[Stimulation of cortisone and radioresistant mouse lymphoid cells in vitro by phytohaemagglutinin-P (PHA), pokeweed mitogen (PWM) and concanavalin A (Con A)].
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Animals↗
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In-vitro inhibition by unsaturated fatty acids of the PPD- and PHA-induced lymphocyte response.
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Aniline Compounds↗
Isolation of the lymphoagglutinating fractions of phytohaemagglutinin (PHA) from phaseolus vulgaris and characterization of their biological properties.
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Animals↗
Inhibition of the cytoaggressive effect of phytohaemagglutinin (PHA)-stimulated human lymphocytes by E. coli L-asparaginase (EC-2 A-SE).
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Asparaginase↗
Studies on the nature of the PHA receptor.
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Animals↗
PHA -blastogenesis in relationship to the cell-type and source in acute leukemia.
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Bone Marrow↗
The acridine-orange fluorescent method in PHA-stimulated lymphocytes in culture.
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Acridines↗
[Lymphocyte culture in Hodgkin's disease: indications of 2 lymphocyte populations with different reactions to phytohemagglutinis (PHA)].
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Culture Techniques↗
An in-vitro quantitative study of phytohaemagglutinin (PHA) induced transformation of lymphocytes from premature newborn infants, from older premature infants, and from full-term newborn infants.
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Culture Techniques↗
[Differential reaction of lymphocytes to phytohemagglutinin (PHA) in liver diseases].
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Adolescent↗
Cell cycle analysis and properties of two sub-populations in PHA responding lymphocytes. A comparison of 21-trisomic and normal cells.
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Cell Cycle↗
Kinetics of division in PHA-stimulated pig lymphocytes.
Our results indicate that pig lymphocytes in culture complete their 1st division at 24 h. At 36 h there are 9% of cells in 2nd division. 3rd mitosis appears at 48 h; and at 72 h there are cells engaged in the 4th division.
Animals↗
Induction of micronuclei in PHA-stimulated human lymphocyte cultures by therapeutic radiation.
Micronuclei frequency and percent of chromosome breaks increases significantly in adults whose thymus glands were irradiated in infancy and after irradiation of cancer patients.
Adult↗
Selective effects of PHA on rat brush border hydrolases along the crypt-villus axis.
The mechanism of the toxicity of lectin from Phaseolus vulgaris seeds has been investigated on rat enterocytes. Cell isolation procedures showed a selectivity in the loss of brush border hydrolases; this indicated that the microvilli blebbing was not the only mechanism of action of lectins on rat enterocytes.
Animals↗
Effect of supernatants from PHA-stimulated and non-stimulated lymphocyte cultures on thromboxane B2 release by polymorphonuclear leukocytes in vitro.
The present study involves the determination of the effect of supernatants obtained from mitogen-induced lymphocytes of human tonsils and containing lymphotoxins on human peripheral blood polymorphonuclear leukocytes (PMNs). PMNs treated with supernatants from phytohemagglutinin-stimulated lymphocytes were found to increase the release of thromboxane B2, compared to normal control levels. This increase was blocked by the preincubation of PMNs with indomethacin. The production or increase in production of thromboxanes may be induced by lymphotoxins since these substances have been reported to activate phospholipase A2 in target cell membranes, which may result in release of the thromboxane precursor, arachidonic acid.
Humans↗
The effect of L-asparaginase on the synthesis and processing of ribosomal precursor RNA in PHA-stimulated lymphocytes.
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Asparaginase↗
Cytophotometric studies of the course of the S phase in PHA stimulated lymphocytes.
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Cells, Cultured↗
Small nuclear RNAs synthesis in PHA-stimulated and nonstimulated human peripheral blood lymphocytes.
Five fractions of small nuclear RNAs (snRNAs) were identified in nuclei of human peripheral blood lymphocytes. They have been designated as: H, G', D, C and A (nomenclature according to Weinberg) in order to decreasing electrophoretic mobility. Their synthesis was observed from the first day of treatment with mitogen and was continued with different intensity during four days of culture. In nonstimulated lymphocytes exclusively A and G' fractions were detected but no (14C)-uridine incorporation was observed.
Cells, Cultured↗