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At least 829 records · Page 46Linked to original sources
PubMed2006

[RP-HPLC fingerprint for quality assessment of Radix Angelicae dahuricae].

AIM: To establish the RP-HPLC fingerprint analysis for the quality control of Radix Angelicae dahuricae. METHODS: HPLC fingerprint analysis method of Radix Angelicae dahuricae was developed. Kromasil C18 column (250 mm x 4.6 mm ID, 5 microm) was used, with mixture of acetonitrile and water as mobile phase in a gradient mode. The flow rate was 1.0 mL x min(-1). The wavelength of measurement was 254 nm. Twenty-one batches of Radix Angelicae dahuricae were determined. RESULTS: The 21 samples were classified as 4 clusters by cluster analysis and the 11 superior in producing area samples were confirmed to establish the mutual model. The samples' quality was assessed by Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004. CONCLUSION: The method can be used to identify and evaluate the quality of Radix Angelicae dahuricae conveniently.

Angelica↗
PubMed2006

[Fingerprints of Rhizoma Coptidis from Shizhu by HPLC].

OBJECTIVE: To establish a sensitive and specific HPLC method for the quality control of Rhizoma Coptidis collected from shizhu in chongqing. METHODS: The HPLC fingerprints of Rhizoma Coptidis from shizhu were obtained from Waters instrument. The methods was performed on a Diamonsil C18 column gradient eluted with acetonitrile-0.05 mol/L KH2PO4 (pH3 with H3PO4) at the flow rate of 0.8 m/min. The temperature of column was 25 degrees C and the UV detection wavelength was 270nm. RESULTS: The HPLC fingerprint of Rhizoma Coptis, showing 16 characteristic peaks, was established from 10 lots of Rhizoma Coptis. By comparision of the retention time and the on-line UV spectra of chemical standards, peak 11, 12, 13, 14 and 15 were identified as Epiberberine, Jatrorrhizine, Coptisine, Palmatine and Berberine. CONCLUSION: The HPLC fingerprint of Rhizoma Coptidis with high specificity can be used to control its quality.

Berberine↗
PubMed2006

[Establishment of RP-HPLC fingerprint for quality assessment of Cortex Periplocae].

OBJECTIVE: To establish the RP-HPLC fingerprint analysis for the quality control of Cortex Periplocae. METHODS: HPLC fingerprint and analysis method of Cortex Periplocae were studied. Kromasil C18 column (250 mm x 4.6 mm, 5 microm) was used, with mixture of acetonitrile and 0.025% phosphate solution as mobile phase in a gradient mode. The flow rate was 1.0 ml/min. The wavelength of measurement was 220 nm. 15 Batches of Cortex Periplocae were determined. RESULTS: The 15 samples were classified as 4 clusters by cluster analysis and the 6 superior in producing area samples were confirmed to establish the mutual model. The samples' quality was assessed by "Similarity Evaluation System for Chromatographic Fingerprint of TCM 2004". CONCLUSION: The method is simple and reliable. It can be used to evaluate and control the quality of Cortex Periplocae conveniently.

Chromatography, High Pressure Liquid↗
PubMed2006

[Qualitative fingerprint and quantitative determination of caffeic acid in compound dandelion enema].

OBJECTIVE: To establish a qualitative and quantitative reversed-phase high-performance liquid chromatography (RP-HPLC) with fingerprinting technique for quality control of compound dandelion enema. METHODS: HPLC was utilized for quality assessment of 10 batches of samples. RP-HPLC analysis was performed on a Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm) with the mixture of acetonitrile (A) and potassium phosphate solution (B) (pH3.2) as the mobile phase in gradient mode. The concentrations of solvent A were 10%, 80% and 80% at 0, 38 and 40 min, respectively. The column temperature was set at 35 degrees C, the flow rate at 0.7 ml/min and the detection wavelength at 254 nm. RESULTS: HPLC fingerprinting was established from the 10 batches, and the data showed 23 characteristic peaks in the compound dandelion enema for use as index peaks for qualitative identification. Comparison of the retention time and the on-line UV spectra of the samples with the chemical standards identified peaks 3, 4 and 8 as protocatechualdehyde, caffeic acid and ferulic acid, respectively. The contents of caffeic acid in the compound dandelion enema ranged between 63.7 and 136.8 microg/ml. CONCLUSION: High specific chromatographic fingerprinting and quantitative measurement of caffeic acid allows rigorous quality control of compound dandelion enema.

Caffeic Acids↗
PubMed2006

[Application of HPLC fingerprint in quality control of Fufangteng combination formula].

OBJECTIVE: To establish the characteristic mode of HPLC fingerprint of Fufangteng combination formula for the quality control of this medicine. METHOD: 10 batch certified products were selected to establish the characteristic mode of HPLC fingerprint of Fufangteng combination formula by chromatographic pattern matching and retention time correction. RESULT: This characteristic mode of HPLC fingerprint can clearly differentiate the eligible Fufangteng products from the unqualified products. CONCLUSION: This method can provide thorough information for the quality control of Fufangteng combination formula.

Administration, Oral↗
PubMed1976

Studies on the poky mutant of eurospora crassa. Fingerprint analysis of mitochondrial ribosomal RNA.

Base sequence and methylation of mitochondrial ribosomal RNAs from wild type and poky strains of Neurospora crassa were compared to determine whether a mutational lesion exists in poky 19 S RNA. At the outset, new procedures were developed for the isolation of intact nucleic acids from Neurospora mitochondria based on the substitution of Ca2+ for Mg2+ in the isolation media to inhibit mitochondrial nuclease activity. Using these procedures, intact and highly purified 32P-labeled ribosomal RNAs were extracted from purified mitochondrial ribosomal subunits of wild type and poky and compared using three complementary fingerprinting systems: two-dimensional electrophoresis of T1 plus phosphatase digests and homochromatography of T1 and pancreatic RNase digests. In supplementary experiments, 32P-labeled wild type RNA was co-fingerprinted with 32P-labeled poky and ratios of 32P/33P radioactivity were determined in each fragment to detect possible differences in stoichiometry. In addition, levels and patterns of methylated nucleotides were compared using procedures based on in vivo labeling with [methyl-3H]methionine and [32P]orthophosphate. In all these experiments, no difference was detected between wild type and poky in base sequence or methylation of either 19 S or 25 S RNA. Levels of methylation of Neurospora mitochondrial ribosomal RNAs were extremely low (less than 0.1% of the nucleotides), and results based on fingerprint analysis and DEAE-cellulose chromatography of alkaline hydrolysates of the [3H]methyl-labeled RNA suggested that 25 S RNA contains two ribose methylations, while 19 S RNA contains no methylated nucleotides.

Calcium↗
PubMed1991

[Taylorella equigenitalis: cell wall proteins, gene fingerprints, plasmids, adhesion and toxicity].

In this study 55 strains of Taylorella equigenitalis isolated from horses of four different studs in Austria, and a comparative strain from the Federal Republic of Germany were investigated by different methods. These investigations were carried out with the help of SDS-PAGE, immunoblotting, the analyses of genomes and by proof of plasmids. Furthermore, pathogenic mechanisms such as adhesion or the formation of toxins were investigated in vitro. On the basis of the results carried out by means of SDS-PAGE and immunoblotting all tested strains of Taylorella equigenitalis were alike, whereas by DNA analyses the strains could be divided into five groups. The comparative strain from the FRG, which clearly differed from the Austrian strains, formed one group all by itself. From three studs, which are related to each other because of an intensive exchange of horses, representatives (n = 53) of three DNA fingerprint groups were isolated. These three fingerprint patterns were very similar to each other, while the hybridisation patterns from the other two Austrian strains were very different. One of these strains, isolated from a diseased mare, could not be distinguished from the other strain isolated from a clinical healthy stallion from the same study by this method. Only 47.3% from the investigated strains showed attachment to HeLa cells, while cell extracts of all of them caused morphological changes of a varying degree of both Y1 and Vero cells. There were no connexions between these adhesion-cytotoxicity-properties and the DNA fingerprint groups as well as the studs, respectively. No plasmids were found in the Taylorella equigenitalis strains used in this study.

Animals↗
PubMed1990

DNA fingerprinting for forensic identification: potential effects on data interpretation of subpopulation heterogeneity and band number variability.

Some methods of statistical analysis of data on DNA fingerprinting suffer serious weaknesses. Unlinked Mendelizing loci that are at linkage equilibrium in subpopulations may be statistically associated, not statistically independent, in the population as a whole if there is heterogeneity in gene frequencies between subpopulations. In the populations where DNA fingerprinting is used for forensic applications, the assumption that DNA fragments occur statistically independently for different probes, different loci, or different fragment size classes lacks supporting data so far; there is some contrary evidence. Statistical association of alleles may cause estimates based on the assumption of statistical independence to understate the true matching probabilities by many orders of magnitude. The assumptions that DNA fragments occur independently and with constant frequency within a size class appear to be contradicted by the available data on the mean and variance of the number of fragments per person. The mistaken use of the geometric mean instead of the arithmetic mean to compute the probability that every DNA fragment of a randomly chosen person is present among the DNA fragments of a specimen may substantially understate the probability of a match between blots, even if other assumptions involved in the calculations are taken as correct. The conclusion is that some astronomically small probabilities of matching by chance, which have been claimed in forensic applications of DNA fingerprinting, presently lack substantial empirical and theoretical support.

Alleles↗
PubMed1990

Preparation of synthetic tandem-repetitive probes for DNA fingerprinting.

DNA fingerprints are generated using probes that hybridize to hypervariable minisatellites, also known as variable number tandem repeat loci. Cloned minisatellites have served as the predominant source of DNA fingerprinting probes. A short segment within the repeat units of minisatellites, called the "core" sequence, is highly conserved within a family of related minisatellites, thereby allowing a single-cloned minisatellite to cross-hybridize to 20 to 40 other minisatellites. In this article, we describe a method for the synthetic preparation of polymeric core sequence probes for DNA fingerprinting. Unlike "monomeric" oligonucleotide probes, the polymeric probes mimic the tandem-repetitive structure of minisatellites, and thus each probe molecule can potentially form many sites of hybridization with a target minisatellite. The synthetic probes are cloned into plasmid DNA to provide a perpetual source of probe material.

Base Sequence↗
PubMed1985

Genomic fingerprinting in the epidemiology of gonorrhoea.

We investigated the usefulness of the restriction enzyme (RE) fingerprinting for epidemiological tracing in gonococcal disease. The RE patterns of three paired gonococcal isolates showed corresponding identical fingerprints. Within each of the three pairs of epidemiologically linked isolates the respective restriction patterns were completely identical. Also, the restriction patterns of 6 strains from a larger contact group were identical. Identical restriction patterns were also obtained in each of the two cases where isolates were recovered from both urethra and cervix. The serological findings were in perfect agreement with the genomic fingerprinting as to the identity between all strains of the same epidemiologic chain. Relapse of the original infection could be excluded in one case by the finding of a different RE pattern and also a different serovar pattern of the strain recovered 4 months later.

DNA Restriction Enzymes↗
PubMed1989

[DNA fingerprinting of Rattus norvegicus: a new approach in genetic analysis].

Recent finding in highly effective DNA probes for RFLP testing (of hypervariable minisatellite DNA type) has led to the invention of DNA fingerprinting--the new technique of great value for identification of individuals, establishing biological kinship and studies in population genetics. We anticipate that DNA fingerprinting procedure with M13 phage DNA as a probe which we have developed earlier, makes it possible to apply new approach in genetic analysis--establishing, whether or not a particular locus is associated with the inheritance of genetic disease, by comparing the whole restriction fragment data from affected and unaffected animals. In this work, using the method described we characterized the Kroushynsky-Molodkina rat strain with hereditary disposition for epileptic attacks and performed comparative fingerprint analysis of these defective and normal rat genomes. The data obtained may hold some promises for further seeking the particular defective gene.

Animals↗
PubMed1989

Oligonucleotide fingerprint analysis of Japanese encephalitis virus strains of different geographical origin.

RNA fingerprint analysis was carried out with different strains of Japanese encephalitis virus which were isolated from Japan, China, India and Sri Lanka. From the similarity ratios, a similarity matrix was worked out which yielded a dendrogram. Geographical proximity of the place of isolation did not contribute much to the similarity of the fingerprint of the strains, nor did temporal proximity. The Japanese Nakayama strain had greater similarity with the Asansol strain from West Bengal. However, another strain from West Bengal, the Bankura strain, showed marked difference. Similarly the Bhopal and Beijing (China) strains were relatively close to each other while the Japanese JaGAr15460 strain was nearer to the strain from Gorakhpur. Serial mouse passage of the Asansol strain did not change the fingerprint pattern drastically.

China↗
PubMed1989

DNA fingerprinting by means of a nonradioactive probe of sulfonated DNA.

A DNA fingerprinting technique that uses a cold sulfonated DNA probe is described. DNA was extracted from blood samples of a family, who were subjected to DNA fingerprinting. The restriction fragments that were hybridized with the probe were colorimetrically detected by an enzyme immunoassay. For DNA detection and labeling a commercially available kit was employed. The sensitivity of this fingerprint detection method has been proven comparable to detection by the radioactive method, and hence is easier to use in conventional laboratories.

DNA↗
PubMed1988

Fingerprinting of proteins cleaved in solution by cyanogen bromide.

This paper describes a simple method for fingerprinting proteins cleaved in solution with cyanogen bromide (CNBr). Two modifications of standard solution CNBr digestion conditions facilitate routine application of solution digestion to multiple samples. First, proteins are recovered by neutralizing formic acid with N-ethylmorpholine and precipitating with acetone. This eliminates the need for lyophilization. Second, thiols are blocked by disulfide interchange. Disulfide interchange is simpler than reduction-alkylation because the reagent is inert and the excess need not be removed. We show how CNBr fingerprints of bovine serum albumin can be interpreted in terms of the amino acid sequence in ways that proteolytic fingerprints cannot.

Cyanogen Bromide↗
PubMed1986

Improved enhancement of ninhydrin developed fingerprints by cadmium complexation using low temperature photoluminescence techniques.

Fingerprints developed with ninhydrin form stable, colored complexes when treated with various metal salts. Many of these colored complexes can be used to increase the sensitivity of detection of latent prints because of photoluminescent properties. The intensity of this photoluminescence is increased at low temperature (77K), and this is a common characteristic of each of the complexes formed with salts of the IIb group of the Periodic Table. Spectral characteristics of these Group IIb metal complexes and the influence of environmental factors on their formation are reported. These data have helped determine optimal conditions for the enhancement of ninhydrin developed fingerprints. Taking into account spectral characteristics, solubility, versatility, stability, and reproducibility, the use of the cadmium nitrate tetrahydrate complex is advocated for general use for fingerprint enhancement. The use of zinc nitrate is favored if toxicological considerations are paramount, but ninhydrin development has to be carefully controlled if optimal results are to be obtained. Limited applications for mercuric complexes are found when a red shift is desired to remove background effects.

Cadmium↗
PubMed1984

Restriction endonuclease fingerprinting of meningococcal DNA.

We have successfully developed a restriction endonuclease fingerprinting (REF) technique for the study of meningococcal chromosomal DNA. A review of our application of this method in studies of meningococcal epidemiology and pathogenicity is given. By REF we could show that fingerprints of apparently identical B15 carrier strains were remarkably heterogeneous, whereas invasive B15 isolates possessed very similar fingerprints. We could also demonstrate REF heterogeneity among B15 isolates collected from cases with meningococcal disease and their close contacts during an outbreak of meningococcal disease in a military camp in North Norway. The REF technique has also proved valuable for our studies on meningococcal piliation and adherence. At present, we are studying 67 different meningococcal isolates collected from all parts of Norway during the MenOPP project.

Centrifugation↗
PubMed1981

Comparison of RNase T1 fingerprints of U1, U2, and U3 small nuclear RNA's of HeLa cells, human normal fibroblasts, and Novikoff hepatoma cells.

To determine whether there are differences between the U1, U2, and U3 small nuclear RNA's of human cancer cells (HeLa cells) and human normal fibroblasts (IMR-90 cells), and between these uridine-rich small nuclear RNA's of human and Novikoff hepatoma cells, the cells were first incubated in Eagle's medium with [32P]Pi to label these RNA's uniformly. No differences were found between the RNase T1 fingerprints of the purified U1, U2, and U3 RNA's of HeLa cells and IMR-90 cells. The RNase T1 fingerprints of U1 RNA's from human tissues were very similar to that of the U1 RNA of Novikoff hepatoma cells. The RNase T1 fingerprints of U2 and U3 RNA's from human tissues had many similarities to those of Novikoff hepatoma cells, but a few differences were found, such as a point mutation of the U-U-Gp in the rat U2 RNA to A-U-Gp (U leads to A) in human U2 RNA. Unlike the three U3 RNA's of Novikoff hepatoma cells, U3 RNA from human tissues appears to be only one species. These results indicate that U1, U2, and U3 RNA's of human cancer cells are essentially the same as those of human normal cells. In addition, the uridine-rich small nuclear RNA's appear to be conserved through evolution.

Animals↗
PubMed1980

Multiparameter appraoch to the "fingerprinting" of fluorescent Pseudomonads.

A recent study has shown that an emission-excitation matrix could be used to provide a unique fingerprint for the selective identification and characterization of certain species of Pseudomonas. This paper describes the results of a systematic study of the variables that contribute to the uniqueness of the fingerprint. Additional insight concerning the nature of the fluorescent pigments of these species is gained by analysis with a high-performance liquid chromatograph/video fluorometer combination. While providing information about the analytical variables contributing to the analysis, this combination also added the variable of chromatographic retention time for unambiguous fingerprinting.

Autoanalysis↗