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Results for “DENTAL PULP”

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At least 883 records · Page 49Linked to original sources
PubMed2007

Elevated expression of Cu, Zn-SOD and Mn-SOD mRNA in inflamed dental pulp tissue.

AIM: To determine the mRNA expression levels of copper-zinc superoxide dismutase (Cu, Zn-SOD) and manganese SOD (Mn-SOD) in healthy and inflamed human dental pulp tissue. METHODOLOGY: Sixteen patients with symptomatic irreversible pulpitis (eight females and eight males) were selected for study. Normal healthy pulps were removed from extracted mandibular third molar teeth from 10 systemically healthy individuals (six females and four males). QRT-PCR analysis of Cu, Zn-SOD and Mn-SOD mRNA expression was carried out in 16 cases of irreversible pulpitis and in 10 cases of systemically healthy donors. The Shapiro-Wilk's test was used to test the normality of data, whereas the Mann-Whitney U-test was used to evaluate the significance of the differences between groups. Differences in the expression levels were considered to be statistically significant for P-values <0.05. RESULTS: A significant increase (P < 0.05) occurred in both Cu, Zn-SOD and Mn-SOD mRNA expression in cases of irreversible pulpitis. The increase in Mn-SOD level was significantly higher (P < 0.05) than the change observed for Cu, Zn-SOD. CONCLUSIONS: The development of pulpitis is associated with elevated transcription of both Cu, Zn-SOD and Mn-SOD; pulp tissue inflammation generated higher Mn-SOD transcription compared with Cu, Zn-SOD.

Adolescent↗
PubMed1994

Vasculature of the dental pulp of atherosclerotic monkeys: light and electron microscopic findings.

Arteriosclerosis is a pathological condition in which there is a severe luminal narrowing of arterioles, resulting in tissue ischemia and necrosis. Arteriosclerosis has been reported in the dental pulp of normotensive persons as young as 40 yr of age. To date, no corroboration of these findings have been published. Our study examined pulp from atherosclerotic monkeys for such changes under controlled conditions. The lingual artery and canines from 11 nonatherosclerotic (control) and 11 atherosclerotic (experimental) cynolmolgus monkeys were histologically evaluated with light and transmission electron microscopy. Lingual arteries from controls did not exhibit atherosclerotic plaques, whereas those of the experimental monkeys did show fibro-foamy plaques. However, neither experimental nor control animals exhibited any arteriosclerotic alterations in the pulp. Our study concluded that, in this animal model, which did demonstrate atherosclerosis in the oral cavity, no similar alterations were found in the pulp.

Animals↗
PubMed1991

Application of the MTT colorimetric assay to measure cytotoxic effects of phenolic compounds on established rat dental pulp cells.

The cytotoxicities of various phenolic compounds were examined using the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) colorimetric assay and established rat dental pulp cells (RPC-C2A). The ID50 (50% inhibition concentration) of the phenolic compounds ranged from 0.1 to 10 mmol/L, and the cytotoxicities of the phenolic compounds were related to their phenolic coefficients. The therapeutic index of each phenolic compound was calculated from the ID50 results and from the 50% inhibition concentration for prostaglandin synthesis that has been reported previously. Eugenol, guaiacol, and creosol had therapeutic indices much higher than those of other phenolic compounds examined. This experimental system could be used as a simple preliminary assay to test for toxicity of dental drugs and materials.

Animals↗
PubMed2004

Blood-flow and neurosensory changes in the maxillary dental pulp after differing Le Fort I osteotomies.

PURPOSE: The objective of this study was to examine changes in blood flow and the recovery of sensibility in the maxillary dental pulp after two different types of maxillary osteotomies. Study design Nine patients (C-group) who underwent a combined of Le Fort I and horseshoe osteotomy and 14 patients (NC-group) who underwent a single-segment Le Fort I osteotomy were compared. Thirty-two (C-group) and 54 (NC-group) maxillary incisors were examined preoperatively and at 1-7 days, 14 days, and 3, 6, and 12 months postoperatively. The pulpal blood flow (PBF) was measured by laser Doppler flowmetry, and the pulpal sensibility (PS) was investigated by electrodiagnostics. RESULTS: In both groups, the PBF dropped to its lowest value at 1 day after surgery and increased thereafter. Though a temporary drop of the PBF was observed in the NC-group on day 4, the PS recovered earlier in the NC-group than in the C-group. CONCLUSION: These results suggest that the method of maxillary osteotomy influences the postoperative change of the PBF and recovery of the PS in the maxillary teeth.

Adult↗
PubMed2006

Hypervariable region structure and polymorphism of mtDNA from dental pulp and a family analysis.

Nucleotide sequences of the hypervariable region in the D-loop of mitochondrial DNA (mtDNA) were analyzed using DNA extracted from 140 old dental pulp samples. These sequences were compared with the sequence reported by Anderson et al. Nucleotide substitution in the HV1 region was identified at 77 positions. A C-to-T transition at position 16223 (C16223T) was most frequently detected (77.9%). Fourteen types of C-stretch sequence patterns were detected and the same sequence as Anderson had the highest frequency (57.9%). In the HV2 region, base transitions were identified at 56 positions. A263G was identified in all samples. Seven types of C-stretch were detected, but none had the same sequence as Anderson. In the HV3 region, base transitions were identified at 21 positions. T489C was most frequently identified (64.3%). Five types of C-stretch were detected, and the same sequence as Anderson accounted for 92.9%. The 140 samples were classified into 128 kinds by the sequence patterns of the HV region. Next, using the blood and oral mucosa epithelium from 23 subjects comprising four generations in a family line, the hereditary relationship of mtDNA was examined. All mtDNA types of the first-generation mother were infallibly inherited by the fourth generation.

Adult↗
PubMed2006

Cluster analysis and gene expression profiles: a cDNA microarray system-based comparison between human dental pulp stem cells (hDPSCs) and human mesenchymal stem cells (hMSCs) for tissue engineering cell therapy.

We investigated gene expression patterns and functional classifications regarding the clusters of human dental pulp stem cells (hDPSCs) and human mesenchymal stem cells (hMSCs)--which possess a multipotent ability--because little is known about the precise moleculobiological clues by which these cells activate their differentiating ability or functionality to eventually form dentin and bone, respectively. We first verified the expressions of the alkaline phosphatase (ALP) gene, dentin matrix protein 1 (DMP-1), and dentinsialophosphoprotein (DSPP) by real-time reverse-transcriptase polymerase chain reaction (RT-PCR) and consequently discovered the high expressions of these genes. Total RNA was also followed by hybridization with a human microarray system consisting of 12,814 genes. Analyses of gene expression patterns indicated several genes which encode extracellular matrix components, cell adhesion molecules, growth factors, and transcription regulators. Functional and clustering analyses of differences in gene expression levels revealed cell signaling, cell communication, or cell metabolism. In the future, information on the gene expression patterns of hDPSCs and hMSCs might be useful in determining the detailed functional roles of the relevant genes and applicable to stem cell therapies, and these cells could also be used as multipotent cell sources for gene technology and tissue engineering technology.

Adult↗
PubMed1977

Abnormal immune response to infection of the dental pulp. Report of a case.

The following case illustrates an unusual response by the dental pulp to caries in an immunodeficient patient. The patient, a 14-year-old boy, suffered from thymic dysplasia and IgA deficiency. Deep dentinal caries produced relatively little destruction of the pulp and only a mild inflammatory response, in spite of the presence of a large number of bacteria in the pulp chamber. The patient's immunologic disorder was diagnosed as hereditary combined immunodeficiency of unknown type. However, it may have been a variant of Nezelof's syndrome.

Adolescent↗
PubMed1998

Thrombin-induced DNA synthesis of cultured human dental pulp cells is dependent on its proteolytic activity and modulated by prostaglandin E2.

To clarify the roles of alpha-thrombin and prostaglandin E2 (PGE2) in the healing and inflammatory processes of dental pulp, their effects on the DNA synthesis of human pulp cells were investigated by measurement of [3H]thymidine incorporation. At a concentration range of 1 to 25 units/ml, alpha-thrombin stimulated DNA synthesis of the pulp cells by 1.5 to 2.6-fold. On the contrary, PGE2 (> 0.05 microgram/ml) suppressed DNA synthesis by 24 to 39%. Using reverse transcriptase-polymerase chain reaction, thrombin receptor mRNA expression was identified in the pulp cells. Furthermore, alpha-thrombin-induced DNA synthesis could be inhibited by antithrombin III (2 units/ml) with heparin (2 units/ml) or D-Phe-Pro-ArgCH2Cl (50 micrograms/ml). PGE2 (0.1 to 0.5 microgram/ml) also inhibited the thrombin-induced DNA synthesis by 39 to 64%. These results imply that pulp cells express the thrombin receptor that is activated by the serine protease activity of thrombin. Interactions of thrombin and PGE2 are important in modulating the inflammatory and healing processes of the pulp.

Cells, Cultured↗