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PubMed2003

Purification and properties of an ice-nucleating protein from an ice-nucleating bacterium, Pantoea ananatis KUIN-3.

An ice-nucleating protein (INP) from the extracellular ice-nucleating matter (EIM) of Pantoea ananatis (Erwinia uredovora) KUIN-3 was purified and characterized. The EIM produced by the strain KUIN-3 was purified by ultrafiltration, sucrose density-gradient ultracentrifugation and gel filtration. The INP was purified using of column chromatography on hydroxyapatite and Superdex 200 in the nondenaturing detergent of 0.1% (w/v) Triton X-100. The purified INP was composed of one subunit of 117 kDa according to SDS-PAGE. It has become apparent that the INP was the ice-nucleating lipoglycoprotein based on the reaction of carbohydrate stain and lipid stain with the INP. It was inhibited by p-mercuribenzoate and N-bromosuccinimide. The activity of the INP gradually decreased from 65 degrees C. The pH stability was held between pH 7.0 and pH 11.0. The INP had a lower ice-nucleating temperature below pH 6.0. It has become apparent that the INP consisted of the class C structure in the EIM based on its freezing difference spectrum in D2O versus H2O.

Journal Article↗
PubMed2006

Fifth-order two-dimensional Raman spectroscopy of liquid water, crystalline ice Ih and amorphous ices: sensitivity to anharmonic dynamics and local hydrogen bond network structure.

The theoretical study of off-resonant fifth-order two-dimensional (2D)-Raman spectroscopy is made to analyze the intermolecular dynamics of liquid and solid water. The 2D-Raman spectroscopy is susceptible to the nonlinear anharmonic dynamics and local hydrogen bond structure in water. It is found that the distinct 2D-Raman response appears as the negative signal near the t(2) axis. The origin of this negative signal for t(2)<15 fs is from the nonlinear polarizability in the librational motions, whereas that for 30 fs<t(2)<150 fs is attributed to the anharmonic translational motions. It is found that the mechanical anharmonicity and nonlinear polarizability couplings among modes clearly can be observed as the sum- and difference-frequency peaks in the 2D-Raman spectrum (i.e., Fourier transforms of the response). The 2D-Raman spectroscopies of ice Ih and amorphous ices, i.e., low density, high density, and very high density amorphous ices, are also investigated. It is found that the 2D-Raman spectroscopy is very sensitive to the anisotropy of the structure of ice Ih. The strong hydrogen bond stretching band is seen in the 2D-Raman spectroscopy of the polarization directions parallel to the c axis, whereas the contributions of the librational motion can be also seen in the spectrum with the polarization directions parallel to the a axis. The 2D-Raman spectroscopy is also found to be also very sensitive to the differences in local hydrogen bond network structures in various amorphous phases.

Journal Article↗
PubMed1997

Human vascular smooth muscle cells express interleukin-1beta-converting enzyme (ICE), but inhibit processing of the interleukin-1beta precursor by ICE.

Local immunoregulatory processes during normal vascular biology or pathogenesis are mediated in part by the production of and response to cytokines by vessel wall cells. Among these cytokines interleukin (IL)-1 is considered to be of major importance. Although vascular smooth muscle (SMC) and endothelial cells (EC) expressed both IL-1alpha and IL-1beta as cell-associated, 33-kilodalton (kD) precursors, SMC neither contained detectable mature IL-1beta, nor processed recombinant IL-1beta precursor into its mature 17-kD form. Thus, we investigated the expression and function of IL-1beta-converting enzyme (ICE) in vascular cells. We demonstrate in processing experiments with recombinant IL-1 precursor molecules that EC processed IL-1beta, in contrast to SMC. Despite the failure of SMC to process IL-1beta, these cells expressed ICE mRNA, immunoreactive ICE protein, and the expected IL-1beta nucleotide sequence. The lack of processing was explained by our finding that extracts of SMC specifically and concentration dependently blocked processing of IL-1beta precursor by recombinant or native ICE. The initial biochemical characterization of the inhibitory activity showed that it is heat-labile, has a molecular size of 50-100 kD, and is associated to the cell membrane compartment. Inhibition of processing, i.e., activation of IL-1beta precursor by SMC may constitute a novel regulatory mechanism during normal vascular biology or pathogenesis of vascular diseases.

Caspase 1↗
PubMed2001

On-ice performance characteristics of elite and non-elite women's ice hockey players.

The purpose of this study was to compare skating performance and on-ice fitness of elite and non-elite women's ice hockey players. Twenty-three players who were participants in an organized women's hockey training program were tested. Seven of the elite players were members of the 1996/ 1997 Canadian National Team and 1 was from the Finnish National Team. Skating tests included (a) agility cornering S turn (AGL), (b) 6.10-m acceleration (ACC), (c) 47.85-m speed (SPD), (d) 15.20-m full speed (FSPD), and (e) Reed repeat sprint skate test (RRS). Elite players were older (25 +/- 5.34 vs. 19 +/- 2.65 years) and had better performance in the following variables: FSPD (1.88 +/- 0.11 vs. 1.96 +/- 0.86 seconds); RRS, drop-off percent (23.25 +/- 5.85% vs. 34.98 +/- 8.45%), drop-off time (3.43 +/- 0.78 vs. 5.35 +/- 1.23 seconds); sum of 6 repeats (101.96 +/- 2.74 vs. 112.78 +/- 4.61 seconds); and anaerobic capacity (AnCap; 5.35 +/- 0.14 W x kg(-1) vs. 4.87 +/- 0.19 W x kg(-1)) as calculated by the formulas of Watson and Sargeant (23). These results suggest that elite women's ice hockey players are older, faster skaters; have a better drop-off percent, drop-off time, and sum of 6 repeats in a repeat skate test; and have greater on-ice anaerobic capacity than non-elite players.

Adolescent↗
PubMed1989

An ice nucleation active gene of Erwinia ananas. Sequence similarity to those of Pseudomonas species and regions required for ice nucleation activity.

The ice nucleation active gene, inaA, of Erwinia ananas IN-10 has been sequenced. This gene encodes a protein composed of 1322 amino acid residues. The inaA protein contains a 1120-residue segment consisting of 70 repeats of closely related 16 amino acid motifs (R-domain), which is flanked by N- and C-terminal sequences (N- and C-domains, respectively). Its primary structure is similar to, but not identical with, those of Pseudomonas inaW and inaZ gene products. By truncating the inaA gene to various extents, it was found that deletion of the C-domain resulted in complete loss of the ice nucleation activity, whereas removal of the N-domain led to a moderate decrease in the activity. Complete loss of the activity was also observed when the N-domain plus a large part of the P-domain were deleted. It is suggested that the C-domain is required for the assembly of inaA protein to form a functional ice nucleus.

Amino Acid Sequence↗
PubMed1998

Sanitary care and maintenance of ice-storage chests and ice-making machines in health care facilities.

In response to a reported hospital outbreak traced to the use of contaminated ice in 1968, the Centers for Disease Control and Prevention (CDC) developed an advisory regarding the sanitary care and maintenance of ice-storage chests and ice-making machines. CDC has revised this unpublished advisory several times during the years to respond to requests for guidance from infection control professionals. Because CDC continues to receive inquiries about this topic from infection control professionals, this advisory is being published.

Centers for Disease Control and Prevention, U.S.↗
PubMed2002

The impact of wood ice cream sticks' origin on the aroma of exposed ice cream mixes.

The effect of volatile compounds in white birch sticks obtained from four different geographical locations on the aroma of ice cream mix was investigated. Sensory evaluation, (specifically, a series of warmed-up paired comparisons) was conducted on stick-exposed ice cream mixes to determine whether aroma differences in those mixes could be detected. Batches of ice cream mix were exposed to the sticks and aged for 6 d at 4 degrees C and then assessed by the panelists by pairwise comparison. Findings suggest that differences in aroma of mixes that have been exposed to white birch sticks from four different geographical origins can be distinguished perceptually.

Food Technology↗
PubMed1998

Modified ice study: a phase II study of an intensive, modified ICE regimen (ifosfamide, carboplatin and etoposide) in patients with better prognosis, small cell lung cancer.

A total of 30 with good prognosis small cell lung cancer were treated with a modified 'ICE' (ifosfamide, carboplatin and etoposide) chemotherapy regimen in an attempt to achieve a high response rate with less toxicity than is seen with the full 'ICE' regimen. This was given every 4 weeks for a maximum of six cycles. In total 25 patients (83%, 95% CI (70-97%)) experienced a partial or complete response at some stage of their treatment. Of these patients, 12 (40%, 95% CI (22-58%)) showed a complete response. A total of 19 patients (63%) had to have their dose reduced and/or delayed at some point due to toxicity. Nadir blood counts showed that 19 patients (63%, 95% CI (46-81%)) had WHO grade 3 or 4 thrombocytopenia, and 24 (86%, 95% CI (73-99%)) had grade 3 or 4 neutropenia. A total of 17 patients (53%) completed six cycles of chemotherapy. In total 3 patients died during treatment all due to treatment-related complications. Median survival was 12.6 months (95% CI (11.6, 14.7 months)). Nausea, vomiting, dysphagia, activity, mood and overall condition, as recorded using daily diary cards, were worse at the beginning of each chemotherapy cycle. Both response rates and survival were clinically acceptable. However, neutropenia and thrombocytopenia, although reduced from rates reported with the full ICE regimen, were still high. A prospective randomised controlled trial is now needed to assess this regimen in more detail.

Adult↗
PubMed2005

Chemistry of ice surfaces. Elementary reaction steps on ice studied by reactive ion scattering.

This Account describes a recent study of reactions on ice surfaces with the emphasis on the mechanistic features of elementary reactions steps. Cs(+) reactive ion scattering (Cs(+) RIS) and low-energy sputtering (LES) techniques monitor the reactions by detecting the molecules and ions on the ice surface. The types of reactions include molecule diffusion and migration, proton transfer, and some simple reactions on frozen water and alcohol surfaces. Ice surface reactions exhibit unique behaviors due to a kinetic constraint, resulting in the isolation of reaction intermediates, preferential stabilization of charged species, and diversity of reaction products.

Journal Article↗
PubMed1996

Identification and characterization of Ich-3, a member of the interleukin-1beta converting enzyme (ICE)/Ced-3 family and an upstream regulator of ICE.

We report here the isolation and characterization of a new member of the ice/ced-3 family of cell death genes, named ich-3. The predicted amino acid sequence of Ich-3 protein shares 54% identity with murine interleukin-1beta converting enzyme (ICE). Overexpression of ich-3 in Rat-1 and HeLa cells induces apoptosis, which can be inhibited by CrmA and Bcl-2. The mRNA and proteins of ich-3 are dramatically induced in vivo upon stimulation with lipopolysaccharide, an inducer of septic shock. The ich-3 gene product can be cleaved by cytotoxic T cells granule serine protease granzyme B, suggesting that Ich-3 may mediate apoptosis induced by granzyme B. Ich-3 does not process proIL-1beta directly but does promote proIL-1beta processing by ICE. These results suggest that Ich-3 may play a very important role in apoptosis and inflammatory responses and may be an upstream regulator of ICE.

Amino Acid Sequence↗
PubMed2004

Pulmonary function decay in women ice hockey players: is there a relationship to ice rink air quality?

Fossil-fueled ice rink resurfacing machines emit high levels of ultrafine and fine particulate matter (PM(1)) and may be related to asthmalike symptoms in skaters. We examined PM(1) exposure and airway status in elite women ice hockey players over 4 training years. Lung function, asthma symptoms, and rink PM(1) were evaluated. Pre- and postexercise spirometry was performed on 14 female hockey players and 9 female control nordic skiers 4 times over 4 yr. Baseline lung functions were normalized to height cubed (Ht(3)) and recalculated to subject mean height (1.69 m) to evaluate change. Venue CO, NO(2), and PM(1) were measured. Training history for hockey players included 2 yr in a low-[PM(1)] rink, followed by transition to high-[PM(1)] fossil fuel machine resurfaced rinks; [PM(1)] for control ski venue was low. [CO] and [NO(2)] were acceptable at all venues. Controls showed no baseline function change over 4 yr. For hockey players, 1997 lung function values at the low-[PM(1)] venue were significantly higher than 2001 high-[PM(1)] venue values (p <.05); decay per year between 1997 and 2001 was greater for FEF(25-75) (251 +/- 185, 83 +/- 40, 109 +/- 58, 109 +/- 187 ml yr(-1), mean +/- SD for FEF(25-75), FVC, FEV1, PEF, respectively; p <.05). No relationships between baseline lung functions and airway hyperresponsiveness or symptoms were identified. Five of 9 controls had symptoms, and 10 of 14 subjects had symptoms. This preliminary study suggests [PM(1)] is related to airway function decay in ice rink athletes.

Administration, Inhalation↗
PubMed1987

Ice Encasement Injury to Microsomal Membranes from Winter Wheat Crowns : I. Comparison of Membrane Properties after Lethal Ice Encasement and during a Post-Thaw Period.

The functional and physical properties of cellular membranes isolated from Triticum aestivum, cvs Norstar and Fredrick, were altered coincident with changes in composition after a lethal ice-encasement stress and further during a 6 hour post-thaw period. Crowns encased in ice for a duration which inhibited regrowth, exhibited enhanced rates of electrolyte leakage. Furthermore, the recovery of total microsomal protein and phospholipid declined, suggesting that some membrane degradation had been induced during the anoxic stress. The microviscosity of microsomes and liposomes prepared from such membranes increased during stress, and this was correlated with a 2- to 4-fold increase in the free fatty acid levels in the microsomal fraction. There was, however, only a relatively minor change in fatty acid unsaturation during the ice-encasement stress. The process continued during a 6 hour aerobic post-thaw treatment, but the pattern was somewhat different. During this phase, the leakage of electrolytes was further increased and the recovery of microsomal protein and phospholipid continued to decline, indicating general degradation; but, in contrast to the anoxic phase, the degree of fatty acid unsaturation declined markedly, indicating lipid peroxidation.

Journal Article↗
PubMed2003

Ice core evidence for Antarctic sea ice decline since the 1950s.

The instrumental record of Antarctic sea ice in recent decades does not reveal a clear signature of warming despite observational evidence from coastal Antarctica. Here we report a significant correlation (P < 0.002) between methanesulphonic acid (MSA) concentrations from a Law Dome ice core and 22 years of satellite-derived sea ice extent (SIE) for the 80 degrees E to 140 degrees E sector. Applying this instrumental calibration to longer term MSA data (1841 to 1995 A.D.) suggests that there has been a 20% decline in SIE since about 1950. The decline is not uniform, showing large cyclical variations, with periods of about 11 years, that confuse trend detection over the relatively short satellite era.

Journal Article↗
PubMed1999

Peptide based interleukin-1 beta converting enzyme (ICE) inhibitors: synthesis, structure activity relationships and crystallographic study of the ICE-inhibitor complex.

Based on the X-ray structure of the complex of Ac-Tyr-Val-Ala-Asp-H (L-709049) and interleukin-1 beta converting enzyme (ICE), we synthesized compounds which were derived from 2-NapCO-Val-Pro-Asp-CH2OPh (1) to obtain a potent inhibitor in the cell assay. Among these compounds, (3S)-N-methanesulfonyl-3-[[1-[N-(2-naphthoyl)-L-valyl]-L-prolyl]amino]- 4-oxobutanamide (27c) showed high potency not only in the enzyme assay but also cell assay with IC50 values of 38 nM and 0.23 microM, respectively. Compound 27c, with a c log P value of 1.76, had a more hydrophilic character compared with 1. Compound 27c also dose dependently inhibited LPS-primed ATP-induced IL-1 beta release in mice. The crystal structure of the complex of compound 27c and ICE revealed that compound 27c had further interactions with ICE in the naphthoyl group at the P4 position and in the methyl group of the methanesulfonamidecarbonyl group at the P1 position, compared with L-709049. To our knowledge, compound 27c is the first example that shows a strong inhibitory activity without the carboxyl group at the P1 position.

Animals↗
PubMed1986

Laboratory and on-ice test comparisons of anaerobic power of ice hockey players.

The suitability of the Wingate Anaerobic Test (WAT40) as a laboratory measure of anaerobic capacity (AnCap) and power (AnPow) of ice hockey players was tested against the Reed Repeat Sprint Skate-RSS (1979) and the Sargeant Anaerobic Skate (SAS40). Twenty-four university and Junior A players (20.2 +/- 1.6 years), assigned by random draw, performed the three tests over a seven day period. Blood lactate taken from an unwarmed finger tip was used to assess work intensity. The AnCap (7.7 +/- 0.2 Watts X kg-1) and AnPow (10.1 +/- 0.2 Watts X kg-1) for WAT40 were significantly lower (p less than 0.05) than for RSS (AnCap 9.3 +/- 0.8 Watts X kg-1; AnPow 11.5 +/- 1.1 Watts X kg-1) and SAS40 (AnCap 9.7 +/- 0.8 Watts X kg-1; AnPow 11.9 +/- 1.8 Watts X kg-1). SAS40 was significantly higher (p less than 0.05) than RSS for both AnCap and AnPow. The RSS (r = 0.96; ME 4.5%) and SAS40 (r = 0.97; ME = 3.6%) showed excellent test-retest reliability and reproducibility for AnCap but were only fair on AnPow (RSS: r = 0.73; ME = 10.7%; SAS40: r = 0.65; ME = 18.4%). While the correlations among the tests (AnCap: SAS40 vs WAT40, r = 0.73; RSS vs WAT40, r = 0.69) were significant (p less than 0.05), the highest predictive capability estimate (r2) was only 53.3%. The correlations for blood lactates (WAT40: 10.8 +/- 1.5 mmol X l-1; SAS40: 10.7 +/- 1.9 mmol X l-1; RSS: 11.5 +/- 1.6 mmol X l-1) were not significant. Based upon the particular protocol used, the laboratory test WAT40 does not demonstrate a high relationship with on-ice measures of AnCap and AnPow in this group of ice hockey players.

Adolescent↗
PubMed1987

Evaluation of the effectiveness of wet ice, dry ice, and cryogenic packs in reducing skin temperature.

The purposes of this study were to evaluate and compare the ability of wet ice (WI), dry ice (DI), and cryogenic packs (CGPs) to reduce and maintain the reduction of skin temperature directly under the cooling agent and to determine whether the cooling effect on skin extended beyond the surface area in contact with the cooling agent. Ten female volunteers participated in the study, and each of the three cold modalities was applied randomly to the skin overlying the right triceps surae muscle. After 15 minutes of cold application, mean skin temperatures recorded under WI, DI, and CGP decreased 12 degrees, 9.9 degrees, and 7.3 degrees C, respectively. The only significant differences in cooling were between WI and DI and between WI and CGP. Fifteen minutes after removal of the cold modalities, no significant differences were found in mean skin temperature between WI, DI, and CGP. The residual mean decrease in skin temperature between the pretreatment rest interval (time 0) and 15 minutes after removal of the cold modality (time 30) was significant for WI only. No cooling was demonstrated 1 cm proximal or distal to any of the cooling agents after 15 minutes of cold application. These findings provide valuable information for the use of cryotherapy in the clinical setting.

Adult↗
PubMed2001

[Microbiological quality of " ice, ice cream. sorbet" sold on the streets of Phnom Penh; April 1996-April 1997].

A study of the microbiological quality of ice lice creams/sorbets sold on the streets of Phnom Penh city was conducted from April 1996 to April 1997. Socio-demographic and environmental characteristics with two ice/ice creams samples were collected from vendors selected in the city. A total of 105 vendors and 210 ice/ice creams samples were randomly selected for the study period. Ice/ice cream vendors in the streets of Phnom Penh were adults (mean age: 28 years old) with a male predominance (86.5%). Mean educational level of vendors was 5 years with no training in mass catering. Most ice creams and sorbets (81.7%) were made using traditional methods. Microbiological analysis performed in the laboratory of Pasteur Institute of Cambodia indicated the poor bacteriological quality of the samples. The proportions of samples classified unsafe according to microbiological criteria were 83.3% for total bacterial count at 30 degrees C, 70% for total coliforms, 30% for faecal coliforms, 12.2% for Staphylococcus aureus and 1.9% for presence of Salmonella spp. These bacterial results suggest that many other food products sold in the streets may be similarly poor. Safety measures should be undertaken to avoid potential threats. Regulation of the street food sector should be part of a larger strategy for enhanced food safety and environmental quality in the city.

Cambodia↗