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Heat acclimatization by a method utilizing microclimate cooling.

A new approach to heat acclimatization has been shown to be feasible during laboratory experimentation. Wearing microclimate suits containing dry ice as the coolant, three groups of men were subjected to a moderate work rate in three different environments for 4 h/d for 8 d. Physiological responses on a subsequent heat tolerance test indicate that the group subjected to an environment of 32.0 degrees C W. B. and 33.5 degrees C D.B. were fully heat acclimatized. The 33/35 degrees C group were also well-acclimatized but developed dangerously high body temperatures during the first 2 d. Only partial acclimatization was achieved by the 31/33 degrees C group. The reason why the men acquire heat acclimatization while wearing the microclimate suits in a hot environment is probably that microclimate cooling does not prevent body temperature from rising--it only prevents it from rising excessively. It should be remembered that only one-third of the body is cooled while the rest shows the normal sweating response.

Acclimatization↗

The effect of altitude pre-acclimatization on acute mountain sickness during reexposure.

BACKGROUND: Acclimatization to high altitude appears to prevent acute mountain sickness (AMS), as evidenced by a decline in AMS symptoms as acclimatization progresses. HYPOTHESIS: We hypothesized that partial retention of acclimatization would attenuate the incidence and/or severity of AMS upon reinduction to altitude. METHODS: To test this hypothesis 6 male lowlanders returned to sea level after the acclimatizing of the 16 d at 4300 m (HA). After 8 d at sea level (PA), they were reexposed to 4300 m in a hypobaric chamber for 30 h (RA). AMS symptom severity was determined by the AMS-cerebral (AMS-C) scores calculated from the daily administration of the Environmental Symptoms Questionnaire during HA and RA. RESULTS: The mean AMS-C scores were reduced from 0.6 on HA day 1 (HA1) to 0.1 during RA (p < 0.05). Four subjects were "sick" (AMS-C > 0.7) during HA1, while only one was "sick" during RA. The % oxyhemoglobin, hemoglobin concentration and hematocrit were higher during RA compared to HA1. CONCLUSIONS: These results suggest that the retention of acclimatization after 8 d at low altitude is sufficient to attenuate AMS upon reinduction to high altitude.

Acclimatization↗

Effect of heat acclimatization on testicular enzymes involved in androgen biosynthesis via the 5-ene pathway.

The metabolism of 3beta-hydroxy-5-ene steroids by testicular homogenates of heat-acclimatized and control mice was investigated in vitro. Acclimatization was achieved by keeping the animals in a hot room (33-35 degrees C, 25-40% R.H.) for 5 weeks. The control animals were kept in a temperate environment (20-22 degrees C, 30-50% R.H.). Some of the heat-acclimatized animals were supplied with additional water in a trough placed inside each cage (HAII mice). This source of water was used by the mice mainly for body cooling. A pronounced decrease in body weight and testis weight, and a smaller decrease in the weight of seminal vesicles which was associated with atrophy of the seminiferous tubules and hyperplasia of the Leydig cells was characteristic of heat-acclimatized mice with only drinking water available (HAI), but not of HAII mice. Although body cooling abolished the adverse physiological response described, it did not prevent specific changes in enzyme activity associated with androgen production. There was increased activity of 3beta-hydroxysteroid dehydrogenase and isomerase, and of 5-ene-17beta-hydroxysteroid dehydrogenase. These data offer partial explanation for the lower peripheral blood testosterone level observed in some species of heat-acclimatized mammals.

17-alpha-Hydroxypregnenolone↗

Transcription factor pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: identification of two pit-1 genes in the carp.

A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer-acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5'-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer-acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat.

Amino Acid Sequence↗

[Identification of differentially expressed genes of acute hypoxia-treated HepG2 cells and hypoxia-acclimatized HepG2 cells].

To provide necessary information for further understanding of molecular mechanism of hypoxia acclimatization, the differentially expressed genes of HepG2 cells exposed to normoxia, acute hypoxia-treated cells which were exposed to 1% oxygen for 48 h, and hypoxia-acclimatized HepG2 cells which were cultured for 6 circles of alternate low oxygen (1% oxygen for 24 h) and normal oxygen (21% oxygen for 24 h), were identified respectively by combining the suppression subtractive hybridization (SSH) and cDNA microarray. Thirty-seven genes were expressed differentially in cells exposed to 1% oxygen for 48 h compared with those in cells exposed to normoxia. The expression of all these 37 genes was down-regulated, including the genes participating in cell cycle, cell response to stimulus, and cell signal transduction, and cell cytoskeleton formation, the genes associated with transcription and cell metabolism, 4 expressed sequence tags (ESTs), and 12 genes of which the functions are not known. There is a novel gene sequence, which has not been found in existing databases. There were only 6 genes differentially expressed in the hypoxia-acclimatized cells compared with cells exposed to normoxia, including two mitochondrion genes, metalloprotease-1 gene, ferritin gene, thymosin beta-4 and TPT1 genes. The expressions of mitochondrion ND4, ferritin, thymosin beta-4 and TPT1 were up-regulated, while the expressions of mitochondrion ND1 gene and metalloproease-1 gene were down-regulated. Cell tolerance to hypoxia increased after the cells were hypoxia-acclimatized. The different gene expression patterns of the acute hypoxia-treated cells and the hypoxia-acclimatized cells may be related to the increased tolerance of the cells to hypoxia.

Adaptation, Physiological↗

One-step acclimatization of plantlets using a mist reactor.

A mist reactor was used to grow and acclimatize carnation plants in vitro without using ex vitro acclimatization techniques. The acclimatization protocol in the reactor consisted of altering the mist-on period during the course of the culture period and a stepwise reduction in the relative humidity surrounding the plants from 98% to 70% relative humidity (RH) during the final week of in vitro growth. After transfer and further growth in a greenhouse for 5 weeks, survival was 91% for plants grown in reactors, 81% from vented boxes, and 50% from unvented boxes. Ex vitro survival directly correlated with increased in vitro rooting and decreased hyperhydration. In vitro rooting also correlated with high-quality plants, but did not significantly correlate with low hyperhydration, as normal plants often lacked roots. After 5 weeks in the greenhouse, the quantity of mid- and high-quality plants obtained from reactors and ventilated boxes was similar. Conditions in the mist reactor can be manipulated to produce plants that are readily acclimatized and are equal or better in quality and yield than plants produced using conventional methods.

Acclimatization↗

Plasma arginine vasopressin, atrial natriuretic peptide and brain natriuretic peptide responses to long-term field training in the heat: effects of fluid ingestion and acclimatization.

The maintenance of blood volume during exercise, especially in a hot environment, is of major importance for continued performance. In order to investigate the relationships between exercise, type and amount of fluid intake and the degree of acclimatization to heat stress and on responses of arginine vasopressin (AVP), atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), we studied 24 soldiers during and after jogging/walking exercise both before and after acclimatization to field training at [mean (SE)] 40 (0.7) degrees C and 32 (3)% relative humidity. The running exercise was carried out under three conditions, i.e., (1) without any fluid intake, (2) with intake of water or (3) with intake of a dextrose/electrolyte solution. Venous blood samples were drawn before exercise, at the end of exercise and at 15 min and 60 min afterwards. Acclimatization resulted in significant losses of body mass, total body water, plasma volume, ANP and increases in plasma osmolality, packed cell volume and AVP at rest but without any significant changes in BNP. During exercise with no fluid intake, there was a significant rise in plasma osmolality, Na+ and AVP, but no significant alterations in plasma ANP and BNP were observed. When subjects ingested water or dextrose/electrolyte solution during exercise, ANP rose by 234% and 431% respectively and BNP rose by 398% and 583% respectively without any significant increase in AVP. The results suggest that, during acclimatization, the subjects became slightly dehydrated. Alterations in response to changes in body water status appear to be greater for AVP than ANP or BNP at rest. During exercise in the heat ANP and BNP may play complementary roles.

Acclimatization↗

Very mild exposure to hypoxia for 8 h can induce ventilatory acclimatization in humans.

Ventilatory acclimatization to altitude is associated with a progressive increase in ventilation, a progressive decrease in end-tidal PCO2 and a progressive increase in the acute ventilatory sensitivity to hypoxia. Ventilatory acclimatization has been observed with mild exposure to hypoxia when the duration of exposure has been of some length (e.g. days), and with shorter duration exposures (e.g. 8 h) when the degree of hypoxia has been more severe. The purpose of this study was to determine whether short-duration exposures to very mild hypoxia, such as are commonly associated with the reduction in cabin pressure during commercial airline flight, can also induce some degree of ventilatory acclimatization. Twelve subjects were exposed in a chamber to both 8 h mild hypoxia (inspired PO2 127 mmHg) and 8 h air-breathing as a control. Exposures were on different days in random order. Following the hypoxic exposure, there was a significant reduction in end-tidal PCO2 during air breathing (from 39.2+/-1.8 to 38.11+/-1.5 mmHg, mean +/- SD, P<0.05), and a significant increase in ventilatory sensitivity to hypoxia (from 0.84+/-0.54 l/min/% to 1.13+/-0.66 l/min/%, P<0.05). We conclude that shortterm exposures to very mild hypoxia do induce significant acclimatization within the respiratory control system.

Acclimatization↗

Role of adiposity hormones in the mouse during fasting and winter-acclimatization.

The influence of fasting and winter-acclimatization (cold and short-day acclimatization) on mouse plasma leptin, ghrelin, growth hormone (GH) and melatonin concentrations was determined from blood samples taken at mid-day and midnight. A 16-h fast decreased the plasma leptin but almost doubled the plasma ghrelin concentrations which contribute to energy saving, appetite stimulation and, in the case of leptin, to inhibition of reproduction. Winter-acclimatization did not affect plasma ghrelin concentrations, whereas leptin decreased to the same level as in fasting. The low leptin concentrations possibly enable an increased caloric intake for the purpose of thermogenesis. Fasting and winter-acclimatization seemed to abolish the diurnal leptin rhythm, but had no effect on that of ghrelin. Plasma melatonin concentration correlated negatively with ghrelin, suggesting a possible role for melatonin in the regulation of ghrelin concentration. SNS-activity and insulin appear to be the main regulators of leptin plasma concentration in the mouse, rather than melatonin as in some seasonal mammals. Interestingly, endogenous ghrelin did not stimulate GH secretion, which is a well-documented reaction to exogenous ghrelin injections.

Acclimatization↗

Breathing in thin air: acclimatization to altitude in ducks.

We measured ventilation (VI) and arterial blood gases in Pekin ducks during acclimatization to 3800 m altitude for 1-90 days. Four experimental series were conducted over 4 years using both natural altitude and a hypobaric chamber. PaCO2 decreased to 3.5 Torr, relative to the value measured during acute hypoxia after 1 day and remained at this level for up to 90 days. However, PaO2 did not increase. Arterial pH showed an unexpected metabolic alkalosis during the first hours at altitude but after 3 days, a metabolic acidosis partially compensated the respiratory alkalosis and pHa was constant thereafter. When normoxia was restored after hypoxia, PaCO2 was 5.5 Torr less than the original normoxic control value, but PaO2 was not increased. VI showed variable changes during acclimatization but if metabolic rate was constant in our study, as reported by others, then effective parabronchial V(VP) increased during acclimatization. Increased VP tends to restore PaO2 toward normoxic levels and decreases adverse effects of gas exchange limitation, which apparently increased during acclimatization in ducks.

Acclimatization↗

Acclimatization, preconditioning and heat shock proteins.

During prior acclimatization, mineworkers acquire tolerance against the adverse effects of working in a warm environment. In this article, the possible involvement of heat shock proteins as mediators of acclimatization is proposed. Acclimatization is compared with preconditioning. Preconditioning of isolated cells or organs by prior exposure to a temperature higher than normal or exposure to an ischaemic insult endow tolerance on them when later confronted with a severe ischaemic stress. This tolerance is possibly mediated by heat shock proteins induced by the heating or ischaemic preconditioning episode. Functioning of the induced heat shock proteins may therefore underlie the protective mechanisms in both acclimatization and preconditioning.

Acclimatization↗

Effect of sildenafil and acclimatization on cerebral oxygenation at altitude.

Phosphodiesterase-5 inhibitors decrease hypoxic pulmonary vasoconstriction under hypobaric hypoxia, but are not known to affect cerebral blood flow or oxygenation. The present study was designed to evaluate the effect of sildenafil on cerebral haemodynamics during acute exposure to altitude and after acclimatization. Ten subjects were studied 1 and 3 days after rapid ascent to 3480 m before and for two consecutive hours after taking sildenafil (50 mg). Before acclimatization, HR (heart rate) rose at 1 h (76.3+/-1.0 beats/min compared with 72.5+/-1.5 beats/min at baseline; P<0.05) and had returned to baseline at 2 h (71.3+/-1.1 beats/min; P>0.05). Mean BP (blood pressure) fell from 96.0+/-2.0 mmHg at baseline to 91.7+/-2.5 (P<0.001) at 1 h and 89.8+/-1.8 mmHg (P<0.0001) at 2 h, whereas SaO2 (arterial oxygen saturation) increased from 83.9+/-0.5% at baseline to 85.3+/-0.4% (P<0.0001) at 1 h and 85.0+/-0.5% (P<0.01) at 2 h. MCAV [MCA (middle cerebral artery) velocity] and PETCO2 (end-tidal partial pressure of CO2) were unchanged, but rSO2 (regional cerebral oxygen saturation) rose progressively at 1 h (62.7+/-0.8%; P<0.05) and 2 h (65.3+/-0.9%; P<0.0001) compared with baseline (59.3+/-1.3%). After 3 days of acclimatization, resting rSO2 and RMCA (MCA resistance) increased and oxygen delivery fell. Changes in HR and mean BP after sildenafil were similar to day 1, but SaO2 did not change. However, rSO2 increased [61.7+/-0.9% at baseline to 65.0+/-1.0% (P<0.0001) at 1 h and 64.0+/-0.9% (P<0.001) at 2 h], despite a reduction in MCAV [65.3+/-1.8 cm/s at baseline to 61.3+/-1.5 cm/s (P<0.01) at 1 h and 60.9+/-1.7 cm/s (P<0.0001) at 2 h] and PETCO2 [4.1+/-0.05 kPa at baseline to 4.0+/-0.04 kPa at 2 h (P<0.01)]. These observations suggest that sildenafil improves cerebral oxygenation at altitude. Whereas the early changes before acclimatization may be largely pulmonary in origin, the later observations may be a direct cerebral effect which warrants further study.

Acclimatization↗

A zinc finger protein RHL41 mediates the light acclimatization response in Arabidopsis.

Arabidopsis thaliana plants showed an increased tolerance to high-intensity light when pre-exposed to medium-intensity light. This response, known as light acclimatization, depended on the quantity of light, the period of irradiation, and the quality of light. Among characterized acclimatization-induced cDNA clones, we identified a zinc finger protein rhl41 (responsive to high light) gene, that was rapidly up-regulated in proportion to the time of irradiation and the light intensity. Transgenic Arabidopsis plants over-expressing the rhl41 gene showed an increased tolerance to high-intensity light, and also morphological changes of thicker and dark green leaves. Interestingly, the palisade parenchyma was highly developed in the leaves of the transgenic plants, which is one of the long-term acclimatization responses in Arabidopsis plants. The anthocyanin content (a light protectant) as well as the chlorophyll content also increased. Antisense transgenic plants exhibited decreased tolerance to high irradiation. We propose that the RHL41 zinc finger protein has a key role in the acclimatization response to changes in light intensity.

Acclimatization↗

Acclimatization of the fowl to intermittent acute heat stress.

The acclimatization of poultry to a hot, dry climate was assessed by studying changes in rectal temperature (Tr) during regular daily exposure to an ambient temperature of 38 degrees C and 26% RH. Laying hens of two strains, young chicks, growing broilers and broiler breeders were able to acclimatise, in varying degrees, to these conditions. Acclimatization was characterised by a progressive reduction in the rate of increase in Tr over the period of exposure and the ability to survive conditions that initially would have been fatal. Laying hens could acclimatise if prevented from displaying their normal postural responses to heat stress. Acclimatization was accompanied by a decrease in oxygen consumption when measured at either the acclimatization temperature or at a thermoneutral temperature.

Acclimatization↗

Effect of seasonal acclimatization on the expression of the carp transcription factor Pit-1.

We isolated a clone comprising four exons of the carp Pit-1 gene. Using synthetic oligonucleotide probes derived from the carp Pit-1 sequence Pit-1 expression was assessed by in situ hybridization in pituitary sections from summer- and winter-acclimatized carp. Semiquantitative analyses of the hybridization signals revealed a significant higher Pit-1 expression in the proximal pars distalis (PPD) and pars intermedia (PI) of the pituitary glands from summer-acclimatized carp, compared to the winter-acclimatized fish. In both adaptive states, relative to the PPD and PI, only a basal Pit-1 expression was detected in the rostral pars distalis. Thus, during seasonal acclimatization of an eurythermal fish, Pit-1 seems to be involved in the mechanisms that underlie the compensatory response.

Acclimatization↗

Exercise responses after altitude acclimatization are retained during reintroduction to altitude.

Following 2 to 3 wk of altitude acclimatization, ventilation is increased and heart rate (HR), plasma volume (PV), and lactate accumulation ([La]) are decreased during submaximal exercise. The objective of this study was to determine whether some degree of these exercise responses associated with acclimatization would be retained upon reintroduction to altitude (RA) after 8 d at sea level (SL). Six male lowlanders (X +/- SE; 31 +/- 2 yr, 82.4 +/- 4.6 kg) exercised to exhaustion at the same relative percentages of peak oxygen uptake (VO2peak) at SL, on acute altitude (AA) exposure, after a 16-d chronic altitude (CA) exposure on Pikes Peak (4,300 m), and during a 3- to 4-h RA in a hypobaric chamber (4,300 m; 446 mm Hg) after 8 d at SL. The submaximal exercise to exhaustion time (min) was the same at SL (66.0 +/- 1.6), AA (67.7 +/- 7.3), CA (79.9 +/- 6.2), and RA (67.9 +/- 1.9). At 75% VO2peak: (1) arterial oxygen saturation (SaO2) increased from AA to CA (67.0 +/- 1.5 vs 78.5 +/- 1.8%; P < 0.05) and remained increased at RA (77.0 +/- 2.0%); (2) HR decreased from SL to CA (171 +/- 6 vs 152 +/- 9 beats x min-1; P < 0.05) and remained decreased at RA (157 +/- 5 beats x min-1); (3) calculated PV decreased 6.9 +/- 10.0% at AA, 21.3 +/- 11.1% at CA, and 16.7 +/- 5.4% at RA from SL baseline values, and (4) [La] decreased from AA to CA (5.1 +/- 0.9 vs 1.9 +/- 0.4 mmol x L-1; P < 0.05) and remained decreased at RA (2.6 +/- 0.6 mmol x L-1). Upon RA after 8 d at SL, the acclimatization responses were retained 92 +/- 9% for SaO2, 74 +/- 8% for PV, and 58 +/- 3% for [La] at 75% VO2peak. In conclusion, although submaximal exercise to exhaustion time is not improved upon reintroduction to altitude after 8 d at sea level, retention of beneficial exercise responses associated with altitude acclimatization is likely in individuals whose work, athletic competition, or recreation schedules involve intermittent sojourns to high elevations.

Acclimatization↗

Cardiovascular and metabolic responses to noradrenaline in man, before and after acclimatization to cold in Antarctica.

1. Four men of European descent were infused with noradrenaline at rates of 0.038, 0.075, 0.150 and 0.300 mug/kg.min in Australia, and again after 29 weeks' residence at Mawson, Antarctica. A concurrent study of their responses to whole-body cooling showed that they acclimatized to cold in Antarctica.2. Blood pressure rose and heart rate fell in proportion to the dose of noradrenaline infused. The response was much less after than before acclimatization in three of the four subjects. Subjective effects of the drug decreased in proportion to the decrease in the pressor effect.3. Finger temperature fell in proportion to the dose infused, in three subjects. The response was unchanged or increased after acclimatization.4. Oxygen consumption was initially unaffected by noradrenaline, but after acclimatization it apparently increased in proportion to the dose infused. The increase in pulmonary ventilation during infusion was slightly greater after acclimatization.

Acclimatization↗

Increased beta-adrenergic receptors in brown fat of winter-acclimatized Alaskan voles.

To assess a possible mechanism for the enhanced thermogenesis of cold-acclimated and winter-acclimatized red-backed voles (Clethrionomys rutilus), beta-adrenergic receptors of brown fat were characterized by specific binding of (-)-[3H]-dihydroalprenolol [( 3H]DHA) to isolated brown fat membranes from 23 degrees C-acclimated controls, cold-acclimated (5 wk or 5 mo at 5 degrees C), wild summer, and winter-acclimatized voles. Scatchard analysis to determine the equilibrium dissociation constant (Kd) and the maximum number of binding sites (Bmax) for control brown fat membranes gave a Kd of 4.45 nM [3H]DHA and Bmax of 249 fmol [3H]DHA bound per milligram of protein. beta-Adrenergic agonists competed for specific binding sites with an order of potency typical of the beta 1 subtype of adrenergic receptors: (-)-isoproterenol greater than (-)-norepinephrine greater than or equal to (-)-epinephrine. After cold acclimation for 5 wk or 5 mo, the Kd and Bmax for adrenergic binding sites were similar to those of controls. Brown fat mass was 1.5 times greater than that of controls after 5 wk cold acclimation but similar to controls after 5 mo cold acclimation. Winter voles had 1.7 times higher Bmax and 1.6 times more brown fat than summer voles. Thus seasonal acclimatization to winter in red-backed voles appears to involve an increase in beta-adrenergic receptors in brown fat, but cold acclimation does not. The results suggest quantitative and possibly qualitative differences in neural and hormonal stimulation of brown fat between cold acclimation and winter acclimatization in voles.

Acclimatization↗