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At least 91 records · Page 5Linked to original sources
PubMed1991

Identification of a plant-derived mollicute as a strain of an avian pathogen, Mycoplasma iowae, and its implications for mollicute taxonomy.

Strain PPAV, a filamentous but nonhelical mollicute, was isolated from aborted apple seeds in France in late 1979. This organism grew well in SP-4 broth, fermented glucose, and required sterol for growth, and most of its properties suggested that it belonged to the genus Mycoplasma. However, it was serologically distinct; in addition, unlike other Mycoplasma species, genome measurements consistently yielded values of about 1,000 MDa (ca. 1,500 kbp), and the organism had a growth temperature optimum of 43 degrees C. A comparison of strain PPAV 16S rRNA sequences with those of other mollicutes revealed a high degree of sequence similarity to a strain of Mycoplasma iowae, which is commonly encountered in poultry. This relationship was confirmed by performing a restriction endonuclease pattern analysis and DNA-DNA hybridization tests. The genome size of type strain 695 of M. iowae was determined to be about 1,000 MDa (1,500 kbp) by renaturation kinetics, a value which is much higher than any other value known in the genus. Additional measurements by pulsed-field gel electrophoresis yielded values of 1,300 kbp for both strain PPAV and M. iowae. Subsequent phenotypic comparisons supported this relationship. Serologic tests with strain PPAV and other strains of M. iowae confirmed the findings of other investigators that this species is serologically heterogeneous. The high optimum temperature for growth of strain PPAV was also shared by a number of M. iowae isolates. Genome size is an inappropriate character for taxonomic assignment to the family Mycoplasmataceae because strain PPAV and other established species in this family are now known to have genomes ranging in size from 1,000 to 1,400 kbp.

Animals↗
PubMed1999

The effects of surface roughness and polymethylmethacrylate precoating on the radiographic and clinical results of the Iowa hip prosthesis. A study of patients less than fifty years old.

BACKGROUND: The purpose of the present study was to compare the results associated with two different surface finishes (bead-blasted and grit-blasted) for Iowa femoral components used in total hip arthroplasties performed between January 1979 and June 1991 in patients who were less than fifty years old. METHODS: Between January 1979 and December 1985, thirty-six primary total hip replacements were performed in twenty-five patients with insertion of a bead-blasted Iowa femoral component (average surface roughness, 0.8 micrometer) with cement and insertion of a titanium-backed acetabular component (thirty-five hips) or non-metal-backed acetabular component (one hip) with cement. Between January 1986 and June 1991, forty-five primary total hip replacements were performed in thirty-seven patients with use of a precoated grit-blasted Iowa femoral component (average surface roughness, 2.1 micrometers) and a Harris-Galante-I porous ingrowth acetabular component (forty-one hips) or an Osteonics component (four hips). The only change in the design of the femoral component (other than the surface finish) between the two consecutive series was the addition of polymethylmethacrylate precoating to the proximal third of all forty-five grit-blasted stems and modularity of the femoral head of the last eight grit-blasted stems. RESULTS: No hip was lost to follow-up. The duration of radiographic follow-up of the hips treated with the bead-blasted component averaged 11.3 years (range, ten to sixteen years) postoperatively, whereas that of the hips treated with the precoated grit-blasted component averaged 8.2 years (range, five to eleven years). Two (6 percent) of the bead-blasted femoral components were revised because of aseptic loosening compared with eight (18 percent) of the precoated grit-blasted components. Four bead-blasted components (11 percent) were either radiographically loose or were revised because of loosening compared with eleven precoated grit-blasted components (24 percent). Kaplan-Meier survivorship curves evaluated with log-rank analysis revealed that the bead-blasted Iowa femoral components were revised because of aseptic loosening (p = 0.0184) and were radiographically loose (p = 0.0068) less often than the precoated grit-blasted Iowa femoral components. CONCLUSIONS: The findings of the present study have led the senior ones of us to resume using femoral components with a polished surface and fixed with cement.

Adolescent↗
PubMed1990

Hb Iowa or alpha 2 beta 2(119)(GH2)Gly----Ala.

Hb Iowa with a Gly----Ala mutation at position beta 119(GH2) was observed in a Black infant and her mother. The baby was also heterozygous for Hb S; Hb Iowa was confused with Hb F at birth because its electrophoretic mobility was similar to that of Hb F1. The beta chain of Hb Iowa could be readily separated from the beta A, alpha, and gamma chains by polyacrylamide gel electrophoresis and by reversed phase high performance liquid chromatography. Structural characterization was through amino acid analyses of peptides isolated from a tryptic digest of the aminoethylated beta-Iowa chain. The Gly----Ala replacement in Hb Iowa does not affect its stability and oxygen carrying properties; hematological data for mother and child were within normal ranges.

Adult↗
PubMedDate not supplied

Amplification of Mycoplasma iowae using polymerase chain reaction.

Based on sequence data of Mycoplasma iowae recombinant DNA probe, pMI-12, two 25-base primers were synthesized for use in the polymerase chain reaction (PCR). An M. iowae species-specific 299-base pair product was amplified by the primers. An annealing temperature of 58.5 C was critical for detecting all members of this heterogeneous species while maintaining specificity of the M. iowae PCR. The minimum amount of target DNA detected by M. iowae PCR was 1 pg, which was 1000 times more sensitive than the dot-blot assay using M. iowae recombinant DNA probes.

Base Sequence↗
PubMed2005

The cost of containing one case of measles: the economic impact on the public health infrastructure--Iowa, 2004.

BACKGROUND: In February 2004, students from a college in Iowa, with a high proportion of nonmedical exemptions to vaccination, traveled to India; one fourth of the students contracted measles while there. One exposed, susceptible student returned home during his infectious period, necessitating 2 months of containment efforts in Iowa. OBJECTIVE: The objective of this study was to measure the direct costs of measles containment from a public health system perspective. METHODS: We evaluated activities performed, personnel time/materials allocated, and direct costs incurred in 2004 US dollars by the Iowa public health infrastructure. The study period was defined as March 5, 2004 (when the Iowa Department of Public Health was first contacted about the case), through May 12, 2004 (when a final meeting was held on the containment effort). RESULTS: A total of 2525 hours of personnel time were expended to review flight manifests, contact exposed passengers, set up vaccination clinics, trace >1000 potentially exposed contacts, and institute and enforce quarantine orders for vaccination refusers. Two thousand twenty-five phone calls were received from the public, and 2243 miles were driven by staff. The temporal distribution of personnel time was characterized by marked peaks at the report of potential secondary cases. The total estimated cost was 142452 dollars. CONCLUSIONS: The direct cost to the public health infrastructure of containing 1 case of measles was far greater than the estimated cost of uncomplicated individual illness (less than 100 dollars). Economic analyses of vaccine-preventable diseases may need to go beyond the costs of individual illness to account for the costs of protecting society.

Contact Tracing↗
PubMed1988

The incidence of pseudotumor cerebri. Population studies in Iowa and Louisiana.

We surveyed all neurologists, ophthalmologists, and neurosurgeons in Iowa, eastern Nebraska, and Louisiana over one year to determine the annual incidence of pseudotumor cerebri in residents of Iowa and Louisiana. The results were similar for both states. In Iowa, the annual incidence in the general population was 0.9 per 100,000 persons. When obesity was considered, this increased the incidence to 13/100,000 persons in Iowa and 14.85/100,000 persons in Louisiana for women 20 to 44 years of age who were 10% or more over ideal weight. Furthermore, the incidence became 19.3/100,000 for women in the same age range when they were 20% or more over ideal weight. The female-to-male ratio was 8:1, and the mean weight was 38% above ideal weight for height. Pseudotumor cerebri is a relatively common neurologic illness and may be an important preventable cause of blindness in obese young women.

Adult↗
PubMed2002

Abeta species, including IsoAsp23 Abeta, in Iowa-type familial cerebral amyloid angiopathy.

Mutations within the Abeta sequence of the AbetaPP gene are associated with familial forms of cerebral amyloid angiopathy (CAA). One mutation, Abeta D23N, was identified in a family in Iowa with a clinical history of early-onset dementia. We analyzed the pattern of Abeta deposition in the hippocampus of an individual with Iowa CAA. In addition to strong amyloid angiopathy, we found unusual diffuse Abeta deposits in the CA4, and in the parenchyma near amyloid-laden vessels. ELISA of cortical brain extracts showed that Abeta40 was nearly 20-fold higher than Abeta42, in both soluble and insoluble fractions. We identified an Abeta antibody that recognized wild-type Abeta but not Iowa Abeta. With this antibody, we found that wild-type Abeta was present in the Abeta deposits, but limited to the strongest deposits in the cerebrovasculature. Previous in vitro studies suggested that the presence of an asparagine at position 23 of Abeta favored formation of an isoAsp residue, which was associated with increased Abeta fibrillogenesis. Using isoAsp-specific antibodies in immunohistochemical studies, we examined the distribution of isoAsp Abeta in the Iowa brain. IsoAsp7 Abeta was present in both the parenchymal and vascular deposits, whereas isoAsp23 Abeta was present only in vascular deposits. These data suggest that alteration of Abeta Asn23 to isoAsp may be an important determinant in the deposition of Abeta in cerebral blood vessels.

Amyloid beta-Peptides↗
PubMed1991

The isolated bowel segment (Iowa model II) created in functioning bowel.

In experimental rats (n = 15), an isolated bowel segment (IBS) was created by (1) initial enteropexy between an 8-cm-long jejunal segment and the liver margin (hepatoenteropexy; Iowa model II) with its proximal and distal ends divided and immediately reanastomosed in an end-to-end fashion to reconstruct the bowel; and (2) secondary division of the IBS mesentery 5 weeks later. The IBS is then completely free of its mesentric and intramural nervous and vascular communications. The viability of the IBS is preserved by vascular collaterals developed at the hepatoenteropexy. Twelve rats proceeded to the second procedure, having tolerated regular rat chow with satisfactory weight gain. The Iowa model II created in functioning bowel was evaluated by contrast studies and myoelectrical activities. Contrast studies demonstrated peristalsis in the IBS. In the myoelectrical recordings, the frequency of slow wave was 32.5 +/- 1.0 in the IBS and 36.3 +/- 0.8 in the normal bowel (P less than .05). During fasting, the migrating motor complex (MMC) was observed to propagate aborally in the IBS in a coordinated fashion. The cyclic period of the MMC was 17.2 +/- 1.1 minutes in the IBS and 15.8 +/- 0.8 minutes in the normal bowel (P = .30). We conclude from this study that (1) the IBS (Iowa model II) retains motor function as demonstrated by successful feeding, as well as contrast studies and myoelectrical recordings that were essentially identical to those in the normal bowel; and (2) the IBS (Iowa model II) has significant research potential for studies of bowel physiology.

Anastomosis, Surgical↗
PubMed1995

The use of unofficial "problem patient" files and interinstitutional information transfer in emergency medicine in Iowa.

The study objectives were to determine the use of "problem patient" files in full-time Emergency Departments (EDs) in the state of Iowa and the frequency of telephone calls made between these departments about drug-seeking patients. The design and setting were a mail survey of 42 EDs in the state of Iowa determined to be staffed full-time by an in-house physician. The participants were the directors of the full time EDs in the state of Iowa. There were no interventions. The measurements and main results are as follows: 38 of 42 sites responded for a response rate of 90%. Of these, 58% keep problem patient lists and consult them an average of 2.6 times per week (range, 0.2 to 7.5). Only 5% have a policy limiting access to the information in these files, and only 14% have any controls over the adding of information. Thirty-seven of the 38 sites reported making and receiving calls to and from other institutions about drug-seeking patients. The mean number of calls made is 23 a year, and the mean number received is 20 per year. Problem patient files are common in EDs in Iowa, as are calls between institutions about drug-seeking patients. These practices compromise patient confidentiality but may be potentially justified in some instances. The lack of controls on access to patient information files and lack of scrutiny of information entered is of concern.

Communication↗
PubMed1994

Residential radon exposure and lung cancer: evidence of an urban factor in Iowa.

An ecological study of lung cancer, cigarette smoking, and radon exposure was conducted in 20 Iowa counties. County-based lung cancer incidence data for white female residents of Iowa were stratified according to radon level and smoking status. Cancer incidence data for the period 1973-1990 were obtained from the State Health Registry of Iowa. Smoking level was determined from a randomly mailed survey. Radon level was determined according to an EPA supported charcoal canister survey. Within low smoking counties, rates for all lung cancer and small cell carcinoma were significantly lower (p < 0.05) in the high radon counties relative to the medium and low radon counties. However, within high smoking counties, rates for all lung cancer, adenocarcinoma, and small cell carcinoma were significantly higher (p < 0.05) in the high radon counties relative to the low radon counties. Variations in socioeconomic data for these counties, available through the 1980 and 1990 census, did not explain these results. Lung cancer rates also were significantly increased in urban counties even after holding smoking status constant. Multivariate analyses revealed significant interactions between smoking, urbanization, radon levels, and lung cancer. The results of this hypothesis generating study will be tested in a case/control study now ongoing in Iowa. Analysis will need to include separate evaluations by smoking status, radon level, and residence in urban or rural areas for the major morphologic types of lung cancer.

Adenocarcinoma↗
PubMed1994

Factors that affect surgical rates in Iowa.

STUDY DESIGN: This study analyzed insurance claims to estimate the probability of medical and surgical treatments in different Iowa communities. The likelihood of surgical treatment was associated with patient characteristics of age and gender as well as hospital characteristics of size (number of beds), occupancy rate, and number of staff. OBJECTIVES: Our findings are being used by a study group of 25 physicians to understand the causes of variation in surgical rates for low back pain. Medical education and other interventions are being implemented. SUMMARY OF BACKGROUND DATA: Hospitalization rates for lower back operations in the United States increased by more than 20% from 1978 to 1985. Consequently, several studies in Iowa and the US have been initiated to examine the medical effectiveness of these treatments. METHODS: A logistic regression model was used to determine the factors associated with the likelihood of having a low back surgery in a population of Blue Cross/Blue Shield (BCBS) subscribers in Iowa. The outcome, or dependent variable, of interest was a hospitalization that resulted in a surgical procedure on a low back pain patient. RESULTS: Surgical rates for the treatment of low back pain are likely to be increased if a BCBS Iowa subscriber is female, older than 44 years of age, or if the surgery is performed in a hospital with either an occupancy rate less than 62%, with fewer than 774 staff members, fewer than 267 beds, or no residency programs.

Adult↗
PubMed1990

Effects of the elimination of a state monopoly on distilled spirits' retail sales: a time-series analysis of Iowa.

Since the end of prohibition in the USA, only one state, Iowa, has eliminated its state retail monopoly of distilled spirits sales for off-premise consumption. In March 1987 all state retail stores were closed, and private establishments were licensed to sell distilled spirits. Interrupted time-series analyses of apparent spirits consumption in Iowa, controlling for nationwide sales trends over the past two decades, identified a statistically significant 9.5% increase in spirits sales following the policy change. While there was a corresponding 13.7% decline in wine sales, and no change in beer sales, privatization of spirits retail sales yielded a net increase in total alcohol consumption in Iowa. No changes were found in spirits sales in states bordering Iowa.

Alcohol Drinking↗
PubMedDate not supplied

Iowa PK Press for donor corneas--a comparative study of donor corneal shape.

BACKGROUND: A new cornea press for cutting donor corneal buttons from the endothelial side, the Iowa PK Press, is described. Major modifications of the Bourne Press include a spring-activated piston without lateral sway, a centering device for a Teflon cutting pad, and the ability to accommodate a wider range of trephine sizes (6.2 mm to 10.0 mm). METHODS: The Iowa PK Press was compared with the Bourne Corneal Press and trephination by hand in a series of 15 donor cornea trephinations. Measurements taken from photographs of the buttons included endothelial diameter, epithelial diameter, and edge angles. RESULTS: The Iowa PK press cut buttons that had a slightly larger epithelial and endothelial diameter when compared to hand trephination (p < .05, analysis of variance) with less undercutting of the endothelial surface. There was no statistically significant difference in epithelial and endothelial diameters, eccentricity, or edge angles between the two presses. CONCLUSIONS: The Iowa PK Press is more versatile than the Bourne Press in terms of the potential range of diameter of corneal buttons that can be created.

Cell Size↗
PubMed1990

Relationship between diabetes mellitus mortality rates and drinking water magnesium levels in Iowa.

A recent ecologic study has suggested a relationship between mortality from diabetes mellitus and levels of magnesium in drinking water. However, diabetes mortality rates were from aggregate data for the entire geographic area under study. The state of Iowa has data resources available to determine diabetes mortality rates for individuals on known water supplies in Iowa and magnesium levels for raw drinking water supplies. To determine the presence of this relationship in the state of Iowa, mortality rates from 1976 through 1985 for diabetes as an underlying cause of death and for major cardiovascular disease with diabetes as an ancillary cause of death were compared for individuals from towns in Iowa with populations of 1,000 to 10,000. At the stratification levels of magnesium in municipal drinking water used in this study, no significant differences were found in diabetes mortality rates.

Cardiovascular Diseases↗
PubMed1982

Ischemic heart disease mortality in Iowa farmers. The influence of life-style.

Analysis of 62,000 deaths in Iowa men aged 20 to 64 years from 1964 to 1978 indicates that Iowa farm men younger than 65 years have lower than expected mortality for all causes and for ischemic heart disease. Using data gathered by the Iowa Lipid Research Clinics (LRC) Program Prevalence Study, we compared levels of known cardiovascular risk factors in farmers with those of nonfarmers residing in the same rural county. Farm men smoked less frequently (19% v 44%) and engaged in exercise more frequently (83% v 40%) than nonfarmers. Treadmill performance in farm men indicates a higher level of fitness in farmers. Farm people consumed more total calories and cholesterol, but drank less alcohol than nonfarmers. Farm men had significantly higher serum cholesterol levels. High-density lipoprotein cholesterol levels were also higher in farmers. However, when adjusted for age and exercise status, farmers and nonfarmers had similar lipid levels. The results of the LRC survey suggest that the lower mortality rates of Iowa farmers are a reflection of a life-style that includes vigorous exercise and little consumption of alcohol and tobacco.

Adult↗
PubMed1996

Specific detection of Mycoplasma iowae using polymerase chain reaction.

A polymerase chain reaction assay was developed for the specific detection of Mycoplasma iowae, a poultry pathogen. Two primers were chosen in the DNA region located immediately 5' of the ribosomal genes operon. Under our PCR conditions, specific amplification of all M. iowae strains tested was achieved, and none of 17 other Mycoplasma species isolated from birds gave an amplification product. We obtained amplification from culture medium samples of M. iowae by using Tth DNA polymerase instead of Taq DNA polymerase, and we were able to detect as few as 10 organisms. Finally, we described a dot blot hybridization test, using cold labelling and chemiluminescence, which is very convenient for routine detection of M. iowae.

Animals↗
PubMed2004

Emotion-based learning on a simplified card game: the Iowa and Bangor Gambling Tasks.

The Iowa Gambling Task (IGT) has been widely used in the assessment of neurological patients with frontal lesions. Emphasis has been placed on the complexity of the task (i.e., four decks of varying contingency pattern) with the suggestion that the participant must use emotion-based learning to deal with a complex decision-making process. The present study used a single deck card game (the Bangor Gambling Task, BGT), matched in many respects with the Iowa Gambling Task, in which the contingencies varied over time (gradually becoming worse for the participant) rather than across deck (as in the IGT). Forty participants performed both tasks. Performance on the tasks showed many similarities, with participants showing a comparable pattern of incremental learning on both tasks, reaching an analogous final level of performance. More importantly, there was a high correlation (r(2) = .93) in performance between the two tasks, the most salient feature of which was that virtually every participant who fell below categorisation of impaired IGT performance, also did very poorly on the BGT. These findings bear on the question of whether arguments about the 'complexity' of the Iowa Gambling Task necessarily explain why it appears to require emotion-based learning. The Bangor Gambling Task might also be a useful tool for clinical neuropsychologists, in the assessment of patients with executive dysfunction-given that the task is easier and quicker to administer than the Iowa Gambling Task, but appears to share the same performance features.

Adult↗
PubMed1998

Deregulation and Cross-Border Substitution in Iowa's Riverboat Gambling Industry.

When riverboat casinos began operating in Iowa in April 1991 a strict regulatory structure, consisting of mandated sailing, wagering and loss limits, and casino space restrictions, was imposed. The restrictions were removed in May 1994 after Illinois introduced riverboat gambling without space, betting, or loss limits. Using data on casino win and admissions in Iowa and Illinois, this study analyzes the impact that deregulation had on casino win, win per admission, and total admissions. The findings reveal that deregulation significantly increased casino win, win per admission, and total admissions in Iowa. Moreover, when focusing on the "Quad Cities" market, which encompasses both Iowa and Illinois casinos, deregulation is found to result in significant cross-border substitution. In addition, there is evidence that deregulation has partly resulted in existing gamblers losing more money as opposed to increasing the number of gamblers.

Journal Article↗