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Milk production of dairy cows fed wet corn gluten feed during the dry period and lactation.

An experiment was conducted with 36 primiparous and 40 multiparous Holstein cows to examine the effects of feeding wet corn gluten feed (WCGF) on 305-d milk production, dry matter (DM) intake, body condition score (BCS), and health. The experimental treatments included: 1) control--WCGF not fed (n = 27); 2) WCGF-L-cows received diets containing WCGF (38% DM basis) during lactation (n = 23); and 3) WCGF-DL--cows received diets containing WCGF (38% DM basis) during the dry period and lactation (n = 26). During the dry period, cows consuming WCGF were observed to have a significant gain in BCS (0.07 +/- 0.06) compared with a loss in BCS in cows fed the control diet (control = -0.11 +/- 0.06 and WCGF-L = -0.04 +/- 0.06). During lactation, there were no differences by treatment on BCS. Cows consuming WCGF during lactation consumed more feed compared with the control: 25.4, 23.8, and 21.2 +/- 0.76 kg/d for WCGF-L, WCGF-DL, and the control, respectively. Milk production was higher for cows consuming WCGF: 35.0, 34.7, and 31.1 +/- 2.1 kg/d for WCGF-L, WCGF-DL, and the control, respectively. No differences were found in either DM intake or actual milk yield between the WCGF-L and WCGF-DL treatments, indicating that prepartum diets did not influence lactational performance. The WCGF diets resulted in significant reductions in the concentration of milk fat (3.94, 3.74, and 4.15 +/- 0.08% for WCGF-L, WCGF-DL, and the control, respectively), but because total milk yield was increased, there were no differences in total milk fat yield. In addition, 3.5% of fat-corrected milk tended to be affected by diet: 38.9, 36.3, and 34.7 +/- 1.93 kg/d for WCGF-L, WCGF-DL, and the control, respectively. The increasing effect of DM intake and milk yield in cows consuming WCGF resulted in a similar efficiency of 3.5% fat-corrected milk production for all treatments, averaging 1.5 +/- 0.09. Total protein yields were significantly higher for cows consuming WCGF diets during lactation: 1.15, 1.10, 1.00 +/- 0.06 kg/d for WCGF-L, WCGF-DL, and the control, respectively. These results indicate that diets may be formulated to contain as much as 37.5% WCGF (DM basis).

Animals↗

Response of Holstein cows to corn gluten meal used to increase undegradable protein in early or later lactation.

Supplemental corn gluten meal was used to raise CP by 1.1 to 1.5 percentage units and undegradable intake protein from 35 to 39% of CP in the corn-based diet of parity 1 or greater Holstein cows to study effects of undegradability, parity, stage of lactation, and interactions on DMI, milk yield and composition, BW, and related traits during complete lactations. Cows were assigned at calving to treatments (n = 30, 8 primiparous): control, supplement wk 1 to 8 postpartum (early), or supplement wk 9 to 44 postpartum (late). Total lactation means were not affected significantly by treatments. Supplementation with undegradable protein enhanced forage and, thus, total DMI in later lactation by pluriparous cows; it apparently spared BW loss wk 1 to 8 postpartum and enhanced BW recovery thereafter in first lactation cows with no effect in older cows. Effects of supplementation on milk yield were small, and they were negative in early lactation and generally positive in late lactation; effects were positive on fat test in early lactation for both parity categories but distinctly negative for parity 1 cows in late lactation. Supplementation of undegradable protein in late lactation also decreased milk protein content in parity 1 cows and raised it in older cows. Data suggest that Lys may have been first-limiting, followed by Ile in early lactation and Met in late lactation, and that AA adequacy may be more important than undegradability in ration protein balancing. For most traits measured, treatment by parity interactions were significant, indicating that parity 1 cows did not respond in the same way as older ones to protein supplementation.

Amino Acids↗

Growth performance and carcass composition of turkey hens fed corn gluten feed.

Female poults were fed diets containing 0, 10, or 20% corn gluten feed (CGF) from 0 to 16 wk of age or no CGF from 0 to 9 wk and 20% CGF from 9 to 16 wk of age. All diets were formulated to be isonitrogenous and isocaloric, within each age interval. Diets containing CGF had no significant effect on body weight gain, feed efficiency, yield of carcass parts, or carcass composition, as compared with effects of feeding a corn-soybean meal control diet. However, age of slaughter, 14 vs. 16 wk, had a significant effect on the proportion of breast meat, breast bone, thigh skin, thigh bone, and drums. Carcasses were significantly higher in protein and lower in fat at 14 wk than at 16 wk of age. Results suggest CGF can successfully constitute up to 10% of the diet of growing turkey hens. The use of CGF in turkey diets up to this level would be governed by the economics of the nutrient supply. At 20% of the diet, the tendency for CGF to induce moist droppings may impose a restriction on its use, and the degree of restriction would depend on the housing-management situation.

Animal Feed↗

Evaluation of corn gluten feed in the diets of laying hens.

The use of corn gluten feed (CGF) in the diets of laying hens and in induced molting programs was investigated through four experiments. The first two experiments evaluated the effects of CGF on egg production from 23 to 56 or from 32 to 65 wk of age. Graded levels of CGF from 0 to 25% were added to a corn-soybean meal, control diet (16.5% CP) in a nonisocaloric and an isocaloric manner. The ratio for dietary MEn:protein was held constant in some diets, but varied in others. The nonisocaloric inclusion of up to 25% CGF did not negatively affect egg production in either experiment. When 25% CGF was fed to the 32 wk old hens, egg weight was decreased. Egg weight was also decreased when 10% CGF or higher levels were fed to the younger hens. The nonisocaloric addition of CGF also increased feed intake and decreased feed efficiency (gram of egg per gram of feed) in most cases. Isocaloric inclusion of 15% CGF did not affect egg-production parameters, but 25% CGF negatively affected most parameters. When the ratio for MEn:protein of the CGF diets was equal to that of the control diet, 25% CGF negatively affected most parameters, 17.5% CGF decreased egg weight and feed efficiency, and 10% CGF increased egg production. Feed intake was increased at all of the CGF levels. Two additional experiments evaluated CGF in programs for induced molting. The molting procedure involved feed withdrawal for 10 days followed by feeding molt diets consisting of 99.75% corn, 99.75% CGF, 49.75% corn:50% CGF, or two grower-type diets for pullets (15% CP) containing 25% CGF or 18.5% wheat bran. The two grower-type diets for pullets yielded similar and significantly greater postmolt gains in body weight and early postmolt egg production (3 wk) than did the other diets. The corn:CGF diet also yielded greater postmolt weight gains and early postmolt egg production than the 100% corn or 100% CGF diets. Long-term postmolt egg production (30 or 37 wk) was not affected by the molt diets.

Animal Feed↗

Recurrent monoarthritis in an 11-year-old boy with occult coeliac disease. Successful and stable remission after gluten-free diet.

A case of occult coeliac disease (CD) presenting with recurrent monoarthritis in a boy aged 11 years is reported. The case is unique due to the association of occult untreated CD and arthritis in childhood. Peripheral or axial arthritis as a first manifestation of occult CD has been described in adult patients, with an interval between the arthritis and CD of up to 15 years. In our case the interval between the appearance of arthritis and the diagnosis of CD was 2 years. The boy was asymptomatic for bowel disease and his nutritional status was normal. The diagnosis of CD was established using anti-gliadin (AGA) and anti-endomysium (EMA) antibody tests and was confirmed by small bowel biopsy. The introduction of a gluten-free diet resulted in the persistent remission of arthritis. As the treatment of CD-associated arthritis is based on dietary therapy, physicians should be alert to the possibility of occult CD in any child with arthritis of unclear origin.

Arthritis, Juvenile↗

[Performance of rice varieties in making bread without gluten].

The objective of this work was the evaluation of the technological behavior of seven rice genotypes, using a baking test for bread without gluten, and taking account the influence of particle size and physicochemical properties of the rice on the technological aptitude to produce the bread. Total and insoluble amylose content and hydration were used to make its relationship with bread quality. The genotypes Rico and H-144-7 have contributed to give the best results at baking test while waxy rices gave the lower quality bread. Also we emphasize that a certain relationships can be assumed between hydration and insoluble amylose content with the organoleptic evaluation of breads.

Bread↗

[Regression of mesenteric lymph node cavitation syndrome complicating celiac disease after a gluten free diet].

We report the case of a 39-year old woman with celiac disease in association with a cavitating mesenteric lymph node, hyposplenism and intra-hepatic haematopoiesis. The serious initial clinical picture evoked a diagnosis of non-Hodgkin lymphoma but was not confirmed on multiple biopsies. Despite the usual poor prognostic clinical outcome in such a setting, treatment with a strict gluten-free diet resulted in a remarkable persistent improvement in clinical status and lead to almost complete regression in radiological signs observed for up to 30 months follow-up.

Adult↗

[Gliadin determination in gluten-free products by the ELISA method].

Immunoenzymatic method with rabbit antibodies against gliadin and antibodies conjugates with peroxidase for gliadin determination in gluten-free food using polypropylene and polystyrene was successfully used. Results of gliadin determination indicate that content of gliadin in investigated samples was low--about 0.1%.

Enzyme-Linked Immunosorbent Assay↗

Evaluation of meat meal, chicken meal, and corn gluten meal as dietary sources of protein in dry cat food.

The nutritional value of meat meal (MM), chicken meal (CM), and corn gluten meal (CGM) as dietary sources of protein in dry food formulated for adult cats was evaluated. Twelve healthy adult cats (11 males and 1 female) were used. Dry diets containing MM, CM, or CGM as the main protein source were given for a 3-week period in a 3 x 3 Latin-square design. Digestion and balance experiments were conducted during the last 7 d of each period. In addition, freshly voided urine was taken to determine urinary pH and number of struvite crystals. As compared with the CM diet, dry-matter digestibility was higher and lower for the MM and CGM groups, respectively. Percentages of nitrogen (N) absorption and N retention to N intake were higher in the MM group, and N utilization was not different between the CM group and the CGM group. All cats excreted alkaline urine (pH > 7). Urinary pH, struvite activity product, and number of struvite crystals in urine were lower for the CGM group. There was no difference in retention of calcium and magnesium among the groups. From the point of view of digestibility and N utilization, MM is superior to CGM, and CM is better than or equivalent to CGM as a protein source of dry foods for adult cats. However, when CM is used as a dietary protein source, some manipulation of dietary base excess may be needed to control urinary acid-base balance, because CM contains higher calcium and phosphorus.

Animal Feed↗

[Evaluation of mental status of children with malabsorption syndrome after long-term treatment with gluten-free diet (preliminary report)].

The author examined 41 children, suffering from celiac disease with psychiatric methods and EEG. The children, aged 7-17 y., were many years on gluten-free diets. Various psychiatric symptoms were found in 48.8%, and EEG abnormalities in 70.7%. Only 9 children (21.9%) were free from any psychiatric disorders and EEG abnormalities.

Adolescent↗

Bone mineral density in adult celiac patients and the effect of gluten-free diet from childhood.

Peripheral single photon absorptiometry was used to measure forearm bone mineral density in 22 celiacs on gluten-free diet from childhood (male 14, female 8, age 13-20) and 29 untreated adult celiacs at diagnosis (male 5, female 24, age 18-56, 14 with subclinical disease), compared with healthy sex- and age-matched controls. Bone mineral density was similar in patients treated from childhood and their controls [(668.4 +/- 65.3 vs. 654.9 +/- 69.6 mg/cm2, (mean +/- SD)], but significantly lower in untreated patients than in their controls (598.3 +/- 83.1 vs 673.2 +/- 42.7 mg/cm2, p less than 0.001). It was also significantly lower in the 12 younger untreated celiacs (18-28 yr) versus controls (619.4 +/- 68.5 vs 669.1 +/- 39.3 mg/cm2, p less than 0.01). In the untreated women, but not their controls, a negative correlation (p less than 0.05) was observed between bone mineral density and age. Bone mineral density did not correlate with severity of clinical or biochemical abnormalities. These results suggest that bone derangements are common in celiacs diagnosed in adulthood, even if they never presented evident malabsorption symptoms, and emphasize the importance of early diagnosis and treatment.

Absorptiometry, Photon↗

Antigliadin antibodies and gluten-free diet in dermatitis herpetiformis.

IgA and IgG class antigliadin antibodies (AGA) were analysed with ELISA technique from serum samples of 30 dermatitis herpetiformis patients. Jejunal biopsies were performed to all patients before any treatment and high levels of IgA class AGA were found to be associated with subtotal villous atrophy. Fourteen patients started gluten-free diet (GFD) which caused a significant decrease in both IgA and IgG class AGA. The decrease of IgA AGA was faster than that of IgG AGA and IgA antibody levels fell to normal range during the GFD treatment in all but one patient. In contrast, 5 out of 8 patients followed on normal died showed increasing IgA AGA levels and all of them had a rise in IgG AGA. IgA and IgG class antibodies to cow's milk were also measured in these patients but in contrast to AGA the diets had no clearcut effect on these antibodies.

Adolescent↗

[Clinical aspects of cow milk protein and gluten intolerance in children].

In two comparative groups of 50 children with cow milk proteins and 45 children with gluten intolerance retrospective analysis of initial symptoms was carried out. The initial symptoms of intolerance included: vomiting, loss of appetite, recurrent diarrhoea, and weight gain disorders. These symptoms closely correlated with the type of nutrition (mixed, artificial) and the duration of exposition to harmful component of the food. The symptoms appeared within first days after birth with peak intensity in 6-8 weeks of life in the group with cow milk proteins intolerance. The symptoms of intolerance were most frequent in children of group II in 7-12 months of life. To prevent food intolerance in Polish children, it is recommended to feed them naturally as long as possible and to introduce flour and 4 basic grains late (after the 6th months of life).

Animals↗

The effect of gluten on HLA-DR in the small intestinal epithelium of patients with coeliac disease.

Frozen sections of control and coeliac jejunal mucosa were examined for HLA-DR staining. In general the staining pattern in the controls and treated coeliac patients was a patchy subapical pattern on villous tips. Untreated coeliac patients gave a different pattern with staining extending to the crypt enterocytes. Gluten challenge in three treated coeliac patients induced an altered staining pattern within 2 h.

Celiac Disease↗

The effect of gluten-free diet supplemented with Humana-MCT on the level of lipid fractions in blood serum of infants with coeliac disease.

The concentrations of free fatty acids (FFA), triglycerides (TG), free cholesterol (FC) and estrified cholesterol (EC) as well as the fatty acid composition of TG and EC fractions have been determined in blood serum of 16 infants with coeliac disease. The determinations were carried out three times: after admission and diagnosis but before the initiation of any treatment, after two and after four weeks of a glutene-free diet supplemented with Humana-MCT formula. Before treatment all the infants had weight deficite between 20% and 40%. After two weeks of diet treatment, the concentrations of blood serum FFA and TG decreased as compared to those before the treatment. The highest values of EC were found during the third determination. The composition of fatty acids in TG and EC fractions did not change appreciably in the consecutive determinations. The absence of medium chain-length fatty acids in the studied lipid fractions despite steady supply in the diet suggests efficient metabolization of these acids in the liver. A considerable deficite of linoleic acid appearing in the infants studied before the treatment was not fully compensated during the four weeks of diet treatment.

Celiac Disease↗

Variation of serum and intestinal gluten antibody specificities in coeliac disease.

Protein blotting techniques were used to investigate the gluten specificity of IgA and IgG antibodies in sera and intestinal aspirates from patients with coeliac disease and normal controls. Initially, discontinuous SDS polyacrylamide gel electrophoresis was used to separate the flour proteins. All normal and coeliac sera contained antibodies which bound to various of the gliadin proteins. In only a few sera was binding found to the high molecular weight glutenin subunits, while none was detected to the salt-soluble wheat proteins. Polyacrylamide gradient gel electrophoresis was then used to further separate the gliadin proteins. Almost all normal sera examined showed similar gliadin specificity, binding uniformly to all gliadin groups. While approximately a quarter of the coeliac sera showed even binding to all of the gliadin proteins, the majority showed antibody binding intensely to discrete groups of gliadin bands. We were unable to identify any gliadin band(s) which only bound antibodies from coeliac patients in comparison with normal subjects. The specificities of IgG and IgA serum antibodies were identical for each patient examined, but some differences between serum and intestinal IgA specificities were found for certain patients.

Adult↗