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PubMed1984

A possible role for potassium and pyruvate in the modulation of sperm motility in the rabbit oviducal isthmus.

Spermatozoa were recovered from the isthmus of the rabbit oviduct at 4 and 11 h post coitum using several defined flushing media. The motility of spermatozoa in the isthmic flushings was subsequently analysed from video recordings. There was little sperm movement in the native isthmic fluid, but vigorous flagellar activity and hyperactivated movement were induced by flushing the isthmus with 0.25 M-sucrose, apparently an effect of dilution. Flushing with a complex culture medium resulted in similar stimulation of sperm movement. When pyruvate was present in the medium, hyperactivated flagellar bending was stimulated, whereas these movements were virtually absent when glucose alone was present. The stimulating effect of dilution was less pronounced when the flushing medium contained 50 mM-potassium. When the isthmus was flushed with media containing 50 mM-K+ and the K+ concentration was lowered to 5 mM during a washing procedure, large-amplitude flagellar movements were sequentially suppressed and restored. The restoration of large-amplitude movements was enhanced when pyruvate was present in the 5 mM-K+ washing medium. These results suggest that alterations in the concentration of both K+ and pyruvate may have a role in regulating the motility of rabbit spermatozoa in the oviducal isthmus, K+ being inhibitory and pyruvate stimulatory.

Animals↗
PubMed1986

Quantification of sperm motility by a turbidimetric assay. Correlation to cellular respiration.

The turbidimetric method according to SOKOLOSKI et al. (Fertil. Steril. 28, 1337-1341, 1977) was used for quantitative measurement of the motility of washed bovine spermatozoa. The method is based on the registration of the increase in absorbance (A538) which is produced by spermatozoa entering the light path after run from the bottom of a photometer cuvette. This increase in absorbance is dependent on the amount of motile cells and their average velocity. The increase in absorbance related to the total cell concentration was found to be a highly reproducible quantity (motility index M). Inhibitors of dynein ATPase (vanadate) and of the mitochondrial ATP-production (KCN, carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP), oligomycin) produced a diminution of the motility index M in a concentration-dependent manner. Under conditions of oxidative energy metabolism (lactate as substrate) a linear relation between respiration and M existed. Such a relationship was found under treatment of spermatozoa with KCN or vanadate as well as for untreated sperm suspensions. The linear correlation to the respiratory rate justifies to use the proposed motility index as a relative measure of the average motility of a sperm sample.

Adenosine Triphosphatases↗