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PubMed2002

The impact of theoretical errors on velocity estimation and accuracy of duplex grading of carotid stenosis.

Two potential errors in velocity estimation, Doppler angle misalignment and intrinsic spectral broadening (ISB), were determined and used to correct recorded blood velocities obtained from 20 patients (38 bifurcations). The recorded and corrected velocities were used to grade stenoses of greater than 70% using two duplex classification schemes. The first scheme used a peak systolic velocity (PSV) of > 250 cm/s in the internal carotid artery (ICA), and the second a PSV ratio of > 3.4 (ICA PSV/common carotid artery PSV). The "gold standard" was digital subtraction angiography (DSA). The maximum error in velocity estimation due to Doppler angle misalignment was 33 cm/s, but this did not alter sensitivity of stenosis detection. ISB correction caused a reduction in PSV that decreased the sensitivity of the PSV scheme from 65% to 45%. The PSV ratio classification was not affected by ISB errors. Centres using a PSV criterion for grading stenosis should use a fixed Doppler angle and should establish velocity thresholds in-house.

Aged↗
PubMed1977

Cooperative unit size in the gel-liquid crystalline phase transition of dipalmitoyl phosphatidylcholine-water multilayers: an estimate from Raman spectroscopy.

The temperature dependence of the Raman spectral transitions assigned to the acyl chain C-C stretching modes of dipalmitoyl phosphatidylcholine was determined for the gel, phase transition and liquid crystalline states of the lipid multilayers. The van't Hoff enthalpy differences delta HVH between trans and gauche rotational isomers were obtained from the Raman spectral data for the temperature region characteristic of each bilayer state. An average size for the cooperative unit undergoing the chain melting process during the phase transition was estimated from the ratio of the appropriate van't Hoff enthalpy to an adjusted calorimetric enthalpy.

Binding Sites↗
PubMed2000

Ages of Late Spectral Type Vega-like Stars.

We have estimated the ages of eight late-type Vega-like stars by using standard age-dating methods for single late-type stars, e.g., location on the color-magnitude diagram, Li lambda6708 absorption, Ca ii H and K emission, X-ray luminosity, and stellar kinematic population. With the exception of the very unusual pre-main-sequence star system HD 98800, all the late-type Vega-like stars are the same age as the Hyades cluster (600-800 Myr) or older.

Journal Article↗
PubMed2004

Spectral quality assessment for high-throughput tandem mass spectrometry proteomics.

Current techniques in tandem mass spectrometric analyses of cellular protein contents often produce thousands to tens of thousands of spectra per experiment. This study introduces a new algorithm, named SPEQUAL, which is aimed at automated tandem mass spectral quality assessment. The quality of a given spectrum can be evaluated from three basic components: (i) charge state differentiation, (ii) total signal intensity, and (iii) signal-to-noise estimates. The differentiation between single and multiple precursor charge states (i) provides a binary score for a given spectrum. Components (ii) and (iii) provide partial scores which are subsequently summarized and multiplied by the first score. SPEQUAL was applied to over 10,000 data files derived from almost 3,000 tandem mass spectra, and the results (final cumulative scores) were manually verified. SPEQUAL's performance was determined to have high sensitivity and specificity and low error rates for both spectral quality estimates in general and precursor charge state differentiation in particular. Each of the partial scores is controlled by adjustable thresholds to fine-tune SPEQUAL's performance for different analysis pipelines and instrumentation. This spectral quality assessment tool is intended to act in an advisory role to the researcher, assisting in filtration of thousands of spectra typically produced by high throughput tandem mass spectrometric proteome analyses. Lastly, SPEQUAL was implemented as Java GUI-based and command-line-based interfaces freely available for both academic and industrial researchers.

Mass Spectrometry↗
PubMed2004

Customized spectral band analysis compared with conventional Fourier analysis of heart rate variability in neonates.

A customized filtering technique is introduced and compared with fast Fourier transformation (FFT) for analyzing heart rate variability (HRV) in neonates from short-term recordings. FFT is classically the most commonly used spectral technique to investigate cardiovascular fluctuations. FFT requires stability of the physiological signal within a 300 s time window that is usually analyzed in adults. Preterm infants, however, show characteristics of rapidly fluctuating heart rate and blood pressure due to an immature autonomic regulation, resulting in non-stationarity of these signals. Therefore neonatal studies use (half-overlapping or moving) windows of 64 s length within a recording time of 2-5 min. The proposed filtering technique performs a filtering operation in the frequency range of interest before calculating the spectrum, which allows it to perform an analysis of shorter periods of only 42 s. The frequency bands of interest are 0.04-0.15 Hz (low frequency, LF) and 0.4-1.5 Hz (high frequency, HF). Although conventional FFT analysis as well as the proposed alternative technique result in errors in the estimation of LF power, due to spectral leakage from the very low frequencies, FFT analysis is more sensitive to this effect. The response times show comparable behavior for both the techniques. Applying both the methods to heart rate data obtained from a neonate before and after atropine administration (inducing a wide range of HRV), shows a very significant correlation between the two methods in estimating LF and HF power. We conclude that a customized filtering technique might be beneficial for analyzing HRV in neonates because it reduces the necessary time window for signal stability.

Algorithms↗
PubMedDate not supplied

Usefulness of daily +2Gz load as a countermeasure against physiological problems during weightlessness.

UNLABELLED: Adaptation to head-down-tilt bed rest as a simulated microgravity leads to an abnormality of reflex control of circulation, hypovolemia and reduction of exercise capacity. We hypothesized that this cardiovascular deconditioning and reduction of exercise capacity could be prevented by a daily 1 hr centrifugation at +2Gz. To test this hypothesis, twenty healthy male subjects underwent 4 day of 6 degrees head-down-tilt bed rest. Ten of them were exposed to a +2Gz load for up to 30 min twice per day (the Gz group). The remaining 10 were not exposed to a Gz load (the no-Gz group). We estimated autonomic cardiovascular control by power spectral analysis of blood pressure and R-R interval variability, and baroreflex regulation by the transfer function analysis and the sequence method, before and after bed rest. Further, we measured hematocrit as an index of changes in plasma volume and maximal oxygen consumption as an index of exercise capacity, before and after bed rest. RESULT: In the no-Gz group, heart rate increased after bed rest. The high frequency power of R-R interval variability as an index of cardiac parasympathetic nervous activity, baroreflex gains estimated by transfer function analysis and the sequence method as index of the integrated arterial-cardiac baroreflex function decreased significantly. Associated with these changes, the ratio of low to high frequency power of R-R as an indicator of cardiac sympathovagal balance tended to increase after bed rest in the no-Gz group. However, those showed no significant changes after bed rest in the Gz group. Hematocrit increased after bed rest in the no-Gz group. It also tended to increase in the Gz group, however it did not achieve statistical significance. Maximal oxygen consumption decreased significantly to similar extent in both the groups. CONCLUSION: This result suggested that 1) a daily 1hr +2Gz load produced by a centrifuge might eliminate the changes in autonomic cardiovascular control during simulated weightlessness; 2) furthermore, it might partly reverse hypovolemia induced by bed rest; 3) however, it could not prevent the decreases in exercise capacity.

Baroreflex↗
PubMed2001

Relationship between apparent optical properties and photosynthetic pigments in the sub-alpine Lake Iseo.

The aim of the present study was the evaluation of methods for estimating the content of bio-physical parameters in lake water on the basis of spectral reflectance measured above water surface, in particular the estimation of chlorophyll-a (chl-a) concentrations. Data sets considered refer to some sampling point located in the sub-alpine meso-eutrophic Lake Iseo, surveyed six times over the period March-July and once in November 1998, as these months were very important for the characterization of the springtime algal bloom, which affect the lake waters yearly. At each point station, limnological observations (chlorophyll, total suspended solids, Secchi disk depth) were conducted simultaneously with optical measurements. The latter consisted of water leaving radiance measured by means of a spectroradiometer above the water surface; moreover, a standard reflector radiance was also measured to obtain the water reflectance. Reflectance spectra were transformed according to two well-documented models and correlated to water quality parameters, to investigate their performances as retrieval algorithms under different conditions and referring to different analytical methods. Results outline the sensitivity of the models to chl-a concentrations, different phytoplankton composition, and the sampling depth.

Chlorophyll↗
PubMed1997

An improved statistical methodology to estimate and analyze impedances and transfer functions.

Estimating the mathematical relationship between pulsatile time series (e.g., pressure and flow) is an effective technique for studying dynamic systems. The frequency-domain relationship between time series, often calculated as an impedance (pressure/flow), is known more generally as a frequency-response or transfer function (output/input). Current statistical methods for transfer function analysis 1) assume erroneously that repeated observations on a subject are independent, 2) have limited statistical value and power, or 3) are restricted to use in single subjects rather than in an entire sample. This paper develops a regression model for transfer function analysis that corrects each of these deficiencies. Spectral densities of the input and output time series and the cross-spectral density between them are first estimated from discrete Fourier transforms and then used to obtain regression estimates of the transfer function. Statistical comparisons of the transfer function estimates use a test statistic that is distributed as chi2. Confidence intervals for amplitude and phase can also be calculated. By correctly modeling repeated observations on each subject, this improved statistical approach to transfer function estimation and analysis permits the simultaneous analysis of data from all subjects in a sample, improves the power of the transfer function model, and has broad relevance to the study of dynamic physiological systems.

Blood Pressure↗
PubMed2000

Representation of temporal features of complex sounds by the discharge patterns of neurons in the owl's inferior colliculus.

The spiking pattern evoked in cells of the owl's inferior colliculus by repeated presentation of the same broadband noise was found to be highly reproducible and synchronized with the temporal features of the noise stimulus. The pattern remained largely unchanged when the stimulus was presented from spatial loci that evoke similar average firing rates. To better understand this patterning, we computed the pre-event stimulus ensemble (PESE)-the average of the stimuli that preceded each spike. Computing the PESE by averaging the pressure waveforms produced a noisy, featureless trace, suggesting that the patterning was not synchronized to a particular waveform in the fine structure. By contrast, computing the PESE by averaging the stimulus envelope revealed an average envelope waveform, the "PESE envelope," typically having a peak preceded by a trough. Increasing the overall stimulus level produced PESE envelopes with higher amplitudes, suggesting a decrease in the jitter of the cell's response. The effect of carrier frequency on the PESE envelope was investigated by obtaining a cell's response to broadband noise and either estimating the PESE envelope for each spectral band or by computing a spectrogram of the stimulus prior to each spike. Either method yielded the cell's PESE spectrogram, a plot of the average amplitude of each carrier-frequency component at various pre-spike times. PESE spectrograms revealed surfaces with peaks and troughs at certain frequencies and pre-spike times. These features are collectively called the spectrotemporal receptive field (STRF). The shape of the STRF showed that in many cases, the carrier frequency can affect the PESE envelope. The modulation transfer function (MTF), which describes a cell's ability to respond to time-varying amplitudes, was estimated with sinusoidally amplitude-modulated (SAM) noises. Comparison of the PESE envelope with the MTF in the time and frequency domains showed that the two were closely matched, suggesting that a cell's response to SAM stimuli is largely predictable from its response to a noise-modulated carrier. The STRF is considered to be a model of the linear component of a system's response to dynamic stimuli. Using the STRF, we estimated the degree to which we could predict a cell's response to an arbitrary broadband noise by comparing the convolution of the STRF and the envelope of the noise with the cell's post-stimulus time histogram to the same noise. The STRF explained 18-46% of the variance of a cell's response to broadband noise.

Acoustic Stimulation↗
PubMed2001

Experimental and theoretical analysis of wavelet-based denoising filter for echocardiographic images.

One of the most significant features of diagnostic echocardiographic images is to reduce speckle noise and make better image quality. In this paper we proposed a simple and effective filter design for image denoising and contrast enhancement based on multiscale wavelet denoising method. Wavelet threshold algorithms replace wavelet coefficients with small magnitude by zero and keep or shrink the other coefficients. This is basically a local procedure, since wavelet coefficients characterize the local regularity of a function. After we estimate distribution of noise within echocardiographic image, then apply to fitness Wavelet threshold algorithm. A common way of the estimating the speckle noise level in coherent imaging is to calculate the mean-to-standard-deviation ratio of the pixel intensity, often termed the Equivalent Number of Looks(ENL), over a uniform image area. Unfortunately, we found this measure not very robust mainly because of the difficulty to identify a uniform area in a real image. For this reason, we will only use here the S/MSE ratio and which corresponds to the standard SNR in case of additivie noise. We have simulated some echocardiographic images by specialized hardware for real-time application;processing of a 512*512 images takes about 1 min. Our experiments show that the optimal threshold level depends on the spectral content of the image. High spectral content tends to over-estimate the noise standard deviation estimation performed at the finest level of the DWT. As a result, a lower threshold parameter is required to get the optimal S/MSE. The standard WCS theory predicts a threshold that depends on the number of signal samples only.

Algorithms↗
PubMed1997

Estimation of axial blood velocity using the Doppler equation corrected for broadening.

To estimate axial velocity for vessels that are so narrow that their width is comparable to the minimum length of the range cell, it has been customary to substitute the maximum Doppler frequency fmax into the classical Doppler equation [eqn (1)]. It was shown here that this ignores transit time broadening, which can lead to significant errors at large beam-to-flow angles. We use a relation [eqn (2)], which does take this broadening into account, and give in vivo experimental proof of this allows accurate estimation of the axial velocity even when the range cell extends across the whole vessel lumen. It may be concluded, therefore, that, by using the above procedure for taking transit time spectral broadening into account, more accurate velocity estimates can be obtained for laminar flow than is possible with the current method using the classical Doppler equation uncorrected for broadening.

Algorithms↗
PubMed2001

Effect of prolonged head-down bed rest on complex cardiovascular dynamics.

We postulated that a change in complex dynamics of the cardiovascular system could be involved in the orthostatic intolerance observed after simulated weightlessness. Supine recordings of 1024 consecutive pulse intervals and systolic blood pressures were obtained on 7 subjects adapted to a 42 day head-down bed rest (day 22 and 42) but also before and 6 days after head-down bed rest (-6 degrees). Coarse graining spectral analysis was used to extract the non-harmonic (fractal) component from each time series. The power spectral densities of this fractal component are inversely proportional to their frequency (1/f beta). We fitted an inverse power law estimate to the fractal component to determine the spectral exponent beta. The complex dynamics of blood pressure and heart rate variability were also analyzed by correlation dimension and non-linear prediction. Bed rest induced orthostatic intolerance in 4 subjects. There was a significant increase in the spectral exponent beta of RR-interval variability during and after head-down bed rest (before: 1.039 +/- 0.090; during: 1.552 +/- 0.080 and 1.547 +/- 0.100; after: 1.428 +/- 0.040). Analysis of the blood pressure dynamics indicated lower correlation dimensions during head-down bed rest and higher coefficients of predictability after head-down bed rest. Complexity alterations of RR-interval and blood pressure variability were not linked with one another during head-down bed rest. These alterations seemed to be correlated with the orthostatic intolerance observed after bed rest. These results suggest a change of the integration level of cardiovascular autonomic regulation.

Adult↗
PubMed2001

Sympathovagal imbalance in hyperthyroidism.

We assessed sympathovagal balance in thyrotoxicosis. Fourteen patients with Graves' hyperthyroidism were studied before and after 7 days of treatment with propranolol (40 mg 3 times a day) and in the euthyroid state. Data were compared with those obtained in a group of age-, sex-, and weight-matched controls. Autonomic inputs to the heart were assessed by power spectral analysis of heart rate variability. Systemic exposure to sympathetic neurohormones was estimated on the basis of 24-h urinary catecholamine excretion. The spectral power in the high-frequency domain was considerably reduced in hyperthyroid patients, indicating diminished vagal inputs to the heart. Increased heart rate and mid-frequency/high-frequency power ratio in the presence of reduced total spectral power and increased urinary catecholamine excretion strongly suggest enhanced sympathetic inputs in thyrotoxicosis. All abnormal features of autonomic balance were completely restored to normal in the euthyroid state. beta-Adrenoceptor antagonism reduced heart rate in hyperthyroid patients but did not significantly affect heart rate variability or catecholamine excretion. This is in keeping with the concept of a joint disruption of sympathetic and vagal inputs to the heart underlying changes in heart rate variability. Thus thyrotoxicosis is characterized by profound sympathovagal imbalance, brought about by increased sympathetic activity in the presence of diminished vagal tone.

Adult↗
PubMed2006

Nonlinear analysis of blood cell flux fluctuations in the rat brain cortex during stepwise hypotension challenge.

Blood flow through a region of interest in the brain cortex (cerebral blood flow (CBF)) as measured by laser-Doppler flowmetry (LDF) shows a complex temporal pattern, which can be either merely random or a manifestation of segmental chaotic dynamics of vasomotion-induced flowmotion in the arterial tree, a deterministic phenomenon; or that of a fractal, self-similar correlation order that emerges from the set of segmental perfusion events on statistical ground. Fractal content (F%) was determined by coarse-graining spectral analysis and their self-similar exponent, H, estimated by bridge-detrended Scaled Windowed Variance (bdSWV) method and a variant of the power Spectral Density method ((low)PSD(w,e)). Chaotic dynamics were assessed by computing the correlation dimension (D(corr)) and the largest Lyapunov exponent (lambda(max)) on unfiltered raw and surrogate datasets. In 10 Sprague-Dawley rats anesthetized by halothane, CBF was measured through the thinned calvarium by the LDF method. Blood pressure (BP) was reduced from 100 to 40 mm Hg in steps of 20 mm Hg maintained for 2 mins by the lower body negative pressure method. Fractal and chaotic patterns coexisted in tissue perfusion. CBF did not show autoregulation. At every BP step, F% remained high (72% to 88%) and was independent of BP. Laser-Doppler flowmetry signals proved to be nonstationary fractional Brownian motions. Their H's by bdSWV and (low)PSD(w,e) (0.29+/-0.006 and 0.25+/-0.012, respectively) were independent of BP. Neither D(corr) nor lambda(max) varied with hypotension. Their values were characteristic of a chaotic system, but surrogate data analysis rendered some of them inconclusive. Hence, CBF fluctuations can be regarded as a robust phenomenon that is not abolished even by sustained hypotension at 40 mm Hg.

Animals↗
PubMed2000

Turboprop and rotary-wing aircraft flight parameter estimation using both narrow-band and broadband passive acoustic signal-processing methods.

Flight parameter estimation methods for an airborne acoustic source can be divided into two categories, depending on whether the narrow-band lines or the broadband component of the received signal spectrum is processed to estimate the flight parameters. This paper provides a common framework for the formulation and test of two flight parameter estimation methods: one narrow band, the other broadband. The performances of the two methods are evaluated by applying them to the same acoustic data set, which is recorded by a planar array of passive acoustic sensors during multiple transits of a turboprop fixed-wing aircraft and two types of rotary-wing aircraft. The narrow-band method, which is based on a kinematic model that assumes the source travels in a straight line at constant speed and altitude, requires time-frequency analysis of the acoustic signal received by a single sensor during each aircraft transit. The broadband method is based on the same kinematic model, but requires observing the temporal variation of the differential time of arrival of the acoustic signal at each pair of sensors that comprises the planar array. Generalized cross correlation of each pair of sensor outputs using a cross-spectral phase transform prefilter provides instantaneous estimates of the differential times of arrival of the signal as the acoustic wavefront traverses the array.

Acoustics↗
PubMed1984

Leakage of Dhurrin and p-Hydroxybenzaldehyde from Young Sorghum Shoots Immersed in Various Solvents.

Spectral scanning was used to provide estimates of the leakage of the cyanogenic glucoside, dhurrin (p-hydroxy-[S]-mandelonitrile-beta-d-glucoside), and its metabolite, p-hydroxybenzaldehyde (p-HB), from young light-grown shoots of Atlas sorghum (Sorghum bicolor [L.] Moench) when these shoots were immersed in water, toluene, chloroform or mixtures of water and toluene or water and chloroform. Minimal leakage of dhurrin and virtually no leakage of p-HB occurred with water as the solvent. The 0.5% concentration (v/v) of both toluene and chloroform was more effective than either the 1.0 or 2.0% concentrations in effecting leakage of the two solutes. With either 0.5% toluene or 0.5% chloroform as the solvent, 80 to 90% of the total dhurrin was extracted from shoots in a 3-hour period. Breakdown of dhurrin during extraction was much more extensive with 0.5% chloroform than with 0.5% toluene. Some loss of p-HB occurred during 3- or 6-hour extractions in the water-organic solvent mixtures; spectral and chromatographic evidence suggested partial conversion of p-HB to p-hydroxybenzoic acid. With undiluted toluene or chloroform as solvents, extracts contained appreciable amounts of free p-HB but essentially no dhurrin. These solvents were less effective than the water-organic solvent mixtures in extracting the solutes from the shoot issue.

Journal Article↗
PubMed2005

Comparison of label-free methods for quantifying human proteins by shotgun proteomics.

Measurements of mass spectral peak intensities and spectral counts are promising methods for quantifying protein abundance changes in shotgun proteomic analyses. We describe Serac, software developed to evaluate the ability of each method to quantify relative changes in protein abundance. Dynamic range and linearity using a three-dimensional ion trap were tested using standard proteins spiked into a complex sample. Linearity and good agreement between observed versus expected protein ratios were obtained after normalization and background subtraction of peak area intensity measurements and correction of spectral counts to eliminate discontinuity in ratio estimates. Peak intensity values useful for protein quantitation ranged from 10(7) to 10(11) counts with no obvious saturation effect, and proteins in replicate samples showed variations of less than 2-fold within the 95% range (+/-2sigma) when >or=3 peptides/protein were shared between samples. Protein ratios were determined with high confidence from spectral counts when maximum spectral counts were >or=4 spectra/protein, and replicates showed equivalent measurements well within 95% confidence limits. In further tests, complex samples were separated by gel exclusion chromatography, quantifying changes in protein abundance between different fractions. Linear behavior of peak area intensity measurements was obtained for peptides from proteins in different fractions. Protein ratios determined by spectral counting agreed well with those determined from peak area intensity measurements, and both agreed with independent measurements based on gel staining intensities. Overall spectral counting proved to be a more sensitive method for detecting proteins that undergo changes in abundance, whereas peak area intensity measurements yielded more accurate estimates of protein ratios. Finally these methods were used to analyze differential changes in protein expression in human erythroleukemia K562 cells stimulated under conditions that promote cell differentiation by mitogen-activated protein kinase pathway activation. Protein changes identified with p<0.1 showed good correlations with parallel measurements of changes in mRNA expression.

Bias↗
PubMed1998

Repeatability of electrically evoked EMG signals in the human vastus medialis muscle.

The repeatability of spectral and amplitude parameters and conduction velocity estimates of M-waves was tested on the vastus medialis muscle of 9 normal subjects. Isometric contractions sustained for 30 s were elicited by stimulation of the main muscle motor point and repeated on 5 different days. The initial value and two indicators of myoelectric manifestations of muscle fatigue were computed for each variable. The test-retest Pearson correlation coefficient, the paired Wilcoxon test, and the intraclass correlation coefficient (ICC) showed that parameters of spectral variables are more repeatable than those of amplitude variables and conduction velocity. The ICC ranged from 86.4% for the initial value of median frequency to 21.6% for the initial value of the average rectified value. Fatigue indices showed ICCs in the range of 20-64%. It is concluded that: (a) initial values and fatigue indices based on spectral variables are more repeatable than those based on amplitude variables; (b) the repeatability of conduction velocity estimates is not yet sufficient for clinical application; (c) M-wave shape, rather than amplitude or width, is a characteristic of individual muscles; and (d) electrode location is critical.

Adult↗