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At least 865 records · Page 48Linked to original sources
PubMed1988

Antimicrobial drug susceptibility of Leptospira interrogans serovar hardjo isolated from cattle.

The susceptibility to commonly used drugs of 18 isolates of Leptospira hardjo from the kidneys of feedlot cattle from different sources was determined quantitatively. All isolates were susceptible to penicillin G, ampicillin, tetracycline, erythromycin and streptomycin. Susceptibility to sulphamethazine was ambiguous. No drug resistance was detected and the results were similar to those described for other serovars.

Animals↗
PubMed1987

Serologic correlation of suspected Leptospira interrogans serovar pomona-induced uveitis in a group of horses.

After the observation of 2 horses with uveitis on a horse farm in the Minnesota River valley, 100 horses from this geographic area were given ophthalmologic examinations and were evaluated serologically for leptospirosis. A statistically significant (P less than 0.001) association was observed between the finding of antibodies against Leptospira interrogans serovar pomona and uveitis.

Animals↗
PubMed1986

Isolation of Leptospira interrogans serovar bratislava from sows in Iowa.

Leptospira interrogans serovar bratislava was recovered from 2 of 10 sows examined from an Iowa slaughterhouse. Isolations were made from the kidney and genital tract of each sow. Serovar bratislava is not included in vaccines because it has not been previously isolated in the United States.

Animals↗
PubMed1985

Monoclonal antibodies to Leptospira interrogans serovar pomona.

Three monoclonal antibodies produced against Leptospira interrogans serovar pomona have been studied for their diagnostic usefulness. All three monoclonals reacted strongly in the enzyme-linked immunosorbent assay and indirect fluorescent antibody test with serovar pomona and did not react with serovars grippotyphosa, canicola, icterohaemorrhagiae and hardjo.

Animals↗
PubMed1985

[Cloning and the expression of the hemolysin gene of Leptospira pomona pomona in Escherichia coli].

The library of Leptospira pomona genes was obtained on phage vector AL 47.1. From this library a recombinant phage carrying the hemolysin gene was selected. The DNA fragment (7.7 kb) of this phage containing the hemolysin gene was subcloned on plasmid pUC19. E. coli clones with hybrid plasmid pDR7 were shown to be hemolytic, but the secretion of hemolysin by E. coli into the culture medium was not observed.

Cloning, Molecular↗
PubMed1985

Experimental infection of calves with Leptospira interrogans serovar hardjo: conjunctival versus intravenous route of exposure.

Eight-month-old calves, housed under maximum isolation, were exposed to pathogenic Leptospira interrogans serovar hardjo by the conjunctival route or IV. One calf served as an unexposed control. Infection was monitored serologically (microscopic agglutination test and enzyme-linked immunosorbent assay; ELISA) and by leptospiral culture isolation from periodic urine samples and from the kidneys, epididymides, and aqueous humor collected at slaughter. Microscopic agglutination test titers of greater than or equal to 1:40 were detected among all IV exposed calves at postinoculation day (PID) 7 and among conjunctival exposed calves at PID 14. By ELISA, all IV exposed calves were positive by PID 3, whereas conjunctival exposed calves were positive at PID 14. The ELISA was more sensitive for the detection of antibodies against leptospires in cattle. Leptospires were isolated from the urine of 4 calves and from the kidney of 3 calves exposed by the conjunctival route, but not from IV exposed calves. The results indicated that the conjunctival route of exposure was a more natural and successful route for experimental infection of cattle with serovar hardjo.

Agglutination Tests↗
PubMed1985

Excretion of Leptospira interrogans serovar hardjo following calving or abortion.

Leptospira interrogans serovar hardjo was demonstrated in the vaginal discharges of 12 experimentally infected heifers for up to eight days after abortion or calving. Organisms were recovered from the oviducts of heifers examined at slaughter eight to 22 days after calving or 32 to 91 days after infection. They were also recovered from the uteri of four heifers eight to 22 days after calving.

Abortion, Veterinary↗
PubMed1985

Experimental infection of lactating goats with Leptospira interrogans serovars pomona and hardjo.

Pathogenesis of Leptospira interrogans serovars pomona and hardjo was evaluated in 14 lactating goats. Although mild clinical signs of leptospiral infection characterized by pyrexia and reduction in milk yield appeared in some animals, a consistent clinical pattern was not observed in the inoculated animals. The pomona serovar was isolated from the kidney of 1 of the 4 goats inoculated with serovar pomona. The hardjo serovar (strain UI 750) was isolated in the rabbit serum-supplemented bovine albumin polysorbate-80 liquid medium only from the mammary gland of 1 of 4 goats at 13 days after inoculation with serovar hardjo. The positive culture was detected after an 8-month incubation period.

Animals↗
PubMed1985

Experimental infection of pregnant and lactating goats with Leptospira interrogans serovars hardjo and szwajizak.

Pathogenesis of 2 Leptospira serovars, hardjo and szwajizak, was studied in pregnant and lactating goats. Although clinical signs of leptospiral infection were minimal, cultural isolations were made from the mammary gland of 2 goats and the kidney of 1 goat inoculated with serovar hardjo (C846). The isolations were made only on solid bovine albumin polysorbate-80 medium supplemented either with rabbit serum or sodium pyruvate. Cultural isolations of serovar szwajizak were made from kidney, liver, brain, urine, and mammary gland samples of 1 goat and the liver and kidney samples of its kids. These isolations were made in only the solid bovine albumin polysorbate-80 medium which had been supplemented with normal goat serum.

Animals↗
PubMed1985

Presence of zoonotic pathogens (Yersinia spp., Campylobacter jejuni, Salmonella spp., and Leptospira spp.) simultaneously in dogs and cats.

The purpose of this study was to determine the presence of zoonotic pathogens simultaneously in animals. The isolation of human pathogenic Yersinia enterocolitica (Ye), Yersinia pseudotuberculosis (Yp), Campylobacter jejuni (Cj), Salmonella spp. (Sal) and Leptospira spp. (Lep) in 318 cats and 252 dogs were performed in Shimane Prefecture, Japan. A total of 13 isolates of Yp (4 strains) and Sal (9 strains) were recovered from intestine and/or mesenteric lymph nodes (MLN) of 13 cats (4.1%) but not Ye, and Cj was not examined. A total of 88 isolates of Ye (15 dogs, 15 strains), Yp (16 dogs, 16 strains), Cj (10 dogs, 13 strains) and Sal (39 dogs, 44 strains) were recovered from intestine and/or MLN of 76 dogs (30.2%). Two species of Ye O3, Cj and Sal were recovered from either intestine or MLN of 4 dogs but not from cats. Lep was not detected in dogs and cats kidney. The 101 isolates from dogs and cats belonged to Ye 3B/O3/II (biotype/serotype/phage type) (2 strains), 4/O3/VIII (10 strains) and 2/O5,27 (3 strains), Yp serotypes 1b, 2b (each 3 strains), 2c (2 strains), 4a (2 strains), 4b (4 strains), 5a (5 strains) and 7 (1 strain), Cj serotypes TCK 9, 13, 26 (each 1 strain), 21 (2 strains) and untypable (8 strains) and 24 serotypes of Sal. Ye O3 and Yp were detected frequently in cold months. There was no definite seasonal variation of Ye O5,27 Cj and Sal in internal origin of dogs and cats. Ye O3, Yp and Cj were counted at 10(2) to 10(7) cells per g of the jejunal-to-rectal contents, but Sal at less than 10(2) cells per g of the intestinal contents. Ye O3, Yp and Sal were recovered from mesenteric lymph nodes, but not Ye O5,27 and Cj.

Animals↗
PubMed1978

Viability of Leptospira interrogans serotype grippotyphosa in swine urine and blood.

Normal swine urine, devoid of its microbial flora, diminished the viability and virulence of Leptospira interrogans serotype grippotyphosa. Bovine serum albumin diluent effectively offset the urine's deleterious effects. Membrane filtration (0.45 micrometer) rendered urine free of bacteria, but permitted passage of limited numbers of leptospires. Most of the commonly used anticoagulants did not alter viability of grippotyphosa in whole blood. However, EDTA significantly reduced the number of viable cells.

Animals↗
PubMed1974

Electron microscope identification of mesosomes in some water strains of Leptospira by reduction of tetrazolium chloride to formazans.

Rounded granules protruding from the external surface of some water Leptospira strains and considered as mesosomes were studied by electron microscope and by means of the intravital reduction of tetrazolium chloride. Satisfactory results were obtained with two of the strains studied. It appears that neither the culture medium nor the age of the culture have any influence on the unusual position of the mesosomes.

Formazans↗
PubMed1979

A serological survey of Leptospira interrogans serotype pomona in Saskatchewan horses.

Leptospira interrogans serotype pomona antibody titres of 1:100 or greater were detected in 12.8% of 408 adult horses from seven of eight sampled herds in Saskatchewan. The geographical distribution of the seropositive horses was widespread throughout the agricultural area of the province. The geographical distribution and the cumulative increase in prevalence with age suggested that serotype pomona is enzootic in the equine population of Saskatchewan.

Animals↗
PubMed1979

Experimentally induced Leptospira interrogans serovar autumnalis infections in young swine.

Leptospira interrogans serovar autumnalis strain 621 was proven to be infective for swine by intranasal and ocular inoculation and by contact exposure. The course of the disease was that of a chronic asymptomatic infection. Throughout the experiment, leptospires were observed in all tissues examined by darkfield microscopy of 10% tissue suspensions and examination by indirect fluorescent antibody technique of frozen tissue sections. Infected pigs had increasing antibody titers, as determined by the microscopic agglutination test. The organism spread from intranasally inoculated pigs to contact controls.

Administration, Intranasal↗
PubMed1971

Proposed standardization of the agglutination-adsorption test for Leptospira.

The systematics of the Leptospira is based on the agglutination test and on the cross-agglutination-adsorption test. To obtain reproducible and comparable results these tests must be carried out by standard techniques, but the accuracy of the tests is in any case limited since immune sera prepared from animals do not always react uniformly. Moreover, the micro-organisms used in the tests have specific requirements and do not always develop properly in synthetic media. The various factors that influence the results of agglutination-adsorption tests were investigated and evaluated. Taking the results of these studies into account, a standardized method for agglutination-adsorption tests is proposed and described.

Adsorption↗