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PubMed1950

[Complexones].

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Acetates↗
PubMedDate not supplied

[A method of fluorescence immunoassay with temporal resolution and the use of europium label and polymeric complexon].

Recently developed solid-phase immunofluorescent assay with temporal distinction involved a newly produced procedure for binding of of the boron group label with antibodies via modified polymer, which is able to bind with antibodies across the avidin bridge. The assay developed is more simple than the widely used procedures, it exhibits high sensitivity being superior as compared with a corresponding immunoenzyme assay by a decimal order.

Animals↗
PubMedDate not supplied

[Transport and metabolism of a membrane-active complexon in the mouse body].

Two-phase decrease in content of N-(3H-aminoacetyl)-I-aza-4,7,10,11-tetraoxacyclopentadecane hydrochloride (ATCH) and of its metabolites as well as absence of accumulation were observed in mice tissues. Kinetic parameters of 3H-products elimination estimated by means of sequental logarithmation enabled to suggest that ATCH and its free metabolites were intensively involved in biotransformation of molecules. 3H-glycine was found not to be a product of the ATCH metabolism. High and stable level (from 10 min up to 24 hrs) of water soluble and protein-bound derivatives of ATCH were found in mice liver tissue and blood plasma after intravenous administration of the macroheterocycle.

Animals↗
PubMed1977

[Effect of antioxidants and complexons on mitochondrial swelling induced by thyroxine].

The rate of thyroxin-induced swelling of rat liver mitochondria depended but little on the cation compositions of the medium (KCl, NaCl or choline chloride), this indicating that the enhancement of mitochondrial membrane permeability for cations in the presence of thyroxin was of negligible selectivity. The antioxidants alpha-tocopherol and beta-ionol in concentrations completely inhibiting the lipid perioxidation failed to affect the thyroxin-induced mitochondrial swelling; hence the latter could not be attributed to the lipoperoxidation. The kinetics of the swelling and the fact of its inhibition by EGTA imply the necessity of calcium ions in the activation of the process and make possible a postulation that the thyroxin-based mitochondrial swelling was based on the process of activation of the membrane phospholipase in these organellae.

Animals↗