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At least 865 records · Page 48Linked to original sources
PubMed1984

Topical carcinogenesis by N-methyl-N-nitrosourea in Syrian hamster cheek-pouch and oral mucosa.

Pouches of weanlings were painted 3 times a week for 4 weeks with 0.5 per cent NMU in acetone, using a technique designed to localize oncogenesis. Ventral lingual mucosa was painted at the same time. Premalignant, dysplastic lesions or carcinomas arose in pouches of 88 per cent of animals that survived from 8 to 54 weeks, but the majority of these lesions were not localized to the painted area. Biliary hyperplasia, papillomas of the forestomach and abnormalities of the incisor teeth were found in a small number of animals. These changes suggested that systemic absorption of NMU had occurred. No lesions were induced beneath the tongue but occasional premalignant and malignant changes were found in other parts of the mouth.

Absorption↗
PubMed1984

Touch domes and Merkel cells in hamster cheek pouch epithelium.

Minute dome-shaped elevations on the epithelial surface of hamster buccal pouch were counted in full-thickness whole mounts of detached pouch epithelium. Domes were examined by light and electron microscopy. Each dome consisted of thickened epithelium containing numerous Merkel cells with associated unmyelinated terminal axons. The pouch domes have structural characteristics in common with Haarscheiben, or touch domes, described in hair-bearing skin in man and other mammals and must not be confused with focal hyperplastic or precancerous lesions.

Animals↗
PubMed1989

Elemental and cytochemical localization of calcium in rat cheek epithelium.

Energy-dispersive X-ray microanalysis, combined with scanning electron microscopy, was applied to 18 microns thick freeze-dried cryosections to determine the level of calcium in successive layers of the epithelium. This indicated low levels of calcium in basal cells, high in spinous cells, moderate in granular cells, lowest in the inner keratin and highest in the outer keratin layers. The potassium pyroantimonate technique was used to study the cytochemical distribution of Ca2+. The reaction product, calcium pyroantimonate (Ca-PA), was generated from cellular and intercellular calcium by perfusion of the anaesthetized rat with a solution of potassium pyroantimonate in glutaraldehyde. Ca-PA was localized in nuclei, mitochondria, lysosomal-type bodies and membrane-coating granules. A denser Ca-PA distribution was found between upper spinous and lower granular cells. The proposed identification of Ca2+ as a major component in Ca-PA was confirmed by EDTA decalcification and X-ray microprobe analysis of the reaction product. Thus X-ray microanalysis in combination with cytochemistry can localize Ca2+ in a growing and differentiating tissue such as stratified squamous epithelium.

Animals↗
PubMed1991

A cheek island flap for the lower eyelid.

An island transposition flap for use in lower eyelid reconstruction and the treatment of ectropion is described. The flap is based on a subcutaneous pedicle 1 to 2 cm lateral to the outer canthus. Its long axis extends inferolaterally from this point, and the donor site can be closed directly. The skin of the full width of the lid can be replaced, and where skin replacement is not required the flap may be de-epithelialised and used to provide bulk and support only. The flap has also been successfully lined with a nasal muco-chondral graft. Clinical examples are described, including the treatment of cicatricial, senile and paralytic ectropion, and the repair of defects ranging from skin alone to the full thickness of the lid.

Adult↗