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PubMed2006

Seroprevalence study of feline coronavirus in owned and feral cats in Sydney, Australia.

OBJECTIVES: i) To establish the seroprevalence of Feline Coronavirus (FCoV) infection in two defined groups of cats in Sydney: owned and feral cats; ii) to identify factors associated with an increased risk of infection with FCoV; and iii) to establish the seroprevalence and FCoV antibody titres of owned cats with immunohistochemically confirmed feline infectious peritonitis (FIP). DESIGN: Prospective multi-institutional cross sectional study. Procedure Serum samples from owned cats presented to three inner city veterinary clinics in Sydney and feral cats from a colony in South Western Sydney over an 11-month period were tested for FCoV antibodies using the Immunocomb test kit. The relationship between serological score and six major factors (breed, age, gender, number of cats per household, living environment and health status) in the owned cat sample population was analysed and compared to cats with FIR RESULTS: The seroprevalence of FCoV infection in the sample population of owned and feral cats was 34% and 0%, respectively. The median Immunocomb scores of DSH, Persian, Siamese and Devon Rex cats were significantly lower than that of Burmese, BSH, Abyssinian, Birman, Ragdoll and Russian Blue. The median lmmunocomb score of pedigree cats less than 2 years-of-age was significantly higher than for pedigree cats greater than 2 years-of-age. This distinction was not evident in DSH cats in these age groups. The number of cats per household at the time of blood collection had a strong positive association with Immunocomb score. The median Immunocomb score of cats with immunohistochemically confirmed FIP was significantly higher than cats in the sample population of owned cats but there was sufficient overlap between these two groups to make definitive diagnosis of FIP by serology impossible. CONCLUSION: This represents the first seroprevalence study of FCoV in Australia. The major determinants of antibody score of owned cats identified in this study were breed, age and the number of cats per household. The significant relationship between the breed of the cat and the FCoV antibody titre further supports the notion, proposed previously by the authors, that breed related differences exist in the immunological response to FCoV infection.

Age Factors↗
PubMedDate not supplied

The relationship between the feline coronavirus antibody titre and the age, breed, gender and health status of Australian cats.

OBJECTIVES: To investigate the relationship between Feline Coronavirus (FCoV) antibody titres and age, breed, gender and health status of Australian cats DESIGN: Retrospective study PROCEDURE: Results from two serological tests that measure FCoV antibody levels, the Coronase test and the 7B Feline Infectious Peritonitis (FIP) test, were recorded over a 2-year period, with patient signalment, history, presenting complaint and the reason for ordering the test (as available). Results from each antibody test were related to four explanatory variables (breed, age, gender and health status at the time of blood collection) using univariate ordinal logistic regression analyses, Mann Whitney U tests, one-sample sign tests or Kruskal-Wallis analyses, as appropriate. RESULTS: Results from 637 Coronase and 191 7B FIP antibody tests were recorded. There were significant differences in median Coronase antibody titres between breeds of cats (P < 0.0005). Specifically, the median Coronase antibody titres of Siamese, Persians, Domestic Shorthairs and Bengal cats (100) were significantly lower than that of British Shorthairs, Cornish Rex and Burmese cats (400, P < 0.0005). There was no statistical relationship between the Coronase or 7B FIP antibody titres and age, gender or overall health status, even when considering only those cats in which clinical signs suggestive of FIP were present. CONCLUSION: This study reinforces the complexity of interpreting serological tests for FCoV in both healthy cats and patients with signs compatible with FIR Unique to this study is the detection of a significant relationship between breed and median FCoV antibody titre. This supports the theory that breed related differences exist in response to FCoV infection. The distribution of median Coronase antibody titres by breed was very similar to the pattern of breed predisposition to FIP recently reported in Sydney.

Age Factors↗
PubMed2001

Modeling of the deformation of flexible tubes using a single law: application to veins of the lower limb in man.

The topic of this study mainly concerns a representative model of the behavior of flexible ducts such as elastic tubes or veins. This model is based on a phenomenological approach of the inflation and collapse of the tube. It leads to a single "universal" analytical expression of the tube law, valid fir a wide range of' positive and negative transmural pressures, which presents a significant improvement compared to previous theoretical studies defined with different expressions on restricted ranges of pressure. Moreover, the theoretical approaches most often require simplif'ing hypotheses--no longitudinal tension, no surrounding tissues--which are quite unrealistic both in the physiological case and in the experimental setup. These theoretical models can therefore be expected only roughly to describe the actual behavior of such vessels. The representative model, on the contrary, allows one to account for the deformation--inflating as well as collapse--of elastic tubes or veins with better accuracy. The tube law is a function of six parameters chosen in order to fit the experimental data. A comparison between results obtained in our laboratory using silicone tubes and representative models is presented. The model is then applied to physiological data obtained in vivo on human leg veins.

Elasticity↗
PubMed2001

Room reverberation effects in hearing aid feedback cancellation.

Room reverberation can affect feedback cancellation in hearing aids, with the strength of the effects depending on the acoustical conditions. These effects were studied using a behind the ear (BTE) hearing aid mounted on a dummy head and coupled to the ear canal via an open fitting. The hearing aid impulse response was measured for the dummy head placed at eight closely spaced locations in a typical office. The feedback cancellation in the hearing aid used a set of filter coefficients that were initialized for one location within the room, and then allowed to adapt to the feedback path measured at the same or to a different location. The maximum stable gain for the hearing aid was then estimated without feedback cancellation, for the initial set of feedback cancellation filter coefficients prior to adaptation, and for the feedback cancellation filter after adaptation. A low-order ARMA model combining a fixed set of poles with an adaptive FIR filter is shown to be effective in representing the feedback path exclusive of reverberation. Increasing the adaptive filter length has only a small benefit in improving the feedback cancellation performance due to the inability of the system to model the room reverberation. The mismatch between the modeled and actual feedback paths limits the headroom increase that can be achieved when using feedback cancellation, and varies with the location within the room.

Acoustics↗
PubMed2001

Spatially extended sound equalization in rectangular rooms.

The results of a theoretical study on global sound equalization in rectangular rooms at low frequencies are presented. The zone where sound equalization can be obtained is a continuous three-dimensional region that occupies almost the complete volume of the room. It is proved that the equalization of broadband signals can be achieved by the simulation of a traveling plane wave using FIR filters. The optimal solution has been calculated following the traditional least-squares approximation, where a modeling delay has been applied to minimize reverberation. An advantage of the method is that the sound field can be estimated with sensors placed in the limits of the equalization zone. As a consequence, a free space for the listeners can be obtained.

Journal Article↗
PubMed2003

Derivation of an optimal directivity pattern for sweet spot widening in stereo sound reproduction.

In this paper the correction of the degradation of the stereophonic illusion during sound reproduction due to off-center listening is investigated. The main idea is that the directivity pattern of a loudspeaker array should have a well-defined shape such that a good stereo reproduction is achieved in a large listening area. Therefore, a mathematical description to derive an optimal directivity pattern l(opt) that achieves sweet spot widening in a large listening area for stereophonic sound applications is described. This optimal directivity pattern is based on parametrized time/intensity trading data coming from psycho-acoustic experiments within a wide listening area. After the study, the required digital FIR filters are determined by means of a least-squares optimization method for a given stereo base setup (two pair of drivers for the loudspeaker arrays and 2.5-m distance between loudspeakers), which radiate sound in a broad range of listening positions in accordance with the derived l(opt). Informal listening tests have shown that the l(opt) worked as predicted by the theoretical simulations. They also demonstrated the correct central sound localization for speech and music for a number of listening positions. This application is referred to as "Position-Independent (PI) stereo."

Acoustics↗
PubMed2003

Lattice form adaptive infinite impulse response filtering algorithm for active noise control.

In some situations of active noise control, infinite impulse response (IIR) filters are more suitable than finite impulse response (FIR) filters owing to the poles in the transfer function. A number of algorithms have been derived for applying IIR filters in active noise control; however, most of them use the direct form IIR filter structure, which faces the difficulties of checking stability and relatively slow convergence speed for noise composed of narrow-band components with large power disparity. To overcome these difficulties along with using the direct form IIR filters, a new adaptive algorithm is proposed in this paper, which uses and updates the lattice form adaptive IIR filter in an active noise control system. Full mathematical derivations of the proposed algorithm are presented, and the comparison between the proposed algorithm and the commonly used filtered-u LMS and filtered-v LMS algorithms shows the superiority of the proposed algorithm.

Journal Article↗
PubMed2004

Intelligibility of bandpass filtered speech: steepness of slopes required to eliminate transition band contributions.

Despite the recognition that the steepness of filter slopes can play an important role in the intelligibility of bandpass speech, there has been no systematic examination of its importance. The present study used high orders of finite impulse response (FIR) filtering to produce slopes ranging from 150 to 10,000 dB/octave. The slopes flanked 1/3-octave passbands of everyday sentences having a center frequency of 1500 Hz (the region of highest intelligibility for the male speaker's voice). Presentation levels were approximately 75 and 45 dB. No significant differences were found for the two presentation levels. Average intelligibility scores ranged from 77% at 150 dB/octave down to the asymptotic intelligibility score of 12% at 4800 dB/octave. These results indicate that slopes of several thousand dB/octave may be required for accurate and unambiguous specification of the range of frequencies contributing to intelligibility of filtered speech. In addition, the extremely steep slopes are needed to ensure that none of the spectral components contributing to intelligibility has its relative importance diminished by spectral tilt.

Acoustics↗
PubMed2006

Model-based sound synthesis of the guqin.

This paper presents a model-based sound synthesis algorithm for the Chinese plucked string instrument called the guqin. The instrument is fretless, which enables smooth pitch glides from one note to another. A version of the digital waveguide synthesis approach is used, where the string length is time-varying and its energy is scaled properly. A body model filter is placed in cascade with the string model. Flageolet tones are synthesized with the so-called ripple filter structure, which is an FIR comb filter in the delay line of a digital waveguide model. In addition, signal analysis of recorded guqin tones is presented. Friction noise produced by gliding the finger across the soundboard has a harmonic structure and is proportional to the gliding speed. For pressed tones, one end of a vibrating string is terminated either by the nail of the thumb or a fingertip. The tones terminated with a fingertip decay faster than those terminated with a thumb. Guqin tones are slightly inharmonic and they exhibit phantom partials. The synthesis model takes into account these characteristic features of the instrument and is able to reproduce them. The synthesis model will be used for rule based synthesis of guqin music.

Humans↗
PubMed1999

Intelligibility of 1/3-octave speech: greater contribution of frequencies outside than inside the nominal passband.

We reported previously that "everyday" sentences were highly intelligible when limited to a 1/3-octave passband centered at 1,500 Hz and having transition-band slopes of approximately 100 dB/octave. The present study determined the relative contributions to intelligibility made by the passband (PB) and the transition bands (TBs) by partitioning the same bandpass sentences using 2,000-order FIR filtering. Intelligibility scores were: PB with both TBs, 92%; deletion of both TBs (leaving only the 1/3-octave PB with nearly vertical slopes), 24%; deletion of the PB (leaving both TBs separated by a 1/3-octave gap), 83%. These and other results indicate a remarkable ability to compensate for severe spectral tilt and the consequent importance of considering frequencies outside the nominal passband in interpreting studies using filtered speech.

Humans↗
PubMed2000

A relaxed condition for "perfect" cancellation of broadband noise in 3D enclosures

This paper presents a relaxed condition for "perfect" cancellation of broadband noise in 3D enclosures. On the basis of a truncated modal model, it can be shown that the primary and secondary paths belong to a same subspace if a certain condition is satisfied. There exists a finite impulse response (FIR) filter transfer function vector for perfect cancellation of the primary paths. The analytical result is verified numerically with an active noise control (ANC) system in a 3D rectangular enclosure. The proposed ANC scheme is shown to fit well into the framework of an existing multichannel least-mean squares (LMS) algorithm for adaptive implementation.

Journal Article↗
PubMed1981

Microbiology of wetwood: importance of pectin degradation and clostridium species in living trees.

Wetwood samples from standing trees of eastern cottonwood (Populus deltoides), black poplar (Populus nigra), and American elm (Ulmus americana) contained high numbers of aerobic and anaerobic pectin-degrading bacteria (10 to 10 cells per g of wood). High activity of polygalacturonate lyase (</=0.5 U/ml) was also detected in the fetid liquid that spurted from wetwood zones in the lower trunk when the trees were bored. A prevalent pectin-degrading obligately anaerobic bacterium isolated from these wetwoods was identified as Clostridium butyricum. Pectin decomposition by C. butyricum strain 4P1 was associated with an inducible polygalacturonate lyase and pectin methylesterase, the same types of pectinolytic activity expressed in the wetwood of these trees. The pH optimum of the extracellular polygalacturonate lyase was alkaline (near pH 8.5). In vitro tests with sapwood samples from a conifer (Douglas fir, Pseudotsuga menziesii) showed that tori in membranes of bordered pits are degraded by pure cultures of strain 4P1, polygalacturonate lyase enzyme preparations of strain 4P1, and mixed methanogenic cultures from the tree samples of wetwood. These results provide evidence that pectin in xylem tissue is actively degraded by C. butyricum strain 4P1 via polygalacturonate lyase activity. The importance of pectin degradation by bacteria, including Clostridium species, appears paramount in the formation and maintenance of the wetwood syndrome in certain living trees.

Journal Article↗
PubMed1985

Cellulase and Other Polymer-Hydrolyzing Activities of Trichomitopsis termopsidis, a Symbiotic Protozoan from Termites.

Crude extracts of the anaerobic, cellulolytic protozoan Trichomitopsis termopsidis possessed endo-beta-1,4-glucanase and cellobiase activities, as evidenced by hydrolytic action on carboxymethyl cellulose and cellobiose, respectively. Cell extracts also hydrolyzed microcrystalline cellulose. Hydrolysis of microcrystalline cellulose displayed optima at pH 5 and at 30 degrees C, and glucose was the sole product liberated. Cellulolytic activities of T. termopsidis appeared to be entirely cell associated. Hydrolytic activity was also detected against Douglas fir wood powder, xylan, starch, and protein, but not chitin. The importance of these enzymes in the nutrition of T. termopsidis is discussed in terms of the natural habitat of this protozoan (the hindgut of wood-eating termites).

Journal Article↗
PubMed1985

Nitrogen dynamics in stream wood samples incubated with [C]lignocellulose and potassium [N]nitrate.

Surface wood samples obtained from a Douglas fir log (Pseudotsuga menziesii) in a Pacific Northwest stream were incubated in vitro with [C]lignocellulose in a defined mineral salts medium supplemented with 10 mg of N liter of N-labeled NO(3) (50 atom% N). Evolution of CO(2), distribution and isotopic dilution of N, filtrate N concentrations, and the rates of denitrification, N(2) fixation, and respiration were measured at 6, 12, and 18 days of incubation. The organic N content of the lignocellulose-wood sample mixture had increased from 132 mug of N to a maximum of 231 mug of N per treatment after 6 days of incubation. Rates of [C]lignocellulose decomposition were greatest during the first 6 days and then began to decline over the remaining 12 days. Total CO(2) evolution was also highest at day 6 and declined steadily over the remaining duration of the incubation. Filtrate NH(4)-N increased from background levels to a final value of 57 mug of N per treatment. Filtrate NO(3) N completely disappeared by day 6, and organic N showed a slight decline between days 12 and 18. The majority of the N that could be recovered appeared in the particulate organic fraction by day 6 (41 mug of N), and the filtrate NH(4) N fraction contained 11 mug of N by day 18. The N enrichment values of the filtrate NH(4) and the inorganic N associated with the particulate fraction had increased to approximately 20 atom% N by 18 days of incubation, whereas the particulate organic fraction reached its highest enrichment by day 6. Measurements of N(2) fixation and denitrification indicated an insignificant gain or loss of N from the experimental system by these processes. The data show that woody debris in stream ecosystems might function as a rapid and efficient sink for exogenous N, resulting in stimulation of wood decomposition and subsequent activation of other N cycling processes.

Journal Article↗
PubMed1986

Effects of pH on Lignin and Cellulose Degradation by Streptomyces viridosporus.

Lignocellulose degradation by Streptomyces viridosporus results in the oxidative depolymerization of lignin and the production of a water-soluble lignin polymer, acid-precipitable polymeric lignin (APPL). The effects of the culture pH on lignin and cellulose metabolism and APPL production by S. viridosporus are reported. Dry, ground, hot-water-extracted corn (Zea mays) lignocellulose was autoclaved in 1-liter reagent bottles (5 g per bottle) and inoculated with 50-ml volumes of S. viridosporus cells suspended in buffers of specific pH (pH 6.0 to 9.2 at 0.4 pH unit intervals). Four replicates of inoculated cultures and of uninoculated controls at each pH were incubated as solid-state fermentations at 37 degrees C. After 6 weeks of incubation the percent loss of lignocellulose, lignin, and carbohydrate and the amount of APPL produced were determined for each replicate. Optimal lignocellulose degradation, as shown by substrate weight loss, was observed in the pH range of 8.4 to 8.8. Only minor differences were seen in the Klason lignin, carbohydrate, protein, and ash contents of the APPLS produced by cultures at each pH. The effects of pH on the degradation of a spruce (Picea pungens) [C-lignin]lignocellulose and a Douglas fir (Pseudotsuga menziesii) [C-glucan]-lignocellulose were also determined at pH values between 6.5 and 9.5 (0.5 pH unit intervals). The incubations were carried out for 3 weeks at 37 degrees C with bubbler-tube cultures. The percentage of initial C recovered as CO(2), C-labeled water-soluble products, and [C]APPL was then determined. The mineralization of lignin and cellulose to CO(2) was optimal at pHs 6.5 and 7.0, respectively. However, the optimum for lignin and cellulose solubilization was pH 8.5, which correlated with the pH 8.5 optimum for APPL production. Overall, the data show that, whereas lignin mineralization is optimal at neutral to slightly acidic pHs, lignocellulose degradation with lignin solubilization and APPL production is promoted by alkaline pHs. These findings indicate that lignin-solubilizing actinomycetes may play an important role in the metabolism of lignin in neutral to alkaline soils in which ligninolytic fungi are not highly competitive.

Journal Article↗
PubMed1987

Biosynthesis of Indole-3-Acetic Acid by the Pine Ectomycorrhizal Fungus Pisolithus tinctorius.

Previous work has indicated that anatomical and morphological changes (stunting and dichotomy) in roots of various conifers may be influenced by plant-growth-regulating substances secreted by mycorrhizae. Indole-3-acetic acid (IAA) has been tentatively identified as a major auxin produced by some selected ectomycorrhizae. We report the isolation and detection of IAA as a secondary metabolite from Pisolithus tinctorius by thin-layer chromatography, high-performance liquid chromatography (HPLC), enzyme-linked immunosorbent (monoclonal antibody) assay (ELISA), and unequivocal identification by gas chromatography-mass spectrometry (GC-MS). The thin-layer chromatography methods for auxin isolation described here are novel, with the use of heptane-acetone-glacial acetic acid as the migrating solvent and formaldehyde, H(2)SO(4), and vanadate in detection. The acidic extract of the culture supernatant was methylated with ethereal diazomethane to detect IAA as methyl-3-IAA by HPLC, ELISA, and GC-MS. The quantitative amount of IAA detected ranged from 4 to 5 mumol liter by HPLC and ELISA. Another unidentified metabolite was detected by GC-MS with a typical indole nucleus (m/z = 130), indicating that it could be an intermediate in auxin metabolism. Plant response (Pseudotsuga menziesii, Douglas fir) was monitored upon inoculation of P. tinctorius and l-tryptophan. There was a consistent increase in plant height and stem diameter as a result of the two treatments, with statistical differences in dry weights of the shoots and roots.

Journal Article↗
PubMed1997

Cellobiose dehydrogenase, an active agent in cellulose depolymerization.

The ability of cellobiose dehydrogenase purified from Phanerochaete chrysosporium to modify a Douglas fir kraft pulp was assessed. Although the addition of cellobiose dehydrogenase alone had little effect, supplementation with cellobiose and iron resulted in a substantial reduction in the degree of polymerization of the pulp cellulose. When the reaction was monitored over time, a progressive depolymerization of the cellulose was apparent with the concomitant production of cellobiono-1,5-lactone. Analysis of the reaction filtrates indicated that glucose and arabinose were the only neutral sugars generated. These sugars are derived from the degradation of the cellobiose rather than resulting from modifications of the pulp. These results suggest that the action of cellobiose dehydrogenase results in the generation of hydroxyl radicals via Fenton's chemistry which subsequently results in the depolymerization of cellulose. This appears to be the mechanism whereby a substantial reduction in the degree of polymerization of the cellulose can be achieved without a significant release of sugar.

Journal Article↗