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Kala-azar in Ballia district, Uttar Pradesh.

Door to door search during 1991 in 85 villages in Ballia district of Uttar Pradesh revealed 29 sporadic cases of Kala-azar in four villages. Epidemiological investigations indicated indigenous transmission in Phulwaria village (PHC: Dubhar) with 25 cases while the remaining three villages showed four imported cases from the endemic states. No kala-azar cases had been reported in Ballia between 1947 and the present investigation. Phlebotomus arqentipes, the known vector of kala-azar in India, was encountered in 10 PHCs including the four villages having kala-azar cases. Regular vigilance in Ballia and the neighbouring districts in Uttar Pradesh bordering Bihar is suggested in view of indigenous transmission detected in one village with multiple infection in families. The presence of high vector density, ambient environmental factors and absence of regular residual insecticidal spray warrant constant surveillance in Kala-Azar prone areas in Uttar Pradesh.

Adolescent↗

Entomological observations on dengue vector mosquitoes following a suspected outbreak of dengue in certain parts of Nagaland with a note on their susceptibility to insecticides.

Three species of Aedes viz., Aedes albopictus, Aedes aegypti and Aedes annandalei were detected from different breeding sources in and around human habitats during entomological study conducted following an outbreak suspected to be of dengue (which occurred during, 1994) in parts of Medziphema PHC area of Nagaland in two different points of time ie., in the year, 1994 and, 2000. The potential dengue vector, Aedes albopictus showed high preponderance by breeding in all types of containers searched with high Breteau Index (BI) value of 85.0 and 72.72 recorded in, 1994 and, 2000 respectively whereas the BI value for other potential vector, Aedes aegypti was recorded low (4.9) in the year, 1994 with a substantial increase (31.81) in, 2000. The change in ecosystem along with the process of urbanization has facilitated the growth of these dengue vector mosquitoes in the area of investigation. Adults of both Aedes aegypti and Aedes albopictus were found to be susceptible to DDT, dieldrin and malathion in insecticide bioassay carried out using WHO test kit.

Aedes↗

Susceptibility status of Japanese encephalitis vectors in Kurnool and Mehboobnagar districts of Andhra Pradesh, India.

An entomological survey in Kurnool and Mahboobnagar districts of Andhra Pradesh state, India was undertaken in January, 2002 to find out vector population of Japanese Encephalitis and their susceptibility status to different insecticides. Five Culicine species viz. of Cx. tritaeniorhynchus, Cx. vishnui, Cx. pseudovishnui, Cx. bitaeniorhynchus, Cx. gelidus were captured during the survey. Another suspected vectors Anopheles subpictus and An. hyrcanus were also prevalent in the study villages. The larval population showed a high degree of resistance to Fenthion and Temephos. The test mortality to DDT ranged between 75% and 90%. The mortality of Cx. tritaeniorhynchus, Cx vishnui and An. subpictus ranged from 85% to 95%. Cx. tritaeniorhynchus is susceptible to Deltamethrin and Cyfluthrin. Cx. vishnui showed 80 % and 85% mortality to DDT and Malathion respectively.

Animals↗

[Physiological and biochemical bases of xero-tolerance of fungal biocontrol agents and their application].

Hyphomycetes fungal biocontrol agents usually infect host insects by conidial germination and penetration into insect integument under high humidity condition which is generally the restrictive factor for the application of mycoinsecticides in insect control in the field. It is ideal to produce more xero-tolerant inocula (e.g. conidia) of the agents, although the current formulation technology has helped to some degree to reduce the dependence of fungal formulations on the high humidity. This paper reviewed the advance in basic and applied studies on the physiological and biochemical bases of fungal xero-tolerance and its water activity regulation in the past two decades. Possible approaches to make fungal formulations more xero-tolerant were discussed, with an emphasis on the increased accumulation of biologically compatible solutes such as low-molecular polyols and trehalose to the inocula produced for formulation. More efforts are necessitated to understand the mechanisms involved in molecular biology and in regulating the accumulation of the compatible solutes, and to explore for new technology to enhance the xero-tolerance of the fungal agents through biochemical regulation in mass production.

Fungi↗

Midgut antibodies reduce the reproductive capacity of Anopheles stephensi (Diptera: Culicidae).

Rabbits immunized with polypeptides of midgut of glucose fed A. stephensi resulted in high titer of antibodies (10(4)-10(6)) as detected by ELISA. Effect of antisera on fecundity, hatchability and engorgement was investigated. Fecundity was reduced drastically (62.4%). Eight polypeptides were recognized by the antisera raised against midgut tissues viz., 92, 85, 55, 52, 45, 38, 29 and 13 kDa. Cross reactivity of these antibodies with different tissues of A. stephensi as well as different species of Anopheles was also analyzed. The results indicated that anti-mosquito midgut antibodies had the potential to disrupt the reproductive physiology of mosquitoes in view of the present study, there is a need for further investigation with target antigens.

Animals↗

Tissue damage after acute intoxication by polychlorinated biphenyls in cockroaches.

It is common knowledge that polychlorinated biphenyls (PCBs) represent a serious threat to the health of both vertebrates and invertebrates. As far as the former are concerned, especially as regards human beings, a broad literature describes the direct and indirect effects induced by the PCBs on their systems and organs. Among invertebrates, the information available is mostly related to arthropods and is, however, very scarce. The aim of this work was to evaluate the effects of polychlorinated biphenyls (PCBs) on tissues and organs of individuals belonging to a species of Blattaria (Blattella germanica) treated with various doses of this toxic material. The pathologies found became more serious as the dosage increased and were present throughout the entire digestive system, in the fat body and in the male gonads: in these areas cell and tissue breakdown and severely damaged spermiogenesis were observed. In particular, the testes, Malpighian tubules and fat body accumulated an amorphous basophilic PAS-positive substance. Furthermore, the NOS-dependent NADPH diaphorase activity pattern in the retina and optic lobes was more evident in the treated than in the control insects.

Animals↗

[Fleas and diseases transmissible to man].

Fleas are wingless insects that parasitize mammals and more rarely birds. They are able to jump and may bite people. Adult fleas are hematophagous. The impact of fleas on public health is related to their ability to act as vectors for transmission of infectious agents during blood meals. The purpose of this article is to describe fleas and the diseases that they transmit to humans. Special focus is placed on epidemiological aspects.

Animals↗

[Health effects of pyrethrins and pyrethroids].

Pyrethrins, natural extracts of Chrysanthemum cinerariaefolium and Chrysanthemum cocineum flowers, and pyrethroids, synthetic analogues and derivatives of pyrethrins, are powerful insecticides. They are widely used in households and insect control in pets or livestock, in textiles such as carpets, wallpapers, furniture and clothes, as well as in agriculture, forestry and public health services. This article brings a list of pyrethrin and pyrethroid insecticides registered for use in plant protection in Croatia. Pyrethrins and pyrethroids can enter the organism by ingestion (accidental or suicidal ingestion or in food), by inhalation and/or by skin contact. Although these pesticides pose a relatively low risk to mammals due to rapid metabolism with no significant accumulation, they can induce adverse health effects, more often in acute poisoning, but also due to chronic exposure. The primary target of pyrethrin and pyrethroid toxicity is the nervous system, since they act directly on the sodium channels of nerve cell axons, leading to hyperexcitation. Another important toxicological mechanism is allergenicity, which is more pronounced with pyrethrins than with synthetic pyrethroids. Because there is no antidote for pyrethrin and pyrethroid poisoning, treatment is symptomatic and supportive. The article discusses the measures for poisoning prevention and alleviation of exposure to pyrethrins and pyrethroids in occupational settings and in general population.

Animals↗