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At least 91 records · Page 5Linked to original sources
PubMed2003

Laryngotracheal injury after percutaneous dilational tracheostomy in cadaver specimens.

OBJECTIVE: To evaluate the stoma and surrounding insertion site for common laryngotracheal injury patterns after percutaneous dilational tracheostomy that may contribute to clinically significant tracheal stenosis. STUDY DESIGN: Preliminary prospective cadaver study. METHODS: A preliminary prospective cadaver study was performed in which percutaneous dilational tracheostomy (Ciaglia Blue Rhino kit) was placed in six fixed cadaveric specimens. Laryngotracheal segments were harvested. Two independent evaluators graded cadaver features and characteristic injuries in laryngotracheal specimens the tracheal site of stoma placement was identified. Cadaver features evaluated included gender, obesity, and ease of landmark identification. Mucosal and cartilage injuries were evaluated on a scale of 0 to 4, with 4 representing severe comminuted injuries beyond the intended stoma site. RESULTS: Accurate prediction of tracheal placement was achieved in only three (50%) of the specimens. In the remaining cadavers, one stoma site was placed higher than intended (16%) and two sites were lower than intended (33%). Mucosal injury was most severe at the anterior internal surface of the trachea with all specimens sustaining tears beyond one tracheal ring (mean mucosal injury score, 3.5). Cartilaginous injury was severe in five of six specimens (83%) that sustained multiple comminuted injuries to two or more adjacent rings (mean cartilage injury score, 3.34). Cricoid comminutions and a posterior membranous tracheal wall injury were each found in one specimen. CONCLUSIONS: Laryngotracheal injuries found after percutaneous dilational tracheostomy in the study indicated that severe damage to mucosa and cartilage surrounding the intended stoma site occurs at the time of placement. These injuries may contribute to clinically significant tracheal stenosis preventing decannulation in patients undergoing percutaneous dilational tracheostomy. Two case studies that support this hypothesis are also presented.

Adult↗
PubMed2000

Immunologic and nonimmunologic factors: different risks for cadaver and living donor transplantation.

BACKGROUND: There is a debate about the relative contribution of immunologic (rejection) and nonimmunologic (limited nephron mass) factors in long-term graft survival. METHODS: Using multivariate analysis, we studied the association of the following variables with outcome: delayed graft function (DGF), acute rejection, recipient race (black vs. nonblack), donor age (<50 vs. > or =50), donor race, and donor and recipient gender. Because of the association between DGF and rejection, recipients were grouped as follows: DGF, rejection; DGF, no rejection; no DGF, rejection; no DGF, no rejection. Data were analyzed on 1199 first kidney transplants in adults (752 living donor, 447 cadaver donor) done between January 1, 1985 and December 31, 1996. Two analyses were done: first, all transplants; second, only those with > or =1 year survival. For both, there was no difference in risk factors if death with function was or was not censored. RESULTS: For all cadaver transplant recipients, risk factors were acute rejection, DGF plus rejection, black recipient race, and donor age > or =50. For living donor recipients, only acute rejection was a risk factor. When only 1-year graft survivors were considered, risk factors were the same: for cadaver recipients, risk factors were acute rejection, DGF plus rejection, black recipient race, and donor age > or =50; for living donor recipients the risk factor was rejection. CONCLUSION: We found immunologic factors (rejection with or without DGF) to be significant in both living donor and cadaver donor transplants. Nonim. munologic factors (donor age, recipient race) were significant only in cadaver donor transplants.

Acute Disease↗
PubMed2006

A cadaver study in preparation for facial allograft transplantation in humans: part II. Mock facial transplantation.

BACKGROUND: The authors have performed mock facial transplantation by harvesting total facial-scalp flaps from donors and transferring them to recipient cadavers. METHODS: A total of 10 fresh human cadavers were dissected. In the donor, the authors have measured the time of facial-scalp flap harvesting, and the length of the arterial and venous pedicles and sensory nerves that were included in the facial flaps. In the recipient, the authors have evaluated the time of facial skin harvest as a monobloc full-thickness graft, the anchoring regions for the inset of the donor facial flaps, and the time sequences for the vascular pedicle anastomoses and nerve coaptations. RESULTS: In the donor cadaver, the mean harvesting time of the total facial-scalp flap harvest was 235.62 +/- 21.94 minutes. The mean length of the supraorbital, infraorbital, mental, and great auricular nerves was 1.5 +/- 0.15, 2.46 +/- 0.25, 3.02 +/- 0.31, and 6.11 +/- 0.42 cm, respectively. The mean length of the external carotid artery, the facial vein, and external jugular vein was 5 +/- 0.32, 3.15 +/- 0.32, and 5.78 +/- 0.5 cm, respectively. In the recipient cadaver, the mean harvesting time of facial skin as a monobloc full-thickness graft was 47.5 +/- 3.53 minutes. The mean time for the preparation of the arterial and venous pedicles and sensory nerves for the future anastomoses and coaptation was 30 +/- 0 minutes. The mean time for facial flap anchoring was 22.5 +/- 3.53 minutes. The total mean time of facial mock transplantation without vessels and nerve repair was 320 +/- 7.07 minutes (5 hours 20 minutes). CONCLUSION: Based on anatomical dissections in this cadaver study, the authors have estimated the time and sequence of facial flap harvest and inset to mimic the clinical scenario of the facial transplantation procedure.

Aged↗
PubMed2006

Histomorphometric analysis of bone density in the maxillary tuberosity of cadavers: a pilot study.

BACKGROUND: Maxillary tuberosities have been widely used as a source of autogenous bone for a variety of oral surgical procedures. However, no previous studies have actually demonstrated the histologic and histomorphometric characteristics of this anatomical region in detail. The aim of this study was to evaluate maxillary tuberosities in cadavers histologically and histomorphometrically. METHODS: A block section, including the overlying alveolar mucosa, was obtained from a randomly assigned maxillary tuberosity of 20 cadavers (nine females and 11 males). All cadavers were edentulous distal to the second bicuspid teeth, and 14 out of 20 were edentulous distal to the cuspids. A minimum of 35 serial sections were obtained from each cadaver. Description analysis was performed for the presence of either a fibrotic or fatty infiltration of the marrow spaces and thickness and continuity of alveolar bone. Computer-based histomorphometric image analysis of the percentage surface area of bone was also carried out in three sections of each cadaver. Statistical analysis was performed to establish possible differences between genders. The analysis did not control for nutritional status and/or possible systemic or skeletal diseases. RESULTS: Little variation existed between specimens, regardless of gender, with respect to thickness of oral mucosa overlying the tuberosity. Descriptive bone analysis revealed thin cortical bone and sparse cancellous bone patterns. In addition, the block specimens exhibited no evidence of osteoid or active bone formation, but large marrow spaces infiltrated with loosely organized fibrous connective tissue and/or lipid cells. Histomorphometric analysis demonstrated a mean percentage of vital bone of 24.23% +/- 5.2%. Stratification of the data revealed statistically significant differences in mean percentage of vital bone between genders (27.15% +/- 4.7% for males versus 20.66% +/- 3.4% for females; P = 0.003). CONCLUSIONS: Within the limitations of this study, maxillary tuberosities seem to mainly consist of marrow spaces, adipose tissue, and a low vital bone profile. Females demonstrated a statistically significant lower amount of vital bone than males. The results suggest that this specific area may not be an ideal source of autogenous bone for grafting purposes in older individuals. However, it is imperative to note that this investigation did not control for nutritional imbalances and skeletal disorders. Further studies are necessary to control all confounding factors.

Bone Density↗
PubMed1998

The vastus medialis oblique muscle and its relationship to patellofemoral joint deterioration in human cadavers.

Patellofemoral joint deterioration (PFJD) is frequently seen in physical therapy clinics and represents a significant problem for both patients and rehabilitation clinicians. The vastus medialis oblique (VMO) muscle is reported to be the primary stabilizer of the patella during knee extension. Most studies and treatment protocols emphasize strengthening of the VMO as the nonsurgical treatment of choice for patients with PFJD. The purpose of this study was to determine whether any relationship exists between the morphology of the VMO and the presence and severity of PFJD in human cadavers. Dissection of 374 vastus medialis (VM) muscles and patellofemoral joints was performed on 229 human cadaver lower limbs to determine what relationships exist between gender, VMO features, and PFJD. Patellofemoral joint deterioration was determined by direct visual observation and assigned a score based on severity of joint deterioration present. Two-way chi-square tests were performed to determine the relationships between cadaver gender, the presence of VMO features, and the presence and severity of PFJD. Linear regression was performed to determine whether any correlation existed between the VMO fiber angle and the severity of PFJD. A one-way analysis of variance was performed to determine whether any differences existed between the VMO fiber angle and the PFJD groups. No statistically significant relationships, correlation, or differences existed in any of the tests performed between cadaver gender, VMO features, and presence or severity of PFJD. The presence or severity of PFJD in human cadavers is not related to either gender or VMO morphologic features. The results of this study do not support the premise that a more distal insertion of the VMO onto the patella of the VMO will have any effect on the presence or severity of PFJD.

Adult↗
PubMed2004

Morphological study of the uncommon rectus sterni muscle in German cadavers.

OBJECTIVE: The present investigation has been designed to study the incidence of the rectus stern muscle in German human cadavers dissected in the Kingdom of Saudi Arabia, trying to find a postulation for the development of such muscle when present. DESIGN: Gross dissection of 130 cadavers, of both sexes, was performed throughout a period of 10 years. SETTING: Department of Anatomy College of Medicine, King Faisal University, Dammam, Saudi Arabia. INTERVENTION: Investigation of the origin and insertion of the rectus sterni and measurements of its length and width. RESULTS: Two adult cadavers, one of each sex, had shown well-developed bilateral rectus stern muscles. All muscles identified were parasternal in position, being superficial to the medial portion of the pectoralis major muscle. Minor morphological differences were observed among the four muscle masses concerning their length, breadth, origin and insertion. CONCLUSION: The current study has determined the incidence of the rectus sterni muscle, in German cadavers to be 1.54% per bodies examined compared to 4% in cadavers from Saudis. Such a frequency is compared to that reported in different geographic populations. The rectus sterni muscle is innervated by the anterior cutaneous branches of the intercostal nerves. The description of the rectus sterni muscle and its incidence determined in the present study, might be of a great help for clinicians radiographing or tackling the pectoral region.

Adult↗
PubMed1993

Extracorporeal support with a cadaver liver as a bridge to transplantation.

Extracorporeal liver perfusion (ECLP) has been used for detoxifying blood in patients in class IV hepatic encephalopathy. Palliation of the moribund patient utilizing extracorporeal devices with cross-circulation of a cadaver liver has been documented for over three decades. Common problems associated with this procedure which appear in the literature include cadaver liver distention, increased resistance to blood flow, and limited time of extracorporeal support due to cadaver liver failure. This report summarizes the experiences of the perfusion team in utilizing an extracorporeal circuit with an otherwise nontransplantable cadaveric liver, to support the decompensating hepatic patient as a bridge to transplantation. Between January and July 1992, three patients were supported for hepatic failure with ECLP. Two patients were placed on ECLP with a modified circuit containing two positive displacement pumps and one centrifugal pump. The third patient was placed on ECLP with a circuit that contained two centrifugal pumps and one positive displacement pump. Patient age ranged from 6 to 38 years and length of support ranged from 24 to 72 hours. In all three patients, a centrifugal pump was placed in the suprahepatic inferior vena cava line to facilitate cadaver liver drainage and decompression. Intensive monitoring of both patient and cadaver liver hemodynamics, hepatic function, and hematological status was performed. All three patients were successfully weaned from ECLP. Two patients received successful orthotopic liver transplantation. The third died of complications unrelated to ECLP after support was discontinued.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed2005

The new laparoscopic proctocolectomy training (in soft cadaver).

OBJECTIVES: The purpose of the present study was to evaluate the quality of preservation (tissue plane, named vessels identification, consistency of colon and rectum), quality of performing procedures, difficulties and problems and finally the satisfaction of surgeons in laparoscopic proctocolectomy in soft cadaver. SETTING: Colorectal Division, Department of Surgery and Surgical Training Center Department of Anatomy, Faculty of Medicine, Chulalongkorn University. DESIGN: Prospective descriptive study MATERIAL AND METHOD: 10 soft cadavers were scheduled for laparoscopic proctocolectomy. The procedures (colon-rectum mobilization and named vessels identification) were performed by 14 experienced surgeons (8 colorectal surgeons) and assisted by surgical residents. The quality of preservation, successfulness and the satisfaction in performing the procedures were recorded using questionnaires for evaluation. RESULTS: The preservation was very good in every aspect especially tissue plane between colon, mesocolon and retroperitoneum which was clearly dissected, same asfasciapropria of rectum. The named vessels and the tissue consistency were very well preserved and tolerated to laparoscopic equipment handling. The surgeons were satisfied with the tissue handling and dissections. There were two difficulties, the first was air leakage but simply corrected with purse string suture and the second was unflavored smell which was not concerned. Laparoscopic proctocolectomy could be completely performed in soft cadaver. CONCLUSION: Laparoscopic proctocolectomy could be performed in soft cadavers with great satisfaction. Repeated practice is possible, so the surgeons can gain their experiences outside the operating theatre. This success may shorten the learning curve and may be the new era in cadaver-based training.

Attitude of Health Personnel↗
PubMed2005

Minimally invasive surgery training in soft cadaver (MIST-SC).

OBJECTIVES: The purpose of this study was to evaluate the surgical anatomy, tissue plane, organ consistency of soft cadaver and the possibility of minimally invasive surgery training in soft cadaver. SETTING: Surgical Training Center. Department of Anatomy and Department of Surgery Faculty of Medicine, Chulalongkorn University. DESIGN: Prospective descriptive study. MATERIAL AND METHOD: 2 soft cadavers were scheduled for fully laparoscopic surgery in upper gastrointestinal, colorectal, hepatopancreatobiliary and solid organs surgery. All the procedures were performed by the experienced surgical staffs and assisted by surgical staffs and/or surgical residents. The surgical anatomy, tissue plane, organ consistency and the satisfactory in performing the procedures were recorded for evaluation. RESULTS: The surgical anatomy, the tissue consistency the anatomical plane were very well preserved with mean score of 4.72 +/- 0.45. All the surgeons were satisfied with the findings, the mean score was 4.97 +/- 0.18. All the plan procedures were completely performed with great satisfactory results. CONCLUSION: The Minimally Invasive Surgery Training in Soft Cadaver (MIST-SC) was feasible with great satisfactory. This successful integration of basic and advanced laparoscopic procedures into the soft cadaver setting would be the next step in evolution of MIS training.

Cadaver↗
PubMed1977

Donor pretreatment in cadaver renal transplantation.

Excellent cadaver allograft survival has been achieved with kidneys from cadaver donors pretreated with high intravenously given dosages of cyclophosphamide and intravenous injections of methylprednisolone. To test the efficacy of this method of pretreating the donor, allograft survival in 21 patients, group A, receiving kidneys from cadaver donors pretreated with 4 grams of cyclophosphamide and 4 to 6 grams of methylprednisolone given intravenously was compared with two other groups of cadaver allograft recipients, 39 patients, 23 group B patients receiving untreated kidneys harvested at the Mayo Clinic and 16 group C patients receiving untreated kidneys harvested elsewhere. Two year actuarial allograft survivors were 16 of 21 in group A, 13 of 23 in group B and seven of 16 in group C. We believe that cadaver donor pretreatment improves allograft survival significantly.

Adolescent↗
PubMedDate not supplied

Noncontact laser photothermal keratoplasty. II: Refractive effects and treatment parameters in cadaver eyes.

BACKGROUND: Noncontact laser photothermal keratoplasty may provide a new alternative for the treatment of myopia, hyperopia, and astigmatism. The purpose of this article is to study the refractive effect that laser photoablation keratoplasty is capable of producing on a normal human cadaver cornea, including the relationship between the keratometric changes and laser treatment parameters. METHODS: The human cadaver eyes were treated with a holmium laser (pulsed Ho:YAG, 2.10 microns, 250 microseconds) coupled to a maskable, polyprismatic delivery system mounted on either an optical bench or a slit-lamp microscope. Using a topographic videokeratography system, we first investigated the refractive effect that noncontact laser photothermal keratoplasty would produce on a normal cadaver cornea. We then studied the keratometric changes produced by different radiant exposure levels at a fixed treatment pattern, as well as by different treatment patterns at a fixed radiant exposure level. Finally, we studied the possible therapeutic application of laser photothermal keratoplasty for correcting high postoperative astigmatism on a cadaver eye model. RESULTS: For the single-pulse 3-millimeter ring of eight-spot treatment, the keratometric power of the cornea initially increased with the radiant exposure and peaked at 26 J/cm2. The refractive effect was increased by projecting an additional set of eight spots equidistant between the first eight spots on the same diameter ring. Eighteen J/cm2 was the minimal radiant exposure required to produce consistent and predictable keratometric changes. The corneas were flattened using treatment patterns smaller than or equal to 3 mm in diameter and steepened using treatment patterns larger than or equal to 5 mm in diameter. A transition zone between 4 and 5 mm was observed in which minimal and unpredictable keratometric changes of the central cornea occurred. The surgically-induced astigmatism (> 10.00 D) was corrected by progressive laser photothermal keratoplasty treatments. CONCLUSIONS: Laser photothermal keratoplasty can acutely steepen and flatten the cornea in human cadaver eyes.

Astigmatism↗
PubMed1996

Cadaver skin allografts and transmission of human cytomegalovirus to burn patients.

BACKGROUND: The potential role of cadaver skin as a vehicle for CMV transmission to burn patients has never been clearly defined. We sought to determine if a cytomegalovirus (CMV)-positive cadaver allograft transmits CMV infections to CMV-seronegative burn patients. STUDY DESIGN: All patients in this study were CMV seronegative on admission. They received CMV-seronegative blood products, and cadaver allografts for temporary wound closure and management without regard to the donor's CMV serum status (positive or negative). RESULTS: Of 493 patients admitted from 1989 to 1993, 22 were CMV seronegative on admission and required cadaver allografts for their burn wounds. Five (22.7 percent) of 22 patients seroconverted during hospitalization: one of five had CMV pneumonia develop, two had CMV viruria develop, and three had persistent fever, abnormal liver enzymes, and diarrhea not ascribable to bacterial or other viral agents. CONCLUSIONS: Cytomegalovirus infections result from using CMV-seropositive cadaver allografts on seronegative burn patients.

Adolescent↗
PubMed1997

Movements of vertebrae during manipulative thrusts to unembalmed human cadavers.

OBJECTIVE: The purpose of this study was to measure the relative movements of vertebrae during manipulative thrusts to unembalmed post-rigor mortis human cadavers. SETTING: The investigation was conducted in the gross anatomy laboratory at the University of Calgary. SUBJECTS: Two 77-yr-old, unembalmed, post-rigor mortis, male cadavers were used. INTERVENTIONS: The movements of vertebrae were investigated by using high-speed cinematography to record the movements of bone pins threaded into T10, T11 and T12 during spinal manipulative therapy to unembalmed post-rigor human cadavers. A single clinician delivered a series of posterior-to-anterior (p-to-a) thrusts to the right transverse process of either T10, T11 or T12, using a reinforced hypothenar contact. Relative p-to-a and lateral translations, as well as axial and sagittal rotations, in T10-T11 and T11-T12 were calculated. Corresponding p-to-a forces exerted by the clinician onto the cadaver were recorded using a pressure pad. MAIN RESULTS: Significant relative movements were measured primarily between the targeted and immediately adjacent vertebrae during the thrusts. Vertebral pairs remained slightly 'hyper-extended' after the rapid thrusts to T11, when the p-to-a forces returned to preload levels. CONCLUSIONS: These findings may be useful for the understanding of the deformation behavior of the vertebral column during therapeutic manipulation. A fully three-dimensional analysis of all six degrees of freedom, using a larger number of unembalmed cadavers, would be useful in clarifying the relationship between the externally applied forces and the observed relative movement patterns of the vertebrae.

Aged↗
PubMed2006

Sequential monitoring of burials containing large pig cadavers using ground-penetrating radar.

Ground-penetrating radar (GPR) was used to monitor 12 pig burials in Florida, each of which contained a large pig cadaver. Six of the cadavers were buried in sand at a depth of 0.50-0.60 m, and the other six were buried at a depth of 1.00-1.10 m and were in contact with the upper surface of a clay horizon. Control excavations with no pig internment were also constructed as blank graves and monitored with GPR. The burials were monitored with GPR for durations of either 12-13 or 21-21.5 months when they were then excavated to correlate the decomposition state of the cadaver with the GPR imagery. Overall, cadavers in sand were easily detected for the duration of this study at 21.5 months, even when completely skeletonized. Conversely, in clay it became increasingly difficult to image the pig cadavers over the first year of burial, even when they still retained extensive soft tissue structures.

Animals↗
PubMed1986

Body composition of two human cadavers by neutron activation and chemical analysis.

In vivo neutron activation analysis (NAA) is currently used to measure body composition in metabolic and nutritional studies in many clinical situations, but has not previously been validated by comparison with chemical analysis of human cadavers. Total body nitrogen (TBN) and chlorine (TBCl) were measured in two human cadavers by NAA before homogenization and chemical analysis (CHEM) after (cadaver 1: TBN, 1.47 NAA, 1.51 CHEM; TBCl, 0.144 NAA, 0.147 CHEM; cadaver 2: TBN, 0.576 NAA, 0.572 CHEM; TBCl, 0.0227 NAA, 0.0250 CHEM). The homogenates were also analyzed by NAA, and no significant differences were found, indicating that the effects of elemental inhomogeneity on the measurement of TBN and TBCl are insignificant. Total body water, fat, protein, minerals, and carbohydrates were measured chemically for each cadaver and compared with estimates for these compartments obtained from a body composition model, which when used in vivo involves NAA and tritium dilution. The agreement found justifies the use of the model for the measurement of changes in total body protein, water, and fat in sequential studies in groups of patients.

Activation Analysis↗
PubMed2005

Origin of lumbar spinal roots and their relationship to intervertebral discs: a cadaver and radiological study.

Previous studies on the anatomy of the lumbar spine have not clarified the precise relationship of the origin of the lumbar roots to their corresponding discs or their angulation to the dural sac. We studied 33 cadavers (25 formalin-preserved and eight fresh-frozen) and their radiographs to determine these details. All cadavers showed a gradual decrease in the angle of the nerve root from L1 to S1. The origin of the root was found to be below the corresponding disc for the L1 to L4 roots. In the formalin-preserved cadavers 8% of the L5 roots originated above, 64% below and 28% at the L4/L5 disc. In the fresh cadavers the values were 12.5%, 62.5% and 25%, respectively. For the S1 root 76% originated above and 24% at the L5-S1 disc in the formalin-preserved cadavers and 75% and 25%, respectively, in the fresh cadavers.A herniated disc usually compresses the root before division of the root sleeve. Thus, compression of the thecal sac before the origin of the root sleeve is common for L1 to L5 whereas compression at the root sleeve is common for S1. Our findings are of value in understanding the pathophysiology of prolapse of the disc and in preventing complications during surgery.

Adult↗
PubMed2005

Epidural migration of new methylene blue in 0.9% sodium chloride solution or 2% mepivacaine solution following injection into the first intercoccygeal space in foal cadavers and anesthetized foals undergoing laparoscopy.

OBJECTIVE: To determine the relationship between epidural cranial migration and injectate volume of an isotonic solution containing dye in laterally recumbent foal cadavers and evaluate the cranial migration and dermatome analgesia of an epidural dye solution during conditions of laparoscopy in foals. ANIMALS: 19 foal cadavers and 8 pony foals. PROCEDURES: Foal cadavers received an epidural injection of dye solution (0.05, 0.1, 0.15, or 0.2 mL/kg) containing 1.2 mg of new methylene blue (NMB)/mL of saline (0.9% NaCl) solution. Length of the dye column and number of intervertebral spaces cranial and caudal to the injection site were measured. Anesthetized foals received an epidural injection of dye solution (0.2 mL/kg) containing saline solution or 2% mepivacaine. Foals were placed in a 100 head-down position, and pneumoperitoneum was induced. Dermatome analgesia was determined by use of a described electrical stimulus technique. Foals were euthanatized, and length of the dye column was measured. RESULTS: Epidural cranial migration of dye solution in foal cadavers increased with increasing volume injected. No significant difference was found in epidural cranial migration of a dye solution (0.2 mL/kg) between anesthetized foals undergoing conditions of laparoscopy and foal cadavers in lateral recumbency. Further craniad migration of the dye column occurred than indicated by dermatome analgesia. CONCLUSIONS AND CLINICAL RELEVANCE: Epidural cranial migration increases with volume of injectate. On the basis of dermatome analgesia, an epidural injection of 2% mepivacaine (0.2 mL/kg) alone provides analgesia up to at least the caudal thoracic dermatome and could permit caudal laparoscopic surgical procedures in foals.

Anesthesia, Epidural↗
PubMed1989

[DNA synthesis in cells of human cadavers].

Punch biopsies of human cadavers were incubated in vitro with radioactively labeled thymidine (3H-TdR, 14C-TdR) to get information on postmortal cytokinetics. The investigation established that 3H-TdR really was incorporated in DNA by cells of human cadavers. Basal cells of epidermis as well bone marrow cells of cadavers stored at 20 degrees C incorporated 3H-TdR during a storage time of at least 32 hours. Storing the cadavers at 4 degrees C DNA synthesis in epidermic cells was demonstrated for more than 77 hours. While spermatogonia incorporated 3H-TdR during the early postmortal period of 8 hours neither in bowel nor in splenic cells a postmortal DNA synthesis was seen. Systemic investigations were done using punch biopsies taken from the extensor side of the thigh of 45 human cadavers stored at 4 degrees C. The labeling index (mean: 2.53 +/- 0.79%) over a postmortal period of more than 70 hours and the synthesis time determined by the double labeling procedure over a period of at least 30 hours remained essentially unchanged and were comparable with live humans. The generation time decreased with increasing storage time--but not statistically relevant differences, however, were established at the 1% level. As well no statistically relevant differences were established by correlating the kinetic data according to sex, age and site.

Autoradiography↗