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PubMed1979

Restriction endonuclease mapping by crossed contact hybridization: the ribosomal RNA genes of Achlya ambisexualis.

A rapid, convenient and economical method for the hybridization of electrophoretically resolved RNA to DNA restriction fragments immobilized on nitrocellulose filters is described. DNA was digested, electrophoresed on agarose gels in a wide band and transferred to a nitrocellulose filter. The filter was then placed on the surface of a second gel containing radioactively labeled RNA electrophoresed under denaturing conditions in a similar way. The filter and gel were oriented so that the DNA and RNA bands were perpendicular to one another and the RNA was transferred from the gel through the filter under conditions which promote RNA-DNA hybridization. Following washing, the filter was autoradiographed. RNA-DNA sequence relationships could be conveniently determined from the spots produced at regions of intersection of homologous nucleic acids. The two dimensional array formed in this procedure fascilitates the rapid ordering of DNA restriction fragments. An example of its use for this purpose is presented.

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PubMedDate not supplied

Epizootic infection due to Achlya debaryana in a catfish.

Achyla debaryana (Saprolegniales, Oomycetes) is being reported for the first time as a fish pathogen, causing an epizootic of mycosis in a catfish, Mastacembelus armatus, in Nanak Sugar, a huge artificial reservoir and recognized fish production centre in Naini Tal district, Uttar Pradesh, India. The incidence of infection was found to be influenced by the physicochemical characteristics of water. The maximum disease severity (52 and 47.4%) occurred in March with moderate water temperature (22-24 degrees C), high dissolved oxygen content (9.5 mg l-1) and pH 8.6, while the lowest level of infection (3 and 1.8%) was recorded at high temperature (29-32 degrees C) during June.

Animals↗