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PubMed1989

A theoretical study of glucosamine synthase. Part I. Molecular mechanics calculations on substrate binding.

Glucosamine synthase transfers the gamma-amino group of glutamine to fructose, producing 1-glucosamine which is the key constituent of bacterial and fungal cell walls. In this study, model calculations were performed on substrate binding to the enzyme active site. Two models of the active site of glucosamine synthase were proposed, which assume two different sequences of aminoacids, Cys-Gly-Ile and Cys-Ala-Cys, the first one being the N-terminal sequence of the Escherichia coli enzyme. Several initial geometries were assumed for these tripeptides, the energy was then optimized by means of molecular mechanics. It has been found that the structure which is both energy optimal and satisfies the assumed cysteine sulphur arrangement consists of combinations of C7eq and C7ax conformations of single residues. Molecular mechanics calculations were then performed on glutamine and D-fructose-6-phosphate, which are the substrates of the enzymatic catalysis, and on their complex with the enzyme glutamine-binding site. The spatial configuration of the compounds under study, which is optimal as far as the reaction path is concerned, also turned out to be an energy minimum.

Alanine↗
PubMed1984

The use of fructose 1-phosphate to detect Hunter heterozygotes in fibroblast cultures from high-risk carriers.

Fibroblast cultures from 49 possible Hunter disease carriers were collected. These cultures were analysed for the incorporation of 35S-sulphate into acid mucopolysaccharides in the presence and the absence of fructose 1-phosphate. For 10 of these women more than one abnormal result was observed, when two or three cultures from each individual were tested. For six additional women only one abnormal result was found, when three cultures for each of these females were analysed. The implication that just one abnormal result indicates carriership stems from the observation that 24 out of 25 obligate carriers have been confirmed by this criterion (Tønnesen et al. 1983). By mean of the same criterion we have thus established carriership for 16 possible carriers. From genetic inference three additional carriers were found among the females showing normal results in the fibroblast cultures. As a test of the reliability of the method, analyses of the tested informative female offspring of Hunter carriers showed 20 of 38 informative females to be carriers.

Cells, Cultured↗
PubMed1983

Reliability of the use of fructose 1-phosphate to detect Hunter cells in fibroblast-cultures of obligate carriers of the Hunter syndrome.

Pulse-chase experiments measuring 35S-sulphate incorporation into acid mucopolysaccharides were performed in the presence and absence of fructose 1-phosphate on fibroblasts obtained from one skin-biopsy of 25 obligate Hunter carriers. The presence of fructose 1-phosphate significantly increased the accumulation of 35S-labelled acid mucopolysaccharides in fibroblast cultures of 23 obligate Hunter carriers. In one carrier, the accumulation of labelled acid mucopolysaccharides was significantly increased prior to the addition of fructose 1-phosphate, and in one of the 25 obligate carriers the 35S-sulphate incorporation was normal in the presence as well as in the absence of fructose 1-phosphate. Similar experiments performed on mixtures of Hunter cells and normal cells revealed that 20% Hunter cells should be present to obtain a significantly increased difference in between the incorporation in the presence and in the absence of fructose 1-phosphate. Fructose 1-phosphate had no effect on the accumulation of labelled mucopolysaccharides in fibroblast-cultures of seven women with no family history of mucopolysaccharidosis. The present results show that pulse-chase experiments measuring 35S-sulphate incorporation into fibroblasts, cultured in the presence of fructose 1-phosphate, can identify Hunter carriership, provided that the accumulation is normal prior to the addition of fructose 1-phosphate. Furthermore, 35S-sulphate incorporation in the absence of fructose 1-phosphate, higher than mean +4SD of normal control-fibroblasts indicates carriership.

Biopsy↗
PubMed1983

Reliability of the Tønnesen technique for the identification of Hunter carriers.

The procedure for the detection of Hunter carriers suggested by Tønnesen et al. (1982) was checked in different mixtures of normal and Hunter cells as well as by examination of five obligate and five potential Hunter carriers. In the presence of fructose 1-phosphate there was a strict correlation between the proportion of mutant cells in the fibroblast culture and sulphate accumulation, both in artificial cell mixtures and in native cell cultures of Hunter carriers. In all obligate heterozygotes studied, sulphate incorporation was increased by a factor of two. The new technique seems to be suitable for carrier diagnosis. Its limitations are discussed.

Biopsy↗
PubMed1983

Glucosephosphate-isomerase type Kaiserslautern. A new variant causing congenital nonspherocytic hemolytic anemia.

In a 13-year-old German girl a GPI deficiency was found to be the cause of a chronic nonspherocytic hemolytic anemia with recurrent hemolytic crises. The hemolytic crises usually occurred after a feverish infection. Only once did the patient require blood transfusion during a crisis. Examination of the family indicated that the patient is doubly heterozygous for the deficiency. The investigation of the biochemical properties of the deficient enzyme revealed an altered electrophoretic migration, a pronounced thermolability, an increased affinity for G-6-P and slightly changed pH optima for both substrates. The described properties of the deficient GPI indicate that we are dealing with a new variant designated GPI-Kaiserlautern.

Adolescent↗
PubMed1990

Anaerobic ATP provision, glycogenolysis and glycolysis in rat slow-twitch muscle during tetanic contractions.

Rat soleus muscles were tetanically stimulated in situ with an occluded circulation to examine anaerobic adenosine triphosphate (ATP) provision and the regulation of glycolytic ATP production. Soleus muscles were stimulated for 30-60 s at 1 Hz with 100-200 ms trains (40-80 Hz). Muscles were sampled pre- and post-stimulation for measurements of pH, high energy phosphates and glycolytic intermediates. Total ATP provision by the slow oxidative fibres was 65-121 mumol/g dry muscle and 27-35% of the amount produced by fast glycolytic fibres. Contributions to total ATP provision in the initial 30 contractions were: phosphocreatine, 71%; glycolysis, 28%; and endogenous ATP, 1%. Following 60 contractions the contributions were 45-54%, 44-51% and 2-4%, respectively. During the initial 30 contractions, glycogenolysis (phosphorylase activity) and glycolysis [phosphofructokinase (PFK) activity] were similar as glucose-6-phosphate (G-6-P) and fructose-6-phosphate (F-6-P) did not accumulate. Small accumulations of PFK deinhibitors inorganic phosphate, adenosine diphosphate, adenosine monophosphate and fructose-1,6-diphosphate appeared to account for the PFK activity. In the final 30 contractions, phosphorylase activity increased above PFK as G-6-P and F-6-P accumulated. PFK activity and glycolytic ATP production also increased despite increasing hydrogen ion concentration [H+]. During intense tetanic stimulation of soleus muscle, glycolytic ATP production is initially limited by a low glycogen phosphorylase activity. The activity of PFK increases during in situ contraction through the accumulation of deinhibitors, despite increasing [H+].

Adenosine Triphosphate↗
PubMed1976

Purification and properties of two fructose-6-phosphate phosphoketolases in Bifidobacterium.

Fructose-6-phosphate phosphoketolase was purified from type strains of two species of the genus Bifidobacterium: B. globosum and B. dentium. The first species has a preferred "animal" habitat, like feces of animals and rumen of cattle; the latter is harboured in "human" habitat, like feces and dental caries of man. Two electrophoretic types of phosphoketolase (F6PPK) were previously distinguished and called "animal" and "human" type according to the habitat of the bifid organism. The purified preparations of these two phosphoketolases displayed very different optimum pH range, metal activator and molecular weight; outstanding difference was found in the substrate specificity: the enzyme from B. globosum was able to split xylulose-5-P as well as fructose-6-P, whereas the phosphoketolase from B. dentium appeared to be specific for fructose-6-P.

Actinomycetales↗
PubMed1980

Regulation of chitin synthesis during germ-tube formation in Candida albicans.

The synthesis of chitin during germ-tube formation in Candida albicans may be regulated by the first and last steps in the chitin pathway: namely L-glutamine-D-fructose-6-phosphate aminotransferase and chitin synthase. Induction of germ-tube formation with either glucose and glutamine or serum was accompanied by a 4-fold increase in the specific activity of the aminotransferase. Chitin synthase in C. albicans is synthesized as a proenzyme. N-acetyl glucosamine increased the enzymic activity of the activated enzyme 3-fold and the enzyme exhibited positive co-operativity with the substrate. UDP-N-acetylglucosamine. Although chitin synthase was inhibited by polyoxin D (Ki = 1.2 microM) this antibiotic did not affect germination. During germ-tube formation the total chitin synthase activity increased 1.4-fold and the expressed activity (in vivo activated proenxyme) increased 5-fold. These results could account for the reported 5-fold increase in chitin content observed during the yeast to mycelial transformation.

Candida albicans↗
PubMed1975

Allosteric activation and competitive inhibition of yeast phosphofructokinase by d-fructose.

Purified phosphofructokinase from bakers yeast is activated by D-fructose in low concentrations (up to 1 mM) and inhibited by high concentrations. The stimulatory effect of D-fructose is similar, but smaller than that of AMP. In the presence of AMP (0.4 mM or higher) D-fructose does no longer stimulate, but its inhibitory effect persists (KI = 8 mM). Its dualistic action on phosphofructokinase activity indicates that D-fructose might induce low frequency in glycolytic oscillations by direct interaction with the enzyme.

Adenosine Monophosphate↗
PubMed1975

Enzymes of D-fructose catabolism in species of Beneckea and Photobacterium.

Cell-free extracts of strains representative of the genera Beneckea and Photobacterium catalyzed a P-enolpyruvate dependent phosphorylation of D-fructose. The resulting product, fructose-1-P, was converted to fructose-1,6-P2 by 1-P-fructokinase. Both activities were inducible, being present in D-fructose-grown cells and reduced or absent in D-gluconate-(or succinate-) grown cells.

Adenosine Triphosphate↗
PubMed1977

Different degradation pathways for glucose and fructose in Rhodopseudomonas capsulata.

In Rhodopseudomonas capsulata the enzymes of the Entner-Doudoroff pathway and the Embden-Meyerhof pathway have been examined. Fructose-grown cells contained inducible activities of phosphoenolpyruvate-fructosephospho-transferase and 1-phosphofructokinase and only low levels of fructokinase and 6-phosphofructokinase. Although fructose-grown cells contained, in addition, all the enzymes of the Entner-Doudoroff pathway together with fructose-1,6-diphosphatase and phosphoglucose isomerase, the Entner-Doudoroff pathway was not operative in fructose catabolism and served only the degradation of glucose. The functional separation of glucose and fructose catabolism via the Entner-Doudoroff and a modified Embden-Meyerhof pathway, respectively, was confirmed by different approaches: 1. Radiorespirometric experiments with glucose and fructose labelled in positions 1, 2, 3, 3+4 and 6 have been carried out. The pattern of 14CO2-evolution from position-labelled glucose was characteristic for the Entner-Doudoroff pathway, that from position-labelled fructose for the Embden-Meyerhof pathway. 2. In the presence of arsenite up to 50% of glucose- and fructose-carbon was excreted as pyruvate. Using 1-14C-glucose, 86% of the pyruvate was labelled in the carboxyl group, whereas using 1-14C-fructose only 19% of the pyruvate was labelled in the carboxyl group. 3. A glucose-6-phosphate dehydrogenase-deficient mutant was isolated which lacked a functional Entner-Doudoroff pathway but which was unaltered in its ability to grow on fructose.

Fructose↗