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Digestion characteristics of beef steers fed silage and different levels of energy with or without protein supplementation.

Six large-framed crossbred steers averaging 262 kg were used in a 6 x 7 incomplete Latin square design to study the effect of feeding a source of readily available energy (molasses) in combination with highly degradable protein (canola meal) on ruminal characteristics, digestibility, and in situ disappearance of DM and N. The seven dietary treatments were offered for ad libitum consumption and consisted of 1) timothy silage alone or mixed (on a DM basis) with 2) 7.5% molasses, 3) 15% molasses, 4) 7.5% canola meal, 5) 5.5% canola meal and 7.5% molasses, 6) 3.6% canola meal and 15% molasses, or 7) 15% canola meal. The total N content of supplements containing 7.5% canola meal, 5.5% canola meal and 7.5% molasses, and 3.6% canola meal and 15% molasses was similar. Molasses supplementation decreased (P < .05) ruminal ammonia N concentration and digestibility of energy and fiber. Canola meal supplementation increased (P < .05) N and energy digestibility but decreased ADF digestibility compared with feeding unsupplemented timothy silage. Feeding 15% compared with 7.5% canola meal increased (P = .05) DM digestibility and tended (P = .07) to increase energy digestibility. Combining 7.5% molasses and 5.5% canola meal did not improve DM digestibility, but adding 15% molasses decreased (P < .05) energy and fiber digestibility of the diet, which may explain lower ADG obtained for beef steers fed such levels of molasses in other research.

Animals↗

The effect of fat inclusion on diet digestibility in growing rabbits.

Fifty-six California x New Zealand rabbits were used to study the influence of fat addition on nutrient digestibility. Seven diets were formulated to a 24% ADF level: Diet C had no added fat; Diets T, O, and S contained 3% of added beef tallow, oleins, and soybean oil, respectively; and Diets TWS, OWS, and SWS contained the same level and types of fat but also 18% heated whole soybean meal (WS) to increase dietary ether extract (EE) by 3%. After the digestibility trial, rabbits (weighing 2.00 +/- .13 kg) were slaughtered to determine the composition of cecal contents. Fat addition increased digestibility of energy (P < .05) and EE (P < .001). The type of fat only affected (P < .05) EE digestibility. No interaction between fat types and WS was found. Although EE levels of cecal contents increased in diets with added fat (P < .001), they did not negatively affect dietary fiber digestibility. The patterns of fatty acid digestibility in Diet C and in those with soybean oil (S and SWS) were similar; the lowest values were obtained for C16:0 and C18:0. The digestibility of unsaturated fatty acids of C18 increases as its degree of unsaturation increases. Digestibilities of C16:0 and C18:2 were, respectively, lower and higher in the diet with 3% soybean oil than in the diet with 3% tallow.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed↗

Effects of chemically treated, recycled newsprint on feed intake and nutrient digestibility by growing lambs.

In situ experiments and two lamb digestion trials were conducted to evaluate the effects of chemically treating recycled newsprint (NP). Treatment of NP with 2% HCl (percentage of NP DM) followed by autoclaving for 4 h was most efficacious and was used in an in vivo digestion trial with 25 ram and wether lambs in a randomized complete block design (RCB). On a DM basis treatment diets were: 1) control, 95% alfalfa hay (AH); 2) 75% AH, 20% HCl-treated NP (HCl-NP); 3) 55% AH, 40% HCl-NP; 4) 75% AH, 20% untreated NP; and 5) 55% AH, 40% untreated NP on a DM basis. Lambs fed Treatments 2 and 3 had ad libitum DMI (1,043 and 1,036 g/d, respectively) similar to the DMI of those fed the control treatment (1,024 g/d); however, lambs fed Treatments 4 and 5 had lower (P < .05) ad libitum DMI (578 and 426 g/d, respectively) than lambs fed Treatments 1, 2, and 3. Apparent DM digestibilities were lower (P < .05) by lambs consuming Treatments 2, 3, 4, and 5 (52.6, 44.5, 52.0, and 48.2%, respectively) than by those consuming Treatment 1 (60.7%). Apparent OM, CP, NDF, and ADF digestibilities followed trends similar to DM. In the second digestion trial, 15 ram and wether lambs were used in a RCB to compare Treatments 1, 2, and 3. For this second digestion study, NP was treated with 4% HCl (percentage of NP DM), autoclaved for 4 h, and diets were fed at 2% of BW. As in Trial 1, apparent nutrient digestibilities decreased (P < .05) with increasing amounts of HCl-NP. Our results suggest that HCl-NP can be fed to sheep at 40% of the diet without a decrease in DMI; however, apparent nutrient digestibility decreases with increasing levels of HCl-NP.

Animal Feed↗

Effect of dietary neutral detergent fiber on ileal digestibility and portal flux of nitrogen and amino acids and on nitrogen utilization in growing pigs.

Two experiments were conducted to determine the effects of dietary NDF on apparent ileal and fecal digestibility and portal flux of nitrogen (N) and amino acids, and on N retention in growing pigs. In four equal portions (at 0600, 1200, 1800, and 2400) barrows on Treatment B received a basal diet, based on casein, cornstarch, and dextrose, at a feeding level of 2.6 times energy for maintenance. Barrows on Treatment B+NDF received an additional amount of 15% (wt/wt) of purified wheat bran NDF (pNDF). In Exp. 1, four ileally cannulated barrows (40 to 75 kg) were used in a crossover arrangement comprising two treatments and three periods. The addition of pNDF decreased ileal N digestibility from 94.1 to 88.9% (P < .001), whereas ileal digestibility of most amino acids was 2 to 5.5 percentage units lower (P < .001). Utilization of ileally digested N increased from 64 to 72% with the addition of pNDF, presumably because of the contribution of pNDF to the energy supply. In Exp. 2, three barrows (30 to 54 kg) fitted with catheters in the portal vein and the mesenteric vein and artery were used in a crossover arrangement comprising two treatments and five periods. Portal absorption of nutrients was derived by multiplying the porto-arterial plasma concentration differences by portal vein plasma flow. The pNDF did not significantly affect the absorption of ileally digested amino acids and the portal flux of ammonia and urea. The results showed that addition of NDF reduced amino acid digestibility, but not the portal flux of digested amino acids, and NDF energy presumably improved utilization of ileally digested amino acids.

Amino Acids↗

Supplemental cracked corn for steers fed fresh alfalfa: I. Effects on digestion of organic matter, fiber, and starch.

The effect of supplementation with different levels of cracked corn on the sites of OM, total dietary fiber (TDF), ADF, and starch digestion in steers fed fresh alfalfa indoors was determined. Six Angus steers (338 +/- 19 kg) fitted with cannulas in the rumen, duodenum, and ileum consumed 1) alfalfa (20.4% CP, 41.6% NDF) ad libitum (AALF); 2), 3), and 4) AALF supplemented (S) with .4, .8, or 1.2%, respectively, of BW of corn; or 5) alfalfa restricted at the average level of forage intake of S steers (RALF), in a 5 x 5 Latin square design. Total OM intake was lower (P < .01) in steers fed RALF than in those fed AALF but level of forage intake did not affect sites of OM, TDF, or starch digestion (P > .05). Forage OM intake decreased (P < .01) linearly (8,496 to 5,840 g/d) but total OM intake increased (P = .03) linearly (8,496 to 9,344 g/d) as corn increased from .4 to 1.2% BW. Ruminal apparent and true OM disappearance was not affected, but OM disappearing in the small intestine increased (P < .01) linearly with increasing levels of corn. Total tract OM digestibility (71.2 to 76.2%) and the proportion of OM intake that was digested in the small intestine (15.4 to 24.5%) increased (P < .01) linearly as corn increased. The TDF and ADF intakes decreased (P < .01) linearly as level of corn increased. Total tract TDF and ADF digestibilities were not different among treatments (average 62.9 and 57.8%, respectively). Starch intake and starch digested in the rumen and small and large intestine increased (P < .01) linearly with increasing corn level. Ruminal pH and VFA concentrations decreased and increased (P < .01), respectively, with increasing corn. Supplementation with corn increased OM intake, decreased forage OM intake, and increased the proportion of OM that was digested in the small intestine, but fiber digestion was not affected.

Animals↗

Effects of corn processing and dietary fiber source on feedlot performance, visceral organ weight, diet digestibility, and nitrogen metabolism in lambs.

In Exp. 1, early-weaned Targhee and Polypay crossbred lambs (60 ewes and 66 rams; initial BW 24 +/- 1.0 kg) were used in a 2 x 3 factorial experiment to determine the effects of corn processing (whole shelled corn [WSC] or ground and pelleted corn [GC]) in combination with supplemental fiber (none [control]; soybean hulls, SBH [highly digestible]; or peanut hulls, PH [highly indigestible]) on DMI, ADG, feed efficiency, and visceral organ weight. For the total trial, WSC resulted in a 4% increase (P < .01) in ADG vs GC, and supplemental fiber resulted in increased (P < .01) DMI and ADG vs the control diet. Experiment 2 was conducted using 12 Targhee and Polypay crossbred wether lambs (initial BW 25 +/- 7 kg) to determine the effects of corn processing and fiber source in high-concentrate diets on diet digestibility and N retention using the same diets as in Exp. 1. Lambs fed WSC had greater (P < .001) apparent N digestion, true N digestion, and N retention (P < .01) than those fed GC. The apparent digestibilities of DM, OM, and NDF were greater (P < .001) for WSC than for GC diets. Peanut hulls resulted in decreased (P < .01) DM, OM, and NDF apparent digestibilities compared with the control and SBH diets. Starch digestion was not affected (P > .10) by diet. Whole corn resulted in improved DM, OM, NDF, and N digestibility compared with GC. Overall, both the SBH and PH diets resulted in greater DMI and ADG than the control diet, which lacked supplemental fiber.

Animal Feed↗

Brown midrib-3 corn silage improves digestion but not performance of growing beef steers.

The brown midrib-3 (bm3) gene mutation has been incorporated into corn plants to potentially improve fiber digestibility. The objectives of this study were to determine the effect of bm3 corn silage on digestion and performance of growing beef steers and to determine whether limiting intake would further enhance fiber digestibility of bm3 corn silage. A bm3 hybrid and its isogeneic normal counterpart were harvested at three-quarters kernel milk line. Neutral detergent fiber, ADF, and ADL were 4.5, 6.9, and 1.9 units lower, respectively, and DM was 5.4 units higher for bm3 than for normal silage. In Trial 1, eight ruminally fistulated Angus crossbred steers (224 +/- 24 kg) were randomly assigned to a 2 x 2 factorial arrangement of treatments in a replicated 4 x 4 Latin square design. Steers had ad libitum feed access or were restricted to 80% of ad libitum intake of diets containing 86% normal corn silage (Control) or bm3 corn silage (BMCS). The remainder of the diets consisted of soybean meal, urea, monensin, vitamins, and minerals. Dry matter intake was greater (P < 0.01) for steers offered ad libitum access to BMCS than for those with ad libitum access to the Control diet. The BMCS treatment resulted in improved (P < 0.05) apparent total-tract digestibility of DM, OM, NDF, and ADF. Mean concentration of total VFA and molar proportions of acetate were increased (P < 0.05) by feeding BMCS. There tended to be a DMI x hybrid interaction (P = 0.16) for apparent total-tract digestibility of NDF. When diets were offered ad libitum, BMCS increased NDF digestibility by 10.5 percentage units compared with Control, but, when DMI was limited, BMCS increased NDF digestibility by 15.8 percentage units. In Trial 2, 128 steer contemporaries of those used in Trial 1 (245 +/- 13 kg) were offered ad libitum access to BMCS or Control diets as used in Trial 1. After a 112-d treatment period, concentrate in the diet was increased, and all steers were fed a common finishing diet. During the 112-d treatment period, steers receiving BMCS consumed 0.45 kg more DM/d (P < 0.05) and had similar ADG (P > 0.10), compared with those steers receiving the Control silage. This resulted in poorer (P < 0.01) feed efficiency for steers receiving BMCS. Finishing phase and overall performance of the steers was not different (P > 0.10) due to treatment. Although feeding BMCS in growth-phase diets resulted in increased daily DMI and improved digestibility of DM and fiber, it did not result in improved steer feedlot ADG compared with Control silage.

Animal Feed↗

Pretanned leather shavings in a supplement mixture for steers: I. In situ and in vitro disappearance, ruminal fermentation, and organic matter, nitrogen, and fiber digestion.

Two digestion studies were conducted to evaluate the use of pretanned leather shavings as a component of a protein supplement. In Exp. 1, the in situ and in vitro disappearance of pretanned leather shavings and soybean meal was evaluated. Results revealed that less than 18.4% of the pretanned leather shavings was solubilized and disappeared when exposed to McDougall's buffer for 48 h, but there was 90.0% disappearance with 48-h exposure to a .1 N HCl/pepsin treatment and 97.0% disappearance with exposure to a two-stage digestion. In situ disappearance following 72 h in the rumen allowed 6.8% disappearance. Thus, leather shavings seem to be relatively indigestible in the rumen, but postruminal digestion may be possible. In Exp. 2, six Angus x Holstein steers, fitted with ruminal and duodenal cannulas, were used in a replicated 3 x 3 Latin square to evaluate ruminal and digestion effects of the following supplements combined with fescue hay at 1.7% of BW (DM basis): no supplementation (control); supplementation intraruminally with soybean meal at .07% of BW (as-fed basis); and supplementation intraruminally with a combination of soybean meal and pretanned leather shavings (17:8 ratio) at .05% of BW (isonitrogenous to soybean meal; as-fed basis). Ruminal fluid passage rate was greater and fluid turnover time was shorter in steers fed leather shavings than in those fed soybean meal (P = .10). Ruminal pH was lower (P = .04) for supplemented steers than for control steers and ruminal NH3 N concentration was greater (P = .01) in steers fed soybean meal than in those fed leather shavings. Total VFA concentration was increased (P = .02) by supplementation. Supplementation with soybean meal increased (P < .05) ruminal molar proportions of butyrate, valerate, and isovalerate compared with leather shavings. Duodenal OM flow and OM disappearing in the intestines were increased by supplementation (P < .10), but not by the type of supplement fed (P > .10). Ruminal digestion of OM and total tract OM digestion were unaffected (P > .10) by supplementation and the type of supplement fed. Flow and digestion of NDF were unaffected (P > .10) by the treatments. Flow of N and the quantity of N disappearing in the intestines were increased (P < .05) by supplementation but did not differ (P > .10) between supplementation groups. Microbial N flow, N utilization for net microbial protein synthesis, and ruminal N disappearance were unaffected (P > .10) by supplementation and the type of supplement provided. Combining pretanned leather shavings with soybean meal seemed to have no deleterious effects on digestion or fermentation and to allow for escape of some N to the lower tract.

Animal Feed↗

In vitro growth and starch digestion by Entodinium exiguum as influenced by the presence or absence of live bacteria.

In a preliminary study, the addition of antibiotics was shown to reduce bacterial concentrations in Entodinium exiguum cultures by more than 99% in 4 h, whereas the protozoal population was apparently unaffected. Using this procedure, the growth and amylolytic capability of Entodinium exiguum, in the presence or absence of live bacteria, was studied in vitro. Treatments for Trial 1 were protozoa plus antibiotics (PA), PA plus autoclaved bacteria (PAB), protozoa plus living bacteria (PLB), and only bacteria (BAC). Autoclaved or non-autoclaved cornstarch was used as an energy source. Treatment main effects were as follows: higher concentration of E. exiguum in PLB than in PA or PAB at 24 and 48 h (P < 0.01); PA and PAB were not different (P > 0.05); concentrations of E. exiguum higher in autoclaved cornstarch at 12 h (P < 0.05) but lower than in non-autoclaved cornstarch at 24 and 48 h (P < 0.01); and starch digestion in PLB was higher than in PA and PAB at all time periods, but only greater than BAC up to 24 h (P < 0.01). In Trial 2, only treatments PA, PLB, and BAC were tested. Rice starch and cornstarch were used as substrates. With rice starch, growth was higher in PLB than in PA at 24 and 48 h (P < 0.05). Starch digestion started earlier in PLB with rice starch (P < 0.05) but was complete for both substrates after 24 h. Up to 12 h (autoclaved cornstarch and rice starch) and 24 h (non-autoclaved cornstarch and cornstarch), the sum of digestion by bacteria and protozoa did not equal the extent of digestion in PLB, suggesting some kind of synergism. Total extent of digestion with protozoa was similar between the two sources; however, bacteria digested rice starch faster and to a greater extent than cornstarch. Approximate lag times with rice starch, autoclaved cornstarch, and non-autoclaved cornstarch were 6, 3, and 12 h for bacteria and < 6, 3, and 9 h for protozoa, respectively. Rate of digestion for non-autoclaved cornstarch was similar for bacteria and protozoa, whereas the rate of bacterial digestion was much faster with the other two substrates (autoclaved cornstarch and rice starch).

Animals↗

Impact of EasiFlo cottonseed on feed intake, apparent digestibility, and rate of passage by goats fed a diet containing 45% hay.

EasiFlo cottonseed (ECS), produced by coating whole cottonseed (WCS) with cornstarch to simplify handling and mixing with other ingredients, is marketed commercially. The objective of this trial was to determine its digestibility by small ruminants. Four mature Nubian wether goats, in a 4 x 4 Latin square arrangement of treatments, were fed diets that contained about 45% bermudagrass hay (BGH) plus 0, 15.7, 32.7, or 50.3% ECS, with the ECS replacing corn and soybean meal (2:1 ratio) in the concentrate portion of the diet. Feed intakes and digestibility of components were measured, and passage rate was estimated using ytterbium-marked BGH. Dry matter intake decreased at an increasing rate (P < 0.01) as ECS or fat concentration in the diet increased. Digestibility was linearly depressed (P= 0.003) as ECS replaced corn and soybean meal in the diet, primarily due to depressed (P < 0.05) digestibility of NDF, ADF, and nonfibrous carbohydrates (NFC). In contrast, fat digestibility tended to increase (P = 0.11) linearly and N utilization was increased (P = 0.04) linearly as ECS concentration was increased. Passage kinetics were not altered. Based on regression estimates of TDN for BGH and literature values of TDN for other feed ingredients, the total digestible nutrient content of ECS for mature goats fed a 45% roughage diet was estimated to be 78.0 +/- 9.1%, a value quite similar to that (77.2%) proposed for whole cottonseed for large ruminants by NRC tables. Low digestibility of fiber (under 10% of NDF) and of nonfibrous carbohydrate limits the digestibility of DM and energy from ECS.

Animal Feed↗

Ileal amino acid digestibilities by pigs fed soybean meals from five major soybean-producing countries.

Growing conditions and processing technologies to which soybeans (SB) are exposed have an effect on digestibilities of AA found in the resultant soybean meals (SBM). This study evaluated SBM from five major SB-producing countries (Argentina, Brazil, China, India, and the United States). An industry representative in each country collected samples of unprocessed SB and SBM subjectively determined to be of high, intermediate, or low quality. The SB from each country were processed into SBM under uniform conditions in the United States. Five experiments (each examining the three SBM and the SB processed in the United States from a single country) were conducted to determine true ileal AAd digestibilities. In addition, a standard SBM purchased on the open market in the United States was used in all experiments as a control. Data from pigs fed a low-protein casein diet in each study were used to calculate true AA digestibilities. Pigs were fitted with simple T-cannulas at the terminal ileum and allotted to treatments in Latin square design experiments. Duplicate experiments were conducted at the University of Illinois and at The Ohio State University. Within each country comparison, pigs fed the SBM processed in the U.S. from SB grown in the five countries had lower (P < 0.05) true total amino acid (TAA) digestibilities than did pigs fed any of the SBM prepared within the country of origin, except the United States. This indicates that processing conditions used at the U.S. pilot plant were not ideal when using SB from other countries. True TAA digestibilities of the diets containing the high-, intermediate-, and low-quality SBM did not differ, except for China, where the low-quality SBM (83.5%) had a lower (P < 0.05) digestibility than the intermediate- (89.6%) or high- (89.0%) quality meals. Soybean meal produced in Argentina (average, 87%) and Brazil (average, 82%) had lower (P < 0.05) true TAA digestibilities than did the standard SBM (91%), indicating that the processing plants in those countries may produce a less digestible SBM than that available on the open market in the United States.

Agriculture↗

Prediction of nutritive values in grass silages: I. Nutrient digestibility and energy concentrations using nutrient compositions and fermentation characteristics.

Grass silages (n = 136) were selected from commercial farms across Northern Ireland according to their pH, ammonia nitrogen, DM, and predicted ME concentration. Each silage was offered to four sheep as a sole feed at maintenance feeding level to determine nutrient digestibility and urinary energy output. Dry matter concentration was determined as alcohol-corrected toluene DM and was subsequently used as the basis for all nutrient concentrations. The objectives were to use these data to examine relationships between nutritive value and nutrient concentration or fermentation characteristics in silages and then develop prediction equations for silage nutritive values using stepwise multiple regression techniques. The silages had a large range in quality (DM = 15.5 to 41.3%, ME = 7.7 to 12.9 MJ/kg of DM, pH = 3.5 to 5.5) and a relatively even distribution over the range. There was a positive relationship (P < 0.001) between silage GE and DE or ME concentration. Digestible OM in total DM (DOMD); ME/GE; and digestibility of DM, OM, and GE were positively related (P < 0.05) to CP, soluble CP, ether extract, lactic acid concentration, and lactic acid/ total VFA, whereas they were negatively related (P < 0.05) to ADF, NDF, lignin, individual VFA concentration, pH, and ammonia N/total N. Concentrations of DE and ME and digestibility of CP and NDF had similar relationships with those variables, although some relationships were not significant. Three sets of multiple prediction equations for DE and ME concentration; ME/ GE; DOMD; and digestibility of DM, OM, GE, CP, and NDF were therefore developed using three sets of predictors. The first set included GE, CP, soluble N/total N, DM, ash, NDF, lignin, lactic acid/total VFA, and ammonia N/total N; the second set excluded soluble N/ total N and lignin because they are not typically measured; the third set further excluded the fermentation data. The R2 values generally decreased with exclusion of predictors. The second and third sets of equations, except for NDF digestibility, were validated using the mean-square-prediction-error model and an independent grass silage data set published since 1977 (n = 17 [DM digestibility] to 28 [DOMD and OM digestibility]). The validation indicated that the equations developed in the present experiment could accurately predict DE and ME concentrations and DE/GE and ME/GE in grass silages.

Animal Feed↗

Effects of spray-dried animal plasma on intake and apparent digestibility in dogs.

Effects of spray-dried animal plasma (SDAP) on intake and apparent digestibility of major dietary components were determined using 22 adult Beagles. Trials 1 and 2 used six and eight dogs, respectively, in a switchback design using 10-d periods. Trial 3 used eight dogs in a replicated 4 x 4 Latin square design with 15-d periods. The final 5 d of each period were used for measurement of intake and fecal collections. In Trial 1, dry extruded dog food kibbles were coated with 5% tallow, 2% commercial flavor, and 0 or 2% SDAP (as-fed basis). In Trial 2, commercially available dry dog food, previously coated with fat and flavor were coated with 0 or 2% SDAP. In Trial 3, SDAP (0, 1, 2, or 3%) was blended with other ingredients and extruded (as-fed basis). Kibbles were subsequently coated with 5% poultry fat and 1% commercial flavor. Intake, fecal consistency, and apparent digestibility of nutrients were determined. Addition of SDAP did not markedly affect chemical composition of diets and did not affect intake. Digestibility of DM was improved (P < 0.04) an average of 3.2% when 2% SDAP was included in the diet for all trials. Organic matter digestibility was improved (P < 0.01) in Trials 2 and 3 by an average of 2.9%. Also, digestibility of crude fiber (Trials 1 and 2) or total dietary fiber (Trial 3) was increased with addition of SDAP to the diet (P < 0.01). Fecal DM excretion was decreased by an average of 15% across all trials with the addition of SDAP. Spray-dried animal plasma was an acceptable ingredient in dry dog food preparations, resulting in improved digestion and decreased fecal output. Changes in digestion that occurred with addition of SDAP suggested alteration in digestive capacity in dogs.

Animal Feed↗

Effects of emulsification, fat encapsulation, and pelleting on weanling pig performance and nutrient digestibility.

Two experiments were conducted to evaluate the effect of lysolecithin on performance and nutrient digestibility of nursery pigs and to determine the effects of fat encapsulation by spray drying in diets fed in either meal or pelleted form. In Exp. 1, 108 pigs (21 d of age; 5.96 +/- 0.16 kg BW) were allotted to one of four dietary treatments (as-fed basis): 1) control with no added lard, 2) control with 5% added lard, 3) treatment 2 with 0.02% lysolecithin, and 4) treatment 2 with 0.1% lysolecithin in a 35-d experiment. Added lard decreased ADG (P = 0.02) and ADFI (P < 0.06) during d 15 to 35 and overall. Lysolecithin improved ADG linearly (P = 0.04) during d 15 to 35 and overall, but did not affect ADFI or G:F. Addition of lard decreased the digestibility of DM (P = 0.10) and CP (P = 0.05) and increased (P = 0.001) fat digestibility when measured on d 10. Lysolecithin at 0.02%, but not 0.10%, tended to improve the digestibility of fat (P = 0.10). On d 28, digestibilities of DM, fat, CP, P, (P = 0.001), and GE (P = 0.03) were increased with the addition of lard, and lysolecithin supplementation linearly decreased digestibilities of DM (P = 0.003), GE (P = 0.007), CP, and P (P = 0.001). In Exp. 2, 144 pigs (21 d of age, 6.04 +/- 0.16 kg BW) were allotted to one of six treatments in a 3 x 2 factorial randomized complete block design. Factors included 1) level (as-fed basis) and source of fat (control diet with 1% lard; control diet with 5% additional lard; and control diet with 5% additional lard from encapsulated, spray-dried fat) and 2) diet form (pelleted or meal). Addition of lard decreased feed intake during d 0 to 14 (P = 0.04), d 15 to 35 (P = 0.01), and overall (P = 0.008), and improved G:F for d 15 to 35 (P = 0.04) and overall (P = 0.07). Encapsulated, spray-dried lard increased ADG (P = 0.004) and G:F (P = 0.003) during d 15 to 28 compared with the equivalent amount of fat as unprocessed lard. Pelleting increased ADG (P = 0.006) during d 0 to 14, decreased feed intake during d 15 to 35 (P = 0.01), and overall (P = 0.07), and increased G:F during all periods (P < 0.02). Fat digestibility was increased (P = 0.001) with supplementation of lard, and this effect was greater when diets were fed in meal form (interaction, P = 0.004). Pelleting increased the digestibility of DM, OM, and fat (P < 0.002). Results indicate that growth performance may be improved by lysolecithin supplementation to diets with added lard and by encapsulation of lard through spray drying.

Animal Feed↗

Chemical composition, protein quality, palatability, and digestibility of alternative protein sources for dogs.

The chemical composition and protein quality of 11 alternative protein sources (chicken products, blood products, enzyme-hydrolyzed fish protein concentrate, soybean meal, and spray-dried pork liver) were determined, and an experiment was conducted to determine palatability and digestibility of processed red blood cell-containing diets. Chicken protein sources differed in concentrations of CP, acid-hydrolyzed fat, and total AA (TAA) by 20, 31, and 24%, respectively, and GE by 1.7 kcal/g. Blood protein sources varied little in acid-hydrolyzed fat and GE concentrations, but concentrations of CP and TAA differed by 11 and 8%, respectively. Protein solubility of chicken and blood protein source categories averaged 57 and 69%, respectively. Protein solubility of enzyme-hydrolyzed fish protein concentrate, soybean meal, and spray-dried pork liver was 53, 67, and 26%, respectively. Based on calculations from immobilized digestive enzyme assay values, lysine digestibility averaged approximately 80.4 and 81.7% for blood and chicken protein sources, respectively. Lysine digestibility values for soybean meal and spray-dried pork liver were 89 and 77%, respectively. A chick protein efficiency ratio (PER) assay showed that chicken protein sources had high protein quality values, as the PER ranged from 2.7 to 5.3, whereas blood protein sources had poor protein quality (PER values less than 1.5). Enzyme-hydrolyzed fish protein concentrate, spray-dried pork liver, and soybean meal had high protein quality (PER values greater than 2.8). In the dog palatability and digestibility experiments, a corn and chicken-based diet supplemented with either 0 or 3% processed red blood cells was tested. The palatability test showed that dogs consumed more of the diet that contained 0% vs. 3% processed red blood cells. The intake ratio for the 3% processed red blood cells diet was 0.34. Nutrient digestibilities did not differ, except for CP, where the digestibility was greater (P = 0.01) for dogs consuming the 0% processed red blood cells diet. These data suggest that chemical composition and quality of alternative protein sources differ greatly among ingredients within the same category. Palatability data suggest that a processed red blood cells-containing diet is not highly palatable but, when this diet was offered as only one food, dogs demonstrated no aversion response but some decrease in protein digestion.

Animal Feed↗

Effect of graded doses and a high dose of microbial phytase on the digestibility of various minerals in weaner pigs.

An experiment with 224 weaner pigs (initial BW of 7.8 kg) was conducted to determine the effect of dose of dietary phytase supplementation on apparent fecal digestibility of minerals (P, Ca, Mg, Na, K, and Cu) and on performance. Four blocks, each with 8 pens of 7 pigs, were formed. Eight dietary treatments were applied to each block in the 43-d experiment: supplementation of 0 (basal diet), 100, 250, 500, 750, 1,500, or 15,000 phytase units (FTU) or of 1.5 g of digestible P (dP; monocalcium phosphate; positive control) per kilogram of feed. The basal diet, with corn, barley, soybean meal, and sunflower seed meal as the main components, contained 1.2 g of dP per kilogram of feed. Fresh fecal grab samples were collected in wk 4 and 5 of the experiment. Average daily feed intake, ADG, G:F, and digestibility of all of the minerals increased (P < 0.001) with increasing phytase dose. Digestibility of P increased from 34% in the basal diet to a maximum of 84% in the diet supplemented with 15,000 FTU, generating 1.76 g of dP per kilogram of feed. At this level, 85% of the phytate phosphorus was digested, compared with 15% in the basal diet. Compared with the basal diet, digestibility of the monovalent minerals increased maximally at 15,000 FTU, from 81 to 92% (Na) and from 76 to 86% (K). In conclusion, phytase supplementation up to a level of 15,000 FTU/kg of a dP-deficient diet improved performance of weaner pigs and digestibility of minerals, including monovalent minerals. Up to 85% of the phytate-P was digested. Thus, dietary phytase supplementation beyond present day standards (500 FTU/kg) could further improve mineral use and consequently reduce mineral output to the environment.

6-Phytase↗

Evaluation of the inclusion of soybean oil and soybean processing by-products to soybean meal on nutrient composition and digestibility in swine and poultry.

This experiment was designed to evaluate the effects of selected soybean (SB) processing byproducts (gums, oil, soapstock, weeds/trash) when added back to soybean meal (SBM) during processing on the resulting nutrient composition, protein quality, nutrient digestibility by swine, and true metabolizable energy (TMEn) content and standardized AA digestibility by poultry. To measure ileal DM and nutrient digestibility, pigs were surgically fitted with a T-cannula in the distal ileum. The concentration of TMEn and the standardized AA digestibility by poultry were determined using the precision fed cecectomized rooster assay. Treatments in the swine experiment included SBM with no by-products; SBM with 1% gum; SBM with 3% gum; SBM with 0.5% soapstock; SBM with 1.5% soapstock; SBM with 2% weeds/trash; SBM with a combination of 3% gum, 1.5% soapstock, and 2% weeds/trash; SBM with 5.4% soybean oil; and roasted SB. A 10 x 10 Latin square design was utilized. The experiment was conducted at the University of Illinois, Urbana-Champaign, and at The Ohio State University, Columbus. In the swine experiment, apparent ileal DM, OM, CP, and AA digestibilities were reduced (P < 0.05) when pigs consumed the combination by-product diet compared with the diet containing no by-products. Apparent ileal digestibilities of DM, CP, and total essential, total nonessential, and total AA were lower (P < 0.05) for any diet containing by-products compared with the diet with no by-products. Apparent ileal digestibilities of DM, OM, CP, and AA were lower (P < 0.05) for the roasted SB-compared with the SB oil-containing diet. In the rooster experiment, TMEn values were greater (P < 0.05) for roasted SB compared with SBM with no by-products and increased linearly as the addition of soapstock increased. Individual, total essential, total nonessential, and total AA digestibilities were lower (P < 0.05) for roosters fed roasted SB versus SBM devoid of by-products. Gums, soapstock, and weeds/trash reduce the nutritive value of the resultant meal when they are added back during processing.

Animal Feed↗

Effects of monensin on site and extent of whole corn digestion and bacterial protein synthesis in beef steers.

Monensin was fed at 0 and 33 ppm in a crossover trial designed to determine the effect of this compound on ruminal and postruminal utilization of a corn-based diet and bacterial protein synthesis in abomasally fistulated steers. Monensin decreased (P greater than .10) ruminal true digestion of organic matter (OMc, corrected for bacterial cell synthesis) and apparent ruminal digestion of starch by 19%, but had not effect on apparent total tract digestion of OM or starch. Apparent ruminal and total tract digestibilities of crude protein (CP) were unchanged. Monensin decreased (P greater than .07) the contribution of bacterial N to total abomasal N (52 vs 58%), and increased (P greater than .06) the contribution of ruminally undegraded feed N (46 vs 40%), but had not effect on total N or amino acids recovered from the abomasum. Efficiency of bacterial protein synthesis (grams bacterial CP/100 g ruminally digested OMc) was unchanged. Monensin decreased (P greater than .05) the fraction of bacterial N to total N digested postruminally (42 vs 50%) and increased (P greater than .05) the contribution of ruminally undegraded feed N digested postruminally (58 vs 50%). Monensin caused a greater proportion of feed N and starch to be digested in the intestines than in the rumen (with possibly greater resultant metabolic efficiency), and this may account for some of the benefits obtained from feeding this compound with high grain diets.

Abomasum↗