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Results for “ULTRACENTRIFUGATION”

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At least 811 records · Page 45Linked to original sources
PubMed1979

Evaluation of a class III biological safety cabinet for enclosure of an ultracentrifuge.

An evaluation of a special safety cabinet housing a high-speed centrifuge was made. The cabinet enclosed both the top access port and the drive and pumping machinery of the centrifuge. A titanium rotor was loaded with tubes containing a bacterial culture, weakened, and driven until rotor rupture occurred. There were several bent and broken components in the centrifuge, and bacteria leaked from the vacuum chamber. Although the forces were sufficient to displace the cabinet, none of the test bacteria were found outside the cabinet.

Aerosols↗
PubMed1984

Human rotavirus detection by counterimmunoelectrophoresis versus enzyme immunoassay and electron microscopy after direct ultracentrifugation.

The sensitivity of the counterimmunoelectrophoresis test with NCDV, Wa, and SA-11 rotavirus antisera was 60, 60, and 67%, respectively. The counterimmunoelectrophoresis specificity was greater than 99%, but the low sensitivity is a limiting feature of this test as a first-line immunodiagnostic test for rotavirus detection.

Counterimmunoelectrophoresis↗
PubMed1976

Limitations of ultracentrifugation and in vivo dialysis as methods of stool analysis.

Extracellular stool fluid has been sampled by in vivo dialysis, in vitro dialysis, and high speed centrifugation. Detailed comparisons of amino acids and electrolytes have been made between each fluid; normal values and significant differences are summarized. Evidence is provided which suggests that there is release of intracellular contents during normal handling of stool in the laboratory. The amino acids which display the greatest differences between centrifugate and dialysate from given stool specimens are those which are liberated by ultrasonication or aerobic incubation of stool. Factors affecting the chemical stability of stool are discussed. In most circumstances in vivo dialysis would seem to provide the closest approximation to extracellular fluid, providing the faeces remain in the descending colon or rectum for at least two hours.

Air↗