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PubMed1993

Hyaline membrane disease: the role of ethnicity and maternal risk characteristics.

OBJECTIVE: Our objective was to explore the association between maternal ethnicity and maternal antepartum complications of pregnancy, maternal sociodemographic factors, and newborn characteristics with the incidence of hyaline membrane disease. STUDY DESIGN: By using a retrospective cohort analysis the incidence of hyaline membrane disease was determined for 2295 preterm infants. The study population consisted of all live, inborn infants delivered vaginally from 1982 to 1987. Statistical differences were assessed by use of chi 2 and Student's t tests. A logistic regression procedure determined the relationship of ethnicity and hyaline membrane disease after the study was controlled for all other significant population differences. RESULTS: The differences between black and white populations in marital status, were statistically significant years of education, prolonged rupture of membranes, anemia, and chronic hypertension were statistically significant. Infants of black mothers were diagnosed with hyaline membrane disease less often than infants of white mothers (overall and at each gestational age interval). After the study was controlled for population differences, infants of black mothers were still found to experience hyaline membrane disease less often. CONCLUSION: These data suggest that hyaline membrane disease occurs less frequently, is less severe, and is accompanied by fewer related complications in black preterm infants.

Adolescent↗
PubMed1991

Protein-protein interactions and structural entities within the sea urchin extraembryonic matrix, the hyaline layer.

We have investigated the effects of a variety of experimental conditions on the structural integrity of the sea urchin extraembryonic matrix, the hyaline layer. Removal of Ca2+ resulted in the quantitative release of hyalin from isolated layers. Protein gel blot analyses indicated that, in the absence of Ca2+, hyalin was also quantitatively released from the layers surrounding 1-h-old embryos. However, no other polypeptides of the hyaline layer were released in significant amounts. The layers remaining after removal of hyalin were refractory to digestion with proteinase K and dissociated only in the presence of chaotropes. These results provide insights into the structural organization within the hyaline layer as well as the role of the embryonic cell surface in maintaining the structural integrity of this extraembryonic matrix.

Animals↗
PubMed1992

Biochemical basis for mouse resistance to hyaline droplet nephropathy: lack of relevance of the alpha 2u-globulin protein superfamily in this male rat-specific syndrome.

It is well-established that binding of a chemical to alpha 2u-globulin is the rate-limiting step in the development of male rat-specific hyaline droplet nephropathy. Mice synthesize mouse urinary protein (MUP), a protein which is very similar to alpha 2u-globulin, but this protein does not render the mouse sensitive to a similar renal toxicity. Therefore, the purpose of the present study was to determine the biochemical basis for mouse resistance to hyaline droplet nephropathy. Male Fischer 344 rats and B6C3F1 mice excreted 12.24 +/- 0.60 and 14.88 +/- 0.99 mg of alpha 2u-globulin and MUP daily, indicating that quantitative differences in protein excretion were not involved in the species specificity of the nephropathy. With d-limonene as a model hyaline droplet inducing agent, both rat and mouse liver microsomes oxidized the terpene to its 1,2-epoxide (the metabolite that binds reversibly to alpha 2u-globulin in vivo), demonstrating that metabolic differences do not determine the mouse resistance to this lesion. In spite of the formation of the epoxide intermediate, no binding of [14C]d-limonene equivalents to mouse kidney proteins was observed. In contrast, about 40% of the d-limonene equivalents in male rat kidney was reversibly bound to renal proteins. The renal reabsorption of alpha 2u-globulin and MUP was markedly different, as rats reabsorbed about 60% of the total filtered load of alpha 2u-globulin, but MUP was not reabsorbed by the mouse kidney. Given the absence of MUP in mouse kidney, in vitro equilibrium saturation binding studies were also conducted to determine whether MUP could bind the epoxide metabolite. alpha 2u-Globulin bound [14C]d-limonene-1,2-oxide with an apparent Kd of 4 x 10(-7) M. However, under identical experimental conditions, MUP failed to bind the epoxide. These data indicate that two major biochemical differences between alpha 2u-globulin and MUP contribute to mouse resistance to hyaline droplet nephropathy. Under both in vivo and in vitro conditions, MUP does not bind d-limonene-1,2-oxide, the rate-limiting step in the development of the nephropathy. However, even if MUP did bind the epoxide, the fact that it is not reabsorbed into the mouse kidney precludes its involvement in a syndrome involving renal protein overload. Finally, the absence of an interaction between d-limonene, a model hyaline droplet inducer, and the protein most similar to alpha 2u-globulin suggests that no other protein in the alpha 2u-globulin superfamily is likely to cause hyaline droplet nephropathy in other species.

Alpha-Globulins↗
PubMed1994

Hyaline body myopathy.

Muscle biopsy from two unrelated patients, a male aged 40 and a female aged 3, with relatively non-progressive limb weakness since infancy, revealed numerous subsarcolemmal glassy, hyaline appearing bodies present in 20-30% of the fibres. Type 1 fibre predominance was present, and the hyaline bodies were exclusive to type 1 fibres. The hyaline bodies were negative with oxidative enzyme and periodic acid-Schiff stains. Electron microscopy showed the hyaline bodies to contain amorphous granular material of unknown composition. No membrane separated the hyaline bodies from the surrounding sarcoplasm. Hyaline body myopathy most likely represents a distinct congenital myopathy because of its childhoot-onset, non-progressive course, and distinct morphological features.

Adult↗
PubMed1996

The hyaline zone and associated root surface changes in experimental orthodontics in rats: a light and scanning electron microscope study.

Light and scanning electron microscopic (SEM) examinations were used to study the hyaline zone and associated root resorption after orthodontic treatment of the upper first molars of rats. The orthodontic treatment consisted of a fixed buccal expansion appliance with an initial force of 250 mN. The animals were divided into nine experimental groups with orthodontic treatment and one control group without orthodontic treatment. Three groups were sacrificed immediately after 1, 3, and 7 days of treatment, and six groups after 7 days of treatment followed by 1, 2, 3, 4, 5, and 6 weeks without treatment. A hyaline zone on the pressure side of the mesial root was identified at the light microscopic level of examination as well as the SEM examination as early as 1 day after the initiation of treatment. In the SEM it was found that on the roots of teeth studied in the first few days after initiation of treatment the hyaline tissue was so firmly attached to the root that it remained in place during the extraction and preparation procedures. After longer treatment periods these hyaline zones were lost at extraction or during the preparation for the SEM examination. The cementum surface under the hyaline zone had a smooth appearance suggesting that the surface had been modified by substances released from the hyaline zone. After 1 week of treatment, resorption in the cementum could be noticed. The resorption extended to the dentine as uncovered dentinal tubuli were found. Formation of reparative cementum started two weeks after treatment. Changes in the cementum surface as well as root resorption cavities could be seen for as long as 6 weeks after the cessation of orthodontic treatment.

Activator Appliances↗
PubMed1994

Hyalinizing spindle and epithelioid cell nevus. A study of five cases of a distinctive histologic variant of Spitz's nevus.

Five cases are described of a distinctive histologic variant of benign spindle and epithelioid cell nevus characterized by extensive and prominent stromal hyalinization. The lesions consisted of a proliferation of spindle or epithelioid nevocytes scattered singly or in small clusters in the dermis and surrounded by abundant paucicellular hyalinized or collagenous stroma. Three patients were men and two were women. Their age range was 23 to 45 years (mean, 32). Two of the lesions were located in the head and neck region, two in the lower extremities, and one in the trunk. Immunohistochemical strains showed positive staining of the spindle or epithelioid cells with S-100 protein and vimentin; stains for keratin, EMA, CEA, actin, and desmin were all negative. Van Gieson and trichrome histochemical reaction demonstrated the collagenous nature of the hyalinized intercellular matrix; Congo red, crystal violet, and alcian blue stains were all negative. The etiology and pathogenesis of the intercellular hyalin deposits are unknown, but they probably represent a regressive phenomenon in longstanding or involuting lesions. Hyalinizing Spitz nevus must be included in the differential diagnosis of cutaneous lesions exhibiting a prominent hyalinized stroma and must be differentiated from other dermal neoplasms, particularly cutaneous metastases from occult internal malignancies and malignant melanoma.

Adult↗
PubMed1991

Rete testis hyperplasia with hyaline globule formation. A lesion simulating yolk sac tumor.

The presence of eosinophilic, hyaline globules in association with epithelial hyperplasia was noted in the rete testis of three patients with germ cell tumors. In the more florid examples, this proliferation formed a solid and microcystic pattern that, in association with the hyaline globules, mimicked a yolk sac tumor component. However, the bland cytologic features of the cells and the conformation to the configuration of the rete testis were keys to its reactive nature. A subsequent review of 48 testicular specimens containing well-defined areas of the rete testis showed hyaline globule formation in the rete testis or tubuli recti in 16 of 27 germ cell tumors, one of five other testicular tumors (four stromal tumors and one plasmacytoma), and none of 16 nonneoplastic cases. Many of the cases that had hyaline globules also showed epithelial hyperplasia. Further analysis demonstrated an incidence of rete testis invasion by neoplasm in cases that had hyaline globules, with or without epithelial hyperplasia, that was significantly higher (p less than 0.01) than that seen in neoplastic cases lacking hyaline globules. We concluded that this pseudoneoplastic reaction developed secondary to invasion of the rete testis by tumor. Immunostains supported the nonneoplastic nature of the proliferative lesions and indicated that the globules represented various proteins that had been absorbed from the lumen of the rete testis by the epithelial-lining cells but not successfully secreted.

Albumins↗
PubMed2004

Hyalinizing trabecular adenoma of the thyroid gland: cytologic features in 29 cases.

Cytologic material (fine-needle aspiration biopsy smears, thin-layer preparations, cell block sections, and touch preparations) from 29 cases of hyalinizing trabecular adenoma was studied to 1) establish the cytologic profile of the neoplasm and 2) compare the profile with those of papillary and medullary carcinoma. Papanicolaou- and Diff-Quik-stained smears of the hyalinizing trabecular adenomas were uniformly bloody. The smears featured cells in cohesive aggregates radially oriented around hyaline material, less frequently, singly. Cytoplasm was abundant, and the nucleocytoplasmic ratio was low. Intranuclear cytoplasmic inclusions, nuclear grooves, and nuclear overlapping were very common and best seen with the Papanicolaou method. Diff-Quik-stained smears highlighted the hyaline material (metachromatic), perinucleolar clearing, and cytoplasmic bodies. The combination of a bloody background, radially oriented cohesive cells, cells with abundant cytoplasm, nuclei with very frequent cytoplasmic inclusions and grooves, and the presence of hyalin should suggest the presence of hyalinizing trabecular adenoma.

Adenoma↗
PubMed1989

Electron microscopy of hyalin bodies in the human intraosseous endolymphatic sac.

Hyalin bodies are amorphous, eosinophilic masses protruding from the subepithelial connective tissue into the lumen of the intraosseous endolymphatic sac (ES). We studied hyalin bodies at the electron microscopic level. Celloidin- embedded temporal bone sections known to have hyalin bodies were re-embedded into plastic and cut into thin sections appropriate for electron microscopy. The results revealed that the hyalin bodies are composed predominantly of thick bundles of collagenous fibers arranged in various directions. Fibroblasts and disintegrated macrophages were occasionally observed among the collagen fibers. Concentric calcific structures found within the hyalin bodies were composed of multiple smaller, concentric, lamellar calcifications. The results of this study support the hypothesis that the hyalin bodies are a repository of membranous cellular debris phagocytized by the macrophages.

Calcinosis↗
PubMed2006

Hyalinizing trabecular adenoma of the thyroid revisited: a histologic and immunohistochemical study of thyroid lesions with prominent trabecular architecture and sclerosis.

BACKGROUND: Since its description, hyalinizing trabecular adenoma (HTA) of the thyroid has been a controversial entity. Some have considered it a unique entity, some have considered it a variant of papillary carcinoma (PC), and still others have considered it a nonspecific pattern that may be seen with a variety of thyroid lesions. Complicating the matter, studies demonstrating metastases have shown entities that do not appear to be HTAs as originally described, and molecular studies showing changes of PC have used methods that are not specific. This study reviews our experience with thyroid lesions that showed at least some histologic features of HTA and presents the immunohistochemical findings for these lesions using antibodies employed for the diagnosis of PC. DESIGN: Our files were reviewed for all thyroid resection reports describing lesions with hyalinized or sclerotic stroma and a trabecular architecture within the diagnosis or diagnostic comment. All cases were reviewed and classified as either HTA or as different lesions based upon histologic features. Immunohistochemistry with antibodies to HBME1, CK19 and p63 was performed with all lesions and with a series of controls. RESULTS: Eighteen thyroid lesions with prominent sclerosis or hyalinization and trabecular architecture were identified. Only 4 of these were found to completely match the histologic and cytologic descriptions of HTA by HE review. The other cases showed histologic features more compatible with other diagnoses including cellular adenomatoid nodule (5), follicular adenoma (4), follicular variant of PC (FVPC) (3), and epithelial neoplasm with features of FVPC (2). All HTAs lacked immunoreactivity for HBME1, CK19 and p63. All cases deemed to be adenomatoid nodules, follicular adenomas and epithelial neoplasms showed no immunoreactivity for HBME1 and CK19 and, of these, only a single AN showed immunoreactivity for p63. Cases deemed to be FVPCs showed diffuse immunoreactivity for HBME1 and CK19 and 1 reacted with antibodies to p63. Of control PCs and other thyroid lesions, reactivity for HBME1, CK19, and p63 was observed in 8/8, 7/7, and 7/8 and 3/27, 7/27, and 7/27 cases, respectively. CONCLUSIONS: A sclerotic or hyalinized stroma with a trabecular growth pattern may be seen in a number of different thyroid lesions and, when seen, is usually a focal feature of a lesion other than HTA. Immunohistochemistry may be of assistance as cases of FVPC with prominent hyalinization and trabeculation will show immunoreactivity for HBME1 and CK19, whereas HTAs and other thyroid lesions with hyalinization and trabeculation will not.

Adenoma↗
PubMed1985

Hyaline bodies in ameloblastoma: histological and ultrastructural observations.

An exceedingly rare case of ameloblastoma, plexiform type, which contained numerous hyaline bodies was reported. Hyaline bodies occurred within the parenchyma, and showed similar histochemical reactions to those of hyaline bodies in odontogenic cysts. Ultrastructurally, hyaline bodies found in the present case of ameloblastoma were composed of a peripheral electron-dense area and central electron-lucent area. Well-developed endoplasmic reticulum containing electronlucent and homogeneous substance were seen in the epithelial cells adjacent to hyaline bodies. Furthermore, electron-dense spherical granules, with or without limiting membrane, were scattered in the cytoplasm of epithelial cells. It is thought that hyaline bodies in the present case of ameloblastoma are particular products formed by odontogenic epithelial cells.

Adult↗
PubMed1977

Alcoholic hyalin, microfilaments and microtubules in alcoholic hepatitis.

The alcoholic hyalin which is composed of light and dark occasionally swollen and conglomerating filaments, is found to be surrounded by proliferated RER, hypertrophied Golgi apparatus and mitochondria containing enlarged matrical granules. Besides, microfilaments and some microtubules are seen in relation to the hyaline bodies. In biopsies where hyaline bodies are scarce, hepatocytes without alcoholic hyalin present similar changes and the Golgi apparatus is seen to contain very low density lipoprotein-like particles. These ultrastructural changes are suggested to be related to an early stage in the development of alcoholic hyalin. The abundance of microfilaments indicates an increased motility of the hepatocytes, possibly as a part of the regenerative processes. It can hardly be precluded that microfilaments disintegrate and accumulate in the alcoholic hyaline mass.

Adult↗
PubMed1991

Doppler assessment of pulmonary artery pressure and extrapulmonary shunting in the acute phase of hyaline membrane disease.

The natural history of pulmonary artery pressure and extrapulmonary shunting in acute hyaline membrane disease was studied by serial Doppler echocardiography in 57 preterm infants, 38 with, and 19 without, hyaline membrane disease. Pulmonary artery pressure was assessed non-invasively by its inverse relationship with the ratio of pulmonary artery Doppler time to peak velocity: right ventricular ejection time. The mean ratio was significantly lower in the infants with hyaline membrane disease. The mean ratio for each infant with hyaline membrane disease varied widely and did not correlate with criteria of maturity or severity of disease. Individual ratios correlated with arterial pH. Between 60-80 hours after birth, 14 of 18 infants with hyaline membrane disease (78%) and one of 19 without (5%) had patent ductus arteriosus. Left to right and bidirectional shunting at ductal and atrial level were common; pure right to left shunting was uncommon. The mean ratio seen with bidirectional shunting was significantly lower than that seen with left to right shunting. Ratios and patterns of extra pulmonary shunting were similar when the fractional inspired oxygen (FIO2) was greater than 0.9 compared with when it was less than 0.9. Pulmonary artery pressure is high during the acute phase of hyaline membrane disease but varies widely among infants. A few infants have extrapulmonary right to left shunting, and these infants are difficult to detect clinically.

Acute Disease↗
PubMed2004

Sertoli cell nodules in the undescended testis: a histochemical, immunohistochemical, and ultrastructural study of hyaline deposits.

AIMS: To document the morphology, immunohistochemical staining properties, and ultrastructural features of hyaline material in Sertoli cell nodules of undescended testis and contrast them with those of sex cord tumour with annular tubules (SCTAT), which is histologically similar. To highlight the need to distinguish these nodules from other Sertoli cell hyperplasias, such as intratubular Sertoli cell proliferations, which occur in specific clinical contexts. MATERIALS/METHODS: A retrospective study of 46 orchidectomy specimens from cryptorchid testes, 27 of which contained Sertoli cell nodules. Special histochemical stains, immunohistochemical stains for type IV collagen and fibronectin, and ultrastructural examination of the hyaline material were performed using tissue from paraffin wax embedded tissue blocks. RESULTS: The hyaline deposits in SCTAT and Sertoli cell nodules had similar staining patterns-periodic acid Schiff (PAS) and PAS-diastase positivity with variable staining of Martius scarlet blue and Masson trichrome. Type IV collagen immunoreactivity was seen in hyaline areas, although fibronectin was negative. Electron microscopy of hyaline areas confirmed a compact matrix identical to components of the basement membrane in the adjacent seminiferous tubules. CONCLUSION: This study describes an unusual form of Sertoli cell proliferation in undescended testes, which must be distinguished from Sertoli cell tumours and other forms of proliferation. In addition, the hyaline material within Sertoli cell nodules in the cryptorchid testis is histochemically, immunohistochemically, and ultrastructurally consistent with both matrix and fibrous components of seminiferous tubule basement membranes. Increased production of basement membrane material, with subsequent invagination into tubules, is the most likely origin of this material.

Adolescent↗
PubMed1987

Lung water and vascular permeability-surface area in premature newborn lambs with hyaline membrane disease.

Extravascular lung water and vascular permeability-surface area products were measured with a multiple indicator dilution method in 6 premature lambs with hyaline membrane disease 1-5 hours following delivery by cesarean section. The indicators used were 51Cr-labelled erythrocytes, 125I-albumin, 3H-water, and 14C-urea. Results were compared with previously obtained data in newborn lambs without hyaline membrane disease also delivered by cesarean section. Extravascular lung water was significantly higher in lambs with hyaline membrane disease [23.2 +/- 1.0 (SEM) vs. 10.7 +/- 1.4 ml/kg body wt]. Vascular permeability-surface area products for 14C-urea were significantly lower in lambs with hyaline membrane disease (0.30 +/- 0.10 vs 0.78 +/- 0.11 ml/s per kg). It is concluded that extravascular lung water is high in lambs with hyaline membrane disease. Permeability-surface area products for 14C-urea is low in lambs with hyaline membrane disease, which probably indicates a decrease in detectable surface area for exchange due to derecruitment or hypoperfusion of pulmonary exchange vessels in edematous and hypoxic areas of the lungs.

Animals↗
PubMed1983

Otosclerosis: relationship of spiral ligament hyalinization to sensorineural hearing loss.

The sensorineural component of a mixed hearing loss due to otosclerosis is generally accepted as due to the otosclerotic lesion. The existence of pure cochlear otosclerosis without stapes fixation has been questioned. However, we are documenting 7 such cases in a separate publication. Results of this study, which evaluates 46 temporal bones with clinical or cochlear otosclerosis, demonstrate that the degree of sensorineural loss is directly related to the amount of hyalinization of the spiral ligament. The hyalinization occurs adjacent to active otospongiotic lesions but not next to inactive otosclerotic lesions. Both types of lesions may involve the cochlear endosteum. Small channels through the endosteal bone from the lesion to the spiral ligament have been found. The hyalinization spreads laterally from these channels. The hyalinization is presumably a result of the passage of toxic substances (proteolytic enzymes) from the lesion to the ligament. Strial atrophy is most pronounced on ligaments with the greatest degree of hyalinization. Hyalinization in only one ear produces decreased hearing compared to the other ear.

Cochlea↗
PubMed1990

Hyaline membranes in postmature infants.

Hyaline membranes in 21 of postmature infant autopsies were studied, using 27 term infant autopsies as the control population. The occurrence of hyaline membranes was much higher in postterm (86%) than term infant autopsies (26%). Seventeen of 21 postmature infants had clinical and microscopic evidence of aspiration, contaminated by meconium in 14, suggesting that meconium and/or amniotic aspiration may be an etiologic factor in postmature hyaline membrane formation. Thirteen infants had severe hyaline membrane formation, microscopically distinguishable from hyaline membrane disease of the premature only by the maturity of the underlying lung. Besides the pulmonary findings, little difference in organ histology was observed between the two groups. This study showed that hyaline membrane formation resulted in asphyxia and respiratory failure in the majority of the postmature infants who were already troubled with hypoxia and a combined respiratory and metabolic acidosis secondary to meconium aspiration, and eventually led to death.

Brain↗
PubMed2006

[Hyaline membrane disease: mortality and maternal and neonatal risk factors].

OBJECTIVE: To identify maternal and neonatal risk factors associated with hyaline membrane disease and its mortality. PATIENTS AND METHOD: A case-control study with 41 newborns with hyaline membrane disease and 123 controls was made between September 2001 and February 2002 in the Hospital Civil de Guadalajara Dr. Juan I. Menchaca. The diagnosis of hyaline membrane disease was done with clinical and radiographic data. Maternal risk factors were obtained by direct interview with the mother; the newborn data were obtained of the clinical files. The association was measured with the odds ratios (OR) and the confidence interval of 95%. RESULTS: The frequency of hyaline membrane disease was of 6.8 per 1,000 live births. Mortality rate was of 2.8 per 1,000 live births. There were 17 deaths (41%). The risk factors associated with hyaline membrane were: illness during the first and second trimester of pregnancy, (OR: 3.28 [1.16-9.31]; OR: 8.88 [3.56-22.50], respectively), Apgar score lower than seven at the first minute (OR: 18.57 [4.50-88.67]), and masculine gender (OR: 2.58 [1.15-5.83]). CONCLUSIONS: Frequency and mortality were similar to the reported by other studies made in population samples. The exposure to illnesses during pregnancy and the low Apgar score were associated with the increased frequency of hyaline membrane disease.

Case-Control Studies↗