SACBROOD VIRUS OF THE LARVAL HONEY BEE (APIS MELLIFERA LINNAEUS).
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Drones, attracted to an observation site by a scented lure, left the lure to fly directly towards a small object suspended nearby. An object the size of a queen bee was detected at distances up to 2 m. It is estimated that at this maximum distance the object will have decreased the light incident on a single rhabdom by 6%.
One-hundred and twenty isolates of lactic acid bacteria isolated from ogi and three traditional cereal-based alcoholic beverages from Nigeria, together with 18 reference strains from Swedish sour doughs, and 50 type-and reference strains of mainly Lactobacillus, were phenotypically classified on their fermentation ability of 49 carbohydrates, including soluble starch. Data were examined by Jaccard Coefficient (SJ), Simple Matching Coefficient (SSM), and unweighted pair group algorithm with arithmetic averages (UPGMA). Seven major clusters were defined at the 82% SJ-similarity level (corresponds to the SSM-level of 91%). Three were identified as Lactobacillus plantarum or L. plantarum-like (together 41% of the ogi isolates). The others were obligately heterofermentative; Cluster 7 was identified as L. confusus (11% of the ogi isolates). Three minor clusters were identified as L. murinus, L. agilis or L. gallinarium, and Leuconostoc mesenteroides subsp. mesenteroides, respectively. The phenotype of the L. plantarum isolates varied within wide limits. Seventeen isolates possessed starch fermenting capacity. Nine of these were identified as L. plantarum or L. plantarum-like (isolated from ogi); one was identified as Leuconostoc mesenteroides subsp. mesenteroides, and the rest were unidentified non-clustering strains.
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1. Spontaneous activity was recorded from neurons in the second abdominal ganglion of bees. 2. Thirty per cent intensity modulations of the horizontal component of the background magnetic field provoked changes in the firing pattern of single neurons. 3. Two classes of neurons were distinguished and confirmed by statistical analysis. 4. Electron dense material in hairs and in or near the cutex may be single domain (SD) and superparamagnetic (SPM) magnetite. 5. A hypothesis is proposed for magnetoreception. Magnetite would act as an amplifier of the external magnetic induction changes. 6. The amplified magnetic field would influence neuronal elements only in restricted regions near the magnetite.
Slices of drone retina were superfused with a Ringer solution containing 1 mM tetraethylammonium (TEA), and the concentration of this ion in the extracellular space [( TEA]0) was measured with ion-sensitive microelectrodes. A train of light flashes for 90 s caused [TEA] to increase by 48 +/- 4% (S.E.), n = 12. Since water crosses cell membranes more readily than TEA does this indicates a volume decrease of at least 32%. Measurements of Ca2+ activity under similar conditions showed an increase of 32 +/- 4% (S.E.), n = 14. Since this is less than the increase in [TEA]0 it suggests that the total amount of Ca2+ in the extracellular space actually decreased.
The development of a sensitive viroimmunoassay for honey bee cytochrome c and its usage for early detection of caste differentiation is described. Pure honey bee cytochrome c was isolated from workers and used to produce antibodies in rabbits. Bacteriophage T4 was chemically modified by covalent attachment of honey bee cytochrome c using tolylene-2,4-diisocyanate as a cross-linking agent. The immunospecific inactivation of this bacteriophage-cytochrome c conjugate by anti-cytochrome c antibodies can be inhibited by free cytochrome c. In quantitative determinations, 50% inhibition is reproducibly achieved at a concentration of 6 ng/ml (5 pmol/ml) and as little as 0.3 ng/ml (0.25 pmol/ml) could be detected by this system. Cytochrome c concentrations were measured in individual animals and substantial differences corresponding larval stages of worker and queen bees are reported.
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Foraging behavior was evaluated in honeybees trained to fly to a feeder containing sucrose only, 1% ethanol, 5% ethanol, or 10% ethanol. The results indicated that exposure to ethanol disrupted several types of honeybee social behavior within the hive. Consumption of ethanol at the feeding site reduced waggle dance activity in foraging bees and increased occurrence of tremble dance, food exchange, and self-cleaning behavior. These ethanol-induced changes in behavior may reflect effects on the central nervous system similar to the previously observed effects of food poisoning with sublethal doses of insecticides.
Sucrose-induced antennal and proboscis motor responses of the honey bee were comparatively studied using muscle responses. The activity of the proboscis muscle responses was correlated with sucrose stimulus concentration, but that of antennal muscle responses was not. Both responses habituated following repetitive stimulation with a low sucrose concentration and dishabituated upon stimulation with a high sucrose concentration. Despite the different response properties of the responses, their habituation kinetics were indistinguishable. The absence of sensory adaptation was confirmed by taste hair recordings. The potential for habituation was related to the functions of the responses studied. The exploratory antennal response used for continuous sampling of the environment habituated to a smaller extent than the appetitive proboscis response.