Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “image analysis”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 811 records · Page 45Linked to original sources
PubMed2003

Automated image analysis of proliferating cells in carcinoma of the larynx.

OBJECTIVE: To determine the role of cell proliferation neoplastic progression in the larynx and possibly derive criteria of clinical significance using automated quantitative image analysis. MATERIAL AND METHODS: In a retrospective study involving archival material, the occurrence and location, size, shape and staining intensity of proliferating cell nuclear antigen (PCNA)-positive cells (12,538 cells in total) were analyzed in squamous cell carcinoma (SCC), as well as in pre- and non-neoplastic conditions, using computer-assisted morphometry with reproducibility and sensitivity exceeding 99%. RESULTS: Immunohistochemically detectable PCNA-positive cells were located in the basal layer in non-neoplastic states, in well-differentiated SCCs in layers adjacent to the basal membrane and in poorly differentiated neoplasms in the neoplastic epithelial islets. An increased degree of dysplasia was associated with an increased number of PCNA-immunoreactive cells of increased nuclear size and staining intensity. There was a significant difference between carcinomas and dysplasia in terms of altered nuclear shape. With increasing malignancy of SCCs, nuclear shape alterations and PCNA staining intensity increased, whilst nuclear size decreased. CONCLUSIONS: Automated image analysis of cell populations allowed the identification of populations of malignant cells and provided information on the severity of preneoplastic and neoplastic conditions of use in studies of tumor behavior and with potential clinical application.

Carcinoma, Squamous Cell↗
PubMedDate not supplied

[Is detection of schizocytes by computerised image analysis accurate?].

Schistocytes result from red cell fragmentation. The identification of the schistocytes is critical for decisions on appropriate management of the patients. Currently, a systematic approach to the counting method remains rewarded. We programmed a computer image analysis device (Q-Win, Leica) in order to detect fragmented red cells. A good correlation between the computer and a well-trained biologist was found after minor modifications of the computer's results. Image analysis should reduced the biologist-to-biologist variation and improve the identification and enumeration of the schistocytes.

Confidence Intervals↗
PubMed1992

(-)deprenyl and (-)parafluorodeprenyl-treatment prevents age-related pigment changes in the substantia nigra. A TV-image analysis of neuromelanin.

With the aid of TV-image analysis the number, the area and the density features of melanin granules in neurocytes of the substantia nigra in a group of 3-month-old naive male rats and in a 21-month-old group of male rats treated for 18 months with saline, (-)deprenyl and (-)parafluorodeprenyl, respectively, were determined. According to the Kolmogorov 2-sample test the two drug-treated groups do not differ significantly from each other in the number, total area and area of one pigment granule. In the aged saline-treated group the number of melanin granules decreased significantly and the area of the melanin granules increased in comparison to young controls as well as to the drug-treated groups.

Aging↗
PubMed1979

[Image analysis application at immuno-hematology (author's transl)].

Image analysis is an original method for the determination of blood groups and tissue types and for the serology of syphilis. Major advantages are the rapid and specific discrimination of biological particles present in agglutinated or free form, either fluorescent or stained. The reading of the tests is instantaneous and quantitative. The results are expressed immediately and stored with the use of computers. We have studied three micromethods, two for red blood cell grouping and irregular antibodies screening and one for the serology of syphilis, each one being adapted for an automatic system.

Histocompatibility Testing↗
PubMed1981

The development of a cervical smear preparation procedure from the BioPEPR image analysis system.

The preparation procedure for the BioPEPR automated image analysis system is described. Cervical cells are collected in a preservative solution and disaggregated by an automated syringing apparatus, giving about 50% single cells. After centrifugation, the preservative solution is discarded, 2% polyethyleneglycol in 50% ethanol (carbowax) is added, and the cell solution is automatically spread onto a glass slide, resulting in a cell density of about 50 cells/sq mm in the central area of the smear. Increasing cell density is shown to lead to a gradual linear decrease in single cells. All preparation steps have been critically evaluated, and the possibility of implementing this preparation procedure in a fully automated system is discussed.

Autoanalysis↗
PubMedDate not supplied

[Image analysis of naso-sinusal pathology with computerized tomography].

Imaging analysis has been applied to study typical cases of nasosinusal diseases, in an attempt to determine its capabilities for CT scan interpretations. Thus, polyps with sinusitis, cerebrospinal fluid occupying sinuses, cysts and bone tumors may be compared in order to obtain objective results that may help in the differential diagnosis in these conditions.

Diagnosis, Differential↗
PubMed1994

Image analysis of bromodeoxyuridine (BrdU) staining for measurement of S-phase in rat and mouse liver.

We developed a system for quantifying the numbers of bromodeoxyuridine (BrdU)-labeled hepatocyte nuclei in rat and mouse liver with an automated image analysis system. We began by developing a protocol for BrdU staining that would provide consistently intense staining to facilitate identification of both labeled and unlabeled nuclei by image analysis. Preliminary studies detected and characterized hepatocyte nuclei and differentiated them from non-hepatocyte nuclei using area and form factors. The parameters were selected to optimize discrimination between the two populations, selecting 90% of hepatocyte and 5% non-hepatocyte nuclei. Finally, we developed a program for automatic counting of BrdU-labeled hepatocyte and total hepatocyte nuclei. Results obtained from this method correlated well with data collected by a microscopist over a wide range of labeling indices. The automated system reduces interobserver variation and should minimize intraobserver error, as well as reducing the tedium of measuring labeling indices in the liver. Moreover, the techniques described should be applicable to other tissues.

Animals↗
PubMedDate not supplied

Suspension for intravenous injection: image analysis of scanning electron micrographs of particles to determine size and volume.

The purpose of this study is to determine the size and volume of large particles in a drug suspension by performing an image analysis of digital micrographs obtained by field emission, low voltage scanning electron microscopy (FE-LVSEM). The data obtained by this method are then used to select the appropriate imaginary component of the complete refractive index necessary for the software computation of particle size distribution measured using laser light diffraction. The application of FE-LVSEM involves four major elements: 1) the use of Anodisc inorganic aluminum oxide membrane filters for the image analysis of drug crystal particles > or = 350 nm having been isolated from suspension in order to determine the area, length, width, and other particle measurements; 2) the use of either a Thornley Everhart secondary electron detector or a MCP detector in the secondary electron mode directly above the specimen so as to produce a shadow-free digital image; 3) recording digital images as viewed normal to the crystal surface and at 45 degrees so as to image the edge of the crystal at a known angle; and 4) determination of drug particle volume from both views and the conversion of those volumes to an equivalent spherical volume.

Drug Stability↗
PubMed1995

The use of the digital cell image analysis of Feulgen-stained nuclei to detect apoptosis.

Cell death is an essential event in the functioning of multicellular organisms. It plays a role opposite to that of mitosis in the regulation of cell populations. In the present work, we describe an original methodology which permits the easy detection and count of apoptotic cells in a given tissue. This methodology is based on the digital cell image analysis of Feulgen-stained nuclei, which also permits the calculation of the proliferation index, i.e. the percentage of cells in the S phase of the cell cycle. This percentage of cells in the S phase is strongly related to the mitotic index. Our methodology, which involves the multivariate analysis of 14 morphonuclear parameters computed by means of the digitized cell image analysis of Feulgen-stained nuclei, was applied here to a well-known biological apoptosis model, namely glucocorticoid-treated rat thymocytes. The parameters that permitted the detection of apoptotic cells were the integrated optical density, a parameter that describes the nuclear DNA content, and the run length percentage and long run length parameters which are related to the pattern of chromatin condensation. This determination can be carried out on a relatively small number of cells.

Animals↗
PubMedDate not supplied

[Comparative studies of carcinomas of the human oral mucosa and xenografts in the nude mouse with the aid of computer-supported microscope image analysis].

The complete preservation of the original characteristics of donor tumors after xenografting into nude mice is more and more doubtful. With the aid of traditional biological methods and computer-assisted microscope image analysis the original human tumor tissues, including primary and recurrent tumors and metastases, are compared with nude mouse-grown xenografts. Squamous cell carcinomas of the oral mucosa served as original tumor tissues. Tumor takes were observed in 7 out of 12 cases. 5 transplantable lines could be established. Primaries, recurrent tumors and lymph node metastases are suitable for transplantations into nude mice and yield a comparable success rate. With one exception complete conformity between the original human tumor tissues and nude mouse-grown tumors could be found regarding the degree of differentiation and keratinizing tendency. As to the growth pattern of xenografts characterized by continuous shortening of growth periods, certain variations in the biological properties due to selection processes cannot be excluded. The results of microscope image analysis based on 35 different parameters suggest important differences within the developmental tendency of human tumor tissues and nude mouse-grown xenografts.

Animals↗
PubMedDate not supplied

Color image analysis in neuroanatomical research: application to senile plaque subtype quantification in Alzheimer's disease.

Many problems in neuroanatomy and neuropathology require the collection of large data sets and would benefit from a method that allows for rapid quantitative analysis to be carried out on a routine basis. An example is the quantification and subtype classification of the number of senile plaques in post-mortem Alzheimer's disease tissue. A method to reliably automate the analysis of plaques and their underlying subtypes would allow more rigorous and quantitative correlations to be investigated. Computer assisted image analysis of data typically utilizes gray scale images. These methods, however, are only applicable to quantification of objects labeled with a single marker. We sought to extend this type of analysis to double-labeled tissue sections so we could quantify dual labels separately based on their peroxidase color characteristics, analyze the resultant occurrence of overlap between the two labels, and classify senile plaques into discrete subtypes. We present a method for semi-automated color image analysis which allows one to identify separate labels based on histogram mapping of hue, saturation and value as well as apply overlapping feature detection algorithms. The technique is application driven, so that a trained observer can set threshold or object criteria and verify the desired results. These methods were able to yield total "amyloid load" and "dystrophic neurite load" values, generate plaque histograms based on total size, and subtype plaques into diffuse/primitive and neuritic/classical categories. By adjusting feature criteria, we were able to achieve promising agreement (Fisher's R to Z correlation of 0.94) between a human observer and the computer algorithm in the classification of plaque subtypes using three AD cases.

Algorithms↗
PubMed1990

Age estimation by IBAS image analysis of teeth.

The study of teeth to estimate age in a cadaver or in remains has become widely accepted in legal medicine, especially since the appearance of Gustafson's work in 1950. However, many studies published since that time have questioned the accuracy of these determinations. Computerized image analysis (IBAS) provides much more precise measurements of the parameters involved in these studies. An IBAS I (Kontron) image analysis system was used to study 19 different morphological parameters in midsagittal sections of a total of 173 upper and lower central incisors from as many live subjects. The data thus obtained provide useful information for determining the subject's age.

Adolescent↗
PubMedDate not supplied

Multiple synchronous colorectal carcinomas: a ploidy study by image analysis.

Cytometry could represent an ancillary technique to morphology in order to understand whether multiple synchronous colorectal carcinomas arose independently. Twenty-eight multiple synchronous tumours, assessed by means of a computerized image analysis system for DNA ploidy, were categorized as diploid (4) or not diploid (24). The ploidy classes were: DNA-diploid, DNA-tetraploid, and DNA-aneuploid. The DNA Index (DI) ranged from 0,90 to 2,66. The overall concordance rate for ploidy and DI class with synchronous tumours was 69% and 31%, respectively. The high concordance rate in ploidic categories suggests the metastatic origin of our multiple synchronous tumours. Moreover, they showed site, Dukes' classification, degree of differentiation, percentage of ploidy class, and DI distribution comparable to the single colorectal carcinomas. In conclusion, image analysis is a reliable technique to determine the independent clonality or the common origin of multiple colorectal tumours when the evaluation based on the simple histopathological criteria is not satisfactory.

Aged↗
PubMed1995

Morphometry of stallion spermatozoa by computer-assisted image analysis.

Metric measurements of stallion spermatozoal heads were determined for live, unfixed spermatozoa and for Feulgen-stained spermatozoa by videomicroscopy and computerized image analysis. Two ejaculates were collected from each of five stallions of normal fertility. Air-dried semen smears were Feulgen-stained, and live, unfixed spermatozoa were examined as wet-mount preparations. For Feulgen-stained spermatozoa, videoimages (x3850) were captured, and sperm heads were detected via image segmentation and particle analysis. For live, unfixed spermatozoa, phase contrast videoimages (x3850) were measured to determine width and length of the sperm head. For Feulgen-stained spermatozoa, there were significant effects (P < 0.001) of stallion and ejaculate on measured parameters of area, circumference, and the length and width of the sperm head. For live, unfixed spermatozoa, there were significant effects of stallion on length and width and of ejaculate on length of the sperm heads. There was a very poor correlation between length and width of sperm heads between Feulgen-stained and live, unfixed spermatozoa. Two indices of sperm shape (oval factor and aspect ratio) were also determined. Both aspect ratio and oval factor were significantly affected by stallion (P < 0.001); however, oval factor was not affected by ejaculate and therefore may represent a less variable determination of sperm head shape across stallions. Overall, length and width of stallion sperm heads were larger (P < 0.01) for live, unfixed spermatozoa than for Feulgen-stained spermatozoa (length: 6.3 +/- 0.4 vs 5.08 +/- 0.44; width: 3.08 +/- 0.34 vs 2.71 +/- 0.28 mum, respectively). Computerized image analysis may be useful as a means to objectively measure sperm head dimensions in the stallion and could be useful in future studies to determine associations with stallion fertility.

Journal Article↗
PubMedDate not supplied

The use of continuous monitoring and computer-assisted image analysis for the histochemical quantification of hexokinase activity.

The effect of endogenous 6-phosphogluconate dehydrogenase (6PGDH) on the histochemical quantification of low-Km hexokinase activity in rat submandibular salivary glands has been investigated using a Seescan Solitaire Plus image analysis system and a modified black and white Newvicon TV camera. Absorbance readings of neutral density filters were close to their known absorbance values. A significant correlation was found between the absorbance of Nitro BT reduction products within sections, with and without the use of a 588 nm interference filter. Furthermore, absorbance readings obtained from 8 microns-thick sections were 1.92 and 2.22 times greater than values obtained from 4 microns-thick sections using 'white' and 588 nm light respectively. The level of background illumination was not critical for absorbance measurements provided it was below the level that saturated the Newvicon camera and was not changed between background and image capture. The greatest variations in absorbance readings on tissue sections were associated with changes in zoom and objective combinations. Our studies indicate that this relatively low cost image analysis system can give reproducible absorbance readings from various structures defined in digitized, captured images of tissue sections. Results from continuous monitoring studies indicated that the high levels of 6PGDH activity in excretory ducts caused a 1.67-fold overestimation of hexokinase activity as assessed by absorbance readings performed throughout a 22-minute reaction period. By contrast, overestimation of hexokinase activity in salivary gland acini only became apparent after 8 min incubation with a 1.4-fold overestimation being seen at 22 min. This difference appears to reflect the relatively low hexokinase and 6PGDH activities present in acini compared with excretory ducts.

Animals↗
PubMed1993

Immunohistochemical determination of estrogen and progesterone receptor content in human breast cancer. Computer-assisted image analysis (QIC score) vs. subjective grading (IRS).

Immunohistochemistry of Estrogen Receptor (ER) and Progesterone Receptor (PR) has been performed in 687 cases of human breast cancer. The staining results have been compared by (1) computer-assisted image analysis (QIC Score) and (2) subjective grading of the cryostat sections ("German" IRS). Tumors without or with only weak ER or PR content may be distinguished by both methods from tumors with a high receptor content. The QIC Score values belonging to the intermediate IRS grades are distributed over a wide range, but no negative cases were found in these categories. It is concluded from our results that subjective grading of the slides is a simple, rapid and useful method for the determination of the tissue receptor content and must not be replaced by the expensive and time-consuming computer-assisted image analysis in daily practice.

Breast Neoplasms↗
PubMedDate not supplied

A modification of the staining technique of reticular fibres for image analysis of the cardiac collagen network.

INTRODUCTION: Silver stain of reticular fibres demonstrates the fine structure of the cardiac collagen network. However, nuclei are also stained with current techniques, a drawback that makes computer image analysis difficult. To solve this problem our study was designed to modify Gomori's method. Reactive concentrations and action times represent the core of that modification. Only stromal tissue is stained. The technique was tested for repeatability and reproducibility to assess the precision of the measurement procedure in the assessment of myocardial collagen in a consecutive series of myocardial samples from patients with and without heart muscle disease. In addition, we checked the reliability of the method by comparing our results with the point-counting method (PCM). METHODS: The right ventricular myocardium was fixed in 10% buffered formaldehyde and routinely processed. Paraffin sections (4 microm) were stained with several modifications of Gomori's method. Variable concentrations of potassium permanganate, silver solutions and different oxidation times were tried. A field from each sample was digitized. Additionally, the technique was tested for repeatability and reproducibility. RESULTS: We obtained absence of background and nuclear staining together with a highly contrasted image of the collagen network with 3 min oxidation with 2% potassium permanganate and a silver concentration of 1% during 5 min. In this way, it was very easy to perform acquisition, thresholding and area measurement without any further manual processing of the image. CONCLUSIONS: This technique appears very helpful for the quantitative study of the cardiac collagen network by means of computerized image analysis systems.

Adolescent↗
PubMed1987

Sources of variance in the objective documentation of human cataractous change with Topcon SL-45 and Neitz-CTR retroillumination photography and computerized image analysis.

In the application of Topcon SL-45 Scheimpflug slit and Neitz CTR retroillumination photography to in vivo documentation of cataractous change, several sources of variance affect the results of each technique. We have measured the between-person, between-photographer, between-focal plane (Neitz), between-photo session, replicate photograph (Topcon), between-image analyst and replicate image analysis variances encountered in in vivo documentation of human cataracts with the Topcon SL-45 and Neitz-CTR cameras and our system of computerized image analysis.

Adult↗