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Stereoscan studies of rediae, cercariae, cysts, excysted metacercariae and migratory stages of Fasciola hepatica.

The external surface of the redial body of Fasciola hepatica is provided with microvillus-like projections or short lamellae, and short cilium-like structures are common anteriorly. The anterior part of the cercarial body possesses a pattern of regularly arranged small depressions each containing a spine. Both long and short cilium-like structures occur anteriorly. The tail is spineless and provided with dorsolateral folds. The outer cyst wall is formed by granules secreted from the tegument all over the body apart from the ventral sucker. Most granules transform into fibrillae which form the thick outer spongy layer. The precursor of the inner cyst wall is at the beginning closely attached to the metacercarial surface, but later the membrane-like cyst wall extends, and when fully formed the metacercaria lies free in the flattened circular inner cyst. The ventral plug is formed by the ventral sucker. The tegument of newly excysted metacercariae is provided with simple pointed spines, but later during migration in the mouse the spines become flattened and multipointed. Very young migratory stages may be attached with host cells.

Animals↗

A histochemical study of the rediae and cercariae of Fasciola hepatica.

Histochemical studies have revealed hydrolytic enzyme activity in the redial tegument and caecal epithelium of Fasciola hepatica. A number of the hydrolases are known to be lysosomal marker enzymes and it is thought that they may be involved in intracellular digestion. The larval stages have also been shown to be reactive for succinate dehydrogenase and phosphorylase, but not for glucose-6-phosphatase.

Acid Phosphatase↗

Chaetogaster limnaei K von Baer 1872 on Lymnaea tomentosa: ingestion of Fasciola hepatica cercariae.

The fresh water mollusc, Lymnaea tomentosa, the intermediate host of the liver fluke is heavily infested under natural conditions with ectocommensal annelid, Chaetogaster limnaei. These annelids which destroy the larval stages of Fasciola hepatica have been observed in the laboratory. The association of C. limnaei with the snail intermediate host is of value in exploiting a control measure against economically important liver fluke disease.

Animals↗