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Unusual induction of slow-fast atrioventricular nodal reentrant tachycardia. Report of two cases.

INTRODUCTION: Generally, the induction of typical atrioventricular nodal reentrant tachycardia (AVNRT) occurs with a premature atrial stimulus that blocks in the fast pathway and proceeds down the slow pathway slowly enough to allow the refractory fast pathway time to recover. We describe two cases in which a typical AVNRT was induced in an unusual fashion. RESULTS: The first case is a 41-year-old man with paroxysmal supraventricular tachycardia. During the electrophysiology study, the atrial extrastimulus inducing the typical AVNRT was conducted simultaneously over the fast (AH) and the slow pathway (AH'). A successful ablation of the slow pathway was performed. During the follow-up no recurrence was noted. The second case is a 52-year-old woman with a Wolff-Parkinson-White syndrome due to a left posterior accessory pathway. After 5 minutes of atrioventricular reentrant tachycardia (AVRT) induced by a ventricular extrastimulus, a variability of the antegrade conduction was noted in presence of the same VA conduction. In fact, a short AH interval (fast pathway) alternated with a more prolonged AH intervals (slow pathway) that progressively lengthened until a typical AVNRT was induced. The ablation of the accessory pathway eliminated both tachycardias. DISCUSSION: A rare manifestation of dual atrioventricular nodal pathways is a double ventricular response to an atrial impulse that may cause a tachycardia with an atrioventricular conduction of 1:2. In our first case, an atrial extrastimulus was simultaneously conducted over the fast and the slow pathway inducing an AVNRT. This nodal reentry implies two different mechanisms: 1) a retrograde block on the slow pathway impeding the activation of the slow pathway from the impulse coming down the fast pathway, and 2) a critical slowing of conduction in the slow pathway to allow the recovery of excitability of the fast pathway. Interestingly, in the second case, during an AVRT the atrial impulse suddenly proceeded alternately over the fast and the slow pathway. The progressive slowing of conduction over the slow pathway until a certain point which allows the recovery of excitability of the fast pathway determines the AVNRT. This is a case of "tachycardia-induced tachycardia" as confirmed by the fact that the ablation of the accessory pathway eliminated both tachycardias.

Adult↗

Conceptual, spatial, and cue learning in the Morris water maze in fast or slow kindling rats: attention deficit comorbidity.

Rat lines selectively bred for differences in amygdala excitability, manifested by "fast" or "slow" kindling epileptogenesis, display several comorbid features related to anxiety and learning. To assess the nature of the learning deficits in fast kindling rats, performance was evaluated in several variants of a Morris water-maze test. Regardless of whether the location of the platform was fixed or varied over days (matching-to-place task), the fast rats displayed inferior performance, suggesting both working and reference memory impairments. Furthermore, when the position of the platform was altered after the response was acquired, fast rats were more persistent in emitting the previously acquired response. The poor performance of fast rats was also evident in both cued and uncued tasks, indicating that their disturbed learning was not simply a reflection of a spatial deficit. Moreover, fast rats could be easily distracted by irrelevant cues, suggesting that these animals suffered from an attentional disturbance. Interestingly, when rats received several training trials with the platform elevated, permitting them to develop the concept of facile escape, the performance of fast rats improved greatly. The performance disturbance in fast rats may reflect difficulties in forming a conceptual framework under conditions involving some degree of ambiguity, as well as greater distractibility by irrelevant cues. These various attributes of the fast rats may serve as a potentially useful animal model of disorders characterized by an attention deficit.

Amygdala↗

Microbiological investigation and nutritional evaluation of selected fast food meat.

The study was designed into two parts: the first part was to determine individual attitudes and beliefs toward fast food in general. One hundred individuals (15-45 yrs old) were involved in this study (50 males and 50 females). The second part of the study was carried out to evaluate microbiological contamination and nutritive value of the selected fast food meat (Hardee's fried burger, Saudi-American burger, kentucky fried chicken, Al-Baik broast chicken and shawerma beef). The results indicated that individuals 25-45 yrs. old were the most fast food consumers. The main reason behind increasing individual's preferences toward fast food was found to be for fun and inspiration. Among individuals under study 46% of males and 20% of females purchased fast food more than 4 times per week. Prevalence of overweight and obesity respectively were 38% and 22% among males and 34% and 14% among females. Bacillus cereus and E. coli were detected in a number of less than 10/g in all the selected fast food meat. The number of coliforms detected in Hardee's burger and Saudi-American burger were 10/g, while less than 10/g were detected in the remaining fast food meat. However, the number of Staph. aureus detected in Hardee's burger and Saudi-American burger was 20/g and 10/g respectively. On a per 100 g basis, energy (Kcal), protein (g), fat (g) and sodium (mg) content were found in the range of 179.62-295.29, 13.05-26.06, 8.9-21.13 and 640-920 respectively. Sodium content of all the selected fast food meat exceeded the recommended daily adequate intake for adults (males and females). The observations of the present study indicated the need for a nutrition education program to correct consumers' attitudes and beliefs towards fast food and to provide information on how a given menu item contributes to their dietary goal.

Adolescent↗

Quantitative comparison of the structural features of slow and fast neuromuscular junctions in Manduca.

The multiterminal slow and fast neuromuscular junctions of the moth Manduca sexta were compared using scanning, thin-section, and freeze-fracture techniques to see what structural features might underlie their functional differences. Slow neuromuscular junctions, here formed on tonic muscle fibers, produce a facilitating e.j.p. the amplitude of which is 1/5 to 1/3 the size of a fast excitatory junction potential (EJP) and the duration of which is nearly four times longer. A slow junction consists of a single terminal branch that is shorter in length than either of the pair of branches that a fast junction forms close together on the muscle fiber. Within the junction, slow nerve terminals exhibit longer, more frequent constrictions and are very varicose compared with fast. Since fast larval junctions on tonic muscle fibers are also varicose (Schaner and Rheuben, 1985), this is unlikely to represent an intrinsic property of the nerve. However, calculations of the length constants of the varicose versus nonvaricose shapes indicate that the effect of passive cable properties on normal functioning may act to limit the length of the slow terminals more than that of fast. Even though the varicose shape can be predicted to prolong the time course of the EJP, calculations show that, at the measured length, this would not explain the very long EJP that is observed. Within the neuromuscular junctions, the synapses are characterized on the muscle membrane by a patch of densely packed particles on the external leaflet and on the nerve membrane by a single linear active zone. The total number of synapses per slow junction is about 1/3 that of fast junctions. There is a weak correlation between average area of the individual postsynaptic particle patches and cross-sectional area of the muscle fibers that transcends nerve and muscle fiber types. The average lengths of active zones from the two types do not differ significantly. However, the number of particles per active zone in slow junctions is about 55% of the number in fast active zones. Chemically fixed slow nerve terminals have a greater density of synaptic vesicles remaining than do fast. If a proportion of the active zone particles represent structures directly involved in the probability of transmitter release, such as Ca++ channels, then the latter two characteristics may jointly reflect differences in capability to release and mobilize transmitter that would partly explain the different EJP amplitude and facilitation properties.

Lepidoptera↗

[Motor cortex control of fast and slow motoneurons innervating forelimb muscles of the cat].

Intracellular potentials of forelimb motoneurons of pyramidal cats were recorded by glass microelectrodes following repetitive stimulation of the contralateral pericruciate cortex, and patterns of the postsynaptic potentials (PSPs) evoked thereby were analysed. The motoneurons tested were identified by antidromic invasion from either of the following nerves: long (lo. T), lateral (la. T) and medial (med. T) heads of triceps, anconeus (Anc), biceps (Bi), deep radial (dR), median (M) and ulnar (U). The motoneurons were classified into fast and slow motoneurons on the basis of the duration of afterhyperpolarization (AHP) and the axonal conduction velocity. All of Anc and 65% of med. T motoneurons were classified into slow motoneurons. On the other hand, 35% of med. T, 84% of lo. T and la. T motoneurons were classified into fast motoneurons. About 70% of motoneurons innervating Bi and wrist controlling muscles (dR, M and U) were classified into fast motoneurons and the rest into slow motoneurons. Repetitive stimulation of the pericruciate cortex produced predominantly inhibitory PSPs (I-type) or mixed PSPs (M-type) in 67% of Anc and 56% of slow triceps motoneurons, while it evoked predominantly excitatory PSPs (E-type) in 97% of fast triceps motoneurons. Thus, patterns of PSPs in elbow extensor (Anc and triceps) motoneurons produced by cortical stimulation were in general excitatory to fast and inhibitory to slow motoneurons, similarly to the case in hindlimb motoneurons. In dR, M and U motoneurons, cortical stimulation produced E-type (84%) or M-type (16%) PSPs in fast motoneurons, while it evoked M-type (55%) or E-type (45%) PSPs in slow motoneurons. Patterns of PSPs in these motoneurons produced by cortical stimulation had a tendency similar to the case in triceps motoneurons; excitatory components of PSPs were predominant in fast motoneurons and inhibitory components were mainly observed in slow motoneurons. In most fast and slow Bi motoneurons, E-type or M-type PSPs were observed when the precruciate area was stimulated. With post-cruciate stimulation, however, excitatory components became smaller and inhibitory components were marked and changes in the pattern of PSPs were observed in 37% of motoneurons, as compared with precruciate stimulation. Thus, in Bi motoneurons, the qualitative difference of patterns of PSPs evoked by cortical stimulation in fast and slow motoneurons, as observed definitely in triceps motoneurons described avobe, was not clearly recognized.

Action Potentials↗

Fast spin-echo imaging of the neck: comparison with conventional spin-echo, utility of fat suppression, and evaluation of tissue contrast characteristics.

PURPOSE: To determine whether fast spin-echo sequences could replace conventional spin-echo methods in the evaluation of head and neck neoplasms and associated adenopathy and to evaluate differences in tissue contrast characteristics between conventional spin-echo and fast spin-echo examinations of head and neck disease. METHODS: Twenty-seven patients with squamous cell carcinoma were imaged on a 1.5-T imager with both conventional spin-echo and fast spin-echo sequences with identical section thickness and position. Twenty-one of the 27 fast spin-echo studies were performed with frequency-selective fat suppression. Three radiologists independently evaluated the images using a five-point scale to compare primary lesion margin definition and conspicuity, lymph node margin definition and conspicuity, gross motion artifact, and flow artifact. Quantitative percent contrast and contrast-to-noise ratios were calculated and compared in 7 cases with fat-suppressed fast spin-echo. RESULTS: Fast spin-echo was preferred by all three readers for lesion margin conspicuity and lymph node conspicuity. Gross motion and flow artifact demonstrated trends toward reader preference for fast spin-echo. Quantitative contrast values for fast spin-echo were significantly greater than those for conventional spin-echo. CONCLUSIONS: Fast spin-echo with fat suppression can replace conventional spin-echo at a time savings of more than 50% and improves tissue contrast and the conspicuity and definition of margins for primary lesions and lymph nodes. Fat-suppression heterogeneity remains the major limitation of this technique. Thus, careful attention to fat-suppression failure and unwanted water saturation is essential.

Adipose Tissue↗

Sarcoplasmic reticulum fast CA(2+)-pump and myosin heavy chain expression in extraocular muscles.

PURPOSE: To investigate the distribution of the sarcoplasmic reticulum fast-twitch Ca(2+)-pump (Ca(2+)-adenosine triphosphatase [Ca(2+)-ATPase]) in extraocular muscle fiber populations of the rat and rabbit, using fast Ca(2+)-adenosine triphosphatase-specific monoclonal antibodies. METHODS: Adult female rats and rabbits were killed with overdose of sodium pentobarbital, and the extraocular muscles were rapidly frozen in isopentane cooled with liquid nitrogen. Serial transverse frozen sections were cut and labeled respectively with monoclonal antibodies specific for various isoforms of myosin heavy chain, and monoclonal antibodies specific for the fast isoform of the sarcoplasmic reticulum Ca(2+)-ATPase. RESULTS AND CONCLUSIONS: In rat extraocular muscle, fast type 2 myosin heavy chain and fast Ca(2+)-ATPase are coexpressed in both singly- and multiply-innervated fibers of the orbital layer near the endplate band, but distally both proteins drop out entirely, indicating that the contraction and relaxation properties of orbital fibers vary along their length. Also, fast Ca(2+)-ATPase persists more distally in orbital fibers than does type 2 myosin heavy chain, suggesting that their joint expression is not directly coregulated. This conclusion is reinforced in rabbit, whose orbital multiply-innervated fibers express fast Ca(2+)-ATPase but do not express type 2 myosin heavy chain at all. Fast Ca(2+)-ATPase is expressed in all global layer fast-twitch singly-innervated fibers, but not in global multiply-innervated fibers. In light of known differences in myosin heavy chain expression, the two populations of multiply-innervated fibers must differ with respect to both contractility and relaxation.

Animals↗

Synchronization of fast (30-40 Hz) spontaneous cortical rhythms during brain activation.

We investigated the synchronization of fast spontaneous oscillations (mainly 30-40 Hz) in anesthetized and behaving cats by means of simultaneous extra- and intracellular recordings from multiple neocortical areas. Fast Fourier transforms, auto- and cross-correlations, and spike- or wave-triggered averages were used to determine the frequency and temporal coherence of fast oscillations that outlasted the stimulation of ascending activating systems or that occurred naturally during behavioral states of waking and rapid eye movement (REM) sleep but also appeared during the depolarizing phases of slow sleep oscillations. In 90% of microelectrode tracks, the fast oscillations did not show field reversal at any depth of the cortex and were not observable in the underlying white matter. The negative field potentials of the fast oscillations were associated at all depths with neuronal firing. This field potential property of fast oscillations was in sharp contrast to the reversal of slow sleep oscillation or evoked potentials at depths of 0.25-0.5 mm. The coherence of fast spontaneous rhythms was spatially limited, being confined within a cortical column and among closely located neocortical sites, in contrast to the long-range synchronization of slow sleep rhythms. Depolarizing current pulses elicited spike-bursts (200-400 Hz) recurring at a frequency of 30-40 Hz. Our experiments demonstrate that the conventional notion of a totally desynchronized cortical activity upon arousal should be revised as fast rhythms are enhanced and synchronized within intracortical networks during brain activation. Spontaneously occurring, subthreshold membrane potential depolarizing oscillations may bias cortical and thalamic neurons to respond synchronously, at fast frequencies, to relevant stimuli in the wake state or to internally generated drives in REM sleep.

Animals↗

Synchronization of fast (30-40 Hz) spontaneous oscillations in intrathalamic and thalamocortical networks.

The synchronization of fast (mainly 30 to 40 Hz) oscillations in intrathalamic and thalamocortical (TC) networks of cat was studied under ketamine-xylazine anesthesia and in behaving animals by means of field potential, extra- and intracellular recordings from multiple sites in the thalamic reticular (RE) nucleus, dorsal (sensory, motor, and intralaminar) thalamic nuclei, and related neocortical areas. Far from being restricted to tonically activated behavioral states, the fast oscillations also appeared during resting sleep and deep anesthesia, when they occurred over the depolarizing component of the slow (<1 Hz) oscillation and were suppressed during the prolonged hyper-polarizations of RE, TC, and cortical neurons. The synchronization of fast rhythms among different thalamic foci was robust. Fast rhythmic cortical waves and subthreshold depolarizing potentials in TC neurons were highly coherent; however, the synchronization of the fast oscillation required recordings from reciprocally related neocortical and thalamic foci, as identified by monosynaptic responses in both directions. The short-range spatial confinement of coherent fast rhythms contrasted with the large-scale synchronization of low-frequency sleep rhythms. Transient fast rhythms, appearing over the depolarizing envelope of the slow sleep oscillation, became sustained when brain activation was elicited by stimulation of mesopontine cholinergic nuclei or during brain-active behavioral states in chronic experiments. These data demonstrate that fast rhythms are part of the background electrical activity of the brain and that desynchronization, used to designate brain-active states, is an erroneous term inasmuch as the fast oscillations are synchronized not only in intracortical but also in intrathalamic and TC networks.

Action Potentials↗

Time-dependent change in fast pathway refractoriness after slow pathway ablation in atrioventricular node reentrant tachycardia. Mansfield Polaris Investigators.

OBJECTIVE: To determine the time course of change in fast pathway refractoriness after slow pathway ablation. BACKGROUND: Antegrade fast pathway refractoriness has been observed to shorten in patients undergoing slow pathway ablation for atrioventricular (AV) node reentrant tachycardia. The time course and mechanism of this observation have not been explained. METHODS: Twenty-eight patients with AV node reentrant tachycardia and dual AV node pathways undergoing slow pathway ablation had the fast pathway effective refractory period (ERP) assessed immediately before, and at 0, 15, 30 and 45 mins after slow pathway ablation (Group 1). Twenty-five additional patients with AV node reentry and dual pathways involved in a multicentre protocol evaluating the Mansfield Polaris LE catheter underwent assessment of fast pathway refractoriness before and after slow pathway ablation, and at a routine three-month follow-up electrophysiology study (Group 2). RESULTS: In Group 1, antegrade fast pathway ERP fell from 394 ms before ablation to 334 ms immediately after slow pathway ablation, increased to 348 ms within 15 mins and was 353 ms at 45 mins (ANOVA P < 0.001). Retrograde fast pathway ERP fell from 325 ms before ablation to 294 ms at 45 mins (P = 0.02). In Group 2, antegrade fast pathway ERP fell from 390 ms before ablation to 337 ms after ablation, and rose to 362 ms at three months (P = 0.01). Retrograde fast pathway ERP also fell from 347 ms to 319 ms after ablation (P = 0.01), and remained unchanged at three months. CONCLUSION: Slow pathway ablation results in an immediate and sustained change in antegrade and retrograde first pathway refractoriness. There are immediate reversible and long term nonreversible components to this phenomenon. The latter finding may be related to loss of electrotonic inhibition of the fast pathway by the slow pathway.

Adult↗

Characteristics of foveating and defoveating fast phases in latent nystagmus.

PURPOSE: Under certain conditions, the fast phases of latent/manifest latent nystagmus (LMLN) can defoveate the target of interest instead of foveating it, as was thought to be their only function. LMLN fast phases in the waveforms from four subjects were studied with the goals of better understanding their characteristics and determining what triggers both foveating and defoveating fast phases. METHODS: Eye movement records were made using both the scleral search coil and infrared methods. Relationships of fast-phase sizes with slow-phase positions and velocities before and after fast phases were analyzed, as were relationships of saccade size with peak velocity and duration. RESULTS. The data showed that LMLN with defoveating fast phases occurred in the presence of higher slow-phase velocities. Also, larger saccade sizes corresponded to larger presaccadic and postsaccadic slow-phase velocities. The peak velocities and durations of LMLN fast phases were in the same ranges as normal saccades. CONCLUSIONS: Defoveating fast phases with decreasing-velocity slow phases may be the result of the addition of saccadic pulses to linear slow phases. Mechanisms are suggested to explain the switch from foveating to defoveating fast phases in LMLN.

Electronystagmography↗

Effects of moderate alcohol intake on fasting insulin and glucose concentrations and insulin sensitivity in postmenopausal women: a randomized controlled trial.

CONTEXT: Epidemiologic data demonstrate that moderate alcohol intake is associated with improved insulin sensitivity in nondiabetic individuals. No controlled-diet studies have addressed the effects of daily moderate alcohol consumption on fasting insulin and glucose concentrations and insulin sensitivity. OBJECTIVE: To determine whether daily consumption of low to moderate amounts of alcohol influences fasting insulin and glucose concentrations and insulin sensitivity in nondiabetic postmenopausal women. DESIGN, SETTING, AND PARTICIPANTS: Randomized controlled crossover trial of 63 healthy postmenopausal women, conducted at a clinical research center in Maryland between 1998 and 1999. INTERVENTIONS: Participants were randomly assigned to consume 0, 15, or 30 g/d of alcohol for 8 weeks each as part of a controlled diet. All foods and beverages were provided during the intervention. An isocaloric beverage was provided in the 0-g/d arm. Energy intake was adjusted to maintain constant body weight. MAIN OUTCOME MEASURES: Fasting insulin, triglyceride, and glucose concentrations, measured at the end of each dietary period; insulin sensitivity, estimated with a published index of glucose disposal rate corrected for fat-free mass based on fasting insulin and fasting triglyceride concentrations, compared among treatments with a mixed-model analysis of variance. RESULTS: A complete set of plasma samples was collected and analyzed for 51 women who completed all diet treatments. Consumption of 30 g/d of alcohol compared with 0 g/d reduced fasting insulin concentration by 19.2% (P =.004) and triglyceride concentration by 10.3% (P =.001), and increased insulin sensitivity by 7.2% (P =.002). Normal-weight, overweight, and obese individuals responded similarly. Only fasting triglyceride concentration was significantly reduced when comparing 0 and 15 g/d of alcohol (7.8%; P =.03), and no difference was found between consumption of 15 and 30 g/d of alcohol; however, there was a significant linear trend (P =.001). Fasting glucose concentrations were not different across treatments. CONCLUSIONS: Consumption of 30 g/d of alcohol (2 drinks per day) has beneficial effects on insulin and triglyceride concentrations and insulin sensitivity in nondiabetic postmenopausal women.

Aged↗

Proportions of slow-twitch and fast-twitch extrafusal fibers in receptive fields of tendon organs in chicken leg muscles.

Golgi tendon organs are mechanoreceptors that monitor the contractile force produced by motor units. Receptors are most responsive to contractions of extrafusal muscle fibers that terminate closest to them and on them. Three anterior and four posterior chicken leg muscles were examined. Proportions of immunohistochemically identified slow-twitch extrafusal fibers and fast-twitch extrafusal fibers were calculated for 374 tendon organ receptive fields. Tendon organs were observed in muscle regions occupied either by slow-twitch fibers or fast-twitch fibers only, but most were found in regions that contained both slow-twitch and fast-twitch extrafusal fibers. The frequency with which each fiber type occurred near tendon organs approached the frequency with which it occurred in more inclusive regions. In receptive fields with mixed fiber populations, fast-twitch fibers were the predominant type, especially in the anterior leg muscles. Distribution patterns of extrafusal fiber types adjacent to and farther removed from tendon organs suggest that afferent discharges from tendon organs are by and large unbiased measures of the contractile activity of the extrafusal fiber population of the muscle portion in which the tendon organs are located. In mixed muscle regions, slow-twitch fibers and fast-twitch fibers attach on given tendon organs, enabling them to monitor forces produced by slow motor units and by fast motor units. Most tendon organs are situated in mixed extrafusal fiber fields with high fast-twitch fiber content, indicating that in chicken leg muscles sensory feedback from tendon organs is largely one from fast motor units.

Animals↗

Preoperative fasting for preventing perioperative complications in children.

BACKGROUND: Children, like adults, are required to fast before general anaesthesia with the aim of reducing the volume and acidity of their stomach contents. It is thought that fasting reduces the risk of regurgitation and aspiration of gastric contents during surgery. Recent developments have encouraged a shift from the standard 'nil-by-mouth-from-midnight' fasting policy to more relaxed regimens. Practice has been slow to change due to questions relating to the duration of a total fast, the type and amount of intake permitted. OBJECTIVES: To systematically assess the effects of different fasting regimens (duration, type and volume of permitted intake) and the impact on perioperative complications and patient wellbeing (aspiration, regurgitation, related morbidity, thirst, hunger, pain, comfort, behaviour, nausea and vomiting) in children. SEARCH STRATEGY: We searched Cochrane Wounds Group Specialised Register, the Cochrane Central Register of Controlled Trials, MEDLINE, CINAHL, the National Research Register, relevant conference proceedings and article reference lists and contacted experts. SELECTION CRITERIA: Randomised and quasi randomised controlled trials of preoperative fasting regimens for children were identified. DATA COLLECTION AND ANALYSIS: Data extraction and trial quality assessment was conducted independently by two authors. Trial authors were contacted for additional information including adverse events. MAIN RESULTS: Forty-three randomised controlled comparisons (from 23 trials) involving 2350 children considered to be at normal risk of regurgitation or aspiration during anaesthesia. Only one incidence of aspiration and regurgitation was reported. Children permitted fluids up to 120 minutes preoperatively were not found to experience higher gastric volumes or lower gastric pH values than those who fasted. The children permitted fluids were also less thirsty and hungry, better behaved and more comfortable than those who fasted. Clear fluids preoperatively did not result in a clinically important difference in the children's gastric volume or pH. Evidence relating to the preoperative intake of milk was sparse. The volume of fluid permitted during the preoperative period did not appear to impact on children's intraoperative gastric volume or pH contents. AUTHORS' CONCLUSIONS: There is no evidence that children who are not permitted oral fluids for more than six hours preoperatively benefit in terms of intraoperative gastric volume and pH over children permitted unlimited fluids up to two hours preoperatively. Children permitted fluids have a more comfortable preoperative experience in terms of thirst and hunger. This evidence applies only to children who are considered to be at normal risk of aspiration/regurgitation during anaesthesia.

Adolescent↗

Physiological adaptations to fasting in an actively wintering canid, the Arctic blue fox (Alopex lagopus).

This study investigated the physiological adaptations to fasting using the farmed blue fox (Alopex lagopus) as a model for the endangered wild arctic fox. Sixteen blue foxes were fed throughout the winter and 32 blue foxes were fasted for 22 d in Nov-Dec 2002. Half of the fasted blue foxes were food-deprived again for 22 d in Jan-Feb 2003. The farmed blue fox lost weight at a slower rate (0.97-1.02% body mass d(-1)) than observed previously in the arctic fox, possibly due to its higher initial body fat content. The animals experienced occasional fasting-induced hypoglycaemia, but their locomotor activity was not affected. The plasma triacylglycerol and glycerol concentrations were elevated during phase II of fasting indicating stimulated lipolysis, probably induced by the high growth hormone concentrations. The total cholesterol, HDL- and LDL-cholesterol, urea, uric acid and total protein levels and the urea:creatinine ratio decreased during fasting. Although the plasma levels of some essential amino acids increased, the blue foxes did not enter phase III of starvation characterized by stimulated proteolysis during either of the 22-d fasting procedures. Instead of excessive protein catabolism, it is liver dysfunction, indicated by the increased plasma bilirubin levels and alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase activities, that may limit the duration of fasting in the species.

Acclimatization↗

Hyperthermia and increased physical activity in the fasting American mink Mustela Vison.

The aim of this study was to investigate the thermoregulatory adaptations to fasting in a medium-sized mustelid with a high metabolic rate and energetic requirements. Sixteen farm-bred female American minks, Mustela vison, were divided into a fed control group and an experimental group fasted for 5 days. The deep body temperature (T(b)) of the minks was registered at 10 min intervals with intraabdominal thermosensitive loggers and the locomotor activity was videotaped continuously for 5 days during the fasting procedure. The T(b) of the fasted animals increased during the first day of fasting and decreased during the second day. After 3-4 days of fasting, the levels of physical activity and T(b) of the fasted minks increased above the levels of the fed animals. Significant increases in these parameters were observed at the beginning of the working day on the farm, during the feeding of the fed animals and around midnight. It is concluded that the mink differs from previously studied homeotherms in thermoregulatory and behavioral responses to fasting probably due to its high energy requirements and predatory success.

Animals↗

Factors affecting fasting serum C-peptide levels in Micronesians: comparison with a Caucasoid population.

Fasting serum C-peptide immunoreactivity was determined on Nauruans, a Micronesian population with a high prevalence of diabetes. In Micronesian subjects neither age nor gender had a significant effect on fasting serum C-peptide. In non-diabetic subjects, as has been shown previously for Caucasiod subjects, both obesity and fasting plasma glucose levels were determinants of fasting serum C-peptide. Obesity was the major determinant. Taken overall, mean fasting serum C-peptide increased then possibly fell in subjects grouped by increasing 2-h post-glucose plasma glucose levels. Mean fasting serum C-peptide in newly-diagnosed diabetic subjects was greater than that in non-diabetic subjects with a similar degree of obesity, supporting the concept that the transition to diabetes may be associated with an increase in insulin resistance. The data for non-diabetic subjects were compared with serum C-peptide measured in the same laboratory on samples from a Caucasoid population in Busselton, Western Australia. There was no difference in fasting serum C-peptide level between Micronesian and Caucasoid subjects approximately matched for obesity and fasting plasma glucose levels.

Adult↗

Calcium, zinc and other elements in islet and exocrine tissue of the rat pancreas as measured by histochemical methods and electron-probe micro-analysis, Effects of fasting and tolbutamide.

Fasting for 24 or 72 h causes a strong decrease of pancreatic islet calcium content as detected by glyoxal-bis-(2-hydroxyanil), (GBHA). There is strong evidence that GBHA only detects ionized calcium and not total calcium (Wolters et al., 1979). Fasting does not influence the zinc content as detected by dithizone (DZN), and aldehyde-fuchsin (AF) staining intensity is only slightly decreased. After degranulation of islets by tolbutamide (which reduced the insulin content of the pancreas to 10% of the control value) the staining intensities of GBHA, DZN and AF were strongly depressed. Calcium (as well as other elements) were also measured by electron-probe micro-analysis (EPMA). It appeared that 24 or 72 h of fasting did neither affect the total content of Ca nor of Na, P, S, and K of the islets significantly. In exocrine tissue the Ca content increased gradually as a result of fasting. Thus, after 72 h of fasting the Ca content was 25% higher than in fed controls. On the other hand after 72 h of fasting the K content appeared to be decreased. EPMA revealed that after degranulation of islets the Ca content decreased markedly (35%). S appeared to be decreased by only 14%, whereas the content of the other elements was not changed. The results show that GBHA-detectable Ca is only a part of EPMA-detectable Ca. The GBHA-Ca "pool" which contains ionized Ca, is subjected to changes when the animals are fasted, the total Ca content as measured by EPMA does not change. Thus, at least two distinguishable pools of Ca exist within the islets (GBHA-detectable and not-GBHA-detectable). It is suggested that as a result of fasting Ca passes from one pool to another.

Animals↗