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Hysterectomy: improving the patient's decision-making process.

Increasingly, patients are expecting to be more involved than they traditionally have been in medical and surgical decision making. The unilateral process of informed consent is evolving into one of informed collaborative choice. Hysterectomy is a procedure that is frequently performed when reasonable surgical and nonsurgical alternatives remain. When professional consensus as to the clear recommendation for hysterectomy is not present, patient choice is particularly important. Because more than 80% of health-care decisions, including those in which one of the choices is hysterectomy, are elective, gynecologists and other health care providers increasingly will need to develop more efficient and collaborative methods to integrate patient autonomy and choice into the decision-making process. There is mounting evidence that both clinical and nonclinical outcomes (satisfaction and cost) may be improved when properly informed consumers collaborate in making medical and surgical decisions. Legal liability for adverse outcomes may be decreased by increased patient participation in medical and surgical decision making. The era of managed care has created an agency problem stemming from the fact that consumers (patients) are concerned that necessary procedures and other treatments may be withheld because of cost considerations. Health plans and medical groups likely will be required to provide objective information about the options that consumers (patients) have when faced with choices, including decision making and hysterectomy. By incorporating patient expectations and preferences as part of the process of decision making, an ethically acceptable and effective method of "rationing by patient choice" may be feasible. Figure 3 is a graphic depiction of such a process of informed collaborative choice progressing from effective choices through efficient choices and then to the one providing the best value for an individual patient.

Cysts↗

[An experience with public education in nutritional health in 2 communities in Jalisco, Mexico].

This study describes a popular educational process conducted in two communities in Jalisco, Mexico. The purpose was to add an alfalfa concentrate to the population's diet as an alternative, locally available food source. Previous studies had shown that alfalfa contains high protein, vitamin, and essential amino acid levels and can be useful to supplement and improve child nutrition. This resource had not been used previously due to lack of knowledge concerning its properties and harvesting and processing procedures and because it had traditionally been used as livestock feed. The current study included four steps: 1) community knowledge, 2) a community survey using interviews, home visits, and child nutrition evaluation, 3) formation of work groups in a community meeting, and 4) an educational program, working with a self-diagnostic tool taking child nutritional status into account. Our work focused on two areas simultaneously: family nutrition and the alfalfa concentrate as a way to improve it. Although this process was lengthy, it resulted in the acceptance and inclusion of alfalfa concentrate. In addition, the community groups formed in the process remain as an ongoing organizational resource.

Diet↗

When pol I goes into high gear: processive DNA synthesis by pol I in the cell.

Pol I is the most abundant polymerase in E. coli and plays an important role in short patch repair. In accord with this role in the cell, the purified polymerase exhibits low processivity and high fidelity in vitro. Pol I is also the polymerase responsible for leader strand synthesis during ColE1 plasmid replication. In a previous publication, we described the generation of a highly error-prone DNA polymerase I. Expression of this mutant Pol I results in errors during the replication of a ColE1 plasmid. The distribution and spectrum of mutations in the ColE1 plasmid sequence downstream the ori indicates that Pol I is capable of more processive replication in vivo than previously accepted. Here, we review evidence suggesting that Pol I may be recruited into a replisome-like holoenzyme and speculate that processive DNA replication by Pol I may play a role in recombination-dependent DNA replication in the cell.

Chromosomes↗

[Maternal transition in mothers with high risk newborns].

PURPOSE: The purpose of this study was a comprehensive understanding about maternal transition in mothers with high risk newborns according to the degree of situational meaning. METHOD: A methodological triangulation that combines qualitative and quantitative methods was used. The situational meaning of a high risk newborn mother was identified using a Family Meaning Attribution Scale. According to the degree of situational meaning, in-depth interviews were conducted at 3 time periods postpartum : between 3-10 days after childbirth, around the time of the newborn's discharge, and between 10-12 weeks after childbirth. Quantitative data was analyzed using descriptive statistics and t-test. Qualitative data was analyzed using Tutty, Rothery, & Grinnell's methodology. RESULT: The average score of the situational meaning in high the risk newborn mother was 53.57(possible score is between 0-96) and the average score of each item was 1.67. A Maternal transition process in the mother that has a positive situational meaning was conceptualized in three distinctive phases : confusion, accepting, and shaping phases. The Maternal transition process in the mother that has a negative situational meaning was also conceptualized in three distinctive phases : avoiding, conflicting, and accepting phases. CONCLUSION: It is necessary that the nurses provide high risk newborn mothers with individualized care considering both the situational meaning that is attributed to them and the maternal transition phase that they are faced with.

Adaptation, Psychological↗

Delivery of laboratory data with World Wide Web technology.

We have developed an experimental World Wide Web (WWW) based system to deliver laboratory results to clinicians in our Veterinary Medical Teaching Hospital. Laboratory results are generated by the clinical pathology section of our Veterinary Medical Diagnostic Laboratory and stored in a legacy information system. This system does not interface directly to the hospital information system, and it cannot be accessed directly by clinicians. Our "meta" system first parses routine print reports and then instantiates the data into a modern, open-architecture relational database using a data model constructed with currently accepted international standards for data representation and communication. The system does not affect either of the existing legacy systems. Location-independent delivery of patient data is via a secure WWW based system which maximizes usability and allows "value-added" graphic representations. The data can be viewed with any web browser. Future extensibility and intra- and inter-institutional compatibility served as key design criteria. The system is in the process of being evaluated using accepted methods of assessment of information technologies.

Animals↗

Building peace from scratch: some theoretical and technological aspects.

A peace-building process is based on activity, acceptance, understanding of political reality, communication, and empowerment. Acceptance means accepting everybody as he or she is and let each know it. This is at the heart of peace work, it is the prerequisite for effective communication, and includes accepting other even in cases of severe disagreement. Peace work requires both an understanding of political reality and the expression of one's own political opinion. Acceptance and the expression of political opinion are not at variance but complementary. Combining acceptance and understanding of the political context provides hope for real communication in which messages are both sent and received, with appreciation and interest. Empowerment implies overcoming of the feeling of powerlessness, often present in conflict by all sides and in all social groups. It includes recovery of self-respect and respect for others. Education and economic independence are important facets of the empowerment concept. Essential principles of peace-building process are responsibility, solidarity, cooperation, and nonviolence. Responsibility encompasses caring for human rights, the suffering of others, and for consequences of our own intended and unintended actions. Solidarity allows learning through listening and understanding. Even with the best intentions on both sides, cooperation may be difficult and painful. Nonviolence is a way of life.

Attitude↗

Graft ischemic time and outcome of lung transplantation: a multicenter analysis.

RATIONALE: The effect of graft ischemic time on early graft function and long-term survival of patients who underwent lung transplantation remains controversial. Consequently, graft ischemic time has not been incorporated in the decision-making process at the time of graft acceptance. OBJECTIVES: To investigate the relationship between graft ischemic time and (1) early graft function and (2) long-term survival after lung transplantation. MEASUREMENTS AND MAIN RESULTS: The data from 752 patients who underwent single lung transplantation (n = 258), bilateral lung transplantation (n = 247), and heart-lung transplantation (n = 247) in seven French transplantation centers during a 12-year period were reviewed. Independent data quality control was done to ensure the quality of the collected variables. Mean graft ischemic time was 245.8 +/- 96.4 minutes (range 50-660). After adjustment on 11 potential confounders, graft ischemic time was associated with the recipient Pa(O2)/FI(O2) ratio recorded within the first 6 hours and with long-term survival in patients undergoing single or double lung transplantation but not in patients undergoing heart-lung transplantation. The relationship between graft ischemic time and survival appears to be of cubic form with a cutoff value of 330 minutes. These results were unaffected by the preservation fluid employed. CONCLUSIONS: The results of this large cohort of patients suggest a close relationship between graft ischemic time and both early gas exchange and long-term survival after single and double lung transplantation. Such relationship was not found in patients undergoing heart-lung transplantation. The expected graft ischemic time should be incorporated in the decision-making process at the time of graft acceptance.

Adult↗

Chloride and calcium in Photosystem II: from effects to enigma(*).

This minireview focuses on the early evidence for roles of chloride and calcium ions in reactions of photosynthetic electron transport and on the reluctance with which an essential function of these inorganic ions in the process of water oxidation was accepted. For example, Daniel Arnon's group initially refuted the conclusion of Otto Warburg, the discoverer of a 'chloride effect,' that chloride was a 'coenzyme.' Their reasoning was that chloride had not been shown to be an essential mineral nutrient of plants. In the case of calcium, the problem was that the first 'calcium effects' had been seen with preparations from cyanobacteria rather than from green plants. While today the status of calcium and chloride as essential participants in the process of water oxidation is rarely disputed, the nature of their involvement still eludes all experimental inquiries. Substantial progress in this respect may come from recent refinements of the application of Fourier-transform infrared spectroscopy to the study of photosynthetic water oxidation.

Journal Article↗

Feasibility and patients' acceptance of Home Automated Telemanagement of oral anticoagulation therapy.

We developed the Home Automated Telemanagement (HAT) system for patients on oral anticoagulation therapy. It consists of a home unit, HAT server, and clinician unit. Patients at home use a palmtop or a laptop connected with a prothrombin time (PT) monitor. Each HAT session consists of self-testing, feedback, and educational components. The symptom data and PT/INR from patient homes are automatically sent to the HAT server and analyzed by the system. Patients who were seen in the Anticoagulation Clinic (N=29) were asked to use HAT in a laboratory setting. Semi-structured, in-depth interviews were used for the evaluation of HAT acceptance. 93% claimed that they would use such a tool at home and would advise other patients to use HAT for self-management and disease-specific education. Twelve patients used HAT at home for eight weeks. Compared to baseline, patients completing the home study showed statistically significant improvement in disease-specific quality of life dimensions of general satisfaction, self-efficacy, daily hassles, and distress. The Client Satisfaction Questionnaire demonstrated significant improvement in patient satisfaction with the treatment process. Our results demonstrated high acceptance of the HAT system by patients receiving long term anticoagulation therapy regardless of their previous computer experience or socioeconomic background.

Administration, Oral↗

Dietitians employed by health care facilities preferred a HACCP system over irradiation or chemical rinses for reducing risk of foodborne disease.

OBJECTIVE: To survey dietitians in health care facilities about the acceptability of alternative meat and poultry processing methods designed to reduce the risk of foodborne disease and their willingness to pay for these processes. SUBJECTS: A geographically representative sample of 600 members of The American Dietetic Association who work in health care facilities. The response rate was 250 completed questionnaires from 592 eligible subjects (42%). DESIGN: A mail survey was used to gather information on the acceptability of a Hazard Analysis and Critical Control Point (HACCP) system, chemical rinses, and irradiation for increasing the safety of food. Discrete choice contingent valuation was used to determine the acceptability at current prices and at 5, 10, and 25 cents per pound above current prices. STATISTICAL ANALYSES: Logistic regression was used to estimate mean willingness to pay (the maximum amount respondents are willing to pay) for each process. A simultaneous equations regression model was used to estimate the effects of other variables on acceptability. RESULTS: Respondents expressed a high level of concern for food safety in health care facilities. The estimated mean willingness to pay was highest for a HACCP system and lowest for chemical rinses. CONCLUSIONS/APPLICATIONS: The successful adoption of alternative methods to increase food safety depends on their acceptance by foodservice professionals. The professionals sampled were most accepting of a HACCP system, somewhat less accepting of irradiation, and least accepting of new chemical rinses. Poultry and beef processors and government agencies concerned with food safety may want to take into account the attitudes of foodservice professionals.

Animals↗

Early object relations into new objects.

Two strands of change are suggested by this review, one maturational, the other therapeutic or developmental (Hartmann and Kris, 1945). By "maturational" I mean to suggest energies that infuse the individual from earliest life in a manner that includes object relations, but for the healthy exercise of which object relations per se need not be of central and crucial importance. Within wide limits such energies may be delayed until growth conditions prevail without significant distortion of certain of the organism's ego functions. Therapeutic change is analogous to developmental change in that both involve the crucial presence of another to release energies. In therapeutic change these are energies that have been repressed beyond the reach of developmental dynamics. In everyday development crisis and synthesis alternate in conjunction with new and emerging objects to add to the psychological structures brought to the fore by maturation. In many instances, as we see with John, over time and in a less focussed manner, developmental changes can approximate therapeutic change and visa versa. Freud-Dann in their "experiment" pursued one line, in which the equipmental delay brought on by extremely adverse living circumstances was redressed by providing an interpersonally enriching, loving, developmentally facilitating milieu. The sketches of individual children and John's subsequent story provide a perspective into what becomes the stuff of growth and what remains the stuff of neurosis. The developmental reserves and ego resilience of these children were impressive but probably not extraordinary. Usual growth ensued as soon as they were provided with the rich soil of Bulldogs Bank instead of the desert sand of the Tereszin concentration camp. However, no one can escape such adverse circumstances without having taken in the stuff of neurosis. Affects and percepts that were not assimilatable or even available to consciousness at the time remain buried in the unconscious. Pain deprived of meaning is buried as neurosis. As we see in John's story, experience that cannot be integrated at the time is locked away from whatever developmental progression has occurred. Intolerable affects and ideas require particular circumstances of object relation and verbalization such as are found in the context of psychoanalysis and arrived at through psychoanalytic interpretation. Or, as in John's case, they may give way only slowly and irregularly over long stretches of time, when subjected to life experiences in the company of new object relations. Broadly stated, the Freud-Dann paper helps us to appreciate that there are several pathways of protection and growth in the ego that involve the discovery or construction of new objects. Family-romance fantasies are a common manifestation of new-object phenomena. Transitional object phenomena are also related. For some individuals at a particular time or over a span of time, providing the right circumstances for the resumption of maturational and developmental growth is all it takes to make them whole. Changes in the adaptive ego are sufficient to alleviate the conflicts stemming from the neurotic ego. For others, depending upon the degree of their neurotic impairment, or for the same individual under other circumstances, therapeutic change in the deepest sense demands the relatively unconditional presence of the interactive and interpreting other. Children of the storm who come in for shelter and warmth may thrive, but they also require a means of getting at the storm in their core that has been internalized as part of the ego's survival mechanism. What can be extracted from the poignant story of the Bulldogs Bank children about current child-analytic technique? The psychoanalytic piano now may be more formally conceptualized as having white as well as black keys. Most analyses, adult and child, have been conducted as though the "black keys"--pressure to mastery through repetition and its subsequent interpretation in relation to the transference--were the sole agents of therapeutic change. Reviewing maturation and development in relation to the resumption of psychological growth suggests that the provision of a beneficient environment, the "white keys," may lead to the resumption of maturational growth and change. The difference between the two modalities would be in the relative need for a significant other to bring about such change. Expanding on Hartmann and Kris, we can say that maturation requires a certain level of human stimulation and a supportive environment to unfold. At times in our work we encounter a psychoanalysis of and about maturation rather than primarily transference and interpretation. By and large the structure and functions of the ego that have been impeded in their exercise by traumatic circumstances in the environment are reactivated by a generalized holding environment rather than a relationship. In the practice of psychoanalysis this means that the child analyst may be more relaxed about the nonverbal play and relational aspects of the work; he need not fear that dynamics not captured in secondary process are lost to change. To the extent that the analysis provides an opportunity for maturational expression, growth will occur. When growth has been impeded by direct and significant interpersonal factors, the standard interpretative clarifications of defense, drive, and object relations in the context of removing the transference distortions regarding the analyst (and the world) are essential for recovery. Where the sequence of repetition through practice to mastery has become frozen by thwarting and stunting relationships, these potentially dead-end examples of neurotic object constancy must be played out on the "black keys." The amalgamation of the black of transference developments and the white of maturational emergence is paradigmatic for the discovery of new objects and new senses of self. In everyday life and analysis, maturation may lead to dramatic change that has hitherto been poorly identified and conceptualized. In child analysis, play is the medium for picking the lock of both arrested maturation and stultified development. Realizing that should permit the child analyst to engage more freely with the child in their own style of play without being overly concerned about the presence or absence of relational dynamic material. Non-dynamic play may not usually be defensive play, though it has often been misinterpreted as such. It may represent activity supporting renewed maturation--practice play in the service of memory, motility, or small or large motor function rather than in the service of the playful, repetitive externalization of threatening introjects. Dynamic play highlights the stagnant or emerging functions of the ego with regard to defense and affect management, which may then be interpreted using the play as key or using words as key to unlock the troubled relationship that is being dramatized. The analyst remains in a non-defensive stance, assigned by the analysand as audience or benign participant. Similarly, in adult analyses some individuals come pre-programmed not so much for interpretation as to discover the analyst as new object. They are in search of near psychobiological maturational closure on an object, already constituted in fantasy, that is respectful, attentive, objective, interested, and sometimes enlightening in their attempts at analytic understanding and developmental homeostasis. This phenomenon is akin to love at first sight, though without the flagrant libidinal romantic element. It occurs as the analytic narrative unfolds and the patient comes to a new sense of self, often around some developmental role or mix of roles, such as spouse, lover, mother, student, sibling, or worker. The point is that playing on the black and white keys of development and maturation leads to the appreciation of a psychoanalytic instrument that is at once more complex and yet easier to get music out of. And development continues from early objects to new objects. New and renewed understandings of analytic events necessarily guide the analyst in the timing of his traditional activities of attending, listening, talking, and relating. A contemporary surge of clinical understanding has led to a more active and informed relatedness on the part of the analyst that allows for a more compassionate approach to verbalization, whether with adults or children. We now know that not every word and every dynamic needs to be funneled through interpretation. The spontaneous powers for recovery that are stimulated by the analytic ground and the analytic process may come to be more accepted as a component of therapeutic gain. Appreciation of the balance of power between the verbal and nonverbal aspects of the analytic process in bringing about therapeutic change has increased. This has led to a greater parity of power and responsibility in the therapeutic alliance. The idea of a "tilted partnership" in which both members work for or against the powerful forces of the analytic process, or of a reciprocal relationship between analyst and analysand has become available to replace the former emphasis on the "tilted relationship." The analyst need no longer be so much in charge of the proceedings whether through deep interpretations of the unconscious or by obsessive attention to associational detail. The ongoing process of developing a body of theoretical and technical understanding that is both reliable and plastic demands an openness that at times flies in the face of the imperative needs of our patients and our profession for clinical confidence and certainty. The analytic clinician, part artist and part scientist, is forever struggling to balance the interminable task of culling new understanding from experience while imposing previously derived understandings that while sure are yet subject to changes stimulated by analytic experience. (ABSTRACT TRUNCATED)

Child↗

The time relationship of performance and audit feedback on conformance with emergency department process criteria.

Adherence to physician-developed process criteria is critical to the medical and legal acceptance of algorithm-directed nonphysician care of acute non-life-threatening illnesses seen in the emergency department. It is generally assumed that adherence to prescribed medical process criteria results in acceptable patient outcomes. We evaluated changes in compliance with varying time delays in audit feedback and varying degrees of supervision. Our evaluation indicated that, under ideal circumstances of daily audit and supervisory feedback, a conformance rate of 80% was achieved. This is a 100% improvement over a group in which neither element was operative.

Acute Disease↗

Improving the process quality using statistical design of experiments: a case study.

A technique known as Statistical design of experiments is a powerful technique for process characterization, optimization, and modeling. It has been widely accepted in manufacturing industry for improving product performance and reliability, process capability, and yield. This article illustrates the application of statistical design of experiments based on the Taguchi approach in a certain company that manufactures electromagnetic clutch coils. The objective of the study was to improve the quality of the existing process and thereby achieve heightened customer satisfaction for the product. An eight-trial experiment was conducted with the aim of reducing the number of rejects from the process. The expected savings per month was estimated to be over $11,500. The results of the study have provided a greater stimulus for the wider application of statistical design of experiments in other core processes within the company.

Analysis of Variance↗

An evaluation of educational outreach to general practitioners as part of a statewide cervical screening program.

OBJECTIVES: The purpose of this study was to determine the acceptability, effectiveness, and cost of a face-to-face educational outreach intervention in the context of a program aimed at increasing cervical screening in Victoria, Australia. METHODS: All identified general practitioners in a specified intervention area were offered a visit by a general practitioner educator. Practitioners completed a questionnaire evaluating the acceptability of the visit. Odds ratios for a woman being screened in the 3 months following the visits were determined. RESULTS: Fifty-nine general practitioners (69.4%) accepted the offer of a visit. Most found both the process and the content of the intervention to be acceptable. The intervention and nonintervention regions did not differ either before or after the intervention. In both regions, there was a statistically significant increase in number of Pap tests performed. There was no difference in the change in screening between the two regions. Costs were estimated at Au$34 per general practitioner visited. CONCLUSIONS: This strategy cannot be recommended for widespread use in a cervical screening program.

Adult↗

Natural history of autografts and allografts.

The clinical outcome of bone grafting procedures depends on many factors, including type and fixation of the bone graft as well as the site and status of the host bed. Bone grafts serve one or both of two main functions, as a source of osteogenetic cells and as a mechanical support. Autografts, both cancellous and cortical, are usually implanted fresh and are often osteogenetic, whether by providing a source of osteoprogenitor cells or by being osteoinductive. The latter is a process whereby the transplanted tissue induces mesenchymal cells of the recipient to differentiate into osteoblastic cells. Cortical grafts, whether autogeneic or allogeneic, at least initially act as weight-bearing space fillers or struts. All bone grafts are initially resorbed, but cancellous grafts are completely replaced in time by creeping substitution, while cortical grafts remain an admixture of necrotic and viable bone for a prolonged period of time. The three-dimensional framework, which supports invasion of the bone grafts by capillaries and osteoprogenitor cells, termed "osteoconduction", is another important function of both autografts and allografts. Fresh allografts are more slowly and less completely replaced by host bones because they invoke both local and systemic immune responses that diminish or destroy the osteoinductive and conductive processes. Although freezing or freeze-drying of allografts improves acceptance, their failure rate is still too high. These processes are also influenced by the vascularity and composition of the host bed. Thus, the interaction of the host and the bone graft determines the success of these procedures, which ultimately is to provide a mechanically efficient support structure.

Animals↗

Multidrug resistance proteins in rheumatoid arthritis, role in disease-modifying antirheumatic drug efficacy and inflammatory processes: an overview.

Drug resistance is generally accepted as an important cause of treatment failure for patients with neoplastic or infectious diseases. Molecular mechanisms underlying drug resistance include the action of drug efflux pumps belonging to the super-family of ATP binding cassette (ABC) proteins, which mediate the cellular extrusion of a large variety of therapeutic drugs, a phenotype that is referred to as multidrug resistance (MDR). Unlike neoplastic and infectious diseases, chronic inflammatory diseases have received little attention. The potential role of ABC transporters in determining the efficacy of anti-rheumatic drugs, notably disease modifying anti-rheumatic drugs (DMARDs), in patients with rheumatoid arthritis is unclear. Based on knowledge from the field of oncology and immunology, this review concentrates on the pharmacological role of MDR proteins in the (clinical) efficacy of several DMARDs, as well as the physiological role of MDR proteins in transporting signalling molecules important in inflammatory processes.

ATP Binding Cassette Transporter, Subfamily B↗

Impartial judgment by the "gatekeepers" of science: fallibility and accountability in the peer review process.

High publication demands and the low acceptance rate of peer review journals place the journal editors and their reviewers in a powerful position. Journal reviewers have a vital role not only in influencing the journal editor's publication decisions, but also in the very nature and direction of scientific research. Because of their influence in peer review outcomes, journal reviewers are aptly described as the "gatekeepers of science." In this article we describe several pitfalls that can impede reviewers' impartial judgement. These include such issues as confirmatory bias, the negative results bias (the file drawer problem), the Matthew effect, the Doctor Fox effect, and gender, race, theoretical orientation, and "political correctness." We argue that procedures currently used by many professional journals, such as blind or masked review, may not completely alleviate the effects of these pitfalls. Instead, we suggest that increasing reviewers' awareness of the pitfalls, accountability, and vigilance can improve fairness in the peer review process. The ultimate responsibilities belong to the journal editors who are confronted with the difficult task of satisfying journal readers, contributors, reviewers, and owners. We recommend that the journal editors conduct periodic internal and external evaluations of their journals' peer review process and outcomes, with participation of reviewers, contributors, readers and owners.

Judgment↗

[Critical control points in the pasteurized milk processing fluxogram].

With the aim of evaluating the microbiological quality of raw and pasteurized milk and confirm the critical control points in a dairy processing industry (Recife-PE, Brazil), 84 samples of milk were collected from four points of the flow-chart considered critical (raw milk storage tank, outlet of the pasteurizer unit, pasteurized milk storage tank and packing filling machine). All samples were evaluated for the presence of mesophilic and psychotrophics microorganisms. The most probable number (MPN) of total and faecal coliforms were determined. Samples of raw and pasteurized milk (collected from the packing filling machine) were also assessed for the presence of Staphylococcus aureus. The preservation temperature of raw and pasteurized milk in the storage tanks and the MPN of total and faecal coliforms present in the water used by the industry were simultaneously evaluated. High counts of mesophilic and psychotrophics microorganisms, total and faecal coliforms and S. aureus were detect in raw milk production and processing. The pasteurization process reduced the microorganisms to acceptable numbers in the Brazilian standards, however, the statistics analysis showed that the number of microorganisms increased significantly (p < 0.01) in the pasteurized milk storage tank, representing an important point of contamination. The probable reason for this findings was the presence of coliforms in 60% samples of water which was used to clean the milk equipment and the inadequate temperatures in the storage tank. Therefore, the results of the microbiological analysis confirm that the milk maintenance temperature in the storage tanks, the pasteurization and the packaging are critical control points in the pasteurized milk processing fluxogram.

Animals↗