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Differential backfilling of interneuron populations based upon axon projections in a lobster abdominal ganglion.

Intersegmental interneurons in the lobster second abdominal ganglion were differentially stained by simultaneously backfilling their axons in the anterior and posterior hemiconnectives with nickel and cobalt ions. When precipitated with rubeanic acid the nickel and cobalt formed different colors in the cell bodies depending upon the location of the axon(s) in the connectives. In the most completely stained preparations 55 ascending, 56 descending, and 25 bidirectional interneurons were observed. Soma diameters ranged from 15-120 micron. One of the somata was located in the connective. The differential staining technique has advantages over conventional backfilling techniques for examining the morphological relationships of populations of neurons having different axon projections.

Animals↗

Some claustral neurons projecting to various neocortical areas show morphological differences.

The morphology of claustral neurons projecting to the motor, somatosensory, auditory and visual cortical areas in the rat was analyzed by means of combination of axonal retrograde transport and morphometric analysis. Fluoro-Gold (FG) injections placed into various cortical fields resulted in labeling in the claustrum four neuronal types: pyramidal with thick main dendrite, oval with a few thin dendrites spreading out in various directions, fusiform possessing two main dendrites arising from opposite poles of the cell body and polygonal. Pyramidal neurons prevailed in populations of neurons projecting to the motor cortex of the contralateral hemisphere. Oval neurons outnumbered other types in populations projecting to the somatosensory, auditory and visual cortical fields. The number of fusiform and polygonal neurons did not exceed at 12.5% together in any populations. Neurons projecting to the contralateral hemisphere were the largest claustral neurons (mean cross-section are 167.19 +/- 2.9 micron 2) whereas neurons projecting to the motor cortex where the largest claustral neurons projecting ipsilaterally (141.89 +/- 2.22 micron 2). There was no significant difference between neurons projecting to the somatosensory (113.46 +/- 1.9 micron 2) cortex and to the visual (111.8 +/- 1.4 micron 2) cortex whereas neurons related to the auditory are (95.98 +/- 1.75 micron 2) were the smallest claustral neurons. These observations pointed out that the morphology of claustral neurons is closely related to a cortical area to which they send axons.

Animals↗

Smoking habits and attitude to smoking in the study population of the MONICA Project Augsburg.

Data concerning smoking habits were derived from the first MONICA-Augsburg survey 1984/85 based on a random two stage cluster sample of the 25-64 year old population of the city of Augsburg and the two surrounding rural districts (n = 5312). Data were gathered by a standardized interview. Response was 79%. Overall, about 36% of men and 18% of women are regular cigarette smokers (i.e. smoking more than one cigarette per day). In both sexes smoking decreases with increasing age. In men 43% of the 25-34 year old participants are regular smokers and 29% in the age group 55-64. In women 29% of the youngest age group but only 9% aged 55-64 are current regular smokers. In male regular cigarette smokers the mean amount of cigarettes consumed per day is 21, in women 14 cigarettes. The mean amount of nicotine and tar consumed per day is 17 mg and 277 mg in men, 10 mg and 159 mg in women. On the average men start smoking at the age of 18 and women at the age of 21. In both sexes the percentage of regular smokers, who are willing to change their smoking habits, is very high. In each age and sex group about 60% of all regular smokers affirmed their willingness to quit smoking in the future. It is hoped, that this willingness of the population to change their smoking behavior will be supported by suitable education and intervention programs.

Adult↗

Cardiovascular risk groups and mortality in an urban swedish male population: the Malmö Preventive Project.

OBJECTIVES: To describe the size, overlap and mortality of four cardiovascular risk groups, in order to give a scientific background for the prevention of cardiovascular disease in a representative urban population. SETTING: Section of Preventive Medicine, Department of Medicine, Malmö University Hospital, Malmö, Sweden. SUBJECTS: Between 1974 and 1984 22444 men born between 1949 and 1921, constituting 75% of the total male population in these age groups, took part in a comprehensive screening examination aimed at detecting risk factors for cardiovascular disease. INTERVENTIONS: Those at high-risk of developing cardiovascular disease were referred to their general practitioner or to special clinics for hypertension, hyperlipidaemia and diabetes. The follow-up, which lasted until the end of 1991, averaged 12.2 years. MAIN OUTCOME MEASURES: Total death (n = 1450) and death from ischaemic heart disease (IHD) (n = 471). RESULTS: Hypertension was found in 13%, hypercholesterolaemia in 19% and diabetes mellitus in 2.6% of the subjects; 49% of the subjects smoked. Multiple risk factors were found in over 17% of the total cohort. Despite the intervention, all-cause mortality during follow-up was increased three-fold in smokers and in men with hypercholesterolaemia, four-fold in hypertensive men and five-fold in men with diabetes, compared to men with no risk factors. The vast majority of deaths (81%) occurred in men who smoked, had hypertension or had high serum cholesterol. Ischaemic heart disease (IHD) was increased five-fold in smokers, seven-fold in men with hypercholesterolaemia, nine-fold in hypertensive men and 12-fold in men with diabetes. Again, the vast majority of IHD deaths (86%) occurred in the first three categories. Combinations of risk factors substantially increased total mortality as well as IHD mortality. CONCLUSIONS: The large proportion (64%) of the population with risk factors for cardiovascular disease and the substantially (5-12-fold) increased IHD mortality in those risk groups, calls for actions aimed at preventing premature IHD deaths. Such action should include measures directed towards the whole population and comprehensive treatment programmes for high-risk individuals, including intervention to stop smoking. The substantial overlap between risk factors calls for one high-risk clinic caring for all risk groups.

Adult↗

Prevalence of dementia and its subtypes in the Japanese American population of King County, Washington state. The Kame Project.

Studies of Asian populations generally have reported prevalence rates for dementia similar to those of predominantly Caucasian populations, but relative prevalence rates of Alzheimer's disease and vascular dementia have differed. Between May 1, 1992 and May 1, 1994, the prevalence rates of dementia, Alzheimer's disease, and vascular dementia were examined in the Japanese American population aged over 65 years in King County, Washington State. A total of 3,045 eligible individuals were identified in a census of persons who were of at least 50% Japanese heritage. Of 1,985 persons who participated in the baseline examination, 382 individuals of 450 sampled from all cognitive performance strata received a diagnostic evaluation. A total of 107 cases with a Clinical Dementia Rating (CDR) of > or = 1 met criteria for dementia according to the Diagnostic and Statistical Manual, 3rd edition, revised (DSM-III-R); 58 of these cases were diagnosed with Alzheimer's disease and 24 with multi-infarct dementia. The estimated prevalence rate for all dementias was 6.3% (95% confidence interval 5.9-6.8). Prevalence rates for dementia increased continuously with age and were 30%, 50%, and 74% for participants aged 85-89, 90-94, and > or = 95 years, respectively; for Alzheimer's disease, prevalence rates were 14%, 36%, and 58% for these three age groups. Rates for Alzheimer's disease were generally higher among women; for multi-infarct dementia, rates for men and women were similar. In the institutional population, the prevalence rate was 66%, and in the community, 2.9%. Persons with lower education had higher overall rates of dementia than those with higher education, but this tendency became weak and inconsistent when rates were age-stratified. The prevalence of dementia in this geographically defined population of Japanese Americans was somewhat higher than prevalence rates reported from Japan, and the distribution of dementia subtypes more closely resembled that found in Caucasian populations in North America and Europe than previously reported in Asian populations.

Age Distribution↗

Greater incidence of hospitalized myocardial infarction among Mexican Americans than non-Hispanic whites. The Corpus Christi Heart Project, 1988-1992.

BACKGROUND: Since Mexican Americans have adverse patterns of risk factors for myocardial infarction relative to non-Hispanic whites, the incidence of myocardial infarction should be greater among Mexican Americans than among non-Hispanic whites. This expectation conflicts with reports generated from death certificate registries. METHODS AND RESULTS: Data regarding myocardial infarction attacks and incident events were collected for a 4-year period in the Corpus Christi Heart Project, a population-based surveillance project for hospitalized coronary heart disease events. For both women and men, Mexican Americans experienced greater hospitalization rates for both attacks and incident events than non-Hispanic whites. Age-adjusted attack rate ratios comparing Mexican Americans with non-Hispanic whites were 1.59 (95% CI, 1.05 to 2.41) and 1.31 (95% CI, 1.18 to 1.45) among women and men, respectively. Corresponding incidence ratios were 1.52 (95% CI, 1.28 to 1.80) and 1.25 (95% CI, 1.10 to 1.42). CONCLUSIONS: This is the first report documenting greater incidence of hospitalized myocardial infarction among Mexican Americans than among non-Hispanic whites, a biologically plausible finding given the risk factor patterns observed in the Mexican-American population. Public health planners and clinicians should be aware of the importance of myocardial infarction as a health problem in the Mexican-American population. Culturally appropriate prevention strategies should be developed for and tested in Mexican-American populations.

Adult↗

Common-pool resources and population genomics in Iceland, Estonia, and Tonga.

This paper addresses the application of the ethical concept of trust and the legal and political concept of public trust to population genomics projects in Iceland, Estonia, and Tonga. Focusing on trust and public trust, the paper explores analogies between the genomics projects and the treatment of other common-pool resources, making use of the notion of trust as an ethical demand, derived from the works of Emmanuel Levinas and Knud Eljer Løgstrup. The paper discusses the degree to which the ethical demands for trust and public trust have been established and maintained in the three national population genomics projects.

Confidentiality↗

Some problems of future demographic processes in China.

"In this paper, which is based on a projection model of rural-urban population dynamics established by the authors, we make use of the investigative data of the one percent national population sample of 1987 and analog calculations on almost 200 demographic parameters to clarify the problematic relations between some key issues in China's future demographic processes. We advance a number of suggestions for the reference of policy-makers and planners."

Asia↗

What do people want from dentistry? An action research approach.

Issues surrounding access to dentistry will be presented from information gathered during an action research project targeting three population groups. This project was conducted by students in the Dental Hygiene Degree Completion Program at the University of British Columbia and by the dental hygiene diploma students at Camosun College. In contrast to typical empirical dental studies which are validated by statistical presentation, action research uses an interview process and qualitative analysis to identify what people really think, believe and do. College students, independent-living seniors, and low-income families were interviewed to determine how they accessed dental care, how they liked their dental visits, and how they preferred to receive dental health information. Cost, convenience and fear of pain were significant factors which influenced whether people attended the dental office. Caring dental health providers, comfort and pleasant physical surroundings were important features of a dental office visit. Dental health practices, such as brushing and flossing, were predominantly learned from the dental profession at the dental office, through school programs, or from parents at home. This data challenged our previous assumptions about these groups. As the goal of this type of research is "action", the information gathered will lead to action plans at the community and professional level.

Adult↗

An epidemiological study of migraine with aura in the San Severo general population: a pilot research project of cooperation between neurologists and general practitioners.

OBJECTIVES: (A) To define the lifetime prevalence of migraine with aura (MA) in patients recruited in general practices for an epidemiologic study by the University of Parma Headache Centre from the general population of San Severo, Italy, and (B) to assess the recognition of MA in general practice. METHODS: The study was conducted over a period of 5 consecutive months (January to May 2001) on patients aged 18-65, taken from the following general practice populations: (a) from the patient population of 4 general practitioners (GPs; 3,616 patients) and (b) from the patient population of 12 GPs (12,996 patients). The clinical diagnosis of MA was subsequently confirmed by a headache specialist. RESULTS: For objective A, 648 patients (420 women and 228 men) were interviewed; MA diagnosis was confirmed in 10 patients (7 women and 3 men), lifetime MA prevalence being 1.5%. For objective B, 150 'suspected' MA cases (96 women and 54 men) were reported by the 12 GPs; however, the diagnosis of MA was confirmed in only 3 cases (2 women and 1 man). CONCLUSION: The prevalence of MA observed in our small sample of the general practice patient population was comparable with that observed in the general population of San Severo, Italy. The results also suggest that GPs may be overestimating the number of cases of MA in their patient population.

Adolescent↗

Lessons learned from the Healthy Heart Community Prevention Project in reaching the African American population.

The Healthy Heart Community Prevention Project (HHCPP) is an ongoing program of cardiovascular risk identification and modification in the African American community in New Orleans, Louisiana. The program targets low socioeconomic status African American populations, which continue to have higher rates of coronary heart disease, stroke, and overall cardiovascular mortality than the general population. Among the HHCPP's initiatives have been the use of barbershops and beauty salons as blood pressure screening sites. Church-based programs, in which ministers provide "healthy heart sermons," as well as screenings at sports events, where volunteers provide health advice, have proven to be successful aspects of the program. The HHCPP has also offered educational sessions to a broad cross-section of health professionals, including physicians as well as nurse practitioners and nutritionists. Future initiatives include the establishment of cholesterol screening programs.

Black or African American↗

The demography of Kiribati: estimates from the 1985 census.

"This paper presents an analysis of the 1985 census [of Kiribati] and compares projections made on the basis of that analysis with the 1990 enumeration. Comparison is made throughout with the estimates derived from [the] 1978 census." Information is provided on population growth and structure, child and adult mortality, marriage and first birth, and fertility.

Birth Order↗

Laminar and areal differences in the origin of the subcortical projection neurons of the rat somatosensory cortex.

Fluorescent retrograde tracing techniques were employed in a double-labelling paradigm to determine the distribution of corticospinal, corticotectal, and corticotrigeminal projection neurons in layer Vb of the adult and neonatal rat somatosensory cortex. The double-labelling paradigm allowed a direct comparison of the cortical distribution of neurons projecting to each target and identification of neurons projecting to more than one target. In the adult rat, each population of projection neurons was found to have a unique laminar and/or areal distribution. Corticospinal projection neurons were located throughout the width of layer Vb in the medial granular portion of somatosensory cortex, while corticotrigeminal projection neurons were distributed throughout the width of layer Vb in the more laterally located dysgranular portion of somatosensory cortex. Corticotectal projection neurons were located more superficially in layer Vb than either corticospinal or corticotrigeminal projection neurons and found scattered throughout both dysgranular and granular somatosensory cortex. Each combination of subcortical injections also resulted in double labelling a small percentage of uniquely distributed neurons. These distribution differences coupled with measurements of cell size allowed us to identify the parent population of the dual projection neurons. Subpopulations of corticotectal neurons also project to the brainstem trigeminal complex and to the spinal cord. Subpopulations of corticotrigeminal neurons also project to the spinal cord, and a proportion of corticotrigeminal neurons projects to at least two targets within the brainstem trigeminal complex (nucleus principalis and subnucleus interpolaris). In the adult rat, corticospinal neurons (as defined by either laminar position or somal size) did not appear to give off collaterals to either the superior colliculus or brainstem trigeminal complex. In the neonatal rat, double-labelled neurons which project to both the spinal cord and the tectum are distributed throughout the full width of layer Vb, rather than restricted to the superficial portion of the layer as in the adult rat. Further, it appears as if the ontogenetic change in the laminar distribution of corticospinal and tectal projection neurons is achieved by mechanisms of selective process elimination rather than cell death. These results are discussed in terms of both the developmental factor which may contribute to the discrete distribution of cortical projection neurons found in the adult and the functional significance of bifurcating projection neurons.

Animals↗