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The biomechanics of hybrid external fixation.

OBJECTIVE: The objective of this study was to ascertain which factors determine the rigidity of a hybrid external fixation frame in a tibial periarticular fracture model. DESIGN: Laboratory investigation using a polyvinylchloride pipe periarticular tibia fracture model. SETTING: Simulated periarticular tibia fractures were created in a tibia model. Instrumented specimens were tested in a biomaterials testing system. INTERVENTION: Groups of simulated periarticular tibia fractures were stabilized with one of nine different external fixation constructs with components from one manufacturer. MAIN OUTCOME MEASUREMENTS: Elastic stiffness was measured for each specimen in compression, torsion, flexion bending, extension bending, and varus and valgus bending. RESULTS: The four-ring Ilizarov fixator was the stiffest in all modes of testing. There was a trend toward increasing stiffness with an increasing number of rings. Fixators constructed with multiple levels of fixation in the periarticular fragment were stiffer in all modes of testing. The additional spacing between wires gained by the use of a single thick carbon ring or the use of a drop wire three centimeters from a single ring did not increase stiffness in this model. Frame modifications intended to augment the bar-to-ring connection did not increase stiffness. The use of rings mounted with half-pins instead of a unilateral bar mounted with half-pins for diaphyseal fixation increased the stiffness of the frame only in torsional testing. CONCLUSIONS: Although the ideal stiffness of an external fixator is unknown, our results show that the addition of more than one level of fixation in the periarticular fragment increases the stiffness of hybrid external fixators in this periarticular tibia fracture model. Augmentation of the ring-to-bar connection did not significantly affect the stiffness of the frame.

Biomechanical Phenomena↗

Timing of femur fracture fixation: effect on outcome in patients with thoracic and head injuries.

BACKGROUND: Optimal timing of femur fracture fixation remains controversial. This study examines the association between the timing of femur fracture fixation and outcome in patients with concomitant chest and head injuries. METHODS: A retrospective review of registry data from a Level I trauma center identified 1362 patients with a femoral shaft fracture over a 12-year period. Patients were categorized into five groups by timing of femur fracture fixation: Group 1, within 24 hours; Group 2, 24 to 48 hours; Group 3, 48 to 120 hours; Group 4, > 120 hours; and Group 5, patients with no operative fixation. Primary outcome measures included morbidity (specifically, pulmonary complications) and mortality. Secondary outcome measures were hospital length of stay, intensive care unit length of stay, and discharge Glasgow Coma Scale score. Subsets of patients were examined including all patients with multiple injuries (Injury Severity Score > 15), chest trauma (Chest Abbreviated Injury Scale score > or = 2), and head trauma (Head Abbreviated Injury Scale score > or = 2). RESULTS: Acute respiratory distress syndrome, pneumonia, hospital length of stay, and intensive care unit length of stay were lowest in the group fixed within 24 hours, even in patients with concomitant head or chest trauma. Fixation between 2 and 5 days was associated with a significantly increased incidence of acute respiratory distress syndrome, pneumonia, and fat embolization syndrome in patients with concurrent chest trauma (p < 0.0001). In head-injured patients, discharge Glasgow Coma Scale score was highest in the group fixed within 24 hours. Timing of operative fixation did not affect mortality. CONCLUSION: Our data show that early femur fracture fixation (< 24 hours) is associated with an improved outcome, even in patients with coexistent head and/or chest trauma. Fixation of femur fractures at 2 to 5 days was associated with a significant increase in pulmonary complications, particularly with concomitant head or chest trauma, and length of stay. Chest and head trauma are not contraindications to early fixation with reamed intramedullary nailing.

Adult↗

Biomechanics of lumbar pedicle screw/plate fixation in trauma.

This investigation was conducted to determine alterations in the biomechanical strength and stiffness characteristics of the lumbar spine fixated with Steffee instrumentation. Comparative studies of these parameters were conducted using seven lumbar columns from fresh human cadavers. Three runs were conducted on each T12-L5 column: control, injured, and fixated. The specimens were loaded under the compression-flexion mode until failure (control run) and then reloaded (injury run) to the failure deformation determined in the control run. Screw/plates were then inserted one level proximal and distal to injury, and the specimens were reloaded (fixation run). Radiographs were taken before and after each trial. Data on deformation and force histories were gathered. The load-deflection response of the injured and fixated specimens were bimodal with two representative stiffnesses. Control failure loads and stiffnesses were higher than those for the injured (P less than 0.001) or fixated (P less than 0.01) spine. Initial stiffness was significantly higher for the fixated than for injured columns (P less than 0.001), but the final stiffnesses were similar. The increase in the initial stiffness in the fixated specimen compared to the injured specimen indicates the strength added to the posterior region of the spine. The relatively smaller alteration in the final stiffness between the fixated and the injured columns, corresponding to the load shared by the anterior column, may suggest that, above a critical strain level, the anterior column absorbs a higher portion of the external load and posterior fixation may be inadequate as sole treatment in trauma.

Adult↗

Stability of oculomotor fixation as a function of target contrast and blur.

Previous studies have shown that changes in target parameters, such as luminance, color, size, and shape exert little effect on the stability of fixation, measured as the standard deviation of eye positions over several seconds of fixation. In this study, we investigated the effect of target contrast and dioptric blur on the stability of fixation in five normal subjects. The horizontal positions of both eyes were assessed by infrared limbal tracking while the subjects fixated binocularly for 20-s intervals on a cross-shaped target at 2 m. Across subjects, the mean stability of fixation on a high contrast target worsened significantly from 8.9 to 12.0 min arc with fixation through increasing amounts (0 to 4 D) of dioptric blur. However, without blur there was no statistically significant effect on fixation stability of varying the target contrast from 7 to 84%. The variability of vergence was not significantly influenced in either condition. Like other target parameters investigated in previous studies, dioptric blur and contrast have relatively inconsequential effects on fixation stability, indicating that the signals used to control fixation are largely independent of stimulus parameters. Practically, the findings suggest that when patients' eye movements are recorded for clinical reasons, the results are unlikely to be affected seriously by the presence of residual refractive error.

Contrast Sensitivity↗

Transpedicular fixation with Zielke instrumentation in the treatment of thoracolumbar and lumbar injuries.

STUDY DESIGN: Sixty-five patients who underwent transpedicular fixation for thoracolumbar and lumbar injuries were studied for type of injury, the severity of paralysis, the degree of postoperative correction, and instrumentation failures. OBJECTIVES: To evaluate the surgical approaches and the selection of instrumentation to determine indications for using the transpedicular fixation procedure. SUMMARY OF BACKGROUND DATA: Various transpedicular fixation devices have been used for different type of injuries, and satisfactory postoperative results were not obtained in some studies. METHODS: Forty patients had burst fractures, 19 had fracture dislocations, and six had chance-type fractures. An anterior decompression procedure was used for most cases of burst fracture and some cases of fracture dislocation where anterior compression factors were present. The Zielke or modified Zielke system was used as an internal fixator for posterior segmental fixation. RESULTS: No patient had neurologic deterioration after surgery. Twenty of 28 patients with incomplete lesions improved postoperatively according to Frankel grades. The instrumentation failed in only one patient, in whom a nonunion developed. CONCLUSION: With transpedicular fixation, it is possible to provide solid internal fixation that is circumscribed to the injured vertebral segments. The elasticity of the Zielke rod makes it an excellent transpedicular fixation device because it is easily attached and reduction is easily performed. Anterior decompression with fusion needs to be used with transpedicular fixation in the treatment of injuries (especially burst fractures).

Adult↗

Biomechanical properties of anterior thoracolumbar multisegmental fixation: an analysis of construct stiffness and screw-rod strain.

STUDY DESIGN: Three types of anterior thoracolumbar multisegmental fixation were biomechanically compared in construct stiffness and rod-screw strain. OBJECTIVES: To investigate the effects of rod diameter and rod number on construct stiffness and rod-screw strain in anterior thoracolumbar multisegmental instrumentation. SUMMARY OF BACKGROUND DATA: No studies have been undertaken to investigate the biomechanical effects of rod diameter and rod number in thoracolumbar anterior instrumentation. METHODS: Ten fresh-frozen calf spines (T13-L5) were used. After intact analysis, a total discectomy and transection of the ALL and PLL were performed at L1-L2, L2-L3, and L3-L4 with intervertebral reconstruction using carbon fiber cages. Three types of anterior fixation were then performed at L1-L4: 1) 4.75-mm diameter single-rod, 2) 4.75-mm dual-rod, and 3) 6.35-mm single-rod systems. Single screws at each vertebra were used for single-rod and two screws for dual-rod fixation. These systems share the same basic design except rod diameter. Nondestructive biomechanical testing was performed and included compression, torsion, flexion-extension, and lateral bending. Construct stiffness and rod-screw strain of the three reconstructions were compared. RESULTS: The 6.35-mm single-rod fixation significantly improved construct stiffness compared with the 4.75-mm single rod fixation only under torsion (P < 0.05). The 4. 75-mm dual rod construct resulted in significantly higher stiffness than did both single-rod fixations (P < 0.05), except under compression. No statistical differences were observed in rod-screw strain between the two types of single rods, whereas dual-rod reconstruction exhibited less rod-screw strain (P < 0.05). CONCLUSIONS: For single-rod fixation, increased rod diameter neither markedly improved construct stiffness nor affected rod-screw strain, indicating the limitations of a single-rod system. In thoracolumbar anterior multisegmental instrumentation, the dual-rod fixation provides higher construct stiffness and less rod-screw strain compared with single-rod fixation.

Animals↗

Interlaboratory comparison in HercepTest assessment of HER2 protein status in invasive breast carcinoma fixed with various formalin-based fixatives.

Although formalin-based fixatives are used in pathologic laboratories, there is no strictly standardized fixation protocol in Japan. To examine interlaboratory variation caused by different conditions of fixation in the assessment of human epidermal growth factor receptor (HER) 2 status on pathologic tissues, 274 archival invasive breast carcinomas from 5 different laboratories were evaluated using the HercepTest. In 1 laboratory in which 10% neutral buffered formalin was used, as recommended by the manufacturer, the overexpression rate was 22.4% and fell within the statistical expected range (20%-30%) for HER2 overexpression in breast carcinomas. The overexpression rates in the other 4 laboratories, in which either 20% nonbuffered formalin or 15% neutral buffered formalin was used, were near the expected range for HER2 overexpression. To clarify the influence of prolonged formalin fixation on the HercepTest, we compared 1-day with 7-day fixations using 36 cases fixed with 20% nonbuffered formalin. Of the 36 cases, 7 showed 3+ staining with 1-day fixation and sustained the same scoring results with 7-day fixation, although the staining intensities in these cases were reduced with the prolonged fixation. These results indicated that the immunohistochemical assessment of HER2 status with the HercepTest was comparatively resistant to prolonged fixation conditions and provided stable staining results in positive cases, particularly 3+ patients.

Antibodies, Monoclonal↗

Salvage of failed internal fixation of intertrochanteric hip fractures.

Most intertrochanteric hip fractures treated with internal fixation heal. If nonunion or early loss of fracture fixation occurs, treatment options include prosthetic replacement and revision internal fixation. The purpose of the current study was to evaluate the results of revision internal fixation and bone grafting for salvage of failed internal fixation of intertrochanteric hip fractures. Between 1981 and 2000, 20 patients with 20 intertrochanteric fractures who had initial internal fixation that failed were treated with revision open reduction and internal fixation and bone grafting. The mean age of the patients was 58 years (range, 21-86 years). The mean clinical followup was 27 months (range, 3-120 months), and mean radiographic followup was 22 months (range, 3-120 months). Eleven patients were treated with an angled blade plate (seven, 95 degrees; two, 90 degrees; one, 110 degrees; and one, Harris blade plate), five with a dynamic hip screw, three with a dynamic condylar screw, and one with a Zickel nail. Autograft bone was used in 17 patients and allograft bone was used in three patients. Nineteen of 20 nonunions healed (95%). Sixteen of the 19 patients who achieved healing reported no pain and three had mild pain (related to retained hardware); all were ambulatory. Two patients had perioperative complications (10%): one wound dehiscence, and one severe hyponatremia. In properly selected patients, revision internal fixation with bone grafting for failed open reduction and internal fixation of intertrochanteric hip fractures can provide a high rate of union and good clinical results with a low rate of complications.

Adult↗

Biomechanical superiority of plate fixation for proximal tibial osteotomy.

Proximal tibial osteotomies require secure and durable fixation to allow early range of motion; however, biomechanical data comparing commonly used fixation methods are lacking. The current study was done to quantify the dynamic biomechanical performance of blade staple fixation and plate fixation of simulated proximal tibial osteotomies. A 15 degrees proximal tibial osteotomy was done on each of 18 synthetic adult composite tibias. Blade staples were used as the means of fixation in nine tibias; plate fixation was used in the remaining nine tibias. The specimens were stressed cyclically in sinusoidal loading whose peak compression and tension loads imitate those measured during normal gait. Device performance was quantified by measuring displacement at the osteotomy site and the number of cycles to failure. Plate fixation had a greater fatigue life than staples (eight plates surviving past 200,000 cycles versus one blade staple) and showed a trend toward less displacement (0.69 mm versus 0.97 mm). Plate fixation of proximal tibial osteotomies offers better fixation and dynamic mechanical performance than blade staples.

Adult↗

Magnetic field coil measurements of the accuracy of extreme gaze ocular fixation.

PURPOSE: (1) To assess the adequacy of the magnetic field coil method (MFCM) for measures of extreme gaze ocular fixation; and (2) to measure the accuracy of ocular fixation for a short duration at gaze angles of 10 to 40 degrees. METHODS: Seven subjects participated. Two experiments were performed on the same day. In both experiments, the head and eye were rotated in opposite directions. In the first experiment, search coil slippage was assessed using photographs of a scleral search coil worn on one eye. These photographs were taken with a slitlamp biomicroscope equipped with a digital photography system as subjects rotated the eyes through nasal angles up to 40 degrees. In the second experiment, measures of ocular position were made using the MFCM as the eye was rotated through nasal angles up to 40 degrees to fixate a light-emitting diode target under two conditions. In one condition, subjects simply aimed the eye at the target. In the other, subjects placed a foveal afterimage on the target as the target was viewed through a pinhole. RESULTS: Temporal search coil slippage >10 arc min was noted for three subjects. For one subject, this slippage occurred at gaze angles > or =20 degrees, whereas for the other subjects, the slippage appeared at gaze angles of > or =30 degrees. Ocular fixation had to be assessed from the differences in fixation position between the two conditions in the second experiment because of search coil slippage and because the relationship between the anterior and posterior portions of the eyes changed for some individuals as the gaze angle changed. Fixation was usually accurate (within 10 arc min). There were occasional instances where fixation errors >15 arc min occurred, but we believe that all but one of these cases were attributable to poor localization of the afterimage. CONCLUSION.: Scleral search coils can slip on some individuals in extreme gaze. Search coils may also be inadequate to indicate the direction of the fovea in extreme gaze for some individuals because the relationship between the anterior and posterior portions can change with gaze angle. Once artifacts associated with the MFCM and localization of the afterimage are accounted for, ocular fixation is almost always within 10 arc min of the fixation target at gaze angles of 10 to 40 degrees.

Adult↗

A cost/utility analysis of open reduction and internal fixation versus cast immobilization for acute nondisplaced mid-waist scaphoid fractures.

BACKGROUND: Open reduction and internal fixation and cast immobilization are both acceptable treatment options for nondisplaced waist fractures of the scaphoid. The authors conducted a cost/utility analysis to weigh open reduction and internal fixation against cast immobilization in the treatment of acute nondisplaced mid-waist scaphoid fractures. METHODS: The authors used a decision-analytic model to calculate the outcomes and costs of open reduction and internal fixation and cast immobilization, assuming the societal perspective. Utilities were assessed from 50 randomly selected medical students using the time trade-off method. Outcome probabilities taken from the literature were factored into the calculation of quality-adjusted life-years associated with each treatment. The authors estimated medical costs using Medicare reimbursement rates, and costs of lost productivity were estimated by average wages obtained from the U.S. Bureau of Labor Statistics. RESULTS: Open reduction and internal fixation offers greater quality-adjusted life-years compared with casting, with an increase ranging from 0.21 quality-adjusted life-years for the 25- to 34-year age group to 0.04 quality-adjusted life-years for the > or =65-year age group. Open reduction and internal fixation is less costly than casting ($7940 versus $13,851 per patient) because of a longer period of lost productivity with casting. Open reduction and internal fixation is therefore the dominant strategy. When considering only direct costs, the incremental cost/utility ratio for open reduction and internal fixation ranges from $5438 per quality-adjusted life-year for the 25- to 34-year age group to $11,420 for the 55- to 64-year age group, and $29,850 for the > or =65-year age group. CONCLUSIONS: Compared with casting, open reduction and internal fixation is cost saving from the societal perspective ($5911 less per patient). When considering only direct costs, open reduction and internal fixation is cost-effective relative to other widely accepted interventions.

Adult↗

Inhibition of N2 fixation in soybean is associated with elevated ureides and amino acids.

Decreased N2 fixation in soybean (Glycine max) L. Merr. during water deficits has been associated with increases in ureides and free amino acids in plant tissues, indicating a potential feedback inhibition by these compounds in response to drought. We evaluated concentrations of ureides and amino acids in leaf and nodule tissue and the concurrent change in N2 fixation in response to exogenous ureides and soil-water treatments for the cultivars Jackson and KS4895. Exogenous ureides applied to the soil and water-deficit treatments inhibited N2 fixation by 85% to 90%. Mn fertilization increased the apparent catabolism of ureides in leaves and hastened the recovery of N2 fixation following exogenous ureide application for both cultivars. Ureides and total free amino acids in leaves and nodules increased during water deficits and coincided with a decline in N2 fixation for both cultivars. N2 fixation recovered to 74% to 90% of control levels 2 d after rewatering drought-stressed plants, but leaf ureides and total nodule amino acids remained elevated in KS4895. Asparagine accounted for 82% of the increase in nodule amino acids relative to well-watered plants at 2 d after rewatering. These results indicate that leaf ureides and nodule asparagine do not feedback inhibit N2 fixation. Compounds whose increase and decrease in concentration mirrored the decline and recovery of N2 fixation included nodule ureides, nodule aspartate, and several amino acids in leaves, indicating that these are potential candidate molecules for feedback inhibition of N2 fixation.

Acetylene↗

The Mallett Fixation Disparity Test: influence of test instructions and relationship with symptoms.

Fixation disparity is a minute ocular misalignment under conditions of binocular single vision and is typically detected in primary eye care practices in the UK using the Mallett Unit Fixation Disparity Test. This instrument creates natural viewing conditions, when the patient's binocular system is fused using both central and peripheral fusion locks. This allows the examiner to determine the minimum prism power that eliminates the fixation disparity: the associated phoria or aligning prism. The spherical power that eliminates the fixation disparity, the aligning sphere, can also be determined. The near Mallett Unit Fixation Disparity Test has been shown to have good sensitivity and specificity for detecting symptomatic heterophoria. Cases of decompensated heterophoria tend to have a fixation disparity and the aligning prism or aligning sphere is a good indicator of the correction that will render the heterophoria compensated. The purpose of this study was, for the first time, to investigate the effect of test instructions on the results of the Mallett Unit Fixation Disparity Test. In study 1, we surveyed and observed practitioners to determine the instructions that are typically used. In study 2, we compared results obtained with this "standard" method of questioning with a more "specific" form of questioning that has been suggested in the literature. The participants for study 2 were 105 patients aged 7-70 years who were randomly selected from those attending a community optometric practice. Significantly different results were obtained with the two sets of instructions. The specific form of questioning revealed more cases of fixation disparity and the results with this method showed a better correlation with symptoms. This only held for near vision: for distance vision, symptoms were not significantly correlated with the presence of fixation disparity. This agrees with previous work with the Mallett unit, which showed a significant relationship with symptoms only at near. We also found that patients with more severe symptoms had greater degrees of aligning prism. Our study supports previous work indicating that the Mallett unit is a useful tool for detecting symptomatic heterophoria at near. However, we found that the testing method is important: patients need to be asked not just whether the nonius strips are aligned but also whether one or both of the strips ever moves. More research is needed to investigate the significance of precise test instructions in other optometric and orthoptic tests.

Adolescent↗

In vitro comparison between a DCP and external fixator for pancarpal arthrodesis in the dog.

Many clinical reports have previously documented the advantages of plates or external fixations but surgeons prefer to use plate for better acceptance by the owner, less dressing and a decreased rate of infection. In order to address complications of carpal surgery, infections, arthritis and osteomyelitis, external fixation is warranted. Twelve canine forelimbs, free of abnormality, were tested under compression to evaluate the in vitro biomechanical behaviour of the normal carpus. This behaviour was compared with that of a stabilized carpus stabilised by panarthrodesis with either plates (8 hole 3.5 mm dynamic compression plate (DCP), or a type II external fixator. Deformation vs. load was recorded after compression with a testing machine in conjunction with a digital data acquisition system. Yield load, maximal load to failure and stiffness were then calculated. Variables were significantly greater for arthrodesis with external fixation and plates than for intact forelimbs. The stiffness and the MLF after stabilisation with plates or external fixators did not differ significantly. At MLF, fracture of the third metacarpus appeared with plates, whereas deformations of the pins and bars of the external fixators increased with load. Stabilisation with plate or external fixation allowed more load to failure than the intact carpus. The biomechanical behaviour of both methods of arthrodesis was identical during loading. However, after rupture, external fixation was more plastic. External fixation, which is less often used than plates for pancarpal arthrodesis, mainly due to the occurrence of sepsis, may be useful for arthrodesis of the carpus in big or active dogs.

Animals↗

A role for natural antibody in the pathogenesis of leprosy: antibody in nonimmune serum mediates C3 fixation to the Mycobacterium leprae surface and hence phagocytosis by human mononuclear phagocytes.

We have previously determined that complement receptors on human mononuclear phagocytes and complement component C3 in nonimmune serum mediate phagocytosis of the intracellular bacterial pathogen Mycobacterium leprae, the agent of leprosy. We have also determined that C3 fixes selectively to the major surface glycolipid of M. leprae, phenolic glycolipid 1 (PGL-1). In this study, we have explored the role of natural antibody in nonimmune serum in C3 fixation and C1q binding to M. leprae and PGL-1. At serum concentrations within the range at which phagocytosis of M. leprae is maximal, C3 fixation was mediated by both the classical and the alternative complement pathways. At the low end of this serum concentration range (2.5%), C3 fixation was mediated predominantly by the classical pathway. Consistent with a role for both pathways, C3 fixation to M. leprae was enhanced by the addition of either pure C1q to C1q-depleted serum or pure factor B to factor B-depleted serum. C3 fixation to M. leprae was strictly antibody dependent regardless of the serum concentration used. C3 fixation to M. leprae occurred in nonimmune serum but not in agammaglobulinemic serum unless heat-inactivated nonimmune serum or small amounts of pure immunoglobulin G (IgG) or IgM were added. C3 fixation by both the alternative and the classical complement pathways was mediated by antibody, and the antigen-binding portion of the antibody molecule was required. C3, IgG, IgM, and C1q were readily detected on the surface of M. leprae. Consistent with the previously demonstrated exclusive role of the classical complement pathway in C3 fixation to PGL-1, C1q bound to PGL-1 in a dose-dependent fashion; C1q binding was evident in > 1.25% nonimmune serum. C1q binding to PGL-1 was strictly antibody dependent. When PGL-1 was incubated with pure C1q, little or no C1q bound to PGL-1 unless heat-inactivated nonimmune serum or pure IgG or IgM was added. When PGL-1 was incubated in nonimmune serum, C3 bound directly to PGL-1 and not to anti-PGL-1 antibody, since the amount of C3 bound to PGL-1 was not reduced by acid elution of the antibody. However, the amount of C3 bound to PGL-1 was markedly reduced by hydroxylamine treatment, providing evidence for C3 fixation via a covalent ester bond. Nonimmune serum contained antibody to all four major M. leprae surface carbohydrates. Relative to PGL-1, nonimmune serum contained more antibody to the other surface carbohydrates.(ABSTRACT TRUNCATED AT 400 WORDS)

Antibodies, Bacterial↗

Fixation sequences made during visual examination of briefly presented 2D images.

Eye movements made by eighteen observers in response to brief (3 s) presentations of eleven different images, each in three forms (unfiltered, high-pass filtered and low-pass filtered), have been analysed in order to identify both repeated sequences of fixations and image locations which attract re-fixations. It is shown that eye-movement traces made by different observers in response to the same image have few common temporal sequences involving the same fixation locations, even for sequences of only two fixations. There is a greater incidence of such sequences in eye-movement traces made by the same observer in response to two presentations of the same image, but average numbers are still low. Conserved sequences involving more than two identical locations occur at a much lower frequency, and the incidence of repeated sequences is not increased if consideration is restricted to regions of the image which attract large numbers of fixations. It is concluded that the temporal sequence in which fixations are made is not a significant factor in the analysis of the eye-movement data considered in this report. Calculations based on a least squares index of similarity are consistent with this conclusion. The analysis shows a relatively high incidence of re-fixation on certain locations in the images and there is evidence that such re-fixations are a significant factor in the high similarity between fixation locations established by different observers when viewing the same image.

Eye Movements↗

Determination of the localization of gangliosides using anti-ganglioside antibodies: comparison of fixation methods.

Many studies have examined the localization of gangliosides using anti-ganglioside antibodies, although widely differing conclusions have been reached. We now demonstrate that the apparent localization of gangliosides can be greatly influenced by the fixation method. Using monoclonal antibody (MAb) A2B5 (which reacts with a variety of gangliosides), hippocampal neurons were labeled at the cell surface when incubated with the antibody before fixation, but when incubated after fixation the cells displayed a variety of labeling patterns, depending on the fixation method. Biochemical analysis demonstrated that some of the fixatives (particularly acetone and methanol) significantly reduced or completely depleted cellular gangliosides, implying that the immunoreactivity observed with A2B5, and with other antibodies, was not due to gangliosides. When neurons were incubated with an anti-GD1b antibody prefixation, uniform labeling of the plasma membrane was observed, but after ganglioside depletion using biochemical inhibitors of ganglioside synthesis no cell surface labeling was detected. However, even in cells depleted of gangliosides, labeling of both the cell surface and intracellular compartments was observed when the anti-GD1b antibody was applied after fixation. Moreover, after fixation, antibodies to GM4 and GD2 reacted with hippocampal neurons, although these gangliosides are absent from these neurons. In contrast, the JONES antibody (which reacts with 9-O-acetylated GD3) labeled neurons with a similar pattern, essentially irrespective of the fixation method. These observations demonstrate that great care must be taken in assigning gangliosides to specific cell populations or to intracellular locations solely on the basis of use of anti-ganglioside antibodies, and suggest that optimal fixation conditions must be established for each anti-ganglioside antibody.

Animals↗

Compaction of a bone dowel in the tibial tunnel improves the fixation stiffness of a soft tissue anterior cruciate ligament graft: an in vitro study in calf tibia.

BACKGROUND: Despite increasing attention on fixation of a soft tissue anterior cruciate ligament graft in the tibia, there have been no studies on the use of a bone dowel as a joint line fixation device for promoting fixation properties, especially stiffness at the time of implantation. PURPOSE: To determine whether compacting a bone dowel into the tibial tunnel improves fixation stiffness, yield load, and resistance to slippage of a soft tissue anterior cruciate ligament graft. STUDY DESIGN: Controlled laboratory study. METHODS: A double-looped tendon graft was fixed at the distal end of the tibial tunnel with a WasherLoc in 24 calf tibias. The tibial tunnels were treated with or without a dowel of cancellous bone. The bone dowel was harvested from the tibial tunnel and then compacted into a tapered space anterior to the anterior cruciate ligament graft as a joint line fixation device. A cyclic load and measurement test was administered to determine fixation stiffness, yield load, slippage, and failure mode. RESULTS: The specimens with the bone dowel had 58 N/mm more stiffness (P = .04); however, the yield load and resistance to slippage were similar in specimens with and without the bone dowel. CONCLUSIONS: A bone dowel harvested from the tibial tunnel can be used as a joint line fixation device in series with a distal fixation method to improve initial fixation stiffness and increase the fit, which is known to enhance tendon graft-to-bone healing in the tibia.

Animals↗