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Clinical use of refrigerated flexor tendon allografts to replace a silicone rubber rod.

Thirty-five patients with grades II through V flexor tendon damage in 42 fingers were treated first with a silicone rubber rod prosthesis and later with a flexor tendon allograft. The final results were evaluated by recording the distance each finger lacked in flexing to the distal palmar crease and by recording the total active motion of each finger. By the first measurement, 63% had good flexion, 21% fair, and 16% poor; and 8% had good total active motion, 71% fair, and 21% poor. An allograft can be used successfully in a two-stage reconstruction of flexor tendons, but based on other reports, an autograft may provide somewhat better results.

Adolescent↗

Effect of different treatments on the evolution of polyamines during refrigerated storage of eggplants.

The applicability of a thermal treatment was compared with modified-atmosphere (MA) storage in relation to chilling injury (CI) and polyamines evolution in eggplants. Fruits underwent physiological disorders at 3 degrees C, evidenced by the appearance of surface injuries at the third day of storage, and, after moving the fruits to 20 degrees C, by increased respiratory activity and more intense ethylene production. Storage of fruits in sealed low-density polyethylene bags and a previous treatment with heated air (1 h at 35 degrees C) were both effective in retarding chilling injury, though the former was better. Two free polyamines were found in cv. Black Nite: putrescine, in greater proportion, and spermidine. Putrescine increased in control (untreated) fruits stored at 3 degrees C in parallel with the external appearance of chilling injury, whereas this increase was either not exhibited or retarded in treated or MA stored fruits. Spermidine did not change in control fruits at 3 degrees C, remaining almost constant over the whole storage period, whereas in heat- and MAP-treated fruits spermidine levels exhibited a decrease.

Air↗

Health-promoting compounds in broccoli as influenced by refrigerated transport and retail sale period.

Total aliphatic and indole glucosinolates, phenolic compounds (flavonoids and hydroxycinnamoyl derivatives), and vitamin C contents were evaluated in freshly harvested broccoli (Brassica oleracea L., var. italica, cv. Marathon) inflorescences. These were film-wrapped and stored for 7 days at 1 degrees C to simulate a maximum period of commercial transport and distribution. After cold storage, inflorescences were kept for 3 days at 15 degrees C to simulate a retail sale period. For wrapping, low-density polyethylene (LDPE) of 11 microm thickness was used. Gas composition was about 17% O(2) and 2% CO(2) during cold storage and about 16% O(2) and 3-4% CO(2) during shelf life within packages. The predominant glucosinolates were 4-methylsulfinylbutyl-glucosinolate (glucoraphanin), 3-indolylmethyl-glucosinolate (glucobrassicin), and 1-methoxy-3-indolylmethyl-glucosinolate (neoglucobrassicin). The predominant hydroxycinnamoyl derivatives were identified as 1,2,2'-trisinapoylgentiobiose, 1,2-diferuloylgentiobiose, 1,2'-disinapoyl-2-feruloylgentiobiose, and 3-O-caffeoyl-quinic (neochlorogenic acid). Results showed major losses at the end of both periods, in comparison with broccoli at harvest. Thus, the respective losses, at the end of cold storage and retail periods, were 71-80% of total glucosinolates, 62-59% of total flavonoids, 51-44% of sinapic acid derivatives, and 73-74% caffeoyl-quinic acid derivatives. Slight differences in all compound concentrations between storage and retail sale periods were detected. Distribution and retail periods had minimal effects on vitamin C. Weight loss was monitored at the end of both periods.

Ascorbic Acid↗

Biochemical, Microbiological, and Sensory Changes of Sea Bass (Lateolabrax japonicus) under Partial Freezing and Refrigerated Storage.

Packages of fresh sea bass (Lateolabrax japonicus) were stored under temperatures ranging from -3 to 10 degrees C. Changes in K value, volatile basic nitrogen, aerobic plate count, and sensory acceptability were monitored. The shelf life of stored sea bass at 5 degrees C was 3 days. It was extended to about 2 weeks at 0 degrees C. Partial freezing storage at -3 degrees C increased the shelf life to >4 weeks. Ice and partial freezing temperatures increased the shelf life by lengthening the lag phase of bacterial growth and retarding the nucleotide breakdown rate. A maximum K value of 50% was appropriate for sea bass shelf life determination. In contrast, a maximum microbial population of 3 x 10(6) CFU/g of fish muscle was a good shelf life indicator only at storage temperatures >/=0 degrees C. A rapid rise in volatile basic nitrogen could serve as an indication of substantial microbial spoilage.

Journal Article↗

The biochemical effects of CPDA-2-drawn red blood cells of delayed refrigeration prior to component preparation.

The effects of an 8-hour hold at 22 degrees C prior to component preparation were evaluated in a split-bag study using nine units of blood preserved in citrate-phosphate-dextrose-adenine (CPDA-2). Each unit was divided in half, platelet-rich plasma removed at 0 or 8 hours, respectively, and the half units of red blood cells stored at 4 degrees C for 42 days. The only red blood cell metabolic differences seen in the bags held 8 hours (compared to those not held) were a 21 percent rise in adenosine triphosphate, which was not significant after 14 days of storage, and a 33 percent loss of 2,3-diphosphoglycerate which resulted in a loss curve similar to that seen with acid-citrate-dextrose blood. The logistic advantages seem to warrant an 8-hour holding period for red blood cells drawn in CPDA-2.

Adenine↗

Effect of delayed refrigeration on plasma factors in whole blood collected in CPDA-2.

Extension of the time within which whole blood may be separated into components offers logistic advantages for the operation of remote mobile drawing teams. We evaluated the effect of an 8-hour hold of whole blood at room temperature before preparation of components. Plasma coagulation activity and opsonic factor content were studied in 14 units drawn into the anticoagulant-preservative solution citrate-phosphate-dextrose-adenine (CPDA-2). At the time of collection, an additional 7-ml aliquot was drawn into 1 ml of CPDA-2, the plasma separated and frozen immediately. Components were prepared from whole blood units allowed to rest undisturbed at 22 +/- 1 degrees C for 8 hours. After 8 hours, a significant decrement of about 10 percent was found in the concentration of fibrinogen, plasminogen, fibronectin, and activity of Factor V. Factor VIII activities (VIIIAHF and VIIIAGN) were not significantly different after 8 hours. Our results indicate that room temperature storage for 8 hours before component processing has minimal effects on potentially labile plasma protein factors using CPDA-2 anticoagulant-preservative solution.

Adenine↗