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The one-cell mouse embryo bioassay was utilized to test the embryotoxicity of three brands of powerless surgical gloves; Pristine, Ansell, and BioGel. The Pristine gloves demonstrated no significant embryotoxicity, while the other two brands demonstrated a consistent inhibition of blastocyst development of one-cell mouse embryos.
Evidence of glove perforation, and therefore blood contact, was evaluated in 100 consecutive pairs of gloves used in aesthetic surgical procedures. The surgeon (38.3%) was more likely than the assistant (22.5%) to have exposure. In only 15% of these cases were they aware of the exposure. The left index finger (44%) was the most common location of perforation and 29 of the 32 holes (90.6%) were in cases that lasted more than 2 hours.
The aim was to assess the permeation of different herbicide emulsion concentrate formulations of 2,4-dichlorophenoxyacetic acid (2,4-D) as 60.8 and 83.5% butoxyethyl ester (BEE) and 46.8% dimethyl amine salt (DMAS) through four types of glove materials (lined unsupported nitrile, unlined unsupported butyl, Silver Shield laminate, and Viton). This entailed the development of new microchemical techniques to allow sensitive capillary gas chromatography/mass spectrometry (GC/MS) of the permeated herbicide. The 2,4-D DMAS was esterified to the methyl ester by boron trifluoride-methanol complex with 99.2 +/- 3.7% efficiency using microwave heating to minimize reaction time and to process microsamples. The GC/MS detection limit was 5 ng/ml (ppb) of 2,4-D DMAS in the collection medium. The permeates from the ester formulations were analyzed directly for the ester above the detection limit of 9 ng/ml (ppb) BEE. The permeation investigations utilized the American Society for Testing Materials (ASTM)-type permeation cells with liquid collection media. The results showed that these gloves could provide at least 6 h protection for these formulations.
In a single-blind, controlled, prospective trial of 242 sutured wounds of the hand in the A&E department there was increased late purulent infection in those treated without gloves. This difference is statistically significant. We recommend wearing of sterile gloves to suture all wounds in A&E departments.
We studied 143 men who underwent transrectal prostatic biopsies using the double-glove technique. No patient received any antimicrobial therapy before the procedure. Clean catch urine cultures were obtained at admission and two, four, and twenty-four hours, and two weeks after biopsy. Aerobic and anaerobic blood cultures were performed at admission, and at thirty minutes and four hours after the procedure. In addition, clinical parameters were monitored closely in the hospital for twenty-four hours after the biopsy. A total of 132 patients were considered evaluable. Temperatures of 37.6 degrees C or higher occurred in 3.8 percent of the patients. In no case was rigors recorded. In 4 of the patients studied (3%) post-biopsy urine cultures were infected with Escherichia coli. All post-biopsy blood cultures, both aerobic and anaerobic, were negative. Our data indicate that with the use of the double-glove technique, prophylactic administration of antibiotics is not necessary to prevent the infectious complications following transrectal biopsy of the prostate.
Natural rubber products, including surgical gloves, are known to be associated with contact dermatitis in allergic individuals. These cell-mediated immune reactions are usually not serious. However, an increasing number of reports have appeared recently in the medical literature concerning the association of latex with type I IgE-mediated anaphylactic reactions. We report on a patient with a history of cutaneous sensitivity to latex who experienced an intraoperative anaphylactic reaction felt to be secondary to latex surgical gloves. This report is intended to familiarize clinicians with this unexpected cause of intraoperative anaphylaxis.
In-vitro and in-vivo models for testing the antibacterial activity of glove materials are described. The models were tested using four types of material: latex containing residual accelerator, latex treated by solvent-extraction to remove accelerator, material from hypo-allergenic accelerator-free gloves, and latex coated with quaternary ammonium compounds. In-vitro tests revealed two distinct types of antibacterial activity, one related to the accelerator which was slow-acting, and not neutralizable, and a second faster-acting neutralizable activity due to the quaternary ammonium compounds. The antiseptic coated material also showed antibacterial activity on human skin.
In implant surgery air and surface contamination have become important factors in post-operative wound infection. We established the rate of contamination of surgical gown packs and found that dropping gloves onto the open gown pack prior to scrubbing had no effect on it. Ninety-six contact plates were used for this study, which was carried out during clean orthopaedic operations in one operating room. The overall rate of contamination was 65%. Most of the contaminants were skin commensals. The rate of contamination was 67% when gloves were dropped onto the gown pack compared with 63% when opened and presented to the scrubbed and gowned theatre staff. However this difference was statistically not significant. The high rate of contamination was probably due to the gown packs having been left opened for too long. This delay arose because each gown pack had three gowns and would have been avoided if single gown packs had been used.
The technique of interdental wiring was compared with a small-plate osteosynthesis technique (SPO) in a prospective study of surgical glove perforations acquired during the treatment of mandibular fractures. Using the SPO technique there was a significant reduction in the incidence of skin penetrating injuries in the surgeon (P less than 0.005) and assistant surgeon groups (P less than 0.05). The reduction in the incidence of glove perforation in the assistant surgeon group was very highly significant in the SPO series (P less than 0.001). The reduction in the surgeon group was not significant. No difference was noted in the scrub nurse group. The small-plate osteosynthesis technique has the advantage of reducing the risk of intraoperative cross-infection transmitted by hand contamination or penetrating injury. The recommended precautions for preventing the transmission of blood-borne pathogens are reviewed.
Following implantation of 50-mg samples of a starch powder used for glove lubrication, or of talc or calcium carbonate mould-release agents for gloves, in the peritoneal cavity of rats, the treated animals were killed in groups of ten after 2, 4, 8, 13, 26 and 52 wk and were examined. Groups of sham-operated animals were used as controls. Within each treatment group the frequency of peritoneal adhesions remained constant throughout the study. Talc produced significantly more adhesions than the other treatments and caused a more severe granulomatous reaction, which persisted. Calcium carbonate and starch powder produced similar numbers of adhesions and in both cases the residues became invested by a thin fibrous capsule. Calcium carbonate appeared a safer material than talc.
Cotton and synthetic knit glove fabrics in combination with an in vitro skin model were used to examine the capability of fabric to decrease the dermal absorption of the organophosphorus insecticides azinphos-methyl, paraoxon, and malathion. Capability for inhibition of acetylcholinesterase was determined in samples of media taken from under the skin barrier after the skin model, with or without fabric protection, had been exposed to the test compounds for 4 h. Acetylcholinesterase inhibitions caused by the direct addition of organophosphorus insecticide to the media were also included in the comparison. Results indicated that the skin model system alone had some capability to serve as a barrier to the transfer of organophosphates. Fabric covering used on the test model increased the barrier between insecticide application and resultant acetylcholinesterase inhibition. The all-cotton, 7-cut knit was especially effective in preventing the absorption of azinphos-methyl, as this organophosphorus insecticide had no capability to cause acetylcholinesterase inhibition when this fabric was used to protect the skin model. Knit glove materials of 100% cotton were demonstrated to be effective in preventing the absorption of paraoxon and malathion. These studies indicate that an in vitro model system can be used in combination with fabrics to study the relationship between clothing and skin as barriers to the absorption of organophosphorus insecticides.
Some of the physical factors considered to influence tactile perception with disposable, non-sterile gloves have been investigated. While it is apparent that tactile perception is influenced by a number of factors, the results of the present work indicate an inverse relationship between dentists' assessments of tactile awareness and stiffness measurements of the glove material.
BACKGROUND: Hospital-acquired infection often occurs because of lapses in accepted standards of practice on the part of health care personnel. The aim of this study is to attract attention on poor hospital infection control practice in venepuncture and use of tourniquets and emphasize the importance of hand hygiene. METHODS: Overall compliance with hygiene during usage of tourniquets and routine patient care before and after implementation of a hospital infection control measures was evaluated. RESULTS: According to the questionnaire, only 26.9% of respondents always washed their hands both before and after venepuncture. In the second step of the study, based on direct observation, hands were washed both before and after venepuncture on only 41 (45.1%) occasions. Failure to remove gloves after patient contact was observed on 23.1% occasions. CONCLUSION: Our survey reveals poor infection control practice in hand hygiene, glove utilization, and usage of tourniquets and the implementation of infection control measures produced a moderate improvement in compliance with them.
A wearable finger flexion monitor developed to measure hand function in individuals with hand dysfunction was evaluated for feasibility, measurement repeatability and reliability, fidelity of wireless transmission, and user acceptance. Configuration of the monitor allows use in situations when a traditional measurement glove cannot be worn. Five healthy individuals participated in the study of repeatability, while 10 healthy individuals and 10 individuals with acquired brain injury participated in trials to assess feasibility and user comfort. Repeatability results showed an overall error of 3.4 degrees , compared to 5.5 degrees and 5.7 degrees reported with other sensor gloves, and to manual measurements (5-8 degrees). Intraclass coefficient of reliability (using coefficient alpha) averaged 0.95. User feedback regarding comfort of the monitor was very high. Loss of data during wireless transmission was no greater than 1.2%. Results demonstrate that the monitor has a strong potential to be used as a tool for objective hand function evaluation in the home and community for both short- and long-term monitoring.
Beginning in the 1980s, use of latex gloves to protect health care workers against exposure to blood and body fluids increased. Since then, the number of reported cases of latex sensitivity also has increased. Reactions to latex range from contact dermatitis to anaphylactic shock. Low-powder, powder-free, and non-latex gloves provide alternatives to protect health care workers from occupational latex exposure.
Even with the most painstaking techniques, cornstarch granules used as surgical glove powder are deposited in the abdominal cavity during laparotomy. In tubal reconstructive surgery, aimed at the elimination of fibrous adhesions, this deposition may have undesirable consequences. After a successful operation, starch particles introduced in the abdominal cavity may result in the formation of new adhesions. It is therefore desirable to develop an alternative, completely nonreactive, glove powder.
This is a laboratory investigation on the emissions from batch combustion of representative infectious ("red bag") medical waste components, such as medical examination latex gloves and sterile cotton pads. Plastics and cloth account for the majority of the red bag wastes by mass and, certainly, by volume. An electrically heated, horizontal muffle furnace was used for batch combustion of small quantities of shredded fuels (0.5-1.5 g) at a gas temperature of approximately 1000 degrees C. The residence time of the post-combustion gases in the furnace was approximately 1 s. At the exit of the furnace, the following emissions were measured: CO, CO2, NOx, particulates and polynuclear aromatic compounds (PACs). The first three gaseous emissions were measured with continuous gas analyzers. Soot and PAC emissions were simultaneously measured by passing the furnace effluent through a filter (to collect condensed-phase PACs) and a bed of XAD-4 adsorbent (to capture gaseous-phase PACs). Analysis involved soxhlet extraction, followed by gas chromatography-mass spectrometry (GC-MS). Results were contrasted with previously measured emissions from batch combustion of pulverized coal and tire-derived fuel (TDF) under similar conditions. Results showed that the particulate soot) and cumulative PAC emissions from batch combustion of latex gloves were more than an order of magnitude higher than those from cotton pads. The following values are indicative of the relative trends (but not necessarily absolute values) in emission yields: 26% of the mass of the latex was converted to soot, 11% of which was condensed PAC. Only 2% of the mass of cotton pads was converted to soot, and only 3% of the weight of that soot was condensed PAC. The PAC yields from latex were comparable to those from TDF. The PAC yields from cotton were higher than those from coal. A notable exception to this trend was that the three-ring gas-phase PAC yields from cotton were more significant than those from latex. Emission yields of CO and CO2 from batch combustion of cotton were, respectively, comparable and higher than those from latex, despite the fact that the carbon content of cotton was half that of latex. This is indicative of the more effective combustion of cotton. Nearly all of the mass of carbon of cotton gasified to CO and CO2 while only small fractions of the carbon in latex were converted to CO2 and CO (20% and 10%, respectively). Yields of NOx from batch combustions of latex and cotton accounted for 15% and 12%, respectively, of the mass of fuel nitrogen indicating that more fuel nitrogen was converted to NOx in the former case, possibly due to higher flame temperatures. No SO2 emissions were detected, indicating that during the fuel-rich combustion of latex, its sulfur content was converted to other compounds (such as H2S) or remained in the soot.
The effects of surgical glove powder on adhesion formation after surgery on the flexor tendons in the chicken was analysed biomechanically. Surgical glove powder did not increase adhesion formation in flexor tendon surgery.