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Quantitative data on training new world primates to urinate.

This study assessed the effectiveness of operant conditioning in training three species of captive callitrichid primates (Leontopithecus rosalia, Callithrix geoffroyi, and Saguinus imperator) to urinate on demand. There were three goals to the study: 1) to develop a system for quantitatively assessing positive reinforcement training; 2) to ascertain whether or not positive reinforcement techniques can be used to train callitrichid monkeys to urinate on demand, and if so, how many training sessions are required; and 3) to determine the effect on urination behavior of the trainer entering the cage to collect a urine sample. Positive reinforcement with a continuous reinforcement schedule was used to capture a natural behavior: urination. Training sessions (30 min each) were conducted at dawn thrice weekly during five consecutive phases: habituation, control, training (animals were rewarded for urinating), maintenance (animals had reached a defined training criteria and continued to be rewarded for urinating), and collection (animals were rewarded for urinating, and the trainer entered the cage to collect the sample). The numbers of 30-min training sessions required to train the three monkey species (L. rosalia, C. geoffroyi, and S. imperator) were five, six, and eight, respectively. For the three species, the mean number of urinations per animal was significantly greater during the training, maintenance, and collection phases compared to the control phase. However, the three species differed significantly in the manner in which the rates of urination changed across the five phases. A higher proportion of subjects urinated during the training, maintenance, and collection phases compared to the control phase. Latency to first urination varied significantly across the five phases, with significantly reduced latencies to urinate during the training, maintenance, and collection phases compared to the control phase. The entry of the trainer into the cage to collect the urine sample did not appear to alter urination behavior. We demonstrate that operant conditioning techniques, which typically incur minimal cost, time investment, and disturbance, can be used to increase the quantity of urine samples collected for physiological analysis, the proportion of animals that urinate, and the speed of sample collection.

Animals↗

Effects of reinforcer magnitude on responding under differential-reinforcement-of-low-rate schedules of rats and pigeons.

Experiment I investigated the effects of reinforcer magnitude on differential-reinforcement-of-low-rate (DRL) schedule performance in three phases. In Phase 1, two groups of rats (n = 6 and 5) responded under a DRI. 72-s schedule with reinforcer magnitudes of either 30 or 300 microl of water. After acquisition, the water amounts were reversed for each rat. In Phase 2, the effects of the same reinforcer magnitudes on DRL 18-s schedule performance were examined across conditions. In Phase 3, each rat responded unider a DR1. 18-s schedule in which the water amotnts alternated between 30 and 300 microl daily. Throughout each phase of Experiment 1, the larger reinforcer magnitude resulted in higher response rates and lower reinforcement rates. The peak of the interresponse-time distributions was at a lower value tinder the larger reinforcer magnitude. In Experiment 2, 3 pigeons responded under a DRL 20-s schedule in which reinforcer magnitude (1-s or 6-s access to grain) varied iron session to session. Higher response rates and lower reinforcement rates occurred tinder the longer hopper duration. These results demonstrate that larger reinforcer magnitudes engender less efficient DRL schedule performance in both rats and pigeons, and when reinforcer magnitude was held constant between sessions or was varied daily. The present results are consistent with previous research demonstrating a decrease in efficiency as a function of increased reinforcer magnituide tinder procedures that require a period of time without a specified response. These findings also support the claim that DRI. schedule performance is not governed solely by a timing process.

Animals↗

The use of grafted polynomials and dummy variables in analyzing time series data.

Describes an innovative statistical method for analyzing single-S time series data. The S and data in the paper were selected from a larger study which applied operant conditioning to ambulation problems of severely handicapped patients and used positive verbal reinforcement. The method described is a variation of traditional multiple regression analysis described elsewhere as "grafted polynomials" (Draper & Smith, 1967; Fuller, Note 1). An extensive explanation of the use of dummy variables also is provided.

Conditioning, Operant↗

Biofeedback-assisted EMG reduction and subsequent self-disclosure.

A male E and a female E each conducted a single session of EMG biofeedback training with 24 male and 24 female Ss (N =96) under one of three conditions: verbal reinforcement presented contingently on EMG reduction, yoked noncontingent verbal reinforcement, and no verbal reinforcement. After the biofeedback session, all Ss completed a Journard Self-disclosure Scale. Sex of S and verbal reinforcement conditions did not affect EMG biofeedback performance, but Ss treated by the male E decreased EMG mean and standard deviation more than Ss treated by the female E (p less than .01). Ss who received noncontingent verbal reinforcement reported being least willing to disclose to the E (p less than .05). These results are discussed in terms of the interpersonal influences that may affect biofeedback performance and self-disclosure.

Biofeedback, Psychology↗

Low-intensity magnetic fields alter operant behavior in rats.

The present study demonstrates that operant behavior is affected by a combination of a 60-Hz magnetic field and a magnetostatic field 2.6 X 10(-5) T (about half the geomagnetic field). Rats exposed to this combination for 30 min consistently exhibited changes in the rate and pattern of responding during the differential reinforcement of low rate (DRL) component of a multiple fixed ratio (FR) DRL reinforcement schedule. By contrast, there were no measurable changes following exposure to the static field alone or to the oscillating field alone, even with a 10-fold increase in intensity (5 X 10(-5) to 5 X 10(-4) Trms). A cyclotron resonance mechanism has been suggested as a possible explanation for the observation that weak static magnetic fields modify the response of in vitro brain tissue to low-frequency magnetic fields. The choice of static field intensity Bo and frequency nu in the present study follows from the cyclotron resonance condition nu = (1/2 pi)(q/m)Bo, for singly charged lithium, an element in extensive use in the clinical treatment of affective disorders in humans. The present research is consistent with a cellular cyclotron resonance mechanism and tends to imply a functional dependence of behavior on the geomagnetic field.

Animals↗

The influence of trait anxiety on autonomic response and cognitive performance during an anticipatory anxiety task.

The interaction between emotion and cognition is thought to be intimately involved in the development and maintenance of anxiety disorders. In a set of studies, we investigated whether trait anxiety modulates cognitive performance and autonomic activity during an anticipatory anxiety task. Participants completed a letter-size decision-making task with two alternating 28-32 s background screen color-blocks. One of the colors was associated with the presentation of an aversive noise [unconditioned stimulus (UCS)]. Participants were aware of the background color that would (CTX+) and would not (CTX-) be paired with the UCS but did not know when or how often the UCS would be presented. Two experiments were conducted. In Experiment 1, the UCS was presented during the decision-making task in the CTX+ color-blocks using a partial reinforcement schedule. Different noises were presented each time to increase unpredictability and prevent habituation. In Experiment 2, the UCS was never presented during the decision-making task. Results suggested that only the paradigm used in Experiment 1 was successful in eliciting anticipatory anxiety. In Experiment 1, continuously measured skin conductance response (SCR) data suggested that anxiety was significantly greater during CTX+ compared to CTX- trials; no SCR differences were found between high and low trait-anxious participants. Results further indicated that high trait-anxious participants responded significantly faster on the decision-making task during CTX+ compared to CTX- trials, whereas low trait-anxious participants displayed the opposite pattern. Our results reveal an interesting dissociation between the effects of individual differences in trait anxiety on autonomic activity and cognitive performance during an anticipatory anxiety task.

Adult↗

Acquisition and extinction of an operant response in differentially reared rats.

Rats reared in either socially isolated or control environments were trained to bar press for food on a variable interval schedule of reinforcement, beginning at 125 days of age for 37 consecutive days. Following this phase the subjects were tested for response persistence during an extinction test. Next, the subjects were compared on measures of spontaneous recovery and the rate of response reacquisition when the reinforcement contingencies were once again reinstated. Though no differences were discerned between the groups on measures of acquisition, maintenance, and reacquisition, isolated rats emitted many more responses than the controls during extinction testing.

Animals↗

The effects of compounds related to gamma-aminobutyrate and benzodiazepine receptors on behavioural responses to anxiogenic stimuli in the rat: extinction and successive discrimination.

In a first set of experiments rats were trained to run in a straight alley for food reward on a continuous reinforcement schedule and the running response was then extinguished. On the last 2 days of training and daily throughout extinction different groups of animals were injected IP with saline, 5 mg/kg chlordiazepoxide, 0.75 mg/kg picrotoxin, chlordiazepoxide + picrotoxin, chlordiazepoxide + 1.5 mg/kg bicuculline, 0.00125 or 0.25 mg/kg muscimol, 1 mg/kg baclofen, chlordiazepoxide + baclofen, or 0.00125 mg/kg muscimol + baclofen. Chlordiazepoxide increased resistance to extinction, a well-known anxiolytic effect. This effect was blocked by both picrotoxin and bicuculline. Picrotoxin on its own reduced resistance to extinction (an anxiogenic-like effect). Whether given alone or in combination with other drugs, muscimol and baclofen had no effect. In a second set of experiments rats were trained in a successive operant discrimination (signalled by a flashing or steady light) between components in which sucrose reward was available on a variable-interval schedule for barpressing and components in which no reward was given. Chlordiazepoxide at 10 mg/kg increased responding in both rewarded and nonrewarded components, but more in the latter than could be accounted for by change in the former. This effect is as expected with an anxiolytic drug. It was not altered by administration of bicuculline at 1.5 or 1.75 mg/kg; at 2 mg/kg bicuculline acted synergistically with chlordiazepoxide. Picrotoxin (1 and 1.5 mg/kg) also acted synergistically with chlordiazepoxide, enhancing the latter's rate-increasing effects, but only during rewarded components. Neither muscimol (0.00125 and 0.25 mg/kg) nor baclofen (0.01 mg/kg) affected response rates, whether given alone or in combination. However, baclofen in a dose of 1 mg/kg, provided it was given to rats also injected with muscimol (0.00125 or 0.25 mg/kg) at other times, significantly reduced responding during nonrewarded components (an apparently anxiogenic effect). The results of the two sets of experiments are discussed in relation to the hypothesis that anxiolytic drugs affect behaviour by increasing GABAergic inhibition.

Animals↗

Two tests in rats for antianxiety effect of clinically anxiety attenuating antidepressants.

The effects of mianserin, trazodone, amoxapine, maprotiline, and doxepin were assessed in punishment and pentylenetetrazol drug discrimination paradigms. These two procedures are used to identify antianxiety activity in rats. In the punishment procedure, misanserin produced an inverse dose-related increase in punished responding. The magnitude of the increase in punished responding with mianserin was comparable to that observed with the anxiolytic, buspirone. This effect was not observed with any of the other antidepressants tested. None of the antidepressants were found to be active in antagonizing the discriminative stimulus effects of pentylenetetrazol. In fact, at high doses there was a suggestion that the antidepressants may generalize to the pentylenetetrazol discriminative stimulus. Therefore, several antidepressants with purported clinical antianxiety activity, were not active in two procedures that detect antianxiety activity in rats.

Animals↗