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Non-uniformity of surface temperatures after microwave heating of poultry meat.

Microwave energy has the potential to raise the surface temperatures of meat rapidly for a short period of time sufficient to reduce bacterial numbers significantly without causing physical changes to the meat. Studies have investigated the ability of a standard domestic microwave oven (2450 MHz; IEC 1191 W), an experimental repeatable microwave cavity (2450 MHz; IEC 1139 W) and a number of shielding techniques to achieve uniform surface temperature distributions on pieces of poultry meat. In the domestic oven temperature differences of up to 60 and 80 degrees C were found between different points on the surface of the same sample after 30 s and 3 minutes of heating respectively. The use of a standard cavity and shielding resulted in a difference of less than 5 degrees C between the average surface temperature on the edge and middle of regular slabs of chicken after 30 s exposure. Results show that microwave heating, using 2450 MHz, is unlikely to produce consistently uniform enough surface temperatures on meat to reduce bacterial numbers without surface damage.

Animals↗

Temperature and weight loss profiles of model cakes baked in the microwave oven.

Model cake systems were formulated with wheat and rice starches at hydration levels of 112.5% and 137.5% (flour weight basis) and baked in a microwave oven at power levels of 80% and 100%. Temperature profiles and weight loss profiles of the cakes baked in the microwave oven were compared with those of the cakes baked conventionally. One cake was baked at a time, and three replications of each treatment were used. Center and edge temperatures of microwave cakes increased significantly with increasing moisture content of the batter and oven power. Weight loss of the cakes was dependent on oven power, starch type and hydration levels. Cakes baked in the microwave oven had greater weight loss than the cakes baked in convection mode. Wheat starch cakes had greater volumes than rice starch cakes. Rice starch cakes were more tender than wheat starch cakes.

Cooking↗

Microwave techniques in agriculture.

Microwave techniques, methods and instrumentation can be utilized in agriculture to improve the efficiency of crop production, handling and processing, and improve the quality of products. Pertinent microwave theory relative to such applications is discussed, and a review of microwave research on agricultural problems is presented. A wide range of applications is treated briefly, including examples of successful applications of microwaves in agriculture, and some future prospects are discussed.

Agriculture↗

Effects of 2.45 GHz microwave radiation and heat on mouse spermatogenic epithelium.

The rear halves of the bodies of anaesthetized male C3H mice were exposed for 30 min to 2.45 GHz microwave radiation and the effects on the testes were compared to those produced by direct heating. Effects were observed which are consistent with the hypothesis that heat damage is the primary effect of microwave exposure. Damage measured six days after exposure ranged in severity from depletion of the spermatocytes to extensive necrosis of the germinal epithelium. Temperature-sensitive probes implanted in the testes revealed a threshold effect for depletion of the spermatocytes of approximately 39 degrees C and an LD50 6 (50 per cent cell death after 6 days) of about 41 degrees C after microwave exposure or direct heating. The corresponding effective threshold effect and LD50 6 expressed in terms of absorbed microwave power were 20 W kg-1 and 30 W kg-1. However, it is probable that a conscious animal is better able to regulate testicular temperature and hence adjust to higher dose-rates.

Animals↗

Microwave plasma conversion of volatile organic compounds.

A microwave-induced, steam/Ar/O2, plasma "torch" was operated at atmospheric pressure to determine the feasibility of destroying volatile organic compounds (VOCs) of concern. The plasma process can be coupled with adsorbent technology by providing steam as the fluid carrier for desorbing the VOCs from an adsorbent. Hence, N2 can be excluded by using a relatively inexpensive carrier gas, and thermal formation of oxides of nitrogen (NOx) is avoided in the plasma. The objectives of the study were to evaluate the technical feasibility of destroying VOCs from gas streams by using a commercially available microwave plasma torch and to examine whether significant byproducts were produced. Trichloroethene (TCE) and toluene (TOL) were added as representative VOCs of interest to a flow that contained Ar as a carrier gas in addition to O2 and steam. The O2 was necessary to ensure that undesirable byproducts were not formed in the process. Microwave power applied at 500-600 W was found to be sufficient to achieve the destruction of the test compounds, down to the detection limits of the gas chromatograph that was used in the analysis. Samples of the postmicrowave gases were collected on sorbent tubes for the analysis of dioxins and other byproducts. No hazardous byproducts were detected when sufficient O2 was added to the flow. The destruction efficiency at a fixed microwave power improved with the addition of steam to the flow that passed through the torch.

Adsorption↗

A comparison of microwave heating and proteolytic pretreatment antigen retrieval techniques in formalin fixed, paraffin embedded tissues.

Antigen retrieval (AR) is a technique that re-exposes epitopes in formalin fixed, paraffin embedded sections and makes them detectable by immunohistochemistry. We compared the effects of two AR procedures, enzyme digestion and microwave heating, on immunostaining of vimentin and desmin in formalin fixed, paraffin embedded tissues. Our results showed that AR is necessary for vimentin and desmin immunostaining in tissues fixed in formalin for more than 48 h. With prolonged fixation times, microwave heating showed better results than enzyme digestion for AR. The same results were obtained using 1% zinc sulfate or Citra Plus solution as retrieval solutions for microwave heating. We recommend microwave heating for AR, because it is easier to use and produces better results compared to enzyme treatment.

Animals↗

Using microwave irradiation in Marchi's method for demonstrating degenerated myelin.

Conventional methods for histological preparation of degenerated myelin are time-consuming and difficult. The purpose of our study was to shorten the time required for the procedure and to obtain better quality results for light microscopic demonstration of degenerated myelin in the central and peripheral nervous systems by using microwave irradiation. Rat brain and sciatic nerve were used for the study. The middle cerebral artery was occluded and the sciatic nerve was cut to produce myelin degeneration. Marchi's method was used for staining degenerated myelin. Fixation for light microscopy that would take two days using the conventional procedure was completed in 16.5-18.5 min using microwave irradiation. While staining of degenerated myelin requires 10 days for the conventional Marchi method, we decreased it to 7 h for brain tissue and 1 h for sciatic nerve by using the microwave oven. Moreover, a better quality preparation was achieved in the groups stained under microwave irradiation than those prepared by the conventional method.

Animals↗

Effects of exposure of the ear to GSM microwaves: in vivo and in vitro experimental studies.

The effects of mobile phone (GSM) microwaves on the ears of guinea pigs were investigated in two in vivo experiments and one in vitro experiment. In the first experiment, three groups of eight guinea pigs had their left ear exposed for 1 h/day, 5 days/week, for 2 months, to GSM microwaves (900 MHz. GSM modulated) at specific absorption rates (SARs) of 1, 2 and 4 W/kg respectively, and a fourth group was sham-exposed. Distortion-product otoacoustic emissions (DPOAEs) were measured for each ear before exposure, at the end of the 2-month exposure period, and 2 months later. In the second experiment, the same protocol was applied to eight sham-exposed and 16 exposed guinea pigs at 4W/kg, but the auditory brainstem response (ABR) thresholds were monitored. Repeated-measures ANOVA showed no difference in DPOAE amplitudes or in ABR thresholds between the exposed and non-exposed ears and between the sham-exposed and exposed groups In the course of the second experiment, acute effects were also investigated by measuring once, in all animals, ABR thresholds just before and just after the 1-h exposure: no statistically significant difference was observed. In vitro, the two organs of Corti (OCs) of newborn rats (n=15) were isolated and placed in culture. For each animal, one OC was exposed for 24-48 h to 1 W/kg GSM microwaves, and the other was sham-exposed. After 2-3 days of culture, all OCs were observed under light microscopy. They all appeared normal to naive observers at this stage of development. These results provided no evidence that microwave radiation, at the levels produced by mobile phones, caused damage to the inner ear or the auditory pathways in our experimental animals.

Analysis of Variance↗

Coupled field analysis of heat flow in the near field of a microwave applicator for tumor ablation.

Microwave tumor ablation (MTA) offers a new approach for the treatment of hepatic neoplastic disease. Reliable and accurate information regarding the heat distribution inside biological tissue subjected to microwave thermal ablation is important for the efficient design of microwave applicators and for optimizing experiments, which aim to assess the effects of therapeutic treatments. Currently there are a variety of computational methods based on different vascular structures in tissue, which aim to model heat distribution during ablation. This paper presents results obtained from two such computational models for temperature distributions produced by a clinical 2.45 GHz MTA applicator immersed in unperfused ex vivo bovine liver, and compares them with measured results from a corresponding ex vivo experiment. The computational methods used to model the temperature distribution in tissue caused by the insertion of a 5.6 mm diameter "wandlike" microwave applicator are the Green's function method and the finite element method (FEM), both of which provide solutions of the heat diffusion partial differential equation. The results obtained from the coupled field simulations are shown to be in good agreement with a simplified analysis based on the bio-heat equation and with ex vivo measurements of the heat distribution produced by the clinical MTA applicator.

Animals↗

A system for studying effects of microwaves on cells in culture.

An improved design of the fluid-filled waveguide-exposure chamber is described for studying effects of microwave radiation on cells in vitro. The system with a micropipette sample holder may be used as a prototype to isolate the apparent nonthermal factor of microwave radiation on cells in culture from those effects resulting from cell temperature rise. This system also allows more precise calibration of incident and absorbed microwave energies. Compared with control, somatic cells of the Chinese hamster exhibited a lower rate of growth and difference in morphology after 2450 MHz microwave radiation for 20 min at a power density of 500 mW/cm2.

Animals↗

Physiological and behavioral effects of prolonged exposure to 915 MHz microwaves.

Long-Evans male adult rats were exposed for 16 weeks to 915-MHz CW microwaves at an average power density of 5 mW/cm2. The resulting dose rate was 2.46 (+/- 0.29 SEM) mW/g. The animals were exposed eight hours a day, five days a week, for a total of 640 h in a monopole-above ground radiation chamber while housed in Plexiglas cages. Daily measures of body mass and of food and water intake indicated no statistically significant effects of microwave irradiation. Measures by activity wheels and stabilimetric platforms of spontaneous locomotion indicate than mean activity levels increased about 25% after microwave exposure, but the findings are doubtful statistical significance (Ps < .10 but > .05). Studies of blood sampled after 2, 6, 10, and 14 weeks of exposure revealed alterations of free sulfhydryls. Measures of levels of urinary 17-ketosteroids at weeks 1, 5, 9, and 12 of exposure, and measures of brain hypothalamic tissue, and of mass of adrenals, heart, and liver at the end of the 16-week period, revealed no significant differences between irradiated and control animals. Cortical EEGs sampled after conclusion of microwave exposures also revealed no significant differences.

17-Ketosteroids↗

Reliability of microwave heating for hemoderivative thawing.

The spoiling of coagulation factors, proteic patterns and specific activity has been comparatively determined in fresh frozen plasma, cryoprecipitates and hemodiagnostic sera, thawed in a 37 degrees C water bath and in a microwave oven. Effects of conventional and microwave heating are not significantly different, while results of the latter technique are rapid and aseptic. Previously, heating performances of a commercial microwave oven have been investigated for deionized water, saline solutions, and bovine serum. Furthermore, plastic containers of hemoderivatives have been tested to assure that no toxic products are released during microwave heating.

ABO Blood-Group System↗

Rapid determination of total Kjeldahl nitrogen using microwave digestion.

A closed-vessel microwave digestion process for the determination of total Kjeldahl nitrogen (TKN) has been developed for sewage and wastewater. TKN values obtained from the microwave digestion method were in excellent agreement with those of the thermal digestion method. The accuracy of both analytical methods is comparable. In comparison to the conventional thermal digestion, the microwave method shortened the time required for complete digestion from 4 h to 25 min, and also decreased the maximum digestion temperature from 380 degrees C to 200 degrees C. This developed method may contribute to a significant reduction in sample digestion time, resulting in an increase in analytical throughput. The microwave digestion method developed in this study could be a rapid and efficient means for TKN determination for sewage wastewater and sludge.

Hot Temperature↗

Nonthermal microwave radiations affect the hypersensitive response of tobacco to tobacco mosaic virus.

OBJECTIVES: The aim of the present study was to evaluate the effects of nonthermal extremely high-frequency microwave radiations in a plant-based bioassay, represented by tobacco plants reacting to tobacco mosaic virus with a hypersensitive response leading to the appearance of necrotic lesions at the infection sites. DESIGN: This study was performed blind and different experimental protocols on tobacco plants inoculated with tobacco mosaic virus were used. BIO-OBJECTS: Tobacco plants (Nicotiana tabacum L. cultivar Samsun) carrying the resistance gene N against tobacco mosaic virus. INTERVENTIONS: Tobacco plants or leaf disks were either directly or indirectly (water-mediated) irradiated using a medical device, designed for microwave resonance therapy. It produces nonthermal weak-intensity extremely high-frequency radiations, either modulated at extremely low frequency or in continuous flux of waves, coupled with a nonthermal red/near-infrared radiation. OUTCOME MEASUREMENTS: The working variable was the number of hypersensitive lesions per leaf disk. RESULTS: Both direct and indirect nonthermal microwave radiations led to significant effects on the hypersensitive response of tobacco plants: modulated radiations generally induced a resistance increase, whereas a continuous flux of waves induced a resistance decrease with direct treatments only. CONCLUSIONS: Nonthermal microwave radiations are effective on the hypersensitive response of tobacco to tobacco mosaic virus and their low-frequency modulation seems to be more bioactive than the continuous-flux of waves, particularly in the indirect water-mediated treatments.

Biological Assay↗

Microwave technology for the rapid thawing of frozen blood components.

Based on a continuing need to provide a more rapid response to requests for thawed fresh frozen plasma, the authors evaluated plasma thawing with the use of a microwave oven and compared it with conventional 37 degrees C waterbath thawing methods. Their results indicate that microwave-thawed plasma contains precipitated denatured protein (mainly albumin and fibrinogen) and that there is a significant reduction of coagulation Factors IX, X, XI, and fibrinogen compared with fresh plasma. They also measured levels of di-ethyl hexyl phthalate after microwave thawing and found its rate of accumulation similar to that of the 37 degrees C waterbath. More importantly, fundamental principles of microwave heating preclude uniform temperatures being maintained throughout the thawing of plasma; hence, the denaturation of plasma proteins is expected to occur under even low heating conditions.

Blood Preservation↗

Immunohistochemical study of p53 expression in microwave-fixed, paraffin-embedded sections of colorectal carcinoma and adenoma.

Expression of p53, a tumor-suppressor gene product, was studied immunohistochemically in microwave-fixed, paraffin-embedded sections of 84 colorectal carcinomas and 44 adenomas. Using a monoclonal antibody (PAb1801), nuclear p53 was detected successfully in 51 of 84 (60.7%) cases of carcinomas, and no stain for p53 was demonstrated in the adjacent normal mucosa. The results in the microwave-fixed, paraffin-embedded sections correlated with those in the frozen sections. The incidence of p53 expression in colorectal carcinomas was high in the cases with distant metastasis, but it was not affected by clinicopathologic features such as tumor size or depth of invasion. In colorectal adenomas, only 4 of 44 (9%) adenomas expressed p53. This expression of p53, however, was restricted to only a few glands within tubular adenomas with mild dysplasia. Thus, p53 protein was expressed preferably by malignant tumors of the colorectum. The present study demonstrated the usefulness of microwave fixation to preserve p53. The immunohistochemical detection of p53 in microwave-fixed, paraffin-embedded sections of colorectal carcinoma and adenoma can provide valuable information about the mechanism of carcinogenesis in colorectal epithelium.

Adenoma↗

Assessment of testicular core temperatures using microwave thermography.

A new method for the reliable assessment of testicular core temperature using microwave technology is presented. This study was designed to assess whether alterations in testicular thermoregulation could be reliably demonstrated in patients with clinically apparent varicoceles (n = 36), in those with idiopathic male infertility (n = 52) and in fertile donors (n = 20) using this new microwave thermographic technique. The measurements obtained were found to be reliable and reproducible. Testicular core temperature measurements were significantly different between the groups (P < 0.001). Furthermore, there was a temperature gradient between the scrotal neck and the testicular core in all groups; testicular core temperatures were lower than scrotal neck temperatures. The magnitude of this temperature difference was also significantly different (P < 0.001) between the groups. Microwave testicular thermography is a new technique that is safe and accurate. Preliminary results suggest altered testicular thermoregulation in a group of patients with impaired spermatogenesis with and without varicocele. Testicular temperature profiles obtained by microwave thermography may be of value in the assessment of infertile men with or without a varicocele.

Adult↗

Microwave fixation: in situ tick (Acari: Ixodidae) histoanatomy, thin sectioning of tick tissues, and antigen preservation in mouse spleen.

Microwave irradiation was used for the fixation of eggs, nymphs, and adult Boophilus spp. ticks. Although optimal temperatures for fixation of the different tick stages varied, heating to 58 degrees C of adult ticks submerged in either PBS or fixative was found to be sufficient. After microwave fixation, whole adult ticks, hand held, were sectioned with a sharp razor blade. The resulting sections revealed the in situ histoanatomy of the tick. Thin sections of ticks were obtained after either paraffin or polyester wax embedding. Microwave fixation combined with polyester wax embedding made serial thin sections of the different stages of Boophilus ticks possible. The technique preserved antigens as demonstrated by the immunostaining of lymphocytes and erythrocytes infected with Babesia microti in mouse tissues subjected to the same treatment as the ticks. With the microwave fixation-polyester wax technique, the specimen preparation time from fixation to the section on the glass slide was reduced to less than 8 h.

Animals↗