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PubMed1985

Activity changes of three nucleolytic enzymes during the life cycle of Saccharomyces cerevisiae.

Conditions were established for the assay of three nucleolytic enzymes: a Mg2+-independent endoribonuclease, a Mg2+-dependent endonuclease, and a Mg2+-dependent 5'-exonuclease in Saccharomyces cerevisiae cell extracts. The changes in the activities of these enzymes were determined throughout the life cycle of the organism. As the cells progressed from the exponential to the stationary growth phase, the specific activities of the Mg2+-independent endoribonuclease and of the Mg2+-dependent 5'-exonuclease increased, whereas the Mg2+-dependent endonuclease decreased. During sporulation the Mg2+-independent endoribonuclease and the Mg2+-dependent 5'-exonuclease increased several-fold over the first 10 h, but, since a similar increase was seen in nonsporulating control cells, the increases did not appear to be related to sporulation. However, the specific activity of the Mg2+-dependent endonuclease showed a sporulation-related increase during the first 3 h of sporulation, with a subsequent decline to very low levels. The specific activity of this enzyme increased again during germination to the levels seen in exponential phase cells. The Mg2+-independent endoribonuclease and the Mg2+-dependent 5'-exonuclease showed little change during germination of the ascospores. The high specific activity of the Mg2+-independent endoribonuclease during periods of nutrient deprivation is in agreement with the proposed role for this enzyme in the degradation of rRNA under these conditions.

Chromatography, Agarose↗
PubMed2004

The binding of histone deacetylases and the integrity of zinc finger-like motifs of the E7 protein are essential for the life cycle of human papillomavirus type 31.

The E7 oncoprotein of high-risk human papillomaviruses (HPVs) binds to and alters the action of cell cycle regulatory proteins such as members of the retinoblastoma (Rb) family of proteins as well as the histone deacetylases (HDACs). To examine the significance of the binding of E7 to HDACs in the viral life cycle, a mutational analysis of the E7 open reading frame was performed in the context of the complete HPV type 31 (HPV-31) genome. Human foreskin keratinocytes were transfected with wild-type HPV-31 genomes or HPV-31 genomes containing mutations in HDAC binding sequences as well as in the C-terminal zinc finger-like domain, and stable cell lines were isolated. All mutant genomes, except those with E7 mutations in the HDAC binding site, were found to be stably maintained extrachromosomally at an early passage following transfection. Upon further passage in culture, genomes containing mutations to the Rb binding domain as well as the zinc finger-like region quickly lost the ability to maintain episomal genomes. Genomes containing mutations abolishing E7 binding to HDACs or to Rb or mutations to the zinc finger-like motifs failed to extend the life span of transfected keratinocytes and caused cells to arrest at the same time as the untransfected keratinocytes. When induced to differentiate by suspension in methylcellulose, cells maintaining genomes with mutations in the Rb binding domain or the zinc finger-like motifs were impaired in their abilities to activate late viral functions. This study demonstrates that the interaction of E7 with HDACs and the integrity of the zinc finger-like motifs are essential for extending the life span of keratinocytes and for stable maintenance of viral genomes.

Binding Sites↗
PubMed2003

Life cycle analysis and sewer solids.

The search for sustainable ways of living has necessitated a new look at the way in which water services are provided. The new paradigm includes whole-system perspectives for each of the primary criteria groups: social, environmental and economic. Whilst Life Cycle Analysis (LCA) techniques have been used successfully for products, they are much less used to assess processes. Nonetheless there is much to learn from the use of LCA for a much wider range of applications. An application is described whereby LCA has been used to determine energy, mass flows and environmental impacts for a number of sewer-related options for handling sewer solids, using the SimaPro software. This work has been part of a wider study to provide enhanced decision support systems for water utilities.

Conservation of Natural Resources↗
PubMed2006

PbGCbeta is essential for Plasmodium ookinete motility to invade midgut cell and for successful completion of parasite life cycle in mosquitoes.

When malaria parasites enter to mosquitoes, they fertilize and differentiate to zygotes and ookinetes. The motile ookinetes cross the midgut cells and arrive to the basement membranes where they differentiate into oocysts. The midgut epithelium is thus a barrier for ookinetes to complete their life cycle in the mosquitoes. The ookinetes develop gliding motility to invade midgut cells successfully, but the molecular mechanisms behind are poorly understood. Here, we identified a single molecule with guanylate cyclase domain and N-terminal P-type ATPase like domain in the rodent malaria parasite Plasmodium berghei and named it PbGCbeta. We demonstrated that transgenic parasites in which the PbGCbeta gene was disrupted formed normal ookinetes but failed to produce oocyst. Confocal microscopic analysis showed that the disruptant ookinetes remained on the surface of the microvilli. The disruptant ookinetes showed severe defect in motility, resulting in failure of parasite invasion of the midgut epithelium. When the disruptant ookinetes were cultured in vitro, they transformed into oocysts and sporozoites. These results demonstrate that PbGCbeta is essential for ookinete motility when passing through the midgut cells, but not for further development of the parasites.

Adenosine Triphosphatases↗
PubMed1996

Light and electron microscopic identification of Cyclospora species in the small intestine. Evidence of the presence of asexual life cycle in human host.

This is the first case of cyclosporiasis in which the parasite was clearly demonstrated in a duodenal biopsy by light microscopy. Electron microscopy identified the stages of sporozoite, trophozoite, schizont, and merozoite. Although only asexual forms were identified in our case, the sexual cycle must have taken place in the human host, because oocysts were detected in stools of the patients. Therefore, it appears that Cyclospora species require only a single host to complete its entire life cycle. Despite the heavy infection, only enterocytes were invaded. The lamina propria and submucosa were not involved. The morphology of Cyclospora in the intestine is similar to that of Isospora, but differs from that of Cryptosporidium. The morphology of the oocyst of Cyclospora resembles that of Cryptosporidium, but differs from that of Isospora. Thus, a combined study of both stool and intestinal biopsy should readily distinguish Cyclospora from Cryptosporidium and Isospora.

AIDS-Related Opportunistic Infections↗
PubMed1976

Cytological and chemical changes in cell walls of Rhodotorula gracilis. III. Characteristics and life cycle of the yeast.

The phenotype and genotype of six strains of the genus Rhodotorula Harrison and of one strain of the genus Cryptococcus Phaff et Fell, with anomalous thickening of cell walls were investigated. The present studies showed that the strains investigated represent different stages of the life cycles of the genus Rhodosporidium Banno. The anomalous thickening of the cell walls can be explained by extreme conditions resulting in the formation of surviving forms (teliospores, chlamydospores).

Bacterial Proteins↗
PubMed1977

[Cytochemical study of different stages in the life cycle of Toxoplasma gondii. VII. Oxidation--reduction enzymes in the cyst forms].

Dehydrogenases of glycolysis, Kreb's cycle, and pentose-phosphate shunt were detected in cystozoites of Toxoplasma gondii strain SS-119 with various degrees of activity. A mixed oxidative metabolism may be postulated on this stage of the toxoplasma life cycle. Besides, the activity of cytochrome oxidase was detected in cystozoites; the addition of cytochrome c to the incubation medium significantly intensified the reaction intensity. Of interest seems the observation of a layer of higher enzymatic activity in the host brain tissue in the immediate neighbourhood with the cyst body. This may be regarded as the host cells' (or tissue') response to the presence of the parasite's alien body.

Dihydrolipoamide Dehydrogenase↗
PubMed1984

[Calculation of Steel's cell loss factor and of the cellular kinetic parameters for a population with an exponential growth of the cell number and an equal probability of cell death in all phases of the life cycle].

A method of calculation of parameter m (equal to a doubled probability of birth of cells which did not pass into phase G0 from the mitotic cycle) and of the proliferative pool was shown to be the same for the exponential growth phase of cell population with the equal probability of cell death (lambda = const) at all phases of the life cycle (the Steel model of type E), and for the case "lambda = 0" (the Steel model of type B). The method is proposed for the calculation of parameter lambda and of the Steel cell lose factor phi = lambda/[lambda + [ln 2)/tD)]. It is noted that the value of parameter phi for the type E model can be estimated by the Steel formula: phi congruent to 1-tDpot/tD--where tD is the doubling time of the cell number, and tDpot = tcln 2/ln m. Here tc is the mean mitotic cycle duration.

Cell Count↗
PubMed1999

The life cycle of Ascogregarina taiwanensis (Apicomplexa:Lecudinidae).

A number of features, including large size of the trophozoite, host-specificity, developmental synchrony and evident migration behavior, of Ascogregarina taiwanensis make it suitable as a model for studies of parasite-host relationships. In this article, Wei June Chen describes the life cycle of As. taiwanensis; and raises some areas for future work, including the interaction between the parasite and its host, inhibition of sexual reproduction by non-natural hosts and their mutual effects during the development.

Aedes↗
PubMed2003

Early events in the life cycle of JC virus as potential therapeutic targets for the treatment of progressive multifocal leukoencephalopathy.

The human polyomavirus, JC virus (JCV), is the etiological agent of progressive multifocal leukoencephalopathy (PML). PML occurs almost exclusively in the setting of severe and prolonged immunosuppression and it remains an important and life-threatening complication in the acquired immunodeficiency syndrome (AIDS) population. Several drugs that target DNA replication have shown efficacy at inhibiting JCV replication in vitro but none to date have shown in vivo efficacy. The authors' laboratory has been studying early events that contribute to infection of susceptible cells by JCV. They previously demonstrated that infection of glial cells by JCV requires clathrin-dependent endocytosis and that this early step in the viral life cycle can be blocked by the antipsychotic drug, chlorpromazine. As chlorpromazine is associated with the development of extrapyramidal symptoms that may be heightened in AIDS patients, the authors sought to test the atypical antipsychotic, clozapine, for antiviral activity against JCV. In this report, the authors show that clozapine is as effective as chlorpromazine at inhibiting infection. They further demonstrate that low-dose combinations of both drugs synergistically inhibit infection.

Antipsychotic Agents↗
PubMed1994

Life cycle evaluation of five biological types of beef cattle in a cow-calf range production system: II. Biological and economic performance.

Results from a 10-yr study of five dam breed groups in a range beef production system were used as inputs to a simulation model to evaluate life cycle biological and economic performance. Dam breed groups were straightbred Herefords (HH), Angus-Hereford and Simmental-Hereford F1 crosses (AH and 1S1H), 75% Hereford-25% Simmental (1S3H), and 25% Hereford-75%. Simmental (3S1H). Biological measures were number of lifetime matings, calves weaned and kilograms of calf weight sold per cow exposed, and metabolizable energy (ME) consumed per kilogram of calf weight sold and per kilogram of total weight sold. Simulated economic measures were break-even steer prices, total production costs, net profit per cow exposed, and net profit for a ranch of fixed size. The HH and 3S1H dams consumed the least ME per kilogram of total weight sold yet had the highest steer break-even prices and lowest profits. The F1 dams yielded consistently higher profits than other dam breed groups. Breed group rankings were not sensitive to prices paid for hay or calf prices relative to cow prices. Regression techniques were used to estimate maternal heterosis and maternal breed substitution effects from the simulated data. Maternal heterosis effects were large and highly significant for all measures of system performance and were desirable for all traits except ME/kilogram of total weight sold. Maternal heterosis for net profit was nearly $70.cow-1.yr-1 and $20,400/yr for a ranch of fixed size (2,700 AUM range forage). Maternal breed substitution effects were generally much smaller than heterosis effects.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed↗
PubMed1994

A laboratory study on the life cycle and feeding behaviour of Arrenurus madaraszi (Acari: Arrenuridae) parasitizing Anopheles mosquitoes.

Although mosquitoes of several genera were collected from one locality in Thiruvananthapuram, Kerala State, India, only Anopheles spp. carried larval Arrenurus madaraszi; 21.5% of the An. hyrcanus and 19.0% of the An. vagus were infested. However, the whole life cycle of Arr. madaraszi could be replicated in the laboratory using Aedes albopictus, Ae. vittatus or Armigeres subalbatus as foster hosts. The host specificity observed in the field is therefore ecological, not physiological. In the laboratory, the nymphs and adult mites were free-living and when offered ostracods, copepods and larvae of Ae. albopictus, Ae. vittatus, An. hyrcanus, An. vagus and Arm. subalbatus as food, they showed a preference for first instar mosquito larvae, particularly those of Ae. albopictus.

Aedes↗
PubMed2002

Nutritional impact of intestinal helminthiasis during the human life cycle.

Poor people in developing countries endure the burden of disease caused by four common species of soil-transmitted nematode that inhabit the gastrointestinal tract. Disease accompanying these infections is manifested mainly as nutritional disturbance, with the differing infections having their deleterious effects at different phases during the human life cycle. Reduced food intake, impaired digestion, malabsorption, and poor growth rate are frequently observed in children suffering from ascariasis and trichuriasis. Poor iron status and iron deficiency anemia are the hallmarks of hookworm disease. The course and outcome of pregnancy, growth, and development during childhood and the extent of worker productivity are diminished during hookworm disease. Less is known about the impact of these infections in children under 2 years of age. The severity of disease caused by soil-transmitted nematodes has consistently been found to depend on the number of worms present per person. Cost-effective measures based on highly efficacious anthelminthic drugs are now available to reduce and control disease caused by these infections.

Adolescent↗
PubMed1999

A life-cycle approach to ministry with the aging.

Notes that the demographic predictions of dramatic increase in the number of elderly members of local congregations will change the focus and emphasis in pastoral ministry in the next millennium as the needs of the aging take precedence. Argues for a comprehensive, life-cycle approach to ministries with the aging that integrates theology and the behavioral sciences. Offers examples and practical methods for ministering more effectively to this important and growing population in the local church.

Aged↗
PubMed2006

Brd4 is involved in multiple processes of the bovine papillomavirus type 1 life cycle.

Brd4 protein has been proposed to act as a cellular receptor for the bovine papillomavirus type 1 (BPV1) E2 protein in the E2-mediated chromosome attachment and mitotic segregation of viral genomes. Here, we provide data that show the involvement of Brd4 in multiple early functions of the BPV1 life cycle, suggest a Brd4-dependent mechanism for E2-dependent transcription activation, and indicate the role of Brd4 in papillomavirus and polyomavirus replication as well as cell-specific utilization of Brd4-linked features in BPV1 DNA replication. Our data also show the potential therapeutic value of the disruption of the E2-Brd4 interaction for the development of antiviral drugs.

Animals↗
PubMed2003

Fish full life-cycle testing for the weak estrogen 4-tert-pentylphenol on medaka (Oryzias latipes).

We studied the chronic effects of 4-tert-pentylphenol (4-PP) on reproductive status of medaka (Oryzias latipes) over two generations under continuous exposure, with the goal of verifying the applicability of the fish full life-cycle test (FFLC) for this weak estrogen with this species. The exposure of parental (F0) medaka to 4-PP was begun on embryos within 12 h after fertilization and continued for up to 101 d, with monitoring of embryological development, hatching, posthatch survival, growth, sexual differentiation, reproduction, and hepatic vitellogenin (VTG) levels under flow-through exposure to 4-PP at mean measured concentrations of 51.1, 100, 224, 402, and 931 microg/L. Eggs (F1) spawned from the F0 fish at 99, 100, and 101 d after hatch also were examined for hatchability, survival after hatching, growth, sexual differentiation, and hepatic VTG levels, until 61 d after hatch. In the FFLC with the F0 medaka, the lowest-observed-effect concentration (LOEC) of 4-PP for lethal and sublethal toxicity (as shown by growth inhibition) was 931 microg/L. The LOECs for estrogenic effects (as shown by abnormal sexual differentiation and VTG induction) were 224 and < or = 51.1 microg/L, respectively, and the LOEC for reproductive impairment was 224 microg/L. Therefore, the effective concentrations of 4-PP for abnormal sexual differentiation and reproductive impairment were about four times lower than those for lethal and sublethal toxicity. In the F1 medaka, the LOECs for sublethal toxicity and estrogenic effects were 224 and < or = 51.1 microg/L, respectively. This finding suggests that the continuous exposure to 4-PP over two generations induced these adverse effects at lower concentrations in the F1 generation than those in the F0 generation. Thus, 4-PP has estrogenic effects that reduce the reproductive potential of medaka. The results indicate that the FFLC with medaka is applicable to the evaluation of weak estrogens.

Animals↗
PubMedDate not supplied

[The life cycle and fine morphology of the embryophores in Microsomacanthus paraparvula (Cestoda: Hymenolepididae), a parasite of diving ducks in Chukotka].

It was found out, that the cestode Microsomacanthus paraparvula Regel, 1994 being a common parasite of diving ducks in Chukotka uses a caddisfly Grensia praeteria (Trichoptera) as an intermediate host in its life cycle. Mature fragments of the cestode have been collected from droppings of the experimentally infected nestling of the kittiwake Rissa tridactyla (non-specific host) and used for the fine morphology study of embryonic shells and for an infection of intermediate hosts.

Animals↗