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Results for “ANTISEPSIS AND ASEPSIS”

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PubMed1976

[Behavior of IgG, IgA and IgM in aseptic and septic postoperative processes].

After sterile surgical procedures, serum IgG, IgA and IgM concentrations again reach normal values at the 10th postop. day after initial lowering. During septicaemia this decrease is significantly more pronounced. By i.v. application of a high dose of human gammaglobulin normal values can be reestablished.

Aged↗
PubMed1989

Surgical wound infections.

At the last meeting of the SURGIKOS Operating Room/Infection Control Combined Advisory Panel, guest speakers reviewed current thinking and practices that have an impact on surgical wound infections. Presentation topics included the role of the CDC, nursing and classification systems in controlling wound infections; cost effective epidemiologic methods of reducing infections; and outcomes of surgical wound infection prevention practices deemed critical by the Joint Commission on Accreditation of Healthcare Organizations. The following is a brief overview of the presentations that guided panel discussions on these topics.

Antisepsis↗
PubMed1975

[Wound care].

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Antisepsis↗
PubMed1976

[Sterility].

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Antisepsis↗
PubMed2000

Poor quality of oocytes from Xenopus laevis used in laboratory experiments: prevention by use of antiseptic surgical technique and antibiotic supplementation.

BACKGROUND AND PURPOSE: Episodic phases of continuous poor-quality oocytes obtained from South American Clawed Frogs (Xenopus laevis) often are observed. In publications dealing with the surgical technique of oocyte removal, the frogs' robust constitution and resistance against infections provided by magainins are pointed out. For this reason, clean rather than sterile conditions for the surgical procedure are mostly recommended. However, in most instances, antibiotics are added to the buffer medium when in vitro experiments are performed using oocytes. METHODS: After a long phase of poor oocyte quality at our facility, involving oocytes that had been obtained by use of a "clean" surgical procedure, we subsequently cultured oocytes in a buffer medium containing the three antibiotics: penicillin G, gentamicin, and streptomycin. RESULTS: During DNA injection experiments, the oocytes developed black spots on their surface by postoperative day two. Pure culture of the gram-negative non-fermentative rod Pseudomonas fluorescens was obtained from the impaired oocytes; the isolate was resistant to the three antibiotics. By contrast, after aseptic surgical removal and culture of oocytes in buffer medium containing the antibiotics tetracycline and gentamicin, perfect oocytes without bacterial contamination were obtained. CONCLUSION: Whenever impaired oocyte quality is observed, microbial contamination should be considered as a possible cause.

Animals↗