Survival of microfilariae released in vitro by the filarial worm, Dipetalonema viteae.
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Refractoriness to Brugia pahangi microfilarial infection could be induced in the normally susceptible Aedes togoi mosquitoes by intrathoracic injection with crude thoracic homogenate of the refractory Aedes albopictus mosquitoes. The crude thoracic homogenate contained three Sephadex G-200 protein profiles of which the first profile showed strongest inhibition to the parasite development.
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A radiolabel uptake viability assay for Onchocerca cervicalis using [3H]2-deoxy-D-glucose in Hanks' balanced salt solution, pH 7.5, at 30 C is described and compared to the traditional visual motility assay. A correlation of r = 0.92 between the assays was found, with the radiolabel uptake method apparently a more sensitive indicator of microfilarial viability.
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An evaluation of the microfilarial index has been carried out in a randomised population, during April and May 1986, to determine the efficacy of long course mass chemotherapy. A single dose of DEC at 6 mg/kg was distributed to the whole population of the island, every 4 months, in 1985 and 1986. The survey was done three months after the treatment. 1,307 thick blood films have been collected in night-time. The prevalence of microfilaremia was 1.9% as compared with 17.1% in 1981 in the conditions. The control measures were considered as quite satisfactory, and it was decided to continue mass chemotherapy together with vector control.
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Seven patients infected with the filarial worm Loa loa received a treatment by cytapheresis in an attempt to lower the microfilaraemia. Microfilarial levels of between 6,000 and 38,500 ml, before extraction, were reduced, according to the case, by between 47 and 97% (mean 76%). The diethylcarbamazine chemotherapy which followed in 6 of 7 patients showed no sign of any of the serious side-effects which often occur in these type of cases. Due to its practicality and the fact that it is well tolerated, both clinically and biologically, cytapheresis would seem to represent the best method for initially treating loaiasis with high microfilaremia.
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