Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Phlebotomus”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 91 records · Page 5Linked to original sources

Absence of outer caudal setae on all larval instars of Phlebotomus tobbi from the Ionian Greek islands.

Larval instars 2, 3 and 4 of Phlebotomus tobbi Adler, Theodor & Lourie from the Greek islands of Corfu and Zakynthos were found to have two caudal setae instead of the four usually present on these instars of Phlebotomus larvae. In a scanning electron microscope comparison with larvae of P. papatasi (Scopoli), a sensillum was seen in place of each outer seta of P. tobbi larvae, suggesting secondary loss of the setae. As the larvae of less than a tenth of the species and subspecies of the genus Phlebotomus have been seen and described, it cannot be assumed that those of P. tobbi are unique in having only two caudal setae. However, four caudal setae in late instars can no longer be considered as a constant character of the genus Phlebotomus. Observations on the larvae of P. tobbi also raise the question of the unknown function of the caudal setae.

Animals↗

A new morphological character to distinguish Sergentomyia and Phlebotomus.

Although the genera Sergentomyia, Phlebotomus and Lutzomyia are widely accepted, there is no character state which distinguishes them. Many Sergentomyia spp. have erect abdominal tergal setae normally characteristic of Phlebotomus; some Sergentomyia spp. lack cibarial armature "characteristic" of the genus, while some Phlebotomus spp. show this armature. Lutzomyia is "defined" by thoracic pleural setae and a cibarial bulge, both of which states occur on Old World forms. Lutzomyia may be defined geographically, but the use of geographical distribution in taxonomy leads to circular biogeographical arguments, and is invalid. The microtrichia of abdominal tergite six and sometimes four and five of the males of ten species of Sergentomyia (Subgenera Grassomyia, Sintonius, Sergentomyia and Parrotomyia) were found to be dimorphic. Those of 16 species of Phlebotomus, Chinius and Lutzomyia were monomorphic. In the subgenus: Parvidens, S. lesleyae males had dimorphic microtrichia on tergites five and six while those of S. heischi were monomorphic. Two other characters of tergal chaetotaxy, of probable taxonomic potential, are also described.

Abdomen↗

[Molecular systematics of Phlebotominae: a pilot study. Paraphyly of the genus Phlebotomus].

Phylogenetic relationships among Phlebotominae were inferred through a pilot study using parsimony analysis of the D2 domain of ribosomal DNA sequences: 455 pairs of bases were sequenced in nine species of Phlebotomine sandflies which belong to the genera Lutzomyia, Phlebotomus and Sergentomyia. Two taxa are used as outgroups: Psychoda sp. and Nemapalpus flavus which is the sister group of the Phlebotominae. The South American genus Lutzomyia appears to be monophyletic. The Mediterranean species Sergentomyia dentata is its sister group and is not clustered with the Old World genus Phlebotomus. The latter is a paraphyletic genus with an early individualization of the branch including the closely related subgenera Phlebotomus and Paraphlebotomus, and a late individualization of the subgenus Larroussius. These results have some consequences on the biogeography of the leishmaniasis in the Old World.

Animals↗

Species composition of sand flies and population dynamics of Phlebotomus papatasi (Diptera: Psychodidae) in the southern Jordan Valley, an endemic focus of cutaneous leishmaniasis.

The species composition of sand flies and the seasonality of Phlebotomus papatasi Scopoli were studied in the southern Jordan Valley from May to November 1992 using CDC light traps. Eleven species of sand flies were recorded, including P. kazeruni Theodor & Mesghali and P. tobbi Adler & Theodor, which are new records for the study area, and Sergentomyia squamipleuris Newstead, which is reported for the first time from Jordan. P. papatasi was the most abundant Phlebotomus species collected from domestic habitats as well as Psammomys obesus Cretzschmar burrows, comprising 89.4 and 99.5% of the total Phlebotomus catches, respectively. The catch of P. papatasi in CDC light traps was compared among domestic habitats, P. obesus burrows in an agriculturally modified semiarid rural habitat, and P. obesus burrows in a natural semiarid rural habitat. Peak P. papatasi abundance occurred in September and October and then declined sharply by late November. The abundance and temporal association of P. papatasi activity with the prevalence of cutaneous leishmaniasis in the study area pointed to the significance of this sand fly in the transmission of the parasite.

Animals↗

The roles of Phlebotomus martini and P.celiae (Diptera: Phlebotominae) as vectors of visceral leishmaniasis in the Aba Roba focus, southern Ethiopia.

During field studies (December 1988 to April 1990) to determine the vectors of visceral leishmaniasis (kala-azar) in the Aba Roba (Segen Valley) focus of southern Ethiopia, a total of 40,770 sandflies (Diptera: Psychodidae: Phlebotominae) were collected and identified as six species of Phlebotomus and seventeen Sergentomyia spp. Nine of these species were anthropophilic (four Phlebotomus and five Sergentomyia spp.), the dominant being Phlebotomus (Synphlebotomus) martini, P.(S.)celiae and Sergentomyia (Sergentomyia) schwetzi. P.celiae adult populations increased during the rainy season. Of 2326 P.martini and 1044 P.celiae dissected, the parous rate was 29.9% and 24.4%, respectively. Leishmania promastigotes were detected in sixteen P.martini (eleven identified as L.donovani by isoenzyme analysis and/or a DNA probe) and in three P.celiae (two identified by DNA probe), giving overall infection rates of 0.7% (1.9% parous infection rate) and 0.3% (1.2% parous infection rate), respectively. Four isolates from P.martini were typed by their isoenzyme profile as L.donovani zymodeme MON-37 = LON-44, identical to one of the zymodemes isolated from patients in this focus. This is the first evidence that P.celiae is a vector and the first time that P.martini has been implicated in Ethiopia. Based on observations of abundance, seasonality and prevalence of infection of the two Synphlebotomus spp., it is concluded that P.martini is the major vector and P.celiae a secondary vector in the Aba Roba focus. The risk of infection with L.donovani is greatest during the wet season.

Animals↗

Sand flies (Diptera: Phlebotominae) in Sanliurfa, Turkey: relationship of Phlebotomus sergenti with the epidemic of anthroponotic cutaneous leishmaniasis.

Sand fly (Diptera: Phlebotominae) fauna were surveyed in various districts of Sanliurfa in southeast Turkey for 3 yr immediately after an epidemic of cutaneous leishmaniasis (Leishmania tropica). Sticky papers and CDC light traps collected a total of 10,937 sand flies, of which 10,919 (4,158 females and 6,761 males) were identified as Phlebotomus and 18 (11 females and seven males) as Sergentomyia (S. theodori Parrot; S. adleri Theodor). Eight Phlebotomus spp. were identified: P. sergenti Parrot (72.3%), P. papatasi (Scopoli) (27.2%), P. brevis Theodor & Mesghali (0.20%), P. neglectus Leger & Pesson (0.13%), P. perfiliewi Parrot (0.05%), P. mascitti Grassi, P. halepensisTheodor, and P. alexandri Sinton (0.01%). Phlebotomus mascitti and P. neglectus, along with both Sergentomyia sp., have not been previously described from the study area. Similar results were obtained when both trapping methods were applied in the same houses, indicating that local P. sergenti and P. papatasi populations were equally attracted to the light. P. sergenti was consistently abundant, agreeing with the general view that this species is the vector of leishmaniasis in the region. There was no apparent decrease in the relative abundance of this vector versus the other species, suggesting that factor (s) other than a change in the dynamics of sand fly populations precipitated the decline of the human leishmaniasis epidemic in Sanliurfa.

Animals↗

Antigenic relationships among phlebotomus fever group arboviruses and their implication for the epidemiology of sandfly fever.

The antigenic relationships of 21 known or presumed Phlebotomus fever group serotypes and of 2 ungrouped, solvent sensitive, sandfly-associated arboviruses (Pacui and Charleville) were studied by complement fixation, plaque neutralization, and hemagglutination-inhibition methods. Results of complement fixation and neutralization tests were specific, allowing clear separation of the various serotypes, while those of the hemagglutination-inhibition test showed broader crossing and lack of specificity. Pacui virus was shown to be a member of the Phlebotomus fever serogroup. Six new Phlebotomus fever group serotypes are also described, increasing the known members of the group to 22. The implications of these and other recent data about the epidemiology of sandfly fever are discussed.

Animals↗

Enzyme polymorphism and genetic variability of laboratory populations of Phlebotomus papatasi, P. bergeroti, P. langeroni, and P. perniciosus.

Enzyme allomorph frequency and electrophoretic mobility were evaluated to clearly separate between morphologically similar sandfly species and to yield quantitative measures of their genetic similarity. Twelve enzyme systems were studied in laboratory colonies of Phlebotomus perniciosus, P. langeroni, P. papatasi, and P. bergeroti. Phlebotomus perniciosus and P. langeroni confirmed vectors of visceral leishmaniasis, were found to have fixed diagnostic allomorph differences at 3 enzyme loci (ICD-2, FUM, XDH). Phlebotomus papatasi, a known vector of cutaneous leishmaniasis and phleboviruses and P. bergeroti, a newly colonized suspected vector, were clearly differentiated from each other by a fixed allomorph at the MPI locus. Polymorphism at loci in these sandfly colonies ranged from 6.7% (P. perniciosus) to 43.7% (P. papatasi), with lowest levels seen in the older colonies. Expected heterozygosity was highest in P. papatasi, despite 33 generations of inbreeding, and comparable to that of P. bergeroti in its 7th generation. Against a scale of 0 for completely different, and 1.0 for identical genotypes, the enzyme data yielded indices of genetic identity (I) between P. perniciosus and P. langeroni and between P. papatasi and P. bergeroti of 0.783 and 0.737, respectively. These relatively high levels of genetic identity, paralleling similarities between species in morphology, inbreeding compatibility, and vectorial attributes, provide evidence of comparable and recent evolutionary divergence.

Animals↗

Vectorial efficacy of Phlebotomus argentipes in Kala-azar endemic foci of Bihar (India) under natural and artificial conditions.

Ability of Phlebotomus argentipes to acquire Leishmania donovani the causative agent of Indian Kala-azar was evaluated in the laboratory. Flies were fed artificially on infected blood suspensions, using a chick-skin-membrane feeding apparatus, and naturally on Leishmania donovani infected mice. In addition flies collected from different endemic areas were dissected and examined for natural infection. Flies fed on infected mice showed significantly higher feeding rate (14.4%, p < 0.01) compared to that of other experiments (9%, 8.75%) but the percentage of infection was very low (2.43%). No Chi-square comparison was made between infection rate and feeding rate because of low value in infection rate (less than 5). Flies dissected for natural infection showed only 0.1% infection. Not much difference was observed in the intensity of Leishmania donovani infection in the mid gut of sandflies examined from any of these experiments. These observations have confirmed that Phlebotomus argentipes has ability to acquire infection and it provides the final piece of evidence that Phlebotomus argentipes is the vector of Leishmania donovani in Bihar State.

Animals↗

[Natural foci of viruses borne by Phlebotomus papatasi in the USSR according to a serologic study of the population].

Seven hundred sixteen blood serum specimens from residents of presumable foci of phlebotomus fevers in Turkmenia, Tajikistan, Uzbekistan, Azerbaijan, and Moldavia were examined by the neutralization, complement fixation, hemagglutionation-inhibition and indirect immunofluorescence tests for the presence of antibody to viruses of the group of phlebotomus fevers (Sicilyan, Neapolitan, and Karimabad) and to rhabdovirus isfahan transmitted by phlebotomus papatasi. For the first time, antibody to Karimabad and Isfahan viruses were found in residents of the Central Asian republics. Antibody to Sicilyan and Neapolitan fevers were found in residents of all the republics examined. Data have been obtained indicating probable pathogenicity of Isfahan virus for man.

Antibodies, Viral↗

Distribution of Phlebotomus species in the Nile Delta, Egypt.

The importance of sandflies of the genus Phlebotomus being in the fact that some species are the vectors of leishmaniasis and other pathogenic organisms such as virus of sandfly fever. In Egypt, cutaneous leishmaniasis and sandfly fever are known and recently visceral leishmaniasis has been identified in Alexandria. Consequently, there is a need to study the geographical distribution of Phlebotomus species and to present an illustrative key for their identification. In the present study, four species of Phlebotomus were reported; P. papatasi, P. sergenti, P. langeroni and P. bergeroti. While P. papatasi is a known vector of zoonotic cutaneous leishmaniasis, P. langeroni is an incriminated vector for infantile visceral leishmaniasis, P. papatasi is the predominant species in the Nile Delta, but P. langeroni is found in West Alexandria and as a very rare species at the Libyan Egyptian borders. The whole results were discussed.

Animals↗

[Virus transmission to man from Phlebotomus flies: role of the Tuscany virus (Bunyaviridae, Phlebovirus) in the etiology of infections of the central nervous system].

During field studies on the ecology of arboviruses in Italy, strains of a new virus were isolated from sand flies, mostly females, of a pure colony of Phlebotomus perniciosus collected in a central Italian region (Tuscany). The prototype was named Toscana (TOS) virus, assigned to the Phlebotomus fever serogroup of arboviruses (family Bunyaviridae, genus Phlebovirus) and registered in 1980 in the International Catalogue of Arboviruses. Neutralizing antibodies to TOS virus were detected in human sera. Serologic surveys were carried out to estimate the natural distribution of TOS virus in Italy and it was shown that antibodies to this virus could be detected in humans mainly of the Tuscany region. In particular, a high infection rate (24.8%) was observed among residents of the province of Florence. Furthermore, several patients with a diagnosis of aseptic meningitis presented a serologic response which reflected a recent infection with TOS virus in the Tuscany and Marche regions. Consequently, between 1980 and 1986 studies were conducted at selected sites of the Tuscany region to determine the possible occurrence of vectors and presence of foci of TOS virus and to assess its public health importance. As a result of these investigations, several virus strains were isolated from pools of wild caught Ph. perniciosus and Ph. perfiliewi. Of the virus isolates obtained, 37 were identified as TOS virus and 47 as a new serotype, member of the Phlebotomus fever group. For this virus we suggested the name of Arbia (ARB) virus, Arbia being the river flowing across both Florence and Siena provinces from which the isolates originated.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Sandfly (Diptera, Psychodidae, Phlebotominae) fauna of South-Western Pakistan. 1. Diagnostic morphology of Phlebotomus papatasi (Scopoli), Ph. bergeroti (Parrot) and Ph. salehi (Mesghali).

A survey was conducted to study the morphology of the sandfly fauna in South-Western Pakistan (Balochistan). During the revision of different genera of sandflies the specimens of Phlebotomus papatasi (Scopoli) (N = 720), Ph. bergeroti Parrot (N = 30) and Ph. salehi Mesghali (N = 70) were encountered in various localities. These localities appear to be new records of the subgenus in the literature to date. Ph. bergeroti is reported for the first time from Pakistan and Ph. salehi from Balochistan as well. Characters of these three Pakistanese Phlebotomus are compared with the published data of these species from other countries. Keys for the identification of Pakistanese Phlebotomus are also constructed. Two female Ph. papatasi collected from indoors out of 132 female flies (1.5%) were found positive with flagellate infection in pharynx and midgut. The possible vectorial role of these flies is also discussed. Further surveys are necessary in parts of the country that have not been systematically surveyed.

Animals↗

Repellent efficacy of a combination containing imidacloprid and permethrin against sand flies (Phlebotomus papatasi) in dogs.

Infection in dogs and humans with the protozoan parasite Leishmania are widespread in tropical and subtropical countries around the globe. Sand flies of the order Phlebotomus in the Old World and Lutzomyia in the New World function as the vector of this disease. In dogs, skin lesions are the most prominent signs of canine leishmaniasis, besides other complex underlying manifestations. To prevent dogs from sand fly bites and thus transmission of Leishmania, an imidacloprid 10% w/v / permethrin 50% w/v combination was evaluated for its insecticidal and repellent efficacy. Treated and untreated control dogs were exposed weekly to about 200 female Phlebotomus papatasi for a period of four weeks. Dead and alive sand flies were counted for surviving rate evaluation and feeding rate was determined for repellency evaluation. The repellent efficacy was 94.6% (day 1), 93.3% (day 8), 80.0% (day 15), 72.8% (day 22) and 55.9% (day 29).The insecticidal efficacy was 60.0% (day 1), 46.2% (day 8), 42.6% (day 15), 35.2% (day 22) and 29.3% (day 29). The study demonstrated the high repellent potential of the imidacloprid / permethrin combination, thus protecting dogs from sand fly bites.

Animals↗

Leishmania major MON-26 isolated from naturally infected Phlebotomus papatasi (Diptera: Psychodidae) in Isfahan Province, Iran.

In a survey of leishmania infections in phlebotomine sandflies in one of the most important focus of zoonotic cutaneous leishmaniasis (ZCL) at Borkhar, a rural district north of the city of Isfahan, central Iran, Phlebotomus (phlebotomus) papatasi Scopoli from gerbil and jird burrows were naturally infected with Leishmania major zymodeme MON-26. This is the first isolation and characterization of L. major from P. papatasi in Iran, from an area where human and rodent infections with L. major have been present for a very long time.

Animals↗

Laboratory infection of the sandfly Phlebotomus papatasi Scopoli (Diptera, Psychodidae) with three Phleboviruses.

Membrane feeding and intrathoracic inoculation techniques were used to infect Phlebotomus papatasi sandflies with Phlebotomus fever group viruses. Sandflies were orally infected with Pacui virus, and were inoculated with Pacui, Sicilian sandfly fever and Naples sandfly fever viruses. Pacui virus did not multiply in these insects after oral infection, but an 8 X 10(4)-fold increase in virus concentration occurred within four days of intrathoracic inoculation of this virus. The infection rate was 100%. Sicilian sandfly fever virus multiplied in P. papatasi after infection by inoculation, although the yield was lower, a 20-fold increase in the mean virus concentration occurring over eight days. The results for Naples sandfly fever were inconsistent. A 32-fold increase in mean virus concentration was recorded in a small percentage of insects 14 days after inoculation of the virus.

Animals↗

Mapping the potential distribution of Phlebotomus martini and P. orientalis (Diptera: Psychodidae), vectors of kala-azar in East Africa by use of geographic information systems.

The distribution of two principal vectors of kala-azar in East Africa, Phlebotomus martini and Phlebotomus orientalis were analysed using geographic information system (GIS) based on (1) earth observing satellite sensor data: Normalized Difference Vegetation Index (NDVI) and midday Land Surface Temperature (LST) derived from advanced very high resolution radiometer (AVHRR) of the global land 1km project of United States Geological Survey (USGS), (2) agroclimatic data from the FAO Crop Production System Zone (CPSZ) of the Intergovernmental Authority on Development (IGAD) sub-region, and (3) the FAO 1998 soils digital map for the IGAD sub-region. The aim was to produce a predictive risk model for the two vectors. Data used for the analysis were based on presence and absence of the two species from previous survey collections in the region (mainly Ethiopia, Kenya and Somalia). Annual, wet season and dry season models were constructed. Although all models resulted in more than 85% positive predictive values for both species, the best fit for the distribution of P. martini was the dry season composite (NDVI 0.07-0.38 and LST 22-33 degrees C) with a predictive value of 93.8%, and the best fit for P. orientalis was the wet season composite (NDVI -0.01 to 0.34 and LST 23-34 degrees C) with a predictive value of 96.3%. The two seasonal composites models derived from satellite data were largely similar with best fit models developed based on the CPSZ climate data: average altitude (12-1900m), average annual mean temperature (15-30 degrees C), annual rainfall (274-1212mm), average annual potential evapotranspiration (1264-1938mm) and readily available soil moisture (62-113mm) for P. martini; and average altitude (200-2200m), annual rainfall (180-1050mm), annual mean temperature (16-36 degrees C) and readily available soil moisture (67-108mm) for P. orientalis. Logistic regression analysis indicated LST dry season composite of the satellite data, average altitude, mean annual temperature and readily available soil moisture of the CPSZ data as the best ecological determinants for P. martini while LST annual composite was the only important ecological determinant for P. orientalis. Spearman's rank correlation revealed several factors to be important determinants for the distribution of the two vectors. None of the soil types analysed appeared to be important determinant for the two species in East Africa, unlike in Sudan where P. orientalis is mainly associated with eutric vertisol (black cotton clay soil).

Africa, Eastern↗

Leishmania tropica: intraspecific polymorphisms in lipophosphoglycan correlate with transmission by different Phlebotomus species.

Lipophosphoglycan (LPG) is a dominant surface molecule of Leishmania promastigotes which has been shown to be critical for parasite-sand fly vector interactions. To provide additional evidence for its importance in transmission, the LPGs from three Leishmania tropica strains that differ in their capability to infect sand flies, were biochemically characterized. One of these strains, ISER/IL/98/LRC-L747, was isolated from a Phlebotomus sergenti female collected in the Judean Desert close to Jerusalem. The other strains originated from a different focus in the Galilee region of northern Israel. One was isolated from a patient (MHOM/IL/02/Ofri-LRC-L863) and the other from a naturally infected Phlebotomus arabicus female (IARA/IL/00/Amnunfly1-LRC-L810). The LPG structures of the isolates from the Galilee (L863 and L810) were similar to each other, but differed in the LPG repeat units from the Judean Desert isolate (L747). The terminal sugar in the side chains of the repeat units of LPG purified from L863 and L810 was beta-galactose and was not capped with glucose, unlike L747 and a previously characterized L. tropica strain from Iraq (L36). Since L810 was isolated from P. arabicus and L747 from P. sergenti, variations in the structure of their LPGs may explain their capacity to infect different sand fly species.

Animals↗