Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Libraries”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 865 records · Page 48Linked to original sources
PubMed2006

Construction and partial characterization of a Corynebacterium pseudotuberculosis bacterial artificial chromosome library through genomic survey sequencing.

Corynebacterium pseudotuberculosis is a gram-positive bacterium that causes caseous lymphadenitis in sheep and goats. However, despite the economic losses caused by caseous lymphadenitis, there is little information about the molecular mechanisms of pathogenesis of this bacterium. Genomic libraries constructed in bacterial artificial chromosome (BAC) vectors have become the method of choice for clone development in high-throughput genomic-sequencing projects. Large-insert DNA libraries are useful for isolation and characterization of important genomic regions and genes. In order to identify targets that might be useful for genome sequencing, we constructed a C. pseudotuberculosis BAC library in the vector pBeloBAC11. This library contains about 18,000 BAC clones, with inserts ranging in size from 25 to 120 kb, theoretically representing a 390-fold coverage of the C. pseudotuberculosis genome (estimated to be 2.5-3.1 Mb). Many genomic survey sequences (GSSs) with homology to C. diphtheriae, C. glutamicum, C. efficiens, and C. jeikeium proteins were observed within a sample of 215 sequenced clones, confirming their close phylogenetic relationship. Computer analyses of GSSs did not detect chimeric, deleted, or rearranged BAC clones, showing that this library has low redundancy. This GSSs collection is now available for further genetic and physical analysis of the C. pseudotuberculosis genome. The GSS strategy that we used to develop our library proved to be efficient for the identification of genes and will be an important tool for mapping, assembly, comparative, and functional genomic studies in a C. pseudotuberculosis genome sequencing project that will begin this year.

Chromosomes, Artificial, Bacterial↗
PubMed2007

[Construction of phage antibody library with predetermined CDR3 gene and screening of humanized Fab of anti-human integrin alphanubeta3 monoclonal antibody].

AIM: To construct phage antibody library with predetermined CDR3 and to screen humanized Fab of anti-human integrin alphanubeta(3) monoclonal antibody (mAb) by epitope guided selection. METHODS: LCDR3 gene of mAb E10 was inserted into human light chain variable region gene library. Hybrid phage antibody library was constructed by cloning E10 chimeric Fd gene and human light chain variable region gene into pComb3. Humanized light chain gene was obtained by screening against human integrin alphanubeta(3). Likewise, humanized Fab were gained by panning human phage antibody library, which was constructed by cloning humanized light chain gene and human heavy chain Fd gene with E10 HCDR3 into pComb3. RESULTS: Three humanized Fab clones was obtained by screening hybrid phage antibody library and human phage antibody library, which contained 2.1x10(6), 2x10(7) colony forming units, respectively. Indirect ELISA and competitive inhibition ELISA analysis demonstrated that three humanized Fab antibody had specific binding activity with human integrin alphanubeta(3). The strongest anti-human integrin alphanubeta(3) reactive D5 clone was sequenced and sequencing analysis showed that the V(kappa) and V(H) were derived from VKIII and VHI, respectively. CONCLUSION: Humanized Fab of anti-human integrin alphanubeta(3) mAb has been successfully obtained by phage display technology which lays the foundation for further clinical research.

Antibodies, Monoclonal↗
PubMed1991

[Construction of Echinococcus granulosus genomic library].

Using EMBL3 phage DNA as a vector, a perfect genomic library of Echinococcus granulosus from sheep from Xinjiang has been constructed, which contains 1.2 x 10(6) independent recombinants. The main procedure of construction comprised: 1. extraction of Echinococcus granulosus genomic DNA, 2. partial digestion of the extracted E. granulosus genomic DNA with restriction enzyme Sau3Al, 3. harvest of 15-23 kb DNA fragments by electric elution equipment, 4. preparation of EMBL3 phage vector DNA, 5. cleavage of EMBL3 vector DNA with two restriction enzymes Bam HI and Eco RI to remove the central fragment, 6. ligation of the harvested 15-23 kb E. granulosus genomic DNA and the cleavaged vector DNA with T4 DNA ligase, 7. the package reaction of ligated recombinant DNA in vitro with the package protein and with Q359 strain (Spi-) to screen recombinants, 8. identification of the genomic library, 9. hybridization in situ by pSM889 probe, obtaining 6 positive recombinant clones. It was estimated that the E. granulosus genome was about 1.5 x 10(8) bp. A perfect E.g. genomic library is expected to contain 4.6 x 10(4) independent recombinants at minimum. The constructed genomic library has enough independent recombinants (1.2 x 10(6)). This is the first time to establish an E. granulosus genomic library in China. Previous work showed that there existed differences in many aspects, including pathogenicity, among various isolates of E. granulosus from different hosts and areas. We plan to employ this library to screen the E. granulosus intraspecies DNA probe by hybridization in situ. This kind of probe is envisaged to be of advantage for epidemiological investigation of the hydatid disease in China. It also provides a base for researching E. granulosus at the molecular level.

Animals↗
PubMed1991

The impact of economic issues on Nigerian health sciences libraries.

Economic issues are among the most important factors affecting health sciences libraries in Nigeria. These issues are influenced by the political, cultural, geographic, and demographic characteristics of the country. Significant economic issues are the dependence of the national economy on a single commodity, large foreign debt and spiraling inflation, stringent foreign exchange control measures, and inadequate realization by authorities of the role and importance of health sciences libraries. With shrinking budgets, resources, and staff, health sciences libraries can neither grow nor afford library automation. Health sciences librarians must take initiatives for cooperative activities to increase and make the most of resources, pursue nontraditional methods of fund-raising, educate authorities about the role and importance of libraries, and develop and implement a plan for the development and growth of health sciences libraries in the country.

Financing, Government↗
PubMed1991

Hospital libraries in perspective.

The proliferation of hospital libraries since World War II has created a generation of librarians who take for granted the existence of libraries in hospitals. A literature review for the first half of the twentieth century presents a picture of uncertainty and struggle for identity for the hospital library. Then as now, hospital libraries reflect the institutions within which they operate. A brief history of the development of the American hospital provides a context for describing the various roles that the hospital library has played within its parent institution during the twentieth century. Some personal reflections on working in a hospital library are also presented.

Education, Medical↗
PubMed1990

Restructuring support staff classification levels for academic health sciences library positions.

Nonprofessional library support staff traditionally hold what are considered to be low-paying, nonchallenging positions. These negative factors make retaining creative and productive employees difficult. This article outlines the approach taken at the Medical College of Georgia's Robert B. Greenblatt, M.D. Library to devise a structure of library staff positions that becomes progressively more demanding. A new nine-level Library Staff Classification Plan resulted. This plan also enables and encourages employees to acquire more skills and to accept more responsibility in order to qualify for higher-level library positions or to advance their present position to receive comparable rewards. The plan expresses the level of responsibilities expected, the employee qualifications desired, and lists representative duties across the spectrum of typical library tasks.

Georgia↗
PubMed1990

Improving access to computer-based library and drug information services in patient-care areas.

A project to increase access to drug and biomedical information through electronic linkage of drug information and library services to three patient-care areas is described. In February 1987, microcomputer work stations were installed in the Bexar County Hospital District's hospital emergency department, medical residents' office, and ambulatory-care clinic, as well as in The University of Texas Health Science Center's library reference area and drug information service office. Drug information was available on compact disk through the Micromedex Computerized Clinical Information System (CCIS) database, which includes DRUGDEX, POISINDEX, EMERGINDEX, and IDENTIDEX. Each work station was also connected to the library's computer via modem, allowing access to the Library Information System, books, journals, audiovisual materials, miniMEDLINE, and an electronic mail system. During the six-month project, the system was used 5487 times by 702 people. The system was successful in providing drug and other information in clinical settings and in introducing clinical staff members to new information technology. To increase access to the system after the project ended, the CD-ROM version was discontinued, and the distributed tape version of CCIS for VAX computers was added to the library's online information system, making drug information more available throughout the campus and teaching hospitals. In 1988-89 an average of 200 people accessed the tape version of CCIS each month. Although it is difficult to replace the convenience of an onsite library, at least some drug and biomedical information needs in the clinical setting can be met through computer networking.

Computer Communication Networks↗
PubMed1989

Career progression of academic medical library directors.

While females are still underrepresented as directors overall, the results of our survey indicate that in the past ten years female library directors have been hired in numbers nearly matching their overall percentage of the medical library profession. When the personal characteristics of medical library directors are compared by gender, male directors are more likely to be married, have children, and be somewhat younger upon attaining their first directorship. When the professional characteristics are compared, the only notable difference is that a greater portion of males hold a second master's degree. Despite the changing numbers of female library directors in the past ten years, these differences all held constant before and after 1977. Only the number of internal successions to directorship changed over time. The succession patterns of medical library directors now match more closely those of other academic library directors.

Adult↗
PubMed1989

Adapting IAIMS to a hospital library level.

The Children's Hospital of Michigan Medical Library has adapted several of the Integrated Academic Information Management Systems (IAIMS) concepts and implemented them at a hospital library level. These have included features of network development, electronic interfacing and interlinking, and implementing an integrated information system in the library. The library has incorporated several information systems into library operations, including a variety of in-house, local, and national automated systems and telecommunication networks. Hospital libraries can incorporate IAIMS features and promote an institutional framework of interconnecting communication systems and electronic linkages.

Computer Communication Networks↗
PubMed1988

The new Health Sciences Library at the State University of New York at Buffalo.

The new Health Sciences Library at the State University of New York at Buffalo is a harmonious and functional blend of the old and the new. The old is a renovated Georgian style building with formal rooms containing fireplaces, carved woodwork and English oak paneling. The new is a contemporary four-story addition. Through the arrangement of space and the interior design, the new library offers users easy access to services and resources; accommodates the heavy daily flow of users and library materials; provides an environment of comfort, quiet, and safety; and promotes efficient communication among all segments of the library staff. This was accomplished through sound architectural design which included close consultation with the library director and staff during the planning process. The new library is equipped to face the challenge of meeting the needs of biomedical education, research, and clinical programs of the institution and its constituents in the years to come.

Facility Design and Construction↗
PubMed1987

The contribution of hospital library information services to clinical care: a study in eight hospitals.

Hospital health sciences libraries represent, for the vast majority of health professionals, the most accessible source for library information and services. Most health professionals do not have available the specialized services of a clinical medical librarian, and rely instead upon general information services for their case-related information needs. The ability of the hospital library to meet these needs and the impact of the information on quality patient care have not been previously examined. A study was conducted in eight hospitals in the Chicago area as a quality assurance project. A total of 176 physicians, nurses, and other health professionals requested information from their hospital libraries related to a current case or clinical situation. They then assessed the quality of information received, its cognitive value, its contribution to patient care, and its impact on case management. Nearly two-thirds of the respondents asserted that they would definitely or probably handle their cases differently as a result of the information provided by the library. Almost all rated the libraries' performance and response highly. An overview of the context and purpose of the study, its methods, selected results, limitations, and conclusions are presented here, as is a review of selected earlier research.

Allied Health Personnel↗
PubMed1974

The impact of the Northlands Regional Medical Program Library Services.

The most essential components of the former Northlands Regional Medical Program Library Services are reviewed. Their impact is assessed through interpretation of responses to a questionnaire sent to rural institutions that had borrowed one of the NRMP demonstration core libraries. Although the NRMP had thoroughly promoted the need for these institutions to provide information services beyond their own collection, many of them were not ready to support an alternative means of educating library personnel, and had not made use of the extension services provided through either the University of Minnesota Biomedical Library or the Mayo Medical Library. A gradual change was anticipated, however, and some general recommendations are made as an outcome of this study regarding the direction of further efforts towards medical library development in the non-metropolitan areas of Minnesota.

Education, Medical, Continuing↗
PubMed1970

Integrating information services for dental schools into a health sciences library.

Standards for dental school libraries are first discussed, and the actual state of such libraries described. Advantages and disadvantages of combining the dental library with the health sciences library are weighed. A description is given of the experience at UCLA of integrating dentistry into the total Biomedical Library, with details on serial and monograph selection, together with figures on the size of the collection. Services required of a dental library are detailed, and particular services offered by UCLA described.

California↗
PubMed1967

The National Library of Medicine and interlibrary loan.

The National Library of Medicine was a pioneer in the development of the interlibrary loan system. In 1957, the Library instituted the policy of combining loan and photocopy which it follows to this day. There has been a steady rise in the demand for photocopies from the NLM; hence, the advantage of making fuller use of local resources before approaching NLM is stressed. The criteria the Library observes to strike a balance between the national public interest expressed in the Library's statutory mission, and private interests protected by the Copyright Act, are described; but NLM will always be obliged to provide materials difficult to obtain elsewhere. Developing programs under the Medical Library Assistance Act should have considerable impact on the local availability of medical library resources.

Information Services↗
PubMed1983

OCLC book interlibrary loan in a basic-unit hospital library: one year's experience.

Methodist Medical Center of Illinois Medical Library, a "basic-unit" medical library (i.e., not a resource library) in the Regional Medical Library Program recently completed one year of borrowing and lending books using OCLC. Of the books successfully borrowed through OCLC, 79% were obtained from nonmedical libraries. Forming cost-sharing OCLC clusters among basic units makes OCLC an affordable alternative to borrowing books from overburdened medical resource libraries.

Costs and Cost Analysis↗
PubMed1981

An examination of characteristics related to success of friends groups in medical school rare book libraries.

Friends of the library groups traditionally have been considered effective sources of funding for libraries. Empirical evidence was sought to determine the accuracy of this belief for medical rare book libraries. Characteristics of the medical rare book library and of the friends group were examined to identify those which are correlated with successful friends groups. Correlations were found between the amount of money contributed by the friends group and the age of the friends group, the librarian's participation in forming the group, and the amount of money spent by the library on the friends group. Recommendations are made toward a goal of larger donations from friends groups of medical rare book libraries and toward more effective management of those funds.

Financial Management↗
PubMed1995

A new hospital library: a marketing opportunity.

A new or remodeled library presents a unique marketing opportunity for the hospital librarian. Furthermore, a well-designed library markets itself through its convenience, attractiveness, and ease of use. A marketing approach to library planning takes into account needs of users and of library staff and considers the librarian's relations with the architect as well as with hospital employees. This paper describes ways to combine library planning with marketing techniques and specifies aspects of the library that contribute to its good image.

Facility Design and Construction↗
PubMed1993

Human cDNA libraries and brain banks.

Human brain banks are useful sources of RNA for constructing specific cDNA libraries. These cDNA libraries are a powerful tool for the study of genetic diseases and for genome mapping. cDNA libraries can be constructed which contain all the mRNAs expressed in a normal or abnormal brain, rarely expressed mRNAs or brain-specific mRNAS. Several strategies for using human brain cDNA libraries are considered, and different protocols are described for the conservation of human brain tissue samples, isolation of mRNA from these samples, construction of human cDNA brain libraries, and subtraction of cDNA libraries for enrichment in specific cDNA populations.

Brain↗