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PubMed2001

Mountaineering fatalities in Mt Cook National Park.

AIM: To estimate the risk of death associated with mountaineering in the Mt Cook National Park (MCNP), and to describe some characteristics of the fatal events. METHODS: Fatality data, including coroners' files, were obtained from the Mountain Safety Council and the Department of Conservation, Mt Cook field office. Data on occupancy of mountain huts were used to estimate rates. RESULTS: 33 deaths occurred among climbers using the alpine huts studied, over a period in which climbers spent 52 906 nights in the huts. The overall fatality rate was 0.62/1000 hut nights. This is estimated to equate with a fatality rate of 1.87/1000 climbing days. Fatality risk estimates varied more than 50-fold between huts serving the highest risk (6.5/1000 days) and lowest risk (0.3/1000 days) climbing areas within the MCNP. CONCLUSION: The risk of death associated with mountaineering in MCNP varies greatly with the difficulty and seriousness of the climbing undertaken. The risk associated with the more serious climbing in MCNP is very similar to that reported for climbers on expeditions to extreme altitude. Even the lower risk estimates are very high when compared with those for most other recreational activities.

Accidents↗
PubMedDate not supplied

Method of determination of appropriate heat treatment of animal meal by immunoassay developed for detection of cooked beef: interlaboratory study.

An interlaboratory trial was conducted for the validation of an enzyme-linked immunosorbent assay (ELISA) method for determination of appropriate heat treatment of animal meal. A commercially available ELISA test kit developed for the identification of beef in cooked food was used in the study. Twelve laboratories from 7 European countries examined 2 different analytical protocols to establish the most appropriate analytical method. Three different samples were used, 2 animal waste materials sterilized at 129 and 134 degrees C (wet conditions), respectively, and a meat and bone meal material processed at dry conditions (maximum temperature, 140 degrees C). Statistical evaluation applying t-statistics showed that the animal meal treated according to European legislation (>133 degrees C) was clearly distinguishable from the 2 other test materials at a 99% confidence level using both analytical protocols. This method can be considered as a complementary test to the immunoassay developed for the detection of pork in cooked food that is already applied in routine analysis for the surveillance of rendering plants.

Animal Feed↗
PubMed2002

Homestyle quick-cooking rice meal products.

The study developed processes for quick-cooking rice meal products (QCRMP) paella and bringhe originally based on homestyle recipes. These QCRMPs consisted of basal quick-cooking waxy Malagkit Sungsong and non-waxy irrigated rice (IR) 42 Philippine rice cultivars, dehydrated vegetables, textured vegetable proteins (TVPs) as meat analogs, and seasonings. The rehydrated QCRMP paella and bringhe were found to be more acceptable than their conventionally prepared counterparts at 5% level of significance based on results of sensory acceptability evaluation by 50 consumer panelists. Improvement of the sensory attributes of the QCRMPs was ascribed to the use of alternate concentrates and powdered ingredients which imparted more intense flavors to the developed products than their fresh counterparts.

Developing Countries↗
PubMed2002

[Effect of high hydrostatic pressure on water imbibition, cooking times and microstructure of Phaseolus vulgaris].

The effect of high hydrostatic pressure (HHP) on water imbibition, cooking times, and microstructure of cotyledons and seed coats of black beans was evaluated. High pressure treatments at 275, 410, 550 or 690 MPa and 25 degrees C for 5 min increased the rate of water imbibition of black beans. The saturation degree of HHP treated black beans was reached 50% faster than the saturation degree of untreated black beans. High hydrostatic pressure treatments reduced the cooking times (CT) of unsoaked black beans by 25% to 39%, similar to black beans soaked for 3 h. The microstructure of HHP treated beans exhibited protein aggregation in cell protoplasm and swelling of cell walls and starch granules. Seed coats of HHP treated black beans were less smooth than seed coats of untreated black beans. The swelling of palisade, hourglass, and parenchyma cells was also observed in HHP treated black beans such as soaked beans. High hydrostatic treatment is an alternative for reducing the preparation long times of Phaseolus vulgaris.

Absorption↗
PubMed2002

[Placement of a double-lumen tube using a 6 mm diameter fibro-bronchoscope and a Cook exchange catheter in a patient with unforeseen tracheal intubation difficulty].

The trachea of a 74-year-old man undergoing left pneumonectomy could not be intubated in two attempts after induction of anesthesia with midazolam, fentanyl, propofol and rocuronium. Difficult intubation had not been foreseen, but inspection through the laryngoscope revealed Cormack and Lehane grade IV conditions. Because a small-caliber fiberoptic bronchoscope was unavailable for intubation with a double-lumen endobronchial tube, we inserted a No. 9 orotracheal tube with a 6 mm bronchoscope as far as the left main bronchus. Intubation was aided by a universal adaptor for fiberoptics with a face mask and a Williams cannula. We were then able to ventilate the patient manually with 100% oxygen during bronchoscopy. As selective ventilation was required during surgery, a No. 11 Cook-type airway exchange catheter was inserted into the left main bronchus, the tracheal tube was removed, and was used to guide a No. 39F left double-lumen endobronchial tube through the bronchus. Insertion was uncomplicated and selective ventilation was satisfactory. The technique described is a new application for the Cook exchange catheter that allows selective bronchial in difficult cases when a small-caliber fiberoptic bronchoscope is unavailable.

Aged↗
PubMedDate not supplied

[Quality assurance of cooked food in conventional hospital food service].

The aim of this study was to evaluate the effectiveness of the hospital food service of the Azienda in preserving microbiological quality and temperature of cooked foods during the distribution to the different wards of the hospital. During three years, microbiological parameters and temperatures were monitored in the central kitchen before distribution (T0), in the first (T1) and in the last ward (T2) of the distribution route during a three-year period for a total of 337 analytical determinations. Temperature values before distribution improved during the study period, whereas the capacity of keeping adequate temperature values during the distribution decreased over time, in spite of the introduction of a new technology after the first year of the study. No significant deteriorations of the microbiological quality of the cooked foods were detected. The results of the study clearly indicate that an integrated approach involving new technology, work organization and personnel training is needed in preserving food quality in hospitals.

Food Handling↗
PubMedDate not supplied

Liquid chromatography/tandem mass spectrometry analysis of chloramphenicol in cooked crab meat.

A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method is described for the extraction, cleanup, determination, and confirmation of chloramphenicol (CAP) in cooked crab meat. The method involves pulverization of cooked crab meat with dry ice; extraction of the CAP into ethyl acetate (EtOAc); evaporation (by N2) of the EtOAc; addition of methanol, aqueous NaCl, and heptane; extraction of the lipids into the heptane, followed by extraction of the aqueous phase with EtOAc; evaporation (by N2) of the EtOAc; dissolution into methanol-water; filtration; and separation/detection/confirmation using LC/MS/MS. Crab meat was fortified at 0.25, 0.50, and 1.0 ng/g (ppb) chloramphenicol. Average absolute recoveries were 67, 84, and 86%, respectively, with relative standard deviation values all less than 1%. Four daughter ions (m/z 152, 176, 194, and 257) were monitored off the m/z 321 precursor ion. Determination was based on a standard curve using the peak areas of the m/z 152 daughter ion (the base peak) for standard solutions equivalent to 0.10, 0.20, 0.50, and 1.0 ppb in tissue (made with control crab extract). A set of 6 matrix controls (unfortified crab meat) was also analyzed, in which no chloramphenicol was detected. For identification purposes, the ion ratios (of each daughter ion versus the base daughter ion) of the fortified crab versus those of the chloramphenicol standards agreed within 10% (relative) at fortified chloramphenicol concentrations of 0.25-1.0 ppb.

Acetates↗
PubMed2006

The career aspirations and location intentions of James Cook University's first cohort of medical students: a longitudinal study at course entry and graduation.

INTRODUCTION: James Cook University's School of Medicine enrolled its first cohort of 64 students in 2000. The School was established with the aim of increasing the number of medical graduates who understand rural, remote, Indigenous and tropical health issues and who would subsequently choose rural (non-metropolitan) practice. In December 2005, 58 students graduated the course. Several students left the cohort (either left the course or repeated earlier years of the course). In the early years, students who left the course were replaced by accelerated entry students. METHOD: A student-initiated survey, involving questionnaire and interview components, conducted in 2001 sought information on students' career aspirations and practice location intentions, as well as why students had chosen medicine and the James Cook University course in particular. A brief follow-up survey focused on career aspirations, location of internship and longer term practice location intentions was conducted in October 2005 (2 months before the students graduated). In each instance, participation was anonymous and no identifying information was recorded. Accelerated entry students' responses to the 2005 survey were included. RESULTS: In 2001, 57 (89%) of the 64 students participated in the study; and in 2005, 50 (86%) of the 58 graduating students responded to the questionnaire survey. Overall, the demographic composition of the 'cohort' changed little. In 2001, 66% of respondents intended to practice in non-metropolitan areas, with 64% so intending at graduation. This was reinforced by 64% choosing internship positions in non-metropolitan areas of Queensland; 56% in north Queensland. Career aspirations changed appreciably between 2001 and 2005: the number of undecided students had halved, the numbers interested in general practice had reduced by one-third, the numbers considering surgery had reduced to one-third, and none was considering paediatrics at exit. Conversely, the number considering emergency medicine had almost doubled and more than doubled for obstetrics and gynaecology. DISCUSSION: Given that the majority of intern positions in Queensland are in the south-east of the state, it is noteworthy that two-thirds of students sought and accepted posts elsewhere, predominantly in northern Queensland. Changes in career aspirations reflect two issues. First, respondents' 2005 recollections of what their aspirations were in 2001 varied, reinforcing the value of the longitudinal process. Second, the change of intentions across time possibly reflects exposure to various disciplines and perhaps positive/negative experiences during clinical rotations in the final years of the course. The consistency across time with respect to intention to practice in rural (non-metropolitan) areas suggests that most respondents had eyes on rural practice from early in the course. The findings are in line with other studies that have demonstrated two important factors influence clinicians' decisions to take up non-metropolitan practice: (1) non-metropolitan background; and (ii) substantial and meaningful exposure to non-metropolitan practice during the course. CONCLUSION: The findings support the School's contention and that of others around the world that medical education undertaken in non-metropolitan settings is the best vehicle for increasing the rural medical workforce. This study provides support for the development of regional medical schools that focus on local recruitment and health care need issues.

Adolescent↗
PubMed1991

[Combined methods of dialysis, cooked meat medium enrichment and laboratory animal toxicity for screening Clostridium botulinum spores in honey and infant food].

One hundred and fifty two samples of honey were purchased from apiaries and markets in Taiwan, meanwhile fifty samples of infant food including powders of infant milk, wheat, rice and commercial mixed cereals, as well as juice had been bought from supermarkets in Taipei city from July 1988 through April 1989. The samples were used for detecting the presence of Clostridium botulinum spores. Honey samples were prepared by dialysis to obtain the bacterial spores; however, infant food had been innoculated directly into the cooked meat medium. The suspicious isolated colonies were identified by Vitek automatic microbial identification system. The supernatants of cooked meat medium enrichment were performed for typing botulinum toxins via the laboratory animal toxicity test. The results showed that two samples of honey contained the spores of C. botulinum type B, but none in samples of infant food. The specific response of clinical signs in mice, after being administered the supernatant of suspected cultural broth, were also observed and described in this article.

Animals↗
PubMed1990

Kinetic investigation of the aqueous stability and antitumor activity of a hydrosoluble diaryltriazene, AVIS, related to the antimetastatic agent DM-COOK.

The aim of this study is to investigate the hydrolysis of 1,3-di(p-carboxyphenyl)triazene dipotassium salt, AVIS (1), over a pH range of 2.60-8.50. This compound decomposes into p-aminobenzoic acid and the corresponding diazonium cation with no formation of alkylcarbo cations; the same compounds are formed from hydrolyses of DM-COOK (2), an antimetastatic agent, and of its possible demethylated metabolite, MM-COOK (3), a chemical xenogenization inducer. In these latter cases, however, a methylcarbo cation is formed. The pH dependence of the pseudo-first-order rate constants is intermediate between 2 and 3. Preliminary data on its toxicity and antitumor activity on both Lewis lung carcinoma and TLX5 lymphoma seem to indicate the essential role of alkylcarbo cation in mediating the antitumor action of aryldimethyltriazenes.

Animals↗
PubMedDate not supplied

Rapid headspace gas chromatographic method for assessment of oxidative stability of cooked chicken meat.

Experiments were conducted to determine the volatile compounds in cooked chicken meat using a static headspace gas chromatographic (GC) technique. Preheating conditions for samples in vials were tested at 70, 80, and 90 degrees C for 20-120 min at each temperature. The majority of the peaks increased in size as the temperature and time increased. Optimum conditions were established as preheating at 80 degrees C for 30 min followed by analysis on a packed column of 8% Poly MPE on Tenax GC with the temperature programmed from 50 to 200 degrees C at 10 degrees/min. Coefficients of variation for major peaks ranged from 8.3 to 14.7%. These results were compared with those obtained with a capillary column analysis of samples preheated at the same conditions. Cooked and stored chicken patties, pretreated with different levels of sodium tripolyphosphate, were analyzed by the thiobarbituric acid (TBA) method and the headspace GC technique. Significant positive correlations were obtained between TBA numbers and the areas of 3 major peaks of the headspace profiles, indicating the applicability of the rapid headspace GC method for the determination of oxidative changes in chicken meat.

Animals↗
PubMed1978

Improved microbiological assay for penicillin residues in tissues and stability of residues under cooking procedures.

The microbiological assay for penicillin residues was improved by using centrifugation to remove physical barriers to diffusion, a small buffer/meat extraction ratio, and a more sensitive 2-layer assay system. Recoveries from muscle, kidney, and liver tissues ranged between 70.1 and 86.7% with measurable levels of 0.03--0.05 unit/g. By comparison, the Food and Drug-suggested methodology yielded recoveries of 45.9--54.0% and levels of detectability of 0.08--0.10 unit/g. Cooking of hamburger, steaks, and port chops indicated that procaine penicillin withstood cooking conditions, and significant levels of the original activity remained.

Animals↗
PubMed1983

Characterization of mutagenic fractions in beef extract and in cooked ground beef. Use of blue-cotton for efficient extraction.

Mutagenic components in commercial beef extract and in cooked ground beef were adsorbed from their aqueous solutions on cotton bearing covalently linked trisulfo-copper-phthalocyanine residues (blue-cotton). By repeating the adsorption and elution, efficient concentration of the mutagenic components with a satisfactory overall recovery was achieved. Carboxymethyl cellulose column chromatography was found to be an excellent means to separate 2-amino-3,8-dimethylimidazo-[4,5-f]quinoxaline (MeIQx) from 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), two strong mutagens that have previously been found in heated beef samples. Chromatography of the mutagenic components of beef extract on this column gave two mutagenic fractions which corresponded to MeIQx and IQ in elution profile. In reversed-phase high performance liquid chromatography, the major active component of the MeIQx fraction and that of the IQ fraction behaved identically with standard samples of MeIQx and IQ, respectively. The contents of these mutagens in a sample of Difco beef extract were estimated at 200-300 ng of MeIQx and 20-40 ng of IQ per gram. By the same fractionation procedures, mutagenic substances in the cooked beef were fractionated into MeIQx-type and IQ-type components. The activity distribution among these two fractions was similar to that found for beef extract.

Adsorption↗
PubMed1982

Antibiotic resistance and R plasmids in coliforms isolated from some Malaysian cooked foods.

Forty samples of Malaysian cooked foods were examined for the presence of antibiotic-resistant coliforms and R plasmids. Twenty seven (68%) of the foods had antibiotic-resistant coliforms and 5 (13%) had R plasmids. Nineteen samples (48%) had total bacterial counts over 10(6) per gm and in 5 samples, no coliforms were detected. Our findings show that cooked food may be one possible way by which R plasmids are spread. The control of the spread of R plasmids is discussed.

Anti-Bacterial Agents↗
PubMedDate not supplied

[Investigation of the presence of Staphylococcus aureus in cooked ham using 3 methods (preliminary study)].

Fifty-one cooked ham samples were analized by three methods for investigation of Staphylococcus aureus; Modified Van Doorne and American Public Health Association; The Official Mexican Method. The first two are enrichment methods and in all three a comparison between Baird Parker agar and salt milk agar was done. The modified Van Doorne technique was the best for isolation of S. aureus from cooked ham. In Baird Parker agar it was possible to demonstrate the presence of S. aureus in all positive samples. The study shows the importance of using Baird Parker broth as an enrichment medium for the isolation of S. aureus from products in which a thermal treatment and addition of salts as sodium chloride and nitrites, inhibit the growth of this microorganism.

Animals↗
PubMed1996

Texture evaluations of cooked, diced broiler breast samples by sensory and mechanical methods.

The texture of cooked chicken breast is usually determined on intact pieces or strips that are representative of the muscle. Researchers emphasize the importance of uniformity of sample size and orientation of fibers to the shearing blades to ensure consistent data from instruments such as shear devices. For sensory evaluations, sample pieces of chicken breasts presented to panelists are often sized as single 2-cm2 or several 1-cm2 pieces. In this study, two sensory and two mechanical procedures were evaluated for efficacy in discerning texture differences in 1-cm2 pieces of cooked chicken. Three postmortem deboning times (2, 6, and 24 h) provided a texture quality spectrum. Sensory characteristics were evaluated by descriptive analysis trained panel (DA-TP) and by category scales-untrained panel (CS-UP). Twenty-gram breast portions of 1-cm2 pieces were evaluated using a multi-blade Allo-Kramer (AK) shear blade. Warner-Bratzler (WB) shear values of intact 1.9-cm-wide strips were also recorded. Discriminant analysis classification based on CS-UP gave higher error rates (probabilities of misclassification) than classification based on DA-TP. The CS-UP error rates were 0.36 (tender) and 0.65 (juiciness). The error rate of classification by DA-TP was 0.21. Error rates for classification by mechanical devices were 0.30 for WB and 0.21 for AK. Sample size is a consideration in texture evaluation protocols. Smaller sample pieces appear to mask differences in initial chew characteristics and in juiciness that are due to deboning time.

Adult↗
PubMed1998

Syphilis screening among women arrestees at the Cook County Jail--Chicago, 1996.

Cook County (Chicago) Department of Corrections (i.e., Cook County Jail [CCJ]) is one of the largest jail systems in the United States, with a daily census of approximately 10,000 arrestees. CCJ contracts with Cermak Health Services (CHS) to provide medical services, including routine syphilis screening, to arrestees at CCJ. On January 6, 1996, the Chicago Department of Public Health (CDPH) STD/HIV Program, in collaboration with the CCJ, CHS, and CDC, implemented the Rapid Plasma Reagin (Stat RPR) Project at CCJ to improve syphilis identification and treatment rates among women. This report describes the project and provides data from the first year of operation. The findings indicate that Stat RPR yields a higher treatment rate for women arrestees than routine syphilis testing.

Adult↗