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[Geobotanical studies on the island of Tanegashima (affinity of world property island Yakushima)].

A list was drawn up of wild plants growing on Tanegashiama island that were identified in our field work, and the list was compared with the flora of the rest of Japan and the flora of Taiwan. There were 166 families and 1,218 species consisting of 23 families and 159 species of Pteridophyta, 4 families and 7 species of Gymnosperma, 113 families and 700 species of the dicotyledous Angiosperma, and 26 families and 353 species of monocotyledous Angiosperma. There are 229 families and 5,500 species of plants in Japan, 196 families and 3,019 species in Kyushu, and 228 families and 3,477 species in Taiwan. There are 11 species of endemic plants on Tanegashima and Yakushima, and the best known of them is Pinus armandii Francht. var. amamiana Hatsushima. There are 181 species of flora of flora limited to the northern element, including several important medicinal plants, such as Akebia quinata Decaisne and Zanthoxylum piperitum DC. The 69 species of flora limited to the southern element include several important tropical plants, such as Messerschmidia argentea Johnston and Clerodendrum inerme Gaertn. Most of these plants are distributed on both island, but some of are distributed only Tanegashima. We concluded that one of the temperate borderlines of Japanese flora in the temperate zone is the islands of Tokara. The flora of Tanegashima and Yakushima are having a closely affinity of plant species and having the rich plant species.

Botany↗

[Patterns of spiral phyllotaxy].

The author investigates theoretical variants of spiral phyllotaxy using structural coefficient--the distance between consequent members in genetic spiral, expressed in parts of circle. The meanings of structural coefficient in which parastichies change their isomerism are determined as critical points. The author describes the overlapping of parastichies of lower lane by higher lane parastichies and presents the methods of overlapping determination. Parastichies with minimal distance between members in lane are clearly determined on shoots. Methods and formulas for calculation of structural coefficient using the number of members in parastichy circle are presented. Orthostichy is considered as a special case of parastichy location. Orthostichies are equal to parastichies in maximum critical points.

Botany↗

Micropropagation of sweet orange, Citrus sinensis Osbeck. for the development of nucellar seedlings.

Protocol for micropropagation of elite plants of sweet orange (Citrus sinensis) through nucellar embryo culture has been standardized. Three to four nucellar embryos and a zygotic embryo could be excised from a single mature seed and successfully generated as healthy plants in basal MS medium. MS medium supplemented with NAA (1 mg/L) or 2, 4.D (1 mg/L) promoted callus development in both nucellar and zygotic embryos. GA3 (1 mg/L) enriched medium induced plantlets initiation but their growth was very poor. No significant differences were observed between initial growth patterns of nucellar and zygotic seedlings developing from the same ovule. Five to six shoots were obtained from collar region of both category of embryos in MS medium supplemented with BAP (1 mg/L) within 60 days of inoculation. The number of plantlets were almost doubled after their transfer in the same medium and culture for another 30 days. Higher doses of BAP resulted in initiation of callus directly from the embryos. The regenerated shoots (2-3 cm) could be rooted in MS medium supplemented with either only NAA (0.75 mg/L) or NAA (0.50 mg/L) and IBA (2.0 mg/L). A number of plantlets could be obtained from a nucellar embryo grown shoot within a limited time period.

Botany↗

In vitro multiplication of Nothapodites foetida (Wight.) Sleumer through seedling explant cultures.

In vitro multiplication of Nothapodites foetida (Wight.) Sleumer was achieved using axenic seedling explant cultures. Isolated nodes (1.0-1.2 cm) and shoot tips (1.0-1.5 cm) cultured in Murashige and Skoog's agar medium containing varying concentrations of TDZ, BA and combinations of 2iP and GA3. Single shoot (0.8-1.2 cm) was regenerated in each culture after 6 weeks. Axillary shoots were then excised and recultured for 8 weeks in medium containing TDZ (0.05 mgL-1) which formed shoots (about 4 in no.; 2 cm) from the basal node. Axillary branches (2) which formed on 60% of these shoots after 10-12 weeks of culture were separated and recultured in the same medium for 8 weeks. Three shoots (0.8-1.0 cm) per culture were regenerated. Shoots of 0.8-1.8 cm length were subcultured on a low cytokinin (0.01 mgL-1 TDZ) regime to induce shoot elongation (2.0-3.5 cm) in 4 weeks. Shoot cuttings were rooted (60%) in the medium containing IBA (1.5 mgL-1). Rooted plantlets established in pots (90%) after hardening resumed normal growth in 3 months.

Botany↗

[A little known great botanist of indochinese flora: Paul Louis Simond].

Alongside his brilliant scientific and administrative career, Paul Louis Simond, a famous colonial doctor, was also an observer of nature and excellent botanist. Following in the tradition begun two centuries earlier by medical doctors and pharmacists in the marine and army health services, Paul Louis Simond showed particularly interest in the family of Indichenese orchids during the third visit he made to the country in 1914-1917, while he was inspector of the health service. There remain a splendid collection of 226 watercolours depicting the different species of orchids which he had painted by a local artist on live model he was cultivating himself or had had sent to him from all over Indochina both officially and privately. Most of the plates he determined himself, accompanying them by a thorough and exact hand-written description of the floral organs allowing their identification. His knowledge in the area was worthy of a professional and he contributed largely to the botanical description of a region which is still far from being completed. Whilst the plates in the collection are of unequal scientific value (sometimes they are unfinished or without a detailed floral description, or they are copies of already existing plates), the greater part (160) constitutes a considerable original contribution to the knowledge of Indochinese orchidological flora (21 types). The entire collection was bequeathed in 1947 to the laboratoire of Phanérogamie of the Muséum National d'Histoire naturelle, where it can be consulted by scholars.

Botany↗

[Animal nutrition in the dietetics of the 18th Century with "Materiae Medicae" by H.J.N. Crantz as an example].

H.J.N. Crantz (1722-1797), a physician and botanist born in the Duchy of Luxembourg and practising in Vienna under the Empress Maria Theresia during the 18th century, deals in his "materiae medicae et chirurgicae", written in Latin language, also with animal foodstuff. Animals from various species are commented on the utility of their meat and products to maintain health or to recover it after illness. The items of the text are described in free translation and in summary. Some more explanations are added.

Animal Nutritional Physiological Phenomena↗

[Selenum carvifolia versus Peucedanum carfifolia. An historical look back on a botanical review and the first observation of the botanical work of H.J.N. Crantz].

The case history of Peucedanum carvifolia Vill. and Selinum carvifolia L. (Apiaceae/Umbelliferae) is on one hand the occasion for a first attempt of a historical review of H.J.N. Crantz's (1722-1797) publications on the Umbelliferae, and on the other hand the opportunity for a discussion of the taxonomical and nomenclatural peculiarities of both species.

Apiaceae↗