[Effect of secondary structure of the substrate on the hydrolysis of deoxynucleotides by exonuclease A 5 ].
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Poly(A) degrading activities are detectable in nuclear, nucleoplasmic, cytoplasmic extracts and chromatin preparations from rat liver. More than 75% of the activity has been found within the nucleus; most of the nuclear activity is chromatin bound. Poly(A) degradation appears to occur largely via exonucleolitic cleavage.
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The comparative study of the frequency of DNAase and RNAase and the activity of their formation in 59 strains of anaerobic opportunistic bacteria isolated from traumatic and orthopedic patients and in 154 strains of bifidobacteria isolated from feces of healthy persons has been made. The study has revealed that cultures with exonuclear activity occur among opportunistic bacteria and among bifidobacteria, the representatives of human obligate anaerobic automicroflora. However, in opportunistic bacteria the frequency of the above-mentioned enzymes and the activity of their formation have proved to be significantly higher than in bifidobacteria.
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When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage lambda. Mutants of lambda that are deleted for ea47, a gene of unknown function which is expressed early in the lytic cycle, are not blocked for growth. The blocked-growth phenotype of lambda ea47+ in the presence of AddAB is expressed only when phage DNA replication is permitted.
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