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PubMed2002

Mesophilic and thermophilic activated sludge post treatment of anaerobic effluent. Sludge and wastewater characterisation using batch experiments.

Anaerobic pretreated paper process water was characterized in terms of readily biodegradable, slowly biodegradable, very slowly biodegradable and inert wastewater fractions under mesophilic and thermophilic conditions. The anaerobic pretreated paper process water contained a relatively high amount of slowly biodegradable components and few easily biodegradable components as indicated by the ratio of short-term BOD over the BOD5. Wastewater readily biodegradable COD, determined as short-term BOD, was almost similar when measured under both temperature conditions. Fractions of slowly biodegradable COD and inert COD of the same wastewater were found to depend on the type of biomass involved in the test. Thermophilic aerobic biomass was not able to degrade the wastewater to the same extent as the mesophilic biomass resulting in higher apparent inert COD levels. Furthermore, wastewater colloidal COD did not flocculate under thermophilic conditions and was thus not removed from the liquid phase.

Aerobiosis↗
PubMed2002

Use of bituminous coal for concentration of enteroviruses from sewage and effluent.

A method has been developed for concentration of enteroviruses from untreated and treated domestic wastewater using bituminous coal bed as a virus adsorbent. A bed made from 1.5 g of 120 mesh coal powder was used for concentrating enteroviruses from 100 ml of clarified sewage at different pH values with and without addition of AlCl3. To enhance the adsorption of viruses, requisite quantities of aluminium chloride were added so that a final concentration of 0.0005 M could be achieved. At pH 3.0 maximum adsorption (82.8%) of poliovirus type 1 from artificially contaminated clarified sewage was observed without addition of AlCl3. However, at pH 5.0 maximum virus adsorption of 98.7% was achieved after addition of aluminium chloride. An average recovery of 86.9% of adsorbed viruses at pH 5.0 was achieved from coal bed with 3% flocculating beef extract at pH 9.5. This method for concentration of enteroviruses incorporating use of coal was compared with that of Millipore membrane filter method applied to raw sewage and clarified sewage. The results obtained from the methodology using coal as adsorbent was subjected to Student's "t" test and it was observed that its efficiency is confirmed for recovery of enteroviruses from raw and nonclarified sewage. These results are also comparable with that obtained with MF method. The results presented in this paper are indicative of the potential of this method for both treated and raw sewage.

Adsorption↗
PubMed1989

An improved latex agglutination-inhibition test for the detection of human chorionic gonadotropin in urine.

A commercially available slide latex agglutination-inhibition test for pregnancy was used in a tube modification for the detection of human chorionic gonadotropin (hCG) in urine. The tube method used 100 microliters sample and small quantities of reagents which were mixed continuously for 30 min. The results obtained, based on flocculation, were 15-30-fold better than those obtained by slide agglutination using spiked samples or urine samples from pregnant women. The lowest concentration of hCG detected in the spiked samples was 31 IU/l. When 43 clinical specimens were examined, the tube method detected four more samples than the 18 detected by the slide method. A good correlation (r = 0.96) was observed between the two methods in the determination of the hCG content in the positive samples.

Chorionic Gonadotropin↗
PubMed1995

Potential usefulness of bacteriophages that infect Bacteroides fragilis as model organisms for monitoring virus removal in drinking water treatment plants.

The presence of bacteriophages at different stages in three drinking water treatment plants was evaluated to study the usefulness of phages as model organisms for assessing the efficiency of the processes. The bacteriophages tested were somatic coliphages, F-specific coliphages, and phages infecting Bacteroides fragilis. The presence of enteroviruses and currently used bacterial indicators was also determined. Most bacteriophages were removed during the prechlorination-flocculation-sedimentation step. In these particular treatment plants, which include prechlorination, phages were, in general, more resistant to the treatment processes than present bacterial indicators, with the exception, in some cases, of clostridia. Bacteriophages infecting B. fragilis were found to be more resistant to water treatment than either somatic or F-specific coliphages or even clostridia. Enteric viruses were found only in untreated water in low numbers, and consequently, the efficiency of the plants in the removal of viruses could not be evaluated with precision. The numbers and frequencies of detection of the various microorganisms in water samples taken in the distribution network served by the three plants confirm the results found in the finished water at the plants.

Bacteriophages↗
PubMedDate not supplied

Evaluation of high-rate clarification for wet-weather-only treatment facilities.

High-rate clarification (HRC) processes are well suited for enhanced primary treatment of wet-weather flows in combined sewer systems. Suspended solids removal in excess of 75% of influent concentrations can be achieved consistently. Chemical oxygen demand and five-day biochemical oxygen demand removal are better than 60%. However, although optimal treatment could be achieved in a matter of minutes when the units were started full, a delay of up to one-half hour was observed when the units were started empty. Operational strategies that minimize this effect need to be developed in full-scale tests before HRC processes can be implemented with confidence in wet-weather-only facilities.

Filtration↗
PubMed2001

Modeling of a reactive primary clarifier.

Even though many models have been proposed for primary clarification, none is directly compatible with the ASM1. The objective of this paper is to present the development of a reactive primary clarifier model to be used in a wastewater treatment plant simulator (WEST). A model simulating COD behavior has been developed based on the Takacs model, and was tested with full-scale data. Particulate effluent COD was well described but problems occurred predicting the underflow suspended solids concentration. The model had to be upgraded with a residence time and a flocculation term to simulate the behavior of soluble COD. An ammonification term was added to the model, resulting in an improved model fit on effluent ammonium.

Belgium↗
PubMed2006

Karyomegaly and intranuclear inclusions in the renal tubules of sentinel ICR mice (mus musculus).

Among 585 sentinel ICR mice (Mus musculus), 8 (7 female, 1 male) had unusual microscopic lesions in the kidney. Light microscopy revealed occasional tubular epithelial cells with large, karyomegalic nuclei that contained intranuclear inclusions and marginated chromatin. These cells were randomly present in the cortex and medulla but were more prominent near the corticomedullary junc tion. Rare pyknotic cells and mild interstitial infiltrates of lymphocytes and plasma cells were associated with occasional foci of abnormal cells. Electron microscopy performed on 2 (1 female, 1 male) of the mice demonstrated intranuclear inclusions composed of abundant flocculent, electron-lucent material. No viral particles or other pathogens were identified. General health monitoring that included serology, microbiology, parasitology, necropsy, and histopathology was negative for pathogens. Polymerase chain reaction-based testing for polyomavirus and immunohistochemistry for adenovirus were performed on 5 of the 7 female mice; all were negative for both viruses. In light of microscopy findings and the lack of evidence for an infectious agent, the tubular lesions were considered degenerative changes, possibly due to a toxic insult. The cause and significance of the findings in these mice can not be explained fully.

Animals↗
PubMed2005

The flocculation performance of Tamarindus mucilage in relation to removal of vat and direct dyes.

A food grade natural mucilage, extracted from the seeds of Tamarindus indica pods, is used as a flocculant for removal of solubilised vat (golden yellow) and direct dye (direct fast scarlet) in aqueous solutions. The maximum removal obtained was 60% for golden yellow after 2 h and was 25% for direct fast scarlet after 1 h. The optimum mucilage dose was 10 mg/l and 15 mg/l for golden yellow and direct fast scarlet, respectively. The pH values also seem to affect the percent removal of both the dyes significantly. In case of vat dye, the pH value of the test samples affected the percent removal significantly. The change was highly significant between neutral and alkaline pH. In case of direct dye, there was no significant change in percent removal at pH 7 and pH 4 whereas a significant change in percent removal was observed between pH 7 and pH 9.2. The plausible mucilage-dye interaction and flocculation mechanism has been discussed. This new flocculant works better in the case of vat dye removal compared with the direct dye.

Adhesives↗
PubMed2006

The key enzyme in galactose metabolism, UDP-galactose-4-epimerase, affects cell-wall integrity and morphology in Candida albicans even in the absence of galactose.

The enzyme UDP-galactose-4-epimerase (GAL10) catalyzes a key step in galactose metabolism converting UDP-galactose to UDP-glucose which then can get metabolized through glycolysis and TCA cycle thus allowing the cell to use galactose as a carbon and energy source. As in many fungi, a functional homolog of GAL10 exists in Candida albicans. The domainal organization of the homologs from Saccharomyces cerevisiae and C. albicans show high degree of homology having both mutarotase and an epimerase domain. The former is responsible for the conversion of beta-d-galactose to alpha-d-galactose and the latter for epimerization of UDP-galactose to UDP-glucose. Absence of C. albicans GAL10 (CaGAL10) affects cell-wall organization, oxidative stress response, biofilm formation and filamentation. Cagal10 mutant cells tend to flocculate extensively as compared to the wild-type cells. The excessive filamentation in this mutant is reflected in its irregular and wrinkled colony morphology. Cagal10 strain is more susceptible to oxidative stress when tested in presence of H2O2. While the S. cerevisiae GAL10 (ScGAL10), essential for survival in the presence of galactose, has not been reported to have defects in the absence of galactose, the C. albicans homolog shows these phenotypes during growth in the absence of galactose. Thus a functional CaGal10 is required not only for galactose metabolism but also for normal hyphal morphogenesis, colony morphology, maintenance of cell-wall integrity and for resistance to oxidative stress even in the absence of galactose.

Amino Acid Sequence↗
PubMed2006

Colour removal from landfill leachate by coagulation and flocculation processes.

A study was conducted to investigate the efficiency of coagulation and flocculation processes for removing colour from a semi-aerobic landfill leachate from one of the landfill sites in Malaysia. Four types of coagulant namely aluminium (III) sulphate (alum), ferric (III) chloride, ferrous (II) sulphate and ferric (III) sulphate were studied using standard jar test apparatus. Results indicated that ferric chloride was superior to the other coagulants and removed 94% of colour at an optimum dose of 800 mg/l at pH 4. The effect of coagulant dosages on colour removal showed similar trend as for COD, turbidity and suspended solids. This suggested that colour in landfill leachate was mainly contributed by organic matters with some insoluble forms that exhibited turbidity and suspended solids readings. The results from this study suggested that ferric chloride could be a viable coagulant in managing colour problems associated with landfill leachate.

Color↗
PubMed2004

Biological nutrient removal from pre-treated landfill leachate in a sequencing batch reactor.

Biological treatment of landfill leachate usually results in low nutrient removals because of high chemical oxygen demand (COD), high ammonium-N content and the presence of toxic compounds such as heavy metals. Landfill leachate with high COD content was pre-treated by coagulation-flocculation with lime followed by air stripping of ammonia at pH=12. Nutrient removal from pre-treated leachate was carried out using a lab-scale sequencing batch reactor (SBR). Three different operations consisting of different numbers of steps were tested and their performances were compared. These operations were the three-step anaerobic (An)/anoxic (Ax)/oxic (Ox); the four-step (An/Ox/Ax/Ox), and the five-step (An/Ax/Ox/Ax/Ox) operations with total residence time of seven hours each. Experiments were carried out using three consecutive operations with a total cycle time of 21 h at a constant sludge age of 10 days. The lowest effluent nutrient levels were realized by using the five-step operation which resulted in effluent COD, NH4-N and PO4-P contents of 1,400, 107 and 65 mg l(-1), respectively, at the end of 21 h. Addition of domestic wastewater (1/1, v/v) and powdered activated carbon (PAC, 1 g l(-1)) to the pre-treated leachate improved nutrient removals in the five-step SBR operation, resulting in 75% COD, 44% NH4-N and 44% PO4-P removals after 21 hours of operation.

Bacteria, Anaerobic↗
PubMed1983

Loiasis in an American naturalist.

In March 1981, a 32-year-old male naturalist involved with collecting mammals for study was found to have an unexpected eosinophilia. Serum parasitic screening done at the Centers for Disease Control showed filarial titer by indirect hemagglutination of 1:1,024 and of 1:40 by bentonite flocculation. His travel history disclosed 3 months spent in Cameroon in 1978. He also gave a history of intermittent arm swelling for at least a year. Peripheral blood, collected repeatedly at mid-day and midnight and tested for microfilariae by the Knott technique, was negative. He was begun empirically on diethylcarbamazine, and on day 11 of treatment he removed a worm, identified as a male Loa loa, from his leg. He completed treatment without difficulty and has done well.

Adult↗
PubMed1985

[Sero- and immunodiagnosis in parapoxvirus infections in the human. Milker's nodes, ecthyma contagiosum contact infection].

The currently available methods for the serological and immunological diagnosis of human parapoxvirus infection (milker's nodule, farmyard pox) are demonstrated by the case of a man infected by contact with sheep carrying ecthyma contagiosum lesions. Compared to virus identification by electron microscopy, cell culture and animal experiments, identification of viral antigen in skin biopsies is equally sensitive during the first 2 weeks of the disease, whereas it is far more sensitive afterwards. The diagnosis of a parapoxvirus infection may be confirmed, even after skin lesions have healed, by assays for agglutinating, complement fixing, neutralizing and flocculating antibodies in patients' serum, the most sensitive method currently used being immunofluorescence and enzyme assays (ELISA). Additionally, antibodies on cell surfaces, which are regularly found in parapoxvirus infection, may be used to confirm the diagnosis by testing recall antigens. By means of serological assays used for routine purposes as well as by negative staining, the genus parapoxvirus can be identified, but not the species. Cross immunity between orthopoxvirus and parapoxvirus do not occur. The three species of the genus of parapoxvirus may be differentiated by DNA-hybridization techniques or by surface structure analysis using immunoelectron microscopy. According to clinical, histological, serological and electron microscopical features, the diseases caused by the three parapoxvirus species are identical in humans. The clinical entity in humans should be called farmyard pox regardless of the species of virus isolated.

Adult↗
PubMed1976

Agglutination with O antisera of salmonella exposed to antibiotics.

Five strains of Salmonella were grown on agar containing subinhibitory concentrations of ampicillin, gentamicin, tetracycline, or chloramphenicol, and on drug-free agar (control). Antigens were prepared by heating bacteria suspensions in saline solution at 100 C or by suspension in alcohol. Antigens were tested for agglutinability with somatic O antisera and fluorescent antibody staining. The alcohol-treated antigens prepared from organisms grown in the presence of antibiotics other than ampicillin had lower agglutination titers than did the control grown on drug-free agar. Heat-treated control antigens had lower agglutination titers than did the alcohol-treated control antigens. The agglutinability of heat-treated antigens prepared from organisms that had been exposed to antibiotics was preserved or enhanced. The agglutinated filaments produced a flocculent sediment, in contrast to the granular sediment of the controls. No significant morphologic abnormality in cell-wall structures of antibiotic-exposed cells could be detected by either electron microscopy or the fluorescent antibody staining.

Agglutination Tests↗
PubMed2003

Evaluation of Bacillus subtilis and coliphage MS2 as indicators of advanced water treatment efficiency.

The assessment of water treatment facilities for their efficiency using alternate indicators is of paramount importance. Current methods for assessing efficiency are limited by the specific characteristics of the microorganisms, such as their different sensitivities to disinfectants. A pilot study was carried out to compare different treatment scenarios for the future upgrade of the Sergio Cuevas Water Treatment plant (the largest in the Caribbean) in San Juan, Puerto Rico. The treatment units under investigation included a coagulation-flocculation-sedimentation unit, dual-media filters, micro-filtration units, intermediate ozone injection and contact columns as well as a biological filtration unit. The plant was challenged at different stages of treatment with Bacillus subtilis spores and MS2 coliphages in an attempt to test them as possible alternate indicators of treatment plant performance. These organisms were chosen because of their resistance to disinfection and desiccation, their low analysis costs and ease of detection. The removal of spores and coliphages by each treatment unit tested was calculated by seeding a known concentration (5-7 log10) of spores and coliphages and following the removal or disinfection rates. The seeded indicators were detected using traditional culture techniques. Ballasted clarification was shown to be highly efficient at removing 99.1% (approximately 3 log10) of the spores and 85.1% (approximately 0.86 log10) of MS2. Ozone treatment inactivated 80.37% (approximately 1.4 log10) spores and 99.95% (approximately 3.07 log10) coliphages. The coliphage inactivation rate obtained confirmed data obtained by previous studies indicating that MS2 was less resistant to ozonation than B subtilis spores. The membrane technology had the best efficiency in terms of physical removal of spores achieving over 99.9% (> 3 log10) removal. Coliphage removal mechanisms remain to be determined and will be a future focus of the study. Preliminary results indicate that aerobic spores and coliphages may be useful as indicators to determine the efficiency of different drinking water treatment technologies.

Bacillus subtilis↗
PubMed2001

Scaling Behavior of the Rheological Properties of Montmorillonite Suspensions: Correlation between Interparticle Interaction and Degree of Flocculation.

In this work we investigate some aspects of the rheological behavior of sodium montmorillonite (NaMt) suspensions in the pH range 3 to 9, of NaCl concentrations between 10(-3) and 10(-1) M, and of solid concentrations between 5 and 11% w/v. Three kinds of experiments were performed: steady-state viscometry, oscillatory test, and creep recovery. The physical quantities of interest were the yield stress sigma(y) of the suspensions, the elastic rigidity modulus G', and the instantaneous elastic compliance. Furthermore, G' was obtained from oscillatory tests in three different experiments: determination of the viscoelastic linear region, oscillograms, and the gelation process. All quantities were found to scale with the concentration of solids, C, according to a power law of the form Y=k(y)C(n). The exponents n were found to change from approximately 3 to approximately 6 when the pH was increased from 3 to 9 (at constant ionic strength 10(-2) M), although values as high as 10 were estimated when the NaCl concentration was reduced to 1 mM. Such values of n correlate well with the characteristics of the edge-to-face (E-F), edge-to-edge (E-E), and face-to-face (F-F) interparticle interactions. The minimum values of n correspond to maximum E-F attractions, whereas the largest n are associated with strong F-F repulsions. Copyright 2001 Academic Press.

Journal Article↗