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At least 811 records · Page 45Linked to original sources
PubMed2003

Forensic applications of chemical imaging: latent fingerprint detection using visible absorption and luminescence.

Chemical imaging technology is a rapid examination technique that combines molecular spectroscopy and digital imaging, providing information on morphology, composition, structure, and concentration of a material. Among many other applications, chemical imaging offers an array of novel analytical testing methods, which limits sample preparation and provides high-quality imaging data essential in the detection of latent fingerprints. Luminescence chemical imaging and visible absorbance chemical imaging have been successfully applied to ninhydrin, DFO, cyanoacrylate, and luminescent dye-treated latent fingerprints, demonstrating the potential of this technology to aid forensic investigations. In addition, visible absorption chemical imaging has been applied successfully to visualize untreated latent fingerprints.

Aza Compounds↗
PubMedDate not supplied

[Genetic fingerprinting (PFGE) of bacterial isolates for their molecular epidemiology].

The determination of fragment patterns of bacterial genomic DNA after digestion with long-range-cutting restriction enzymes and their separation in pulsed-field-gelelectrophoresis is currently regarded as reliable fingerprinting tool for surveillance and identification of infectious sources and routes of bacterial pathogens. The PFGE based molecular fingerprinting has been applied and used for a broad range of bacterial species. As a prerequisite for its application in national and international network studies (German PulseNet, PulseNet USA, Salmgene) a common protocol for rapid production (1-2 days) of standardised and electronically readable fragment patterns has been developed. National and international outbreaks, diffuse outbreaks and long term surveillance of the ups and downs of pathogens have been successfully carried out by means of PFGE studies. The international outbreak of Salmonella enterica serovar Oranienburg (2001) due to contaminated chocolate is described here underlining the usefulness of PFGE as genetic fingerprinting for epidemiological purposes.

DNA, Bacterial↗
PubMed2003

[Study on the fingerprints of Achyranthes bidentata Bl. by HPLC/UV/MS].

OBJECTIVE: To establish the fingerprints of Achyranthes bidentata Bl. by HPLC/UV/MS. METHOD: Separation was performed on Aglient Zorbax SB-C18 column. Gradient elute was performed by the mobile phase consisting of methanol and 0.1% formic acid-water with the flow rate of 1.0 ml/min. RESULT: Perfect fingerprints were obtained which can be used for the evaluation of Achyranthes bidentata Bl. Four common peaks were confirmed in fingerprints. Three compounds were elucidated as 5-hydroxymethyl furaldehyde, ecdysterone, inokosterone by HPLC/MS. CONCLUSION: The method can be applied to the quality control of Achyranthes bidentata Bl.

Achyranthes↗
PubMed1992

A rapid gas chromatographic method for the fingerprinting of illicit cocaine samples.

A gas chromatographic (GC) fingerprint method, based on the presence or absence of six congeners, was developed for illicit cocaine samples. The fingerprint utilizes the relative abundances of these congeners towards each other, disregarding cocaine as the main constituent, and can be expressed numerically or graphically in the form of pictograms for rapid visual comparison. The method can be applied directly to a solution of the sample in chloroform, without previous workup procedures. More than 70 unrelated samples were analyzed and a great variation was observed in the parameter composition. On the other hand, a remarkable similarity could be seen between related samples. The GC fingerprint method may be considered an important contribution for sample comparison, as is exemplified by a subdivision of the analyzed samples in different categories, based on the number and types of congeners found.

Bridged Bicyclo Compounds↗
PubMed2003

[Identification on genuine and pseudo shayuanzi collected from different place by HPLC fingerprint].

OBJECTIVE: To establish the identification method for genuine and pseudo Shayuanzi by HPLC fingerprint. METHOD: The flavone, a kind of active composition in genuine (Astragalus complanatus) and analysed species was analysed by RP-HPLC. RESULT: The fingerprints of genuine Shayuanzi collected from different places showed the similar characteristics, but they were different from those of pseudo species A. adsuragen and A. sinicus. CONCLUSION: The HPLC fingerprint can be an identifing method for Shayuanzi.

Astragalus Plant↗
PubMed2004

[Pattern recognition applied to the fingerprint of traditional Chinese medicine characterized by two-dimension information data].

AIM: To establish the two-dimension information fingerprints chromatograph of traditional Chinese medicine and investigate via pattern recognition. METHODS: Hierarchical cluster was applied to fingerprints described by one-dimension information data and by two-dimension information data respectively and corresponding results was compared. RESULTS: The gross classes, in which the samples denoted by two-dimension information data, classified by all kinds of hierarchical cluster methods had less differences and more robustness than the methods containing the samples expressed by one-dimension information data. The classes to which the unknown samples would be belonged were determined by hierarchical cluster analysis and artificial neural network (ANN), and the same results were obtained. CONCLUSION: The fingerprints characterized by two-dimension information data could provide more overall and special information as compared with the methods indicated by one-dimension information data.

Chromatography, High Pressure Liquid↗
PubMed2004

[Establishment of chromatographic fingerprint and quality assessment of Carthamus tinctorius L. by high performance liquid chromatography].

AIM: To establish chromatographic fingerprint of Carthamus tinctorius L. by RP-HPLC in order to control the quality of Carthamus tinctorius L. METHODS: The gradient elution mode was applied in chromatographic separation, and data were analysed by "Computer Aided Similarity Evaluation" software to compare the quality of Carthamus tinctorius L. samples from different habitats. RESULTS: Samples from different habitats were of high similarity, though a few samples showed evident difference in fingerprint graphics. CONCLUSION: The RP-HPLC fingerprint method is repeatable, feasible in analysis of Carthamus tinctorius L. and can be used in quality assessment of Carthamus tinctorius L. Chemical components in Carthamus tinctorius L. samples from various habitats are similar, and their ratios between each other are stable.

Carthamus tinctorius↗
PubMed2005

[Studies on fingerprint spectrum of Radix Sophorae Tonkinensis from Guangxi].

OBJECTIVE: To set up the fingerprint spectrum of Radix Sophorae Tonkinensis from Guangxi province. METHODS: HPLC with UV detector was used to analyze matrine and oxymatrine the patterns of Radix Sophorae Tonkinensis from Guangxi province. RESULTS: The fingerprint spectrum of Radix Sophorae Tonkinensis from Guangxi province was set up. Furthermore, the fingerprint spectrum showed an excellent correlation, and all the parameters of the main co-peaks met the requirements for State Drug Administration's technical regulations on the herb. CONCLUSION: The studies are useful for the standard planting and the quality control of Radix Sophorae Tonkinensis from Guangxi province.

Alkaloids↗
PubMed2005

[Study on fingerprint chromatograms of water-soluble constituents of Salvia miltiorrhiza Bge. by high performance liquid chromatography].

The fingerprint chromatograms were established for the quality evaluation of Salvia miltiorrhiza Bge. by high performance liquid chromatography. The analysis was performed on a Zorbax SB-C18 column (5 microm, 4.6 mm i.d. x 250 mm) with acetonitrile-water (containing 0.4% (v/v) acetic acid ) (the volume fraction of acetonitrile from 0 to 40% within 70 min) as mobile phase at a flow rate of 1.0 mL/min, and at a column temperature of 25 degrees C. The detection wavelength was 254 nm. The relative standard deviations of relative retention values and relative peak areas were less than 3%. The mutual fingerprint of the extract of Salvia miltiorrhiza Bge. collected from Danshen Base in Tongren City of Guizhou Province was established by using the similarity calculation software of Chinese herbal fingerprint. The method is reliable and can be helpful in effectively controlling the quality of Salvia miltiorrhiza Bge.

Chromatography, High Pressure Liquid↗
PubMed2005

[Study of HPLC chromatographic fingerprint of Citrus reticulata Blanco cv. Dahongan].

OBJECTIVE: To establish HPLC chromatographic fingerprint of Citrus reticulata Blanco cv. Dahongan. METHODS: The experimental condition of the RP-HPLC method was as follows: Angilent Zorbax C18 column (4.6 mm x 250 mm, 5 microm), with linear gradient elution using (A) methanol and (B) water; The flow rate was 1.0 ml/min, and the injected volume was 20 microl, detected wavelength was 283 nm. RESULTS: Under the qualitative conditions we determined different samples of Citrus reticulata Blanco cv. Dahongan. There were 11 common peaks in chromatographic. CONCLUSIONS: According to analyze and contrast the relative retention values and relative peak area in chromatographic fingerprint, we establish HPLC chromatographic fingerprint of Citrus reticulata Blanco cv. Dahongan to provide a scientific basement for the quality evaluation of it.

Chromatography, High Pressure Liquid↗
PubMed2005

[HPLC fingerprint of Fritillaria hupehensis].

AIM: To establish a fingerprint analysis method of Fritillaria hupehensis. METHODS: fingerprint was performed by HPLC-ELSD. Hypersil ODS column was used; the mobile phase was composed of methanol (with 0.05% triethylamine) and water with gradient elution; flow rate was 1.0 mL x min(-1); recording time was 60 min; drift tube temperature was 75 degrees C; gas flow rate was 1.9 L x min(-1). RESULTS: HPLC fingerprint of Fritillaria hupehensis was obtained. CONCLUSION: A reliable method was provided for controlling the quality of Fritillaria hupehensis.

Alkaloids↗
PubMed2005

[Study on HPLC fingerprint and quality control of Propolis].

OBJECTIVE: To study on HPLC fingerprint of Propolis and to control it's quality. METHOD: The chromatographic fingerprints of seven samples from different producing areas were determined by RP-HPLC. RESULT: The chromatograms of Propolis from different producing areas were very similar. CONCLUSION: The quality of Propolis can be controlled by determination the HPLC fingerprint.

Chromatography, High Pressure Liquid↗
PubMed2005

Genipin, a novel fingerprint reagent with colorimetric and fluorogenic activity, part II: optimization, scope and limitations.

Genipin, a hydrolytic product of geniposide extracted from gardenia fruit, was thoroughly studied as a potential fingerprint reagent, and optimal conditions for fingerprint development have been determined. Latent fingerprints on paper items that have been treated with a non-ink running formulation containing 0.17% of the reagent, showed up as both colored and fluorescent images. On brown wrapping paper and on papers with highly luminescent backgrounds, genipin developed more visible and clearer prints than did classical reagents such as ninhydrin or DFO. Another potential advantage of genipin is that it is totally harmless and an environmentally friendly reagent.

Aza Compounds↗
PubMed2006

Circular fingerprints: flexible molecular descriptors with applications from physical chemistry to ADME.

Circular fingerprints -- the representation of molecular structures by atom neighborhoods -- have been applied to a wide range of applications, such as similarity searching and the prediction of absorption, distribution, metabolism, excretion and toxicity properties. In recent years there has been a surge in applications resulting from the superior performance of circular fingerprints in comparative studies. This feature examines the nature of circular fingerprints as well as their applications, including virtual screening, metabolism prediction and the estimation of pK((a)) constants.

Algorithms↗
PubMed1991

Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting.

Viral RNA oligonucleotide fingerprinting was used to discriminate 3 cytopathic vaccine bovine viral diarrhea viruses (BVDV) grown in medium supplemented with serum contaminated with noncytopathic BVDV from the same 3 viruses grown in cell culture free of BVDV. Oligonucleotide fingerprinting also effectively discriminated between reference Singer BVDV, NADL BVDV, and New York-1 BVDV grown in BVDV-free noncontaminated or BVDV-contaminated cell cultures. Oligonucleotide fingerprint mapping of viral RNA maybe used to determine the purity of virus stocks, as well as that of BVDV vaccines.

Animals↗
PubMed2006

[Identification on Spora Lygodii by X-ray diffraction fingerprint spectra method].

OBJECTIVE: To set up a new identification and analysis method of Spora Lygodii. METHOD: To get the extracts of Spora Lygodii using methanol, chloroform and petroleum ether as solvent and the extracts were identified, analyzed by X-ray diffraction Fourier fingerprint spectra. RESULTS: Experiments and analysis were carried out on three samples. The standard X-ray diffraction Fourier fingerprint spectra and characteristic diffraction peaks were obtained. There were some differences among the spectra of the extracts, but the characteristic diffraction peaks were obvious. CONCLUSION: The experimental result indicated that X-ray diffraction Fourier fingerprint spectra can be used to identify and analyze the Chinese traditional herb Lygodium japonicum (Thunb.) Sw.

Ferns↗
PubMed2006

[The effects of different clinicopathologic variables on serum protein fingerprint in hepatocellular carcinoma patients].

OBJECTIVE: To investigate the effects of different clinicopathologic variables on serum protein fingerprint in hepatocellular carcinoma (HCC) patients. METHODS: Serum samples were collected from 112 HCC patients, Special serum protein or peptide spectra was determined by surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) measurement after treating the sample onto weak cation exchange (WCX2) protein chip for each case. The serum protein profiles were compared by BioMarker Wizard Software among the patients stratified according to gender, AFP, presence of portal vein tumor thrombus (PVTT), tumor size, tumor number, presence of cirrhosis, respectively. RESULTS: According to serum protein fingerprints of 112 HCC patients, a total of 100 protein peaks were identified at the m/z value ranging from 1100 to 30,000. (1) Sixteen significant differential proteins were found between the groups of HCC with single tumor and those with multiple tumors (P < 0.01). (2) Only one significant differential protein was found between the groups of HCC with tumor size > 3 cm and those with tumor size 5 cm and those with tumor size 10 cm and those with tumor size <or= 10 cm (P < 0.01). (3) Sixteen significant differential proteins were found between the groups of macroscopic portal vein tumor thrombus (Ma-PVTT) and those without PVTT (N-PVTT); Only 2 significant differential protein were found between the groups of microscopic portal vein tumor thrombus (Mi-PVTT) and N-PVTT (P < 0.01); eight significant differential proteins were found between the groups of Ma-PVTT and Mi-PVTT (P < 0.01). (4) No significant differential protein was found when patients stratified according to gender, presence of cirrhosis and AFP. CONCLUSIONS: PVTT, tumor number and tumor size had significant effects on serum protein fingerprint, while no significant effect on serum protein from gender, presence of cirrhosis and AFP. The most profound impact on the serum protein was attained when cutoff was chosen to be presence of Ma-PVTT compared to less effect from Mi-PVTT and 5cm for tumor size.

Adult↗
PubMed2006

[Study on the HPLC fingerprints of Styela plicata].

OBJECTIVE: To establish the HPLC fingerprints of Styela plicata. METHOD: The HPLC assay was used to compare fingerprints of Styela plicata from different location. RESULTS: Experiment and analysis were carried on six samples, the standard HPLC fingerprint pattern method and 13 characteristic diffraction peaks of Styela plicata were obtained. CONCLUSION: This method is reliable and convenient for the identification and quality control of Styela plicata.

Animals↗