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Clinical evaluation of pesticide exposure and poisonings.

Pesticide exposures cause disorders varying from straightforward topical irritant reactions, such as those to synthetic pyrethroid insecticides, to complex systemic illness, such as that resulting from cholinesterase inhibition by organophosphate pesticides. The acute illness syndromes associated with pesticides most commonly encountered by clinicians are illustrated here by cases reported to the California Pesticide Illness Surveillance Program. The issues raised include asthma associated with exposure to contaminants in organophosphate insecticides, systemic toxicity of ingested pyrethroids (in children), and illnesses associated with spills or misuse of fumigants.

Carbamates↗

Neurobehavioral effects of pesticides: state of the art.

The authors have reviewed the literature on neurobehavioral toxicity of pesticides to assess the status of knowledge on this matter. Some data suggest that exposure to DDT and fumigants may be associated with permanent decline in neurobehavioral functioning and increase in psychiatric symptoms, but, due to the limited number of studies available and the scarce knowledge on exposure levels, no firm conclusion can be drawn. Data on subjects acutely poisoned with organophosphorous compounds suggest that an impairment in neurobehavioral performance and, in some cases, emotional status may be observed as a long-term sequela, but the possibility still remains that these effects were only an aspecific expression of damage and not of direct neurotoxicity. Studies carried out on subjects chronically exposed to organophosphates, but never acutely poisoned, do not provide univocal results but the slight changes consistently observed in sheep dippers suggest the need of focusing on activities characterized by relatively higher exposure levels. In general, the main limits of existing knowledge are the variability of the testing methods used, which makes it difficult to compare the results of single studies, and the scarce knowledge on exposure levels. A promising approach may be the conduction of prospective longitudinal or cohort studies, where exposure and dose assessment can be more easily controlled, or the evaluation of cohorts of workers a priori selected for the availability of environmental and biological monitoring data. The follow up of the populations under study may give an answer at the problem of the prognostic significance of the observed changes. Also the protocols used to assess neurobehavioral functioning need to be standardized.

Animals↗

Molecular cloning and characterization of the gene encoding glutathione reductase in Brassica campestris.

We have isolated the Brassica campestris cDNA encoding glutathione reductase of 502 amino acid residues with molecular mass of 54.5 kDa. The deduced amino acid sequences were 92.2%, and 79.5% identical to those of Arabidopsis thaliana, and pea, respectively. As expected, it exhibited a high degree of conservation within the region responsible for the redox reaction and for the binding of GSSG or NADPH. The gene was highly inducible by ozone fumigation or by paraquat treatment.

Amino Acid Sequence↗

Irradiation as a method for decontaminating food. A review.

Despite substantial efforts in avoidance of contamination, an upward trend in the number of outbreaks of foodborne illnesses caused by nonsporeforming pathogenic bacteria are reported in many countries. Good hygienic practices can reduce the level of contamination but the most important pathogens cannot presently be eliminated from most farms nor is it possible to eliminate them by primary processing, particularly from those foods which are sold raw. Several decontamination methods exist but the most versatile treatment among them is the processing with ionizing radiation. Decontamination of food by ionizing radiation is a safe, efficient, environmentally clean and energy efficient process. Irradiation is particularly valuable as an endproduct decontamination procedure. Radiation treatment at doses of 2-7 kGy--depending on condition of irradiation and the food--can effectively eliminate potentially pathogenic nonsporeforming bacteria including both long-time recognized pathogens such as Salmonella and Staphylococcus aureus as well as emerging or "new" pathogens such as Campylobacter, Listeria monocytogenes or Escherichia coli O157:H7 from suspected food products without affecting sensory, nutritional and technical qualities. Candidates of radiation decontamination are mainly poultry and red meat, egg products, and fishery products. It is a unique feature of radiation decontamination that it can also be performed when the food is in a frozen state. With today's demand for high-quality convenience foods, irradiation in combination with other processes holds a promise for enhancing the safety of many minimally processed foods. Radiation decontamination of dry ingredients, herbs and enzyme preparations with doses of 3-10 kGy proved to be a viable alternative to fumigation with microbicidal gases. Radiation treatment at doses of 0.15-0.7 kGy under specific conditions appears to be feasible also for control of many foodborne parasites, thereby making infested foods safe for human consumption. Microorganisms surviving low- and medium-dose radiation treatment are more sensitive to environmental stresses or subsequent food processing treatments than the microflora of unirradiated products. Radiation treatment is an emerging technology in an increasing number of countries and more-and-more clearances on radiation decontaminated foods are issued or expected to be granted in the near future.

Animals↗

Redox state and peroxidase system in sunflower plants exposed to ozone.

Sunflower plants subjected to a short-term fumigation with O(3) (150 ppb for 4 h repeated for 4 days) exhibited an increase in total ascorbate content, accompanied by a marked oxidation of ascorbate, leading to a decrease in its redox state, either at intracellular or extracellular level. O(3) exposure induced a rise in free extracellular peroxidase (POD) activity, assayed by syringaldazine as electron donor, as well as in the ionically and covalently cell wall bound PODs. On the contrary, the activity of both extracellular and intracellular guaiacol-POD did not show significant changes as a consequence of the pollutant exposure. The stimulation of syringaldazine-POD activities may be related to the effect of ozone on the growth of the cells, inducing an early senescence through the activation or acceleration of lignification processes. Beside, the reduced plasticity of the cell wall may oppose an unspecific mechanical resistance against the abiotic stress induced by the ozone exposure.

Journal Article↗

Carpet beetle dermatitis.

A 22-year-old man presented with a 5-year history of recurrent urticarial and papulovesicular lesions. Careful history suggested a close examination of his environment. The woolen rug in his bedroom was infested with larvae of a carpet beetle. The insect was identified as Anthrenus verbasci. Fumigation of the house with sulfuryl fluoride (Vikane) resulted in complete clinical recovery. Intradermal injection of the antigenic extract from the larvae showed a wheal and erythema on the patient's skin, and not on normal controls. This suggests that the patient had immediate-type hypersensitivity to some of the antigens extracted from larvae. Electron microscopic pictures of the insect are presented.

Adult↗

A new flux-orientated concept to derive critical levels for ozone to protect vegetation.

The current European critical levels for ozone (O3) to protect crops, natural and semi-natural vegetation and forest trees are based on a relative small number of open-top chamber experiments with a very limited number of plant species. Therefore, the working group "Effects of Ozone on Plants" of the Commission on Air Pollution Prevention of the Association of German Engineers and the German Institute of Standardization reanalysed the literature on O3 effects on European plant species published between 1989 and 1999. An exposure-response relationship for wild plant species and agricultural crops could be derived from 30 experiments with more than 30 species and 90 data points; the relationship for conifer and deciduous trees is based on 20 experiments with nine species and 50 data points. From these relationships maximum O3 concentrations for different risk stages are deduced, below which the vegetation type is protected on the basis of the respective criteria. Because it is assumed that the fumigation concentrations reflect the O3 concentrations at the top of the canopy, i.e. the upper surface boundary of the quasi-laminar layer if the micrometeorological big-leaf approach is applied, the application of these maximum O3 concentrations requires the transformation of O3 concentrations measured at a reference height above the canopy to the effective phytotoxic concentrations at the top of the canopy. Thus, the approach described in this paper is a synthesis of the classical concept of toxicology of air pollutants (critical concentrations) and the more toxicological relevant dose concept.

Crops, Agricultural↗

Effects of vapours of chlorpropham and ethofumesate on wild plant species.

Effects of vapours of two herbicides on plantlets of fourteen wild higher plant species and two bryophytes were screened in fumigation experiments using foliar injury, chlorophyll fluorescence and growth as response parameters. After vaporisation of the herbicides for 48 h, concentrations in the chambers reached 77 micrograms m-3 in the chlorpropham treatments and 184 ng m-3 in the ethofumesate treatments. Despite the higher concentrations of the volatile chlorpropham (vapour pressure, VP: 1.3 mP), plants showed no foliar injury, but vapours of this herbicide caused leaf crinkling in the agriophyte Agrostemma githago. The less volatile ethofumesate (VP: 0.56 mP) caused foliar injury in all higher species, with lowest no observed effect concentrations (NOECs) of 75 ng m-3. Chlorpropham affected growth only in Agrostemma, while ethofumesate reduced growth in one third of the higher plant species. Chlorophyll fluorescence proved to be a less suitable response parameter compared to foliar injury and growth. No adverse effects were observed in mosses, probably due to the slow growth and hence small doses of herbicides taken up. The extent of foliar injury due to ethofumesate showed a weak positive relationship to relative growth rates and specific leaf area in the tested higher plant species.

Benzofurans↗

The ability of apoplastic ascorbate to protect poplar leaves against ambient ozone concentrations: a quantitative approach.

Shoots of a sensitive (Populus nigra 'Brandaris') and a more tolerant (Populus euramericana 'Robusta') poplar clones were exposed for 30 days to Filtered Air or ambient O3-concentrations in fumigation cabinets. At regular intervals were determined: gas exchange of the leaves, the internal air space (Vair) and apoplastic water volume (Vapo) and the reduced (ASA) and oxidized (DHA) ascorbate concentration in the apoplast and in the mesophyll cells. The apoplastic ASA-concentration was 0.2 mM at the start of the experiment for both cultivars, while the effective cell wall thickness, estimated from Vapo, varied from 0.3 to 0.6 micron. Model calculations revealed that only 30% of the O3 molecules entering the apoplast was intercepted at these values. The O3-treatment induced a decline in stomatal conductance, an increase in Vapo and in the apoplastic ASA-concentration. As a result the estimated O3-flux to the cell membrane strongly declined. However, these responses occurred after the O3-induced reduction in photosynthesis. Moreover, they did not prevent early senescence of the leaves at a prolonged exposure. Therefore, it is concluded that the increase in apoplastic ASA-concentration was rather a general stress reaction of the affected poplar leaf than a (specific) defence reaction induced by O3. Our results suggest that other factors than the scavenging efficiency of apoplastic ASA were responsible for the difference in O3 sensitivity between both poplar cultivars.

Adaptation, Physiological↗

Limitations and perspectives about scaling ozone impacts in trees.

We review the need for scaling effects of ozone (O3) from juvenile to mature forest trees, identify the knowledge presently available, and discuss limitations in scaling efforts. Recent findings on O3/soil nutrient and O3/CO2 interactions from controlled experiments suggest consistent scaling patterns for physiological responses of individual leaves to whole-plant growth, carbon allocation, and water use efficiency of juvenile trees. These findings on juvenile trees are used to develop hypotheses that are relevant to scaling O3 effects to mature trees, and these hypotheses are examined with respect to existing research on differences in response to O3 between juvenile and mature trees. Scaling patterns of leaf-level physiological response to O3 have not been consistent in previous comparisons between juvenile and mature trees. We review and synthesize current understanding of factors that may cause such inconsistent scaling patterns, including tree-size related changes in environment, stomatal conductance, O3 uptake and exposure. carbon allocation to defense, repair, and compensation mechanisms, and leaf production phenology. These factors should be considered in efforts to scale O3 responses during tree ontogeny. Free-air O3 fumigation experiments of forest canopies allow direct assessments of O3 impacts on physiological processes of mature trees, and provide the opportunity to test current hypotheses about ontogenetic variation in O3 sensitivity by comparing O3 responses across tree-internal scales and ontogeny.

Air Pollutants↗

Consequences of elevated carbon dioxide and ozone for foliar chemical composition and dynamics in trembling aspen (Populus tremuloides) and paper birch (Betula papyrifera).

Atmospheric chemical composition affects foliar chemical composition, which in turn influences the dynamics of both herbivory and decomposition in ecosystems. We assessed the independent and interactive effects of CO2 and O3 fumigation on foliar chemistry of quaking aspen (Populus tremuloides) and paper birch (Betula papyrifera) at a Free-Air CO2 Enrichment (FACE) facility in northern Wisconsin. Leaf samples were collected at five time periods during a single growing season, and analyzed for nitrogen. starch and condensed tannin concentrations, nitrogen resorption efficiencies (NREs), and C:N ratios. Enriched CO2 reduced foliar nitrogen concentrations in aspen and birch; O3 only marginally reduced nitrogen concentrations. NREs were unaffected by pollution treatment in aspen, declined with 03 exposure in birch, and this decline was ameliorated by enriched CO2. C:N ratios of abscised leaves increased in response to enriched CO2 in both tree species. O3 did not significantly alter C:N ratios in aspen, although values tended to be higher in + CO2 + O3 leaves. For birch, O3 decreased C:N ratios under ambient CO2 and increased C:N ratios under elevated CO2. Thus, under the combined pollutants, the C:N ratios of both aspen and birch leaves were elevated above the averaged responses to the individual and independent trace gas treatments. Starch concentrations were largely unresponsive to CO2 and O3 treatments in aspen. but increased in response to elevated CO2 in birch. Levels of condensed tannins were negligibly affected by CO2 and O3 treatments in aspen, but increased in response to enriched CO2 in birch. Results from this work suggest that changes in foliar chemical composition elicited by enriched CO2 are likely to impact herbivory and decomposition, whereas the effects of O3 are likely to be minor, except in cases where they influence plant response to CO2.

Betula↗

Physiological and biochemical responses of two cultivars of wheat to elevated levels of CO2 and SO2, singly and in combination.

In view of the present increasing trends of anthropogenic emissions of carbon dioxide (CO2) and sulphur dioxide (SO2) throughout the world, the present study was aimed at investigating the long-term influence of elevated concentrations of CO2 and SO2, singly and in combination on the physiological and biochemical characteristics of two cultivars of wheat (Triticum aestivum L. cv. Malviya 234 and HP1209). For this purpose, the plants were grown in open top chambers under field conditions and were fumigated with 600 ppm CO2, 0.06 ppm SO2 and 600 ppm CO2 + 0.06 ppm SO2 separately for 8 h daily (0800-1600 h) from germination to grain maturity. The individual treatment of SO2 advers#ely affected both the cultivars of wheat by reducing protein and starch contents. The respiration rate, total soluble sugars and total phenolics, however, increased in response to SO2. Stimulation of photosynthesis rate and reduction in stomatal conductance and transpiration rate were observed under CO2 treatment. Concentrations of total soluble sugars, starch and total phenolics increased in response to CO2 and CO2 + SO2 treatments. In combined treatment, CO2 modified the plant response to SO2 in both the cultivars. Elevated CO2 increased the photosynthesis rate under combined treatment. Higher levels of starch and soluble sugars under combined treatment provided extra carbon for SO2 detoxification. The pattern of intraspecific response of wheat to different treatments was more or less similar, but the magnitude of response differed significantly.

Air Pollutants↗

Ozone differentially affects physiological and biochemical responses of two clover species; Trifolium repens and Trifolium pratense.

The effect of acute ozone exposure (150 ppb for 3 h) on two clover species, white clover (Trifolium repens L.) and red clover (Trifolium pratense L.) was investigated through the analysis of 10 different physiological and biochemical parameters. Twenty-four hours after fumigation, visible symptoms of injury on leaves were observed only in red clover, but from the biochemical point of view, both species revealed significant ozone-induced modifications. A decrease in the photosynthetic efficiency as well as an increase in the de-epoxidation index and a decrease in the redox state of ascorbate were detected only in T. pratense leaves; no significant change in pigment content was found in either of the two species. On the other hand, both white and red clover showed, although to different extents, significant decreases in the activities of the antioxidant enzymes peroxidase and ascorbate peroxidase. Multivariate statistical analysis revealed not only that ozone affects both species, but also that they differentially respond to the pollutant, confirming the higher sensitivity of Trifolium pratense to ozone exposure.

Analysis of Variance↗

The response of some common Egyptian plants to ozone and their use as biomonitors.

Relative sensitivity of five common Egyptian plant species namely, Senecio vulgaris, Malva parviflora, Sonchus oleraceus, Medicago sativa and Melilotus indicus to elevated levels of ozone has been studied. The plants were exposed to charcoal filtered air (CFA) and different levels of O3 (50 and 100 ppb) for 5 h per day. The studied parameters were recorded for five consecutive days after fumigation. The foliar injury varied significantly among species in a dose-dependent manner. Severe injury symptoms were recorded on the leaves of M. sativa. With the exception of M. parviflora, all species exhibited significant increases in the percentage reduction of the above-ground dry weight as a result of reductions in both leaf and stem dry weights. M. sativa showed a marked reduction in its relative growth rate at elevated levels of O3. The extent of chlorophyll a destruction was higher in both M. sativa and S. oleraceus than in the other species tested. No differences in the sensitivity of chlorophylls a+b and carotenoids to ozone levels were recorded in this work. Percentage reduction of ascorbic acid was higher in M. sativa and S. oleraceus, compared with the other species studied. With respect to relative percentages of proline, there was a significant difference in the responses of plants to ozone. According to the ozone resistance (R%), measured as relative growth rate, the test species were arranged in the descending order: M. parviflora>M. Indicus>S. Vulgaris>S. Oleraceus>M. sativa. In M. sativa, both determinant and correlation coefficients are well reflected in the relationship between its physiological response, its performance and ozone levels, supporting its recommendation as a candidate for biomonitoring in Egypt.

Air Pollutants↗

From critical levels to critical loads for ozone: a discussion of a new experimental and modelling approach for establishing flux-response relationships for agricultural crops and native plant species.

Present critical levels for ozone (O3) for protecting vegetation against adverse effects are based on exposure-response relationships mainly derived from open-top chamber experiments and are expressed as an Accumulated exposure Over a Threshold of 40 ppb (AOT40). In that context with a revision of the UN (United Nations)-ECE (Economic Commission for Europe) Gothenburg protocol, AOT40 values should be replaced by flux-oriented quantities, i.e. in the end by critical loads. At present, the database for the derivation of critical loads for O3 is extremely inadequate. Furthermore, the currently available flux-response relationships are also derived from open-top chamber experiments. The use of a relationship for spring wheat in a risk assessment for an agricultural site in Hesse, Germany, demonstrates in principle, the applicability of the critical load concept for O3. Comparisons of diurnal variation of stomatal uptake and AOT40 showed that a major part of toxicologically effective stomatal uptake occurred before noon whereas the AOT40 values were dominated by the O3 concentrations during afternoon. In other words, the AOT40 exposure index do not adequately address the O3 burden during hours when plants are sensitive to O3 uptake. However, due to the differences in radiation, air temperature and humidity between the chamber and the ambient microclimates, a derivation of flux-response relationships from chamber experiments is likely to be questionable, especially for species rich ecosystems: Here, without any changes in the pollution climate, significant modifications of species composition as well as an earlier beginning of the growing season has been previously observed. To overcome the problems associated with chamber-derived flux-response relationships, a new experimental and modelling concept, was developed. The approach, briefly described in this paper, combines methods in air pollution toxicology and micrometeorology. As an analogy to the free-air fumigation concept, O3 is released into the air by an injection system above the plant canopy. The assessment of dispersion and surface deposition of O3 released is based on Lagrangian trajectory modelling. Depending on wind direction and velocity, atmospheric stratification and surface roughness, without any disturbance of the microclimate and micrometeorology, several sub-areas can be identified around the source position with differing deposition rates above the ambient level. Taking into account the actual O3 background deposition, deposition rates and vegetation responses observed in these sub-areas can easily be used to derive flux-effect relationships under ambient conditions and more realistic limiting values to protect our environment.

Air Pollutants↗

Effects of dietary and gaseous fluoride on the aphid Aphis fabae.

A large proportion of leaf fluoride consists of surface deposits and a sucking herbivore would be expected to take in a smaller load of fluoride in its food than a chewing herbivore. In these experiments, fluoride was applied aerially, through the leaves, and systemically, via the roots, to compare uptake by aphids and effects on their fecundity. Fluoride applied via roots was taken up by both the plants and the aphids, but at high treatment rates the aphids had much lower concentrations than the foliage. When the plants and aphids were fumigated with HF the aphids had much greater loads than the plant shoots, which was due to deposition of F on the insect surfaces. There were no effects of the treatments on aphid reproduction or development time. The aphids obtained some fluoride through their diet which suggests that fluoride is present in the phloem sap, previously thought to be of minor importance.

Journal Article↗

Growth variation of white oak subjected to historic levels of fluctuating air pollution.

Growth of white oak (Quercus alba L.) trees was examined, using tree-ring analysis, at three sites near a small, remote coal-fired power plant in central Pennsylvania, USA. Forests immediately adjacent to the power plant have been subjected to power plant emissions since the power plant initiated operation in 1954. However, localized, ground-level fumigations have been gradually reduced over the years due to a series of construction projects resulting in increased stack heights. Comparisons of growth were made among the white oaks growing at the three close-in sites, as well to the growth of white oak at three control sites located 10-50 km from the power plant, during periods of differing stack heights. White oak exhibited reduced growth at two of the close-in sites during the time period when historical ground-level air pollution exposures were assumed to be greatest due to low stack heights. White oak growth at the third close-in site was not substantially reduced during this time period. In 1976, taller stacks were implemented at the power plant to reduce local, ground-level concentrations of air pollutants. The recovery of tree growth at the two close-in affected sites, and increased synchronous growth responses from 1976-85 among all three close-in sites, indicates that implementation of taller stacks in 1976 reduced ground-level pollutant levels to such dosages that growth was not impaired at any site. Also, growth rates after 1976 were comparable to the growth rates of the white oaks growing on the control sites. A possible interacting factor was a severe drought that occurred in the mid-1960s in central Pennsylvania.

Journal Article↗

Responses of spruce seedlings (Picea abies) to exhaust gas under laboratory conditions--II. Ultrastructural changes and stomatal behaviour.

This study examines the effects of exhaust gas exposure on the epistomatal wax structure and mesophyll ultrastructure in needles of Norway spruce (Picea abies (L.) Karst.) seedlings. Stomatal diffusive resistance was also measured. Two independent exhaust gas fumigations were performed: 100 and 200 ppb measured as NO(x), for 10 days and 50, 100 and 200 ppb NO(x) for 19 days. The obstructive effect of exhaust gas exposure on epistomatal wax tubules was apparent. The stomata became covered by flat and solid wax resulting from the structural degradation of the wax crystalloids. Increasing the exhaust gas concentration in the chamber atmosphere exacerbated the degradation of the wax structure. Exhaust gas exposure induced aggregation and electron translucence of plastoglobuli, swelling of thylakoids, increase of cytoplasmic lipids and slight increase of vesiculation of cytoplasm in mesophyll cells of current and previous year needles. These changes were exemplified in current year needles. Damage to the epicuticular waxes and mesophyll ultrastructure of spruce needles most likely reflects the NO(x) and volatile hydrocarbon fraction. The alterations in epicuticular waxes and mesophyll ultrastructure can be related to accelerated senescence of the youngest, photosynthetically active, needle generation. The exhaust gas also resulted in decreased diffusive stomatal resistance at night which indicates that the exhaust gas exposure disturbed the gas exchange of spruce seedlings. The results show that even relatively short-term exposure to realistic concentrations of exhaust gas in the atmosphere can induce rather severe injuries to the needle surface structure as well as ultrastructure at the cellular level.

Journal Article↗