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Phenotypic and molecular characterization of isolates of Ornithobacterium rhinotracheale from Peru.

Twenty-five isolates of Ornithobacterium rhinotracheale were examined by agar gel precipitation, immunoperoxidase assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blot analysis, and a polymerase chain reaction. All of the isolates were identified as serotype A. Protein profiles of whole cell extracts were similar for all the isolates, and a polypeptide with a molecular weight of 33 kD was a major component, being present in all the isolates. In the main, proteins of 33, 42, 52, and 66 kD were recognized in immunoblots with sera from chickens naturally exposed to O. rhinotracheale. A modified polymerase chain reaction assay identified O. rhinotracheale DNA from all the isolates and tracheal swabs, producing amplicons of 784 bp, and distinguished O. rhinotracheale from bacterial agents causing similar clinical signs.

Animals↗

[Early complications of endoscopic retrograde cholangiopancreatography performed from January 1998 to December 2000 at the Daniel Alcides Carrion National Hospital "ELCALLAO", Peru].

OBJECTIVES: The aim of this study was to determine the procedure characteristics of ERCP, indications and frequency of complications, as well as analyzing the associated risk factors. METHODS: ERCPs carried out from January 1998 to December 2000 in the Gastroenterology Service of Daniel Alcides Carri n Hospital were evaluated retrospectively; 17 risk factors were analyzed. RESULTS: A total of 202 ERCPs were carried out in 182 patients, of which 70.3% were patients aged under 60 years and 66.5% were female patients. The most frequent indication was bile duct stone suspect (59.4%). Therapeutic ERCPs were carried out in 49.5% of total ERCPs, diagnostic ERCPs were 33.2%, and failure ERCPs 17.3%. Post-ERCP complications occurred in 5.9% (12 cases), and the most frequent were: acute pancreatitis 3.4% and hemorrhage 1%. There was no mortality case related with complications. A risk factor related with the procedure: pancreatic cannulation or pancreatic contrast injections reached the highest statistic significance (p= 0.063). Therapeutic or failed ERCPs presented significant higher frequency of complications than diagnostic ERCPs: (p=0.0085 and p=0.0381). CONCLUSIONS: the frequency of early post-ERCP complications is 5.9%, and has a direct relation with therapeutic or failed ERCPs. This marks a difference with regards to diagnostic ERCPs. There was no mortality, and pancreatic cannulation or pancreatic contrast injections would be able to determine the development of complications.

Cholangiopancreatography, Endoscopic Retrograde↗

[Psychiatry in Peru].

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Community Psychiatry↗

[Characteristics of antibiotic prescription during office visits in the Hospital Cayetano Heredia in Lima, Peru].

BACKGROUND: The present descriptive cross-sectional study, assesses the proportion of antibiotic prescription (PPA), and the quality of it (CPA) in the ambulatory consultation of the department of medicine at the Cayetano Heredia Hospital, as an approach to antibiotic use in Peruvian hospitals. METHODS: The study population was conformed by the patients who went to outpatient care from January 8th to February 2nd, 2001; a sample size of 120 patients was determined out of 1449 patients, to assess CPA. Data on antibiotic prescription was collected, revised and qualified by three experienced physicians, using as gold standard the United States Pharmacopeial Drug Information criteria. RESULTS: PPA was 13.53% (CI 95%: 11.77%-15.29%), without significant differences according to age, sex, doctor's offices and turns. The most frequent diagnoses with antibiotic prescription were: urinary tract infection and pharyngoamigdalitis; the antibiotics more frequently prescribed were: ciprofloxacine and cotrimoxazol. Of the antibiotics, 70.00% were prescribed as non commercial name and 70.83% were registered in the National Petitory of Essential Drugs. We found that 81.67% (CI 95%: 79.68%-83.66%) of the prescriptions were inadequate mainly due to: duration (59.20%) and dose (20.00%) of the antibiotic treatment. CONCLUSIONS: PPA obtained is lower than those published in, and CPA is as high as the reported one in other studies. The investigations in this field are still scarce. It is important new research in order to know about prescription, use and adverse reactions of antibiotics. It is necessary to develop a antibiotic policy to improve access and rational use of antibiotic.

Adult↗

Peru.

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Americas↗

Peru CPS.

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Americas↗