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Effects on heat stress of a flame-retardant ensemble for aluminum smelters.

A common belief is that a flame-retardant clothing ensemble will increase the level of heat stress over ordinary cotton work clothes. This is supported by bench tests on fabrics that indicate higher insulation and vapor resistance values for flame-retardant clothing. This research compared a flame-retardant clothing ensemble for an aluminum smelter (Zirpo wool shirt and FR8 denim pants) with typical cotton work clothing. Four young men walked on a treadmill at two work levels inside a climatic chamber under controlled conditions of heat stress. During each test, heart rate, core temperature, and skin temperatures were continuously monitored and recorded every 5 min. After a physiological steady state was achieved, temperature and humidity were slowly increased to maintain a relative humidity of 50%. The critical condition was the time when thermal regulatory control was lost (called the inflection point, marked by a steady increase in core temperature). The climatic conditions at the inflection point were used to assign a critical wet-bulb globe temperature (WBGT). A three-way analysis of variance examined the effects on critical WBGT of clothing, work level, subjects, and the interaction between clothing and work level. There were no significant findings. Therefore, there should be no difference in the level of heat stress between the two clothing ensembles under the same environmental and work conditions.

Adult↗

External and internal exposure to polycyclic aromatic hydrocarbons (PAH) among workers in the production of fire-proof materials - proposal of a biological monitoring guidance value.

In 1999, we introduced the German polycyclic aromatic hydrocarbons (PAH) study. The study was designed as a nation-wide investigation on workers exposed to PAH. One aim of the study was to establish biological monitoring guidance values (BMGVs) for different branches. Here, we report on the production of fire-proof materials. This branch of industry is typically confronted with high exposure to PAH and with PAH-induced occupational (cancer) diseases. One hundred and thirty-five employees participated in the course of seven sampling dates in four different plants in Germany. External exposure was determined by personal air monitoring of the 16 EPA-PAH. Human biological monitoring was accomplished by the determination of 1-hydroxypyrene and monohydroxyphenanthrenes in post-shift spot urine samples. Concentrations of PAH in the air of the workplaces ranged up to 1102.6microg/m(3). Maximum benzo[a]pyrene concentration was 38.2microg/m(3). The internal PAH exposure of workers was much higher compared with that of the general population. Median concentration for 1-hydroxypyrene was 6.4microg/g creatinine (maximum 279.6, 90th percentile 23.9microg/g creatinine) and for the sum of monohydroxyphenanthrene metabolites 13.3microg/g creatinine (maximum 313.4, 90th percentile 70.8microg/g creatinine). The following BMGVs for the non-smokers of this branch of industry are proposed: for 1-hydroxypyrene 18microg/g creatinine and for the sum of hydroxyphenanthrenes 77microg/g creatinine in urine measured at the end of the shift.

Adult↗

Multiple exposure to arsenic, antimony, and other elements in art glass manufacturing.

Art glass manufacturing is one of the most interesting examples of exposure to complex mixtures. Among the raw materials used are silica sand, borax, carbonates, nitrates of Ca, Na, K, and a great number of compounds that are mainly oxides of As, Sb, Al, Zn, Cr, Ni, Sn, Se, Cd, Mn, Cu, Co, Fe, Nd, Er, Eu, and La. In six art glass factories that use As or Sb as fining agents, the exposure to these elements was investigated in 32 workers by means of environmental and biological monitoring. Analysis was conducted by atomic absorption spectrometry (AAS) and inductively coupled plasma mass spectrometry (ICP-MS). The results confirmed that As, which is the main carcinogen in glass production, reaches high air concentrations and is generally above the American Conference of Governmental Industrial Hygienists (ACGIH) threshold limit value-time-weighted average (TLV-TWA) of 10 micrograms/m3. When partly substituted by antimonial compounds, As air concentrations dropped dramatically, while the air levels of Sb (which is considered less toxic but is classified as a class 2B carcinogen by IARC) were relatively low and below the ACGIH TLV-TWA of 500 micrograms/m3. Exposure to As and Sb also differed in the three types of jobs investigated: As was high in oven chargers, Sb was higher in batch mixers, and both elements were low in makers-formers. Eleven to 18 elements were detected: arsenic, Al, Ba, Sb, Pb, and Zn were the elements most frequently measured (in tens, and in some cases hundreds, of micrograms/m3), followed by B, Li, Mn, Se, Sn, Sr, Ce, La, and Nd (in micrograms or in some cases tens of micrograms/m3. The results of biological monitoring for As, Sb, and other elements were in agreement with environmental monitoring data. We concluded that multiple detection of elements is a useful tool (or the evaluation of exposure to complex mixtures such as those used in the art glass industry and that such detection also allows a more accurate evaluation of related epidemiological data.

Antimony↗

Evaluation of the size and type of free particulates collected from unused asbestos-containing brake components as related to potential for respirability.

BACKGROUND: Chrysotile asbestos has found multiple applications in the production of friction products. At one point it comprised 40-50% of the composition of brake linings thus generating the potential for the development of asbestos related diseases in millions of workers involved in vehicle repairs. While some attention has been given to the health status of workers involved in the handling of worn components, little has been given to the potential for exposure during the handling and fitting of new (unused) components as replacement parts. METHODS: Unused brake linings or brake shoes with attached linings from four different sources were gently rinsed with prefiltered water that was then collected on filters for analysis by Analytical Transmission Electron Microscope. RESULTS: Large numbers of chrysotile asbestos containing structures, the majority of respirable size, were present in each sample. CONCLUSIONS: We conclude that any manipulation of new asbestos containing brake components would be expected to yield free dust containing chrysotile asbestos of respirable size. That the vast majority of these fibers and particulates would not be counted as regulated fibers rendering assessment of potential exposures in the work place, based on data of Permissible Exposure Limits or Regulated Fibers, questionable.

Air Pollutants, Occupational↗

Chemical and physical characteristics of cellulose insulation particulates, and evaluation of potential acute pulmonary toxicity.

BACKGROUND: During installation of cellulose insulation (CI) in new and older houses, significant quantities of airborne material are generated. This study characterized the chemical and physical properties, and potential acute pulmonary toxicity of CI. METHODS: CI from four manufacturers was analyzed for inorganic additives and trace element impurities. Aerosols were generated and size fractionated. The number and size of fibrous and nonfibrous particles in the respirable fractions were determined. Respirable CI particulates were intratracheally instilled in rats (5 mg/kg) to evaluate potential pulmonary toxicity. RESULTS: CI samples were similar in composition with small differences due primarily to fire retardants. Less than 0.1% of CI was respirable and contained few fibers. Acute exposure to CI caused transient inflammation in the lungs and increased 4-hydroxyproline. Microscopic evaluation revealed a minimal to mild, non-progressing granulomatous pneumonitis. CONCLUSIONS: Low concentrations of respirable particles were found in CI aerosols. Particles consisted primarily of fire retardants with few fibers, and caused mild pulmonary toxicity in rats.

Administration, Inhalation↗

Reducing attenuation in exposure-response relationships by exposure modeling and grouping: the relationship between wood dust exposure and lung function.

BACKGROUND: In a study of wood dust exposure and lung function, we tested the effect on the exposure-response relationship of six different exposure metrics using the mean measured exposure of each subject versus the mean exposure based on various methods of grouping subjects, including job-based groups and groups based on an empirical model of the determinants of exposure. METHODS: Multiple linear regression was used to examine the association between wood dust concentration and forced expiratory volume in 1s (FEV(1)), adjusting for age, sex, height, race, pediatric asthma, and smoking. RESULTS: Stronger point estimates of the exposure-response relationships were observed when exposures were based on increasing levels of aggregation, allowing the relationships to be found statistically significant in four of the six metrics. The strongest point estimates were found when exposures were based on the determinants of exposure model. CONCLUSIONS: Determinants of exposure modeling offers the potential for improvement in risk estimation equivalent to or beyond that from job-based exposure grouping.

Adolescent↗

Re: Evaluation of the size and type of free particulates collected from unused asbestos-containing brake components as related to potential for respirability.

BACKGROUND: In Atkinson et al. 2004 rinsates of unused brake components were analyzed by transmission electron microscopy (TEM) for the presence of asbestos fibers. RESULTS: We do not believe that the findings of Atkinson et al. are informative and could have been predicted based on the study design and the fact that one would expect to find measurable TEM asbestos fibers on an unused brake component. We also find that the paper did not provide a full or even partial discussion of the published literature with respect to industrial hygiene or epidemiology data. CONCLUSION: The findings of Atkinson et al. do not, in our view, "further raise concerns" about historical asbestos exposures experienced by automotive mechanics because of the vast amount of published literature to the contrary.

Air Pollutants, Occupational↗

Respiratory peripheral chemosensory irritation, acute and repeated exposure toxicity studies with aerosols of triethylene glycol.

The potential for adverse effects from exposure to respirable aerosols of triethylene glycol (TEG: CAS Number 112-27-6) was investigated by a peripheral chemosensory irritation study, and by acute and repeated exposure toxicity studies. The sensory irritation study, conducted with male Swiss Webster mice, showed an exposure concentration-related depression of breathing rate that allowed the calculation of an RD50 of 5140 mg m(-3). In an acute study male and female Sprague Dawley rats were exposed whole body to aerosols of TEG up to 6730 mg m(-3) for 4 h. No mortalities occurred at this high concentration, but unexplained mortality occurred in female rats at 5230 mg m(-3) at 2-3 days postexposure. Two repeats of the 5230 mg m(-3) exposure did not cause mortality. Signs at 6730 and 5230 mg m(-3) were limited to those of irritancy. For a 9 day repeated exposure study rats were exposed whole body to 0, 494, 2011 and 4824 mg m(-3) TEG aerosols for 6 h day(-1). Mortalities occurred at 4824 mg m(-3) between exposure days 2 and 5. Nonspecific indications of toxicity at 2011 mg m(-3) were signs of irritation, decreased body weight and increased food and water consumption; evidence of hepatic dysfunction was indicated by increased serum alkaline phosphatase and alanine aminotransferase activities, but liver histology was normal. Fluid imbalance was suggested by increases in water consumption, blood urea nitrogen, relative kidney weight and urine volume, with decreased urine osmolality, pH and N-acetyl-beta-D-glucosaminidase activity. At 494 mg m(-3) there were minimal signs of irritation, increased water consumption and slightly increased alkaline phosphatase; histology of the kidney was normal. Thus, in this 9 day repeated aerosol whole body exposure study a No-Observed-Effect-Level (NOEL) could not be established. Since preening of the fur at these high aerosol concentrations exposures might have led to a confounding factor from the resultant oral intake, another 9 day repeated aerosol study was conducted, but by nose-only exposure of rats for 6 h day(-1) to TEG aerosol concentrations of 0, 102, 517 and 1036 mg m(-3). In this study there were no clinical signs, no effects on food and water consumption, and no biochemical or histological evidence of hepatorenal dysfunction. By the end of the exposure period, male and female rats of the 1036 mg m(-3) group had body weights lower than those of the controls, but not with statistical significance. Since there were no statistically significant effects on any monitors, 1036 mg m(-3) is considered to be a threshold for toxicity by nose-only exposure to TEG aerosol. The findings indicate that exposure to a respirable aerosol is not acutely harmful, but may cause sensory irritant effects. Repeated exposure to high concentrations of TEG aerosols may be harmful, particularly if there are contributions from additional routes of exposure.

Administration, Inhalation↗

Reduced bone stress as predicted by composite beam theory correlates with cortical bone loss following cemented total hip arthroplasty.

Clinical and experimental evidence suggest that periprosthetic bone loss following total hip arthroplasty is caused in part by stress-shielding. Changes in bone stress in the proximal femur following implantation can be estimated with use of composite beam theory. We hypothesized that the degree of stress-shielding predicted by beam theory correlates with the magnitude of bone loss following cemented total hip arthroplasty. We analyzed cross sections from the proximal femur of 13 patients who had undergone unilateral cemented total hip arthroplasty. A matching implant was inserted contralaterally, and the cross-sectional properties of the implant and bone and the bone density were determined. Bone loss was calculated on the basis of differences between contralateral (control) and ipsilateral (remodeled) sections and correlated to several beam-theory parameters calculated from the control sections: implant rigidity, bone rigidity, ratio of implant to bone rigidity, and predicted decrease in bone stress. All parameters except implant rigidity were significantly correlated with bone loss (p < 0.05). Parameters that included implant and bone properties were more strongly correlated with bone loss than were those based on bone properties alone. The predicted decrease in bone stress explained 50-60% of the variance in bone loss. The data also indicated that patients were not likely to lose substantial amounts of bone unless the reduction in bone stress exceeded a threshold value. Although limited by a small and heterogeneous sample, these results indicate that beam-theory predictions correlate with the degree of femoral resorption and should be investigated further as a means to identify patients at high risk for bone loss.

Arthroplasty, Replacement, Hip↗

Inferences on the mean of zero-inflated lognormal data: the generalized variable approach.

This paper considers the problems of confidence interval estimation and hypothesis testing for the mean of zero-inflated lognormal data. We present an unified novel approach based on the concepts of generalized test variables and generalized pivotal quantities. Simulation results demonstrate that the coverage accuracy of proposed confidence intervals and the type I error control of the proposed exact tests are satisfactory.

Air Pollutants, Occupational↗

In vivo cataract after repeated exposure to ultraviolet radiation.

The purpose of this study was to investigate the effects of repeated close-to-threshold ultraviolet radiation (UVR) doses in the rat lens. Sprague Dawley rats received two UVR exposures (lambda(max) = 300 nm, lambda0.5 = 10 nm) separated by different time intervals. The animals were unilaterally irradiated with 4 kJ m(2-1) UVR in each exposure. The intervals between both exposures were: 6 hr, 1 day, 3 days, 9 days and 30 days. At 1 week after the last exposure both lenses were removed, microphotographs were taken and intensity of forward light scattering was measured. Evaluating the difference between exposed and non-exposed eyes, the forward light scattering in the 6 hr and 1 day interval group was not significantly different. The most intense forward light scattering was found in the group that was allowed 3 days interval between exposures. Thereafter, the intensity of scattering decreased as the time interval between exposures increased. The lowest intensity of forward light scattering was detected in the 30 days interval group. Three days after a UVR exposure, the lens showed the highest sensitivity for a second UVR exposure. One month after the first exposure lenses undergo physiological repair and interactions between exposures seem to decrease.

Animals↗

Detection of Yersinia pestis by pesticin fluorogenic probe-coupled PCR.

The < > assay (PE Applied Biosystems) combines PCR with concomitant release of fluorogenic nucleotides for immediate product detection by fluorometry. Yersinia pestis, the etiological agent of bubonic plague, expresses species-specific genes known to be located on two unique plasmids (9.6-kb pPCP and 100.9-kb pMT). Pesticin (pst) is a unique gene located on pPCP which encodes for a bacteriocin. Using fluorogenic probe coupled PCR as few as three copies of pst targets were detected from total Y. pestis genomic DNA. The pst probe used in this report was positive only for pesticinogenic isolates and did not show complementarity with Yersiniae nor with other bacteria targeted in this study suggesting, that the pst probe is very specific for Y. pestis. Under optimal conditions of Mg(2+)concentration and thermal cycle number, addition of extraneous DNA to respective assay mixtures had no effect on detection.

Bacteriocins↗

Use of partition models to evaluate guidelines for mixtures of volatile organic compounds.

Partition models based on the octanol-air parition coefficients and associated quantitative structure-activity relationships (QSARs) have been developed to describe the triggering of odor response and nasal irritation by common volatile organic compounds (VOCs). This study made use of the QSARs developed by Hau and Connell (1998, Indoor Air 8, 23-33) and Hau et al. (1999, Toxicol. Sci. 47, 93-98) to evaluate risk-based guidelines on the airborne concentrations of common VOCs in the nonindustrial environment. A new concept referred to as the "apparent internal threshold concentration" was developed for evaluating the odor and nasal pungency responses to a typical low-concentration VOC mixture described by Otto et al. (1990, Neurotoxicol. Teratol. 12, 649-652). The assessment indicated that odor can be detected at a total VOC concentration of about 3 mg/m(3), consistent with the findings of Molhave et al. (1991, Atmos. Environ. 25, 1283-1293). Nasal pungency, according to our assessment, should not ocur at a total concentration of 25 mg/m(3), which is apparently in conflict with the findings of Molhave (1986, ASHRAE Trans. 92(1A), 306-316). It can be inferred from this investigation that pure nasal pungency without the influence of odor is unlikely to result from exposure to low-concentration VOC mixtures typically found in the nonindustrial environment.

Air Pollutants↗

A study of the biological partitioning behavior of n-alkanes and n-alkanols in causing anesthetic effects.

n-Alkanes and n-alkanols are two groups of common volatile organic compounds (VOCs) having potential anesthetic effects on workers and building occupants. A partition model based on the octanol-air partition coefficient was developed in this investigation to describe the biological partitioning of n-alkanes and n-alkanols in causing general anesthesia. Data on anesthetic potency (minimum alveolar concentration, MAC) for the test groups in rats were found to fit the model. The slight difference between the n-alkanes and n-alkanols in testing the model could be largely eliminated by correcting for the potential partial pressure gradients of the long-chain alkanes across the blood-brain barrier. The corrected MAC data for the two test groups fit well onto one common activity-partition regression line. This suggests that n-alkanes and n-alkanols may share a common biophase or mechanistic pathway for anesthesia. The present findings may provide some useful insight into setting anesthesia-based health standards for VOC mixtures.

Air Pollutants↗

Biological monitoring of occupational exposure to methyl ethyl ketone in Japanese workers.

The relationship between occupational exposure to methyl ethyl ketone (MEK) and its concentration in urine and blood was studied in a group of 72 workers in a printing factory. Personal exposure monitoring was carried out with passive samplers during the workshifts. The time weighted average (TWA) concentration of MEK ranged from 1.3 to 223.7 ppm, with a mean concentration of 47.6 ppm. In addition to MEK, toluene, xylene, isopropyl alcohol, and ethyl acetate were detected as the main contaminants in all samples. At the end of the workshift, urine samples were collected to determine the urinary MEK, hippuric acid (HA), and creatinine, and blood samples were also collected at the same time for determination of MEK. The concentrations of urinary MEK ranged from 0.20 to 8.08 mg/L with a mean of 1.19 mg/L and significantly correlated with TWA concentrations of MEK in the air with a correlation coefficient of 0.889 for uncorrected urine samples. The concentration of MEK in the blood was also significantly correlated with the TWA concentration of MEK with a correlation coefficient of 0.820. From these relationships, MEK concentrations in urine and blood corresponding to the threshold limit value-TWA (200 ppm; ACGIH 1992) were calculated to be 5.1 mg/L and 3.8 mg/L as a biological exposure index (BEI), respectively. Although the BEI for urinary MEK obtained from the present study was higher than that of previous reports and ACGIH's recommendation (2.0 mg/L), the BEI agreed well with a previous study in Japan.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

The time and space machine: continuous measurement of drug-induced behavior patterns in the rat.

Because of the increasing demand for refined techniques to record drug-induced motoric changes, we designed and evaluated a computer monitoring system with continuous measurement of different parameters of rat motor activity. This system is particularly useful for chronic drug studies because it can characterize patterns of behavior and combines the residential and experimental environments, thus enabling automated behavioral measurement without experimenter intervention. Behavioral responses are detected by a capacitance-sensing device that generates bipolar analog voltages representing the location of the rat in its home cage. These voltages are first transduced, then amplified, and finally converted to digital signals in a computer that processes the input using algorithms to define specific responses. The technique pinpoints the exact location of the rat and identifies many kinds of responses simultaneously (e.g., rearing, circling) by tracking the path of movement or setting threshold limits. Some threshold values (representing rearing, gross movements, fine movements) were validated against stereotypy rating scales for amphetamine, apomorphine, and beta-phenylethylamine. Among these drugs, quantitatively distinct response profiles were obtained. The system has wide applications for studies of biological rhythms, sleep, aging, and drug toxicology.

Animals↗

Oscillatory zones and their role in normal and abnormal sheep Purkinje fiber automaticity.

The mechanisms by which low [K(+)](o) induces spontaneous activity was studied in sheep Purkinje fibers. Purkinje strands were superfused in vitro and membrane potentials were recorded by means of a microelectrode technique. The results show that low [K(+)](o) increases the slope and amplitude of early diastolic depolarization, sharpens the transition between early and late diastolic depolarizations, induces an after-potential and large pre-potentials through a negative shift of an oscillatory zone. Pre-potentials occur progressively sooner during diastole and merge with the after-potential to induce uninterrupted spontaneous discharge. During recovery, when the rate slows, after- and pre-potentials separate once more, the slower discharge decreasing the after-potentials but not the pre-potentials. Low [K(+)](o) has little effect on the plateau, but markedly slows phase 3 repolarization and may altogether prevent it. At depolarized levels, voltage oscillations, slow responses, sinusoidal fluctuations or quiescence may be present depending on voltage. During the recovery, a train of either sub-threshold oscillations or spontaneous action potentials appear towards the end of phase 3 repolarization. The cessation of the action potentials unmasks large sub-threshold oscillations, that occur in the oscillatory zone. Drive, high [Ca(2+)](o) and norepinephrine increase slope and amplitude of early diastolic depolarization as low [K(+)](o) does. In low [K(+)](o), Cs(+) prevents spontaneous discharge at polarized levels, but not the decrease in resting potential nor the onset of slow responses at depolarized levels. Cs(+) blocks the train of oscillations and of action potentials occurring during recovery. We conclude that low [K(+)](o) steepens early diastolic depolarization and increases its amplitude through an after-potential that results from an increased Ca(2+) load; allows the attainment of the threshold through Cs(+)-sensitive voltage oscillations which develop when the oscillatory zone is entered either by diastolic depolarization or by phase 3 repolarization; and causes voltage oscillations also at depolarized levels, but through a Cs(+)-insensitive different mechanism.

Animals↗