affecting the premium effect given by the residual cells. Adult thymectomy (ATx) of helper cell donors before priming with carrier led initially to increased helper activity relative to age-matched control donors. This increase may reflect the loss of nonspecific suppressor T cells from spleens shortly after ATx. Late after ATx, there was about a 2-fold decrease in helper activity, probably reflecting a loss of helper cell precursors. At no time was there any change in the premium effect. In view of the failure of any of the techniques used to abolish the premium effect given by helper cells in this response, it seems likely that this premium effect is due to the cooperative interaction of two very similar types of mature T cell. Alternatively, the premium effect observed here may result from the interaction of two activities of a single type of T cell which is mediated by independent factors.

Animals↗

PubMed1975

Subpopulations of mouse spleen lymphocytes. II. Immunological reactivity of spleen cells fractionated on BSA density gradients.

We found in previous experiments that fractionation of non-immune mouse spleen cells on bovine serum albumin density gradients yields two subpopulations of T cells, one of high, the other of low density. Both subopopulations could be stimulated in corporate thymidine by the T cell-specific mitogen concanavalin A (con A). In the present investigation, spleen cells of mice immunized to sheep red cells (SRC) were similarly fractionated and the fractions recovered were assayed for: (a) reactivity to con A; (B) REACTIVITY TO SRC and (c) capacity to function as helper cells when stimulated with the homologous (SRC) or with a heterologous (donkey red cells) (DRC) antigen. Two subpopulations of cells reacting to con A were found in the spleens of the primed mice, corresponding to the subpopulations found in the non-immune mice. Both subpopulations contained cells responding to SRC (as measured by thymidine incorporation) and cells endowed with helper activity. The two subpopulations appeared to differ, however, in their specificity: while the denser cells could only exert their helper effect when stimulated by the specific antigen, the light cells could be effectively stimulated by both the specific (SRC) and the nonspecific (DRC) antigen.

Animals↗