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PubMedDate not supplied

Characterization of human uterus-derived growth substances.

Human myometrium and endometrium extracts processed separately could stimulate tritiated-thymidine incorporation into the DNA of cells with the fibroblast, myoblast and osteoblast phenotype. This effect was dose-dependent. Mitogenic activity of myometrial and endometrial extracts was acid stable and sensitive to tryptic digestion. In addition, mitogens of myometrial and of endometrial extracts were retained and could be eluted by 80% acetonitrile over 0.1% trifluoroacetic acid from cartridges of octadecylsilyl-silica. Reverse-phase high performance liquid chromatography (r-HPLC) separated fractions of myometrial extracts with broad cell and preferential cell type specificity from fractions with inhibitory activity for detector cells. Reverse-phase HPLC revealed the presence in endometrial extracts of various fractions with mitogenic activity for all detector cells. These growth and inhibitory factors could mediate important autocrine, paracrine and/or endocrine regulatory processes of human uterus.

Animals↗
PubMedDate not supplied

[Deformation of lucerne root hairs caused by the growth substances and culture broth filtrates of Rhizobium meliloti].

Deformation of lucerne root hairs caused by the action of beta-indolylacetic acid (IAA) and alpha-naphthylacetic acid (NAA) differs from deformation induced by Rhizobium meliloti. High concentrations of IAA brought about abnormal deformation of root hairs wherease the action of average concentrations of IAA resulted in corkscrew-like winding and wavy structures. No deformation was observed under the action of low IAA concentrations or the cultural broth of Rhizobium. Infective filaments in root hairs were found only upon infection with pure cultures of Rhizobium.

Acetates↗
PubMed1989

Human uterus-derived growth substances for rat bone cells and fibroblasts.

The effects of extracts from normal human uteri on tritiated thymidine incorporation and cell number were studied in primary cultures of isolated fetal rat calvarial osteoblasts and fetal rat skin fibroblasts. In this study crude uterine extracts were shown to stimulate tritiated thymidine incorporation into cellular deoxyribonucleic acid in osteoblast-like and fibroblast-like cell cultures above controls levels. This effect, which began after 6 hours, was maximal after 24 hours of incubation. In addition, the number of osteoblast-like and fibroblast-like cells increased above control levels after 1, 3, and 5 days of incubation. The above effects were dose-dependent, and maximal stimulation of uterine extracts was comparable with the stimulation achieved by 10% fetal bovine serum. The degree of stimulation by uterine extracts, expressed as percent above controls, was not dependent on the concentration of fetal bovine serum in cell culture medium. The above results are consistent with the view that uterine extracts contain mitogen(s) for bone cells and fibroblasts. These mitogens could play an important role in bone physiology and uterine pathophysiology.

Adult↗
PubMed1968

Growth substances from Veratrum tenuipetalum.

Young leaves and buds of Veratrum tenuipetalum yielded non-indolic growth accelerators and inhibitors in the acidic ether fraction. The titer of accelerators decreased while the inhibitors increased as leaves matured. This was also true when comparing extracts of immature and fruiting inflorescences. Indole 3-acetic acid was at no time detected in leaf, bud, and inflorescence extracts but indole 3-acetyl-aspartic acid was readily recovered from all of these. The alkaline ether fraction of leaf base meristems, buds, roots, and rhizomes was rich in crystalline and amorphous alkaloids and phenolic acids. Reducing the quantity of major substances through crystallization, precipitation, and filtration permitted thin film chromatographic separation of the minor alkaloids and phenolic acids in the presence of the major ones. The unchromatographed mixture, and also certain of the purified major and minor alkaloids studied, strongly inhibited growth in germinating oat and winter rye seeds. In addition, profound changes in morphology and cytology of the seminal roots resulted. DNA disappeared partially to completely from affected tissue in 2 day germinated seeds.

Plant Extracts↗