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[Effect of hydroxylamine hydrochloride on lipopolysaccharide fatty acid composition in Shigella dysenteriae 1].

Changes in the fatty acid composition of S. dysenteriae 1 lipid A after the treatment of lipopolysaccharide (LPS) with hydrosylamine hydrochloride (HH) and 4 degrees C, 37 degrees C and 56 degrees C were studied with the use of gas-liquid chromatographicmass-spectrometry. The treatment with HH led to a decrease in the toxicity of LPS, but produced no changes in the content of the main fatty acid components of lipid A (lauric, myristic, oxymyristic and palmitic acids). At the same time the total number of minor fatty acid derivatives decreased from 11 (in the original LPS) to 5 in LPS treated with HH at 56 degrees C.

Antigens, Bacterial↗
PubMed1975

Mechanism of the mutagenic action of hydroxylamine. VIII. Functional properties of the modified adenosine residues.

The action of O-methylhydroxylamine in vitro upon some amber and rII ochre mutants of T4 phage leads to a considerable increase in the frequency of reversions and conversions of ochre and opal mutants presumably due to A yields G transitions. The transitions seem to be caused by modification of the adenine bases of the genome to give N-6-methoxyadenine which has equivocal (A- and G-like) specificity. However, the functional activity of N-6-methoxyadenosine residues in the RNA polymerase system is low. We failed to detect any significant activity of these residues either as components of a template polyribonucleotide, or as nucleoside triphosphate precursors.

Adenine Nucleotides↗