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At least 793 records · Page 44Linked to original sources
PubMed1988

Ultrasonography of rotator cuff tears: a review of 500 diagnostic studies.

Ultrasonography of the rotator cuff has been shown to be of value in diagnosing rotator cuff tears. This report summarizes our experience with our first 500 diagnostic examinations. All patients were examined in the hyperextended internal rotation view with commercially available high-resolution real-time ultrasound equipment. Patients were diagnosed as having a rotator cuff tear if a focal echogenic lesion or a defect within the rotator cuff was identified. This study confirmed the value of ultrasonography for the diagnosis of rotator cuff tears. Accuracy, sensitivity, and specificity all exceeded 90%, and correlated with surgical findings. This was better than arthrography in the same patient population. Ultrasound is an accurate noninvasive method of examining the rotator cuff for the presence of tears. We suggest that rotator cuff ultrasonography is the procedure of choice for the diagnosis of tears if adequate instrumentation is available.

Arthrography↗
PubMed1980

Palate morphogenesis: II. Contraction of cytoplasmic processes in ATP-induced palate rotation in glycerinated mouse heads.

It has been previously shown that non-muscle contractile system(s) exist in mouse palate mesenchyme underlying the palatal epithelium before shelf rotation. In order to obtain evidence that the non-muscle contractile system(s) function to elevate the palate, glycerinated heads have been incubated with ATP. It was shown that 5 mM ATP and a 30 min incubation at 25 degrees C stimulated palate rotation optimally. Elevation of the anterior end of the palate was nearly complete (PSI = 3.90, p less than 10(-6)). Although rotation of the posterior end was significant (p less than 0.02), movement was limited (PSI = 1.70). Light microscopy of the palate revealed that ATP caused a marked condensation of the cytoplasmic processes of the mesenchymal cells. The contraction of the processes of the mesenchymal cells induced by ATP increased roughly with increased palate shelf rotation and was greater at the peripheral than at the internal mesenchyme. Cytochalasin B pretreatment at 40 microM completely blocked the ATP-induced rotation at the anterior end. The effect of other nucleotides on palate rotation was tested. GTP caused a significant stimulation of anterior shelf rotation (p less than 0.005), which was less than ATP, while ADP and CTP were ineffective. Low temperature (6 degrees C) prevented the ATP-induced shelf rotation. These results suggest that the non-muscle contractile cells in the mesenchyme play a role in palate elevation and that contraction of the actomyosin containing microfilaments supplies the motive force.

Actomyosin↗
PubMed1994

Off-resonance rotating-frame spin-lattice relaxation of quadrupolar (spin-1) nuclei.

The validity of the formalism for the off-resonance rotating-frame spin-lattice relaxation experiment applicable to spin-1 quadrupolar nuclei was experimentally examined by considering two model systems, deuterated glycerol and deuterated benzene in castor oil, at different temperatures. When appropriately implemented, the deuterium off-resonance rotating-frame spin-lattice relaxation experiment provides spectral-intensity-ratio-dispersion data which agree remarkably well with those predicted by the theoretical formalism. The assumption of quadrupolar relaxation as the dominant relaxation mechanism, and rigid-rotor isotropic tumbling, permits the assessment of rotational diffusion behavior, i.e., the determination of a rotational correlation time, of a variety of molecular systems. With the inclusion of an additional relaxation measurement, T1 or T2, the 2H off-resonance rotating-frame spin-lattice relaxation experiment becomes a convenient method for the estimation of the 2H quadrupolar coupling constant, provided that a realistic reorientational motional model is assumed in the theoretical relaxation expressions used in the analysis. Because the motional window of the 2H off-resonance rotating-frame spin-lattice relaxation experiment includes intermediate molecular motion with correlation times as short as 1 or 2 ns, this experiment is appropriate for the investigation of the rotational diffusion behavior of deuterated molecules varying in size from moderately small to macromolecular. The 2H off-resonance rotating-frame spin-lattice relaxation experiment is applicable to in vitro and in vivo experimental situations.

Algorithms↗
PubMed1979

Pattern discrimination and visuomotor behavior following rotation of one or both eyes in kittens and in adult cats.

Sixteen cats, each of which had one or both eyes rotated at the time of natural eye opening (group K), were tested for visuomotor behavior and for learning and interocular transfer of two-choice visual discriminations. Their behavior was compared to that of two cats given monocular rotations in adulthood (group A) and to two normal controls (group N). These animals were all reared in the same colony. All cats, including those with monocular rotations up to 180 degrees and those with binocular rotations up to 80 degrees in each eye, showed good visuomotor behavior when using the rotated eye (i.e., with the normal eye covered). Both the group K and group A animals showed comparable visuomotor adaptation. All animals except those with monocular rotations of 180 degrees were able to learn several oriented pattern discriminations and showed considerable though incomplete interocular transfer of such information. The three animals with 180 degrees rotations were able to learn brightness, but not pattern discriminations. Seven further animals with large rotations were used for histological studies of the retina and primary visual pathways. Areas of reduced ganglion cell density were not observed in whole mounts of the retinae, nor were regions of reduced transport of 3H-proline from the retina to the lateral geniculate nuclei or superior colliculi detectable from autoradiographs.

Animals↗
PubMed2002

Remapping of place cell firing patterns after maze rotations.

When place cells are recorded from rats running on an elevated T-maze inside a curtained enclosure that contains distinct, experimenter selected stimuli, rotations of the maze plus stimuli cause equal rotations of firing fields. Here, we examined the effects of conflicting rotations of a T-maze relative to a laboratory frame that contained a large number of fixed stimuli in the environment and asked whether positional firing patterns stayed in register with the maze or the room cues or were modified in some more complex way. After maze rotations of 90 degrees, 180 degrees or 270 degrees, firing fields were stable in the laboratory frame and thus shifted to a different maze arm. In contrast, rotations of 45 degrees or -45 degrees resulted in dramatic changes of positional firing patterns regardless of their initial position on the maze. Crucially, even cells whose fields were initially on the central platform underwent major firing pattern alterations although the view of the environment from the platform was unchanged by such rotations. Finally, we found that altering the visual appearance by removing without rotation one or two maze arms did not alter firing fields on the remaining part of the maze. Thus, the "remappings" caused by 45 degrees rotations could result from the disturbed relationship between all arms and the room cues or from the changes in the possible paths the animal can take in the environment. Taken together, our results provide an example of combinatorial coding by the hippocampus, in which the place cell representation of the environment was seen to be modified as a unit and not piecewise according to locally available stimuli.

Animals↗
PubMed1999

Dorsal instrumentation for idiopathic adolescent thoracic scoliosis: rod rotation versus translation.

The radiographic and clinical outcomes and complications among two groups of adolescent patients treated for idiopathic thoracic scoliosis with dorsal instrumentation using a unified implantation system (Universal Spinal System) were compared retrospectively. A total of 69 patients were included in the study. In 30 patients an intraoperative correction of the scoliosis was performed by translation and segmental correction (translation group, Helsinki). In 39 patients the correction was achieved according to the Cotrel-Dubousset rod rotation maneuver (rod rotation group, Berlin). The goal of the present study is to investigate whether one of the operative procedures leads to a better correction of idiopathic adolescent thoracic scoliosis than the other. The mean follow-up interval was 30 months, with a minimum of 12 months. There were no significant preoperative differences in age (15+/-2 years in both groups), gender, or type of scoliosis (King types 2, 3, and 4). The preoperative radiographic measurements showed no significant differences between the two groups. In both patient groups, the thoracic primary curve, the lumbar secondary curve and the thoracic apical rotation were improved by the operation. Lumbar apical rotation and the sagittal profile were unchanged in both groups. The thoracic primary curve was corrected from 50 degrees +/-6 degrees to 24 degrees +/-7 degrees in the translation group and from 54 degrees +/-11 degrees to 220 degrees +/-11 degrees in the rod rotation group. The extent of the correction of the thoracic curve was significantly greater in the rod rotation group than in the translation group (59% vs. 52% correction). In contrast, the translation procedure seems to have a more beneficial effect on spinal balance than rod rotation. Neurological complications did not occur. In both patient groups an increase in the non-instrumented lumbar curve was noted, in two cases each. In three patients from the rod rotation group the instrumentation had to be removed due to a late infection with negative microbiological results.

Adolescent↗
PubMed1987

Electro-rotation of mouse oocytes: single-cell measurements of zona-intact and zona-free cells and of the isolated zona pellucida.

Passive electrical properties of oocytes and of zonae pellucidae, and the mechanical coupling between them, can be elucidated by means of rotating-field-induced rotation. In low-conductivity media (25-100 microS/cm) rotation of mouse oocytes (with or without their zonae) requires fields in the 1-100 kHz frequency range. However, an isolated zona shows weak rotation in the opposite direction to that of a cell, and in response to much higher field frequencies (approx. 1 MHz). In zona-intact mouse oocytes, the rotation of cell and zona are not rigidly coupled: thus rotation of the cell can still be induced when the zona is held stationary. However, rotation of freely suspended zona-intact cells is much slower than that of zona-free cells and requires an optimum field frequency that is approximately 1.5 kHz higher. These observations show that the electrical properties of the oocyte that are measured by rotation are altered by the presence of the zona pellucida, even though no such influence has been detected using micro-electrodes. The data are consistent with the zona acting as a porous shell with a conductivity of 40 microS/cm (preliminary estimate made at a single medium conductivity of 26 microS/cm). Measurements on cells from which the zonae had been removed gave values for the membrane capacity and resistivity of 1.2-1.3 microF/cm2 and 400 omega.cm2, respectively. These values may reflect the presence of plasmalemma microvilli. The results strongly suggest that the technique may be useful for studies of cell maturation and for in vitro fertilization, because the cells may be further cultured after measurement.

Animals↗
PubMed1996

Forces on biological cells due to applied alternating (AC) electric fields. II. Electro-rotation.

Measurements are presented of angular velocities of rotation of mammalian cells of K562 (human) and SP2 (mouse) in external alternating electric fields over a frequency range of 0.5 kHz to 12 MHz. Electro-rotation of the cells was observed for the case of "two cells in contact' using two parallel, cylindrical electrodes; only one cell was located on the electrode. A theoretical analysis is also presented which shows that the cell rotation arises from a torque produced by the interaction between the primary electric dipole moment induced in the spinning cell and the secondary electric fields, generated by the primary dipole induced in the adjacent cell. These secondary fields are out of phase with the applied electric field. The results show that (a) only the cell not located on the electrode rotates, (b) maximal electro-rotation occurs at two different excitation field frequency domains for the frequency range employed here, (c) the spin speed of the rotating cell at each frequency domain is much less than the excitation frequency, (d) the rotation direction of the cell depends on the angle (theta) between the external electric field and the line joining the centres of the two cells and (e) for a given angle theta, the rotation direction is the same for both excitation frequency domains. The experimental measurements allowed us to estimate the conductivities of the cytoplasms and membrane capacitances of the cells of K562 and SP2. The conductivities of the cytoplasms of the cells of K562 and SP2 were estimated to be 0.2 and 0.3 Sm-(1), respectively, whereas the membrane capacitances of these cells were found to be 2.7 +/- 0.8 and 9.8 +/- 0.6 mFm-(2), respectively.

Animals↗
PubMed1983

Differentiation of dopamine agonists using drug-induced rotation in rats with unilateral or bilateral 6-hydroxydopamine destruction of ascending dopamine pathways.

Eighteen compounds with dopamine agonist properties were examined in two rat rotational models. In the classical Ungerstedt model, a unilateral 6-hydroxydopamine (60HDA) lesion destroyed nigrostriatal and mesolimbic dopamine pathways on one side. Indirectly acting compounds, amphetamine, amantadine, methylphenidate and S1694, produced ipsiversive rotation, which was inhibited by pretreatment with alpha-methyl-p-tyrosine (AMPT). All other compounds produced contraversive rotation, but the rotation caused by CM 29-712, bromocriptine and ET 495 was reduced by AMPT. In animals with a bilateral 60HDA lesion removing both dopaminergic inputs to nucleus accumbens and the dopaminergic input into one striatum, indirectly acting drugs caused ipsiversive posturing prevented by AMPT, but little rotation. All other compounds produced contraversive rotation, but the effects of CM 29-712, bromocriptine and ET 495 were reduced by AMPT pretreatment. Inhibition of monooxygenase drug metabolising activity utilising SKF-525A inhibited contraversive turning induced by bromocriptine and ET 495 in the unilateral lesion model, but had no effect on rotation caused by apomorphine or CM 29-712. We conclude that, in addition to indirect pre-synaptically acting agonists and direct post-synaptic receptor agonists, there are a small group of compounds which produce rotation associated with direct receptor activation, which is inhibited by disruption of pre-synaptic dopamine function. The mechanism of action of this latter group is not understood, but cannot be attributed solely to active metabolite formation.

Animals↗
PubMed1983

Electroencephalographic studies of chlorpromazine methiodide and somatostatin-induced barrel rotation in rats.

It has been reported that intraventricular injection of chlorpromazine methiodide (CPZMI), a quaternary ammonium derivative of chlorpromazine, in rats induces abnormal, twisting postures which may serve as an experimental model of the human movement disorder dystonia. We have shown elsewhere that the behavior induced by intraventricular CPMZI is identical to what has been called "barrel rotation," first observed to follow intraventricular injection of somatostatin (SRIF), which consists of twisting about the long axis, with repetitive lateral rolling. The suitability of barrel rotation, induced by CPZMI or SRIF, as an experimental model for dystonia depends on its physiologic basis. Human dystonia is clinically not a convulsive phenomenon. SRIF-induced barrel rotation has been reported to be associated with epileptiform activity recorded by the electroencephalogram (EEG). The purpose of this study was to investigate EEG activity during CPZMI- and SRIF-induced rotation. We found that CPZMI barrel rotation was not associated with epileptiform activity in cortex, amygdala, or hippocampus, and contrary to prior reports, neither was SRIF rotation. Both CPZMI and SRIF injected in high doses could induce epileptiform activity, but this was associated with clonic motor phenomena and not barrel rotation. We conclude that electroencephalographic criteria do not exclude either CPZMI- or SRIF-induced rotation as models for movement disorders, but their validity as such requires further study.

Animals↗
PubMed1987

Effects of centrifugal rotation on analgesia and conditioned flavor aversions.

In the first experiment, 48 female Wistar rats were water deprived and given three conditioning days with saccharin-flavored water (1.5 g/liter) followed by 0, 5, 10 or 15 min of centrifugal rotation (150 rpm). Analgesia was measured by the tail flick test immediately after rotation. Over conditioning days, taste aversions developed. In general, taste aversion strength increased with duration of rotation. Analgesia also was in proportion to duration of rotation; however, over days tolerance developed in all rotated groups. In the second experiment 12 female Wistar rats were water deprived and given naloxone (20 mg/kg) or saline prior to 15 min of rotation. Rotation-induced analgesia was not affected by naloxone. It was concluded that somatic and gastrointestinal reactions to rotation are not served by a single mechanism.

Animals↗
PubMed1988

GABAergic mechanisms mediating rotational behavior in rats: differences between dorsal and ventral striatum.

Local injection of muscimol into the dorsal or ventral striatum of rats did not induce any rotational activity, but injection of picrotoxin into ventral striatal aspects induced an intense contralateral rotation. Amphetamine pretreatment resulted in an ipsilateral rotation after muscimol injection into dorsal striatum and in a complex rotational pattern after ventral injection, characterized by an initial short-lasting ipsilateral rotation followed by a long-lasting contralateral rotation. The contralateral rotation induced by picrotoxin was abolished by amphetamine pretreatment. These results indicate a functional heterogeneity between dorsal and ventral striatal mechanisms mediating rotational behavior, and a different pattern of dopamine-GABA interactions between dorsal and ventral striatum.

Animals↗
PubMed1996

Rotational laxity and stiffness of the radiocarpal joint.

OBJECTIVE: To investigate the constraint and potential mechanism of torque transmission across the wrist joint. DESIGN: In vitro experiment using human cadaveric specimens. BACKGROUND: Transmission of torque from the forearm to the hand requires rotational stability at the wrist. Better appriciation of the constraints would have applicability to several clinical problems where the stability is compromised. METHODS: Thirteen fresh-frozen cadaveric specimens were used in this experiment to investigate the rotational laxity and stiffness of the radiocarpal joint in unloaded and axially loaded (100 N) conditions, and three forearm orientations in a neutral, pronation (60 degrees ), or supination (60 degrees ) position. RESULTS: In pronation or supination, there was no difference between loaded and unloaded conditions in primary or total laxity at a maximum torque of 2.3 Nm. Unloaded specimens showed a mean total rotational laxity of 42.1 degrees. Supination or pronation of the forearm caused a decrease in laxity with respect to neutral forearm rotation (35 degrees and 41.6 degrees versus 49.6 degrees respectively). The primary rotational laxity accounted for half of the total laxity. With axial compression, total rotational laxity did not change, but primary laxity dropped to 50% of its unloaded value. The primary stiffness was very low -- approximately 11% of the secondary stiffness. CONCLUSION: The ligamentous structures and the joint articulation restricted excessive axial rotation of the wrist. However, a laxity of approximately 20 degrees was identified for normal wrists. RELEVANCE: This study demonstrated that the primary axial rotational laxity of the radiocarpal joint was approximately 20 degrees. In the mathematical model and implant design, muscular balance of the joint within such laxity should be considered.

Journal Article↗
PubMed1990

Gender, level of spatial ability, and lateralization of mental rotation.

The present study indicates that some of the inconsistencies in studies of the lateralization of mental rotation may be a consequence of uncontrolled individual differences in the general level of spatial ability. In order to investigate the relation between spatial ability and the lateralization of mental rotation, 48 subjects (24 males and 24 females) were divided into three groups based on their performance on a standardized test of spatial ability. They then performed a lateralized two-dimensional mental rotation task. The results showed the typical mental rotation function in that angle of rotation and reaction time were linearly related. A significant spatial ability by visual field interaction indicated that subjects with low spatial ability had a left field advantage, whereas subjects with medium spatial ability showed no field advantage and subjects with high spatial ability showed a right field advantage. Gender also interacted with visual field, with males showing a left visual superiority and females an insignificant right visual field advantage. A significant three-way interaction of gender, spatial ability, and angle of rotation reflected the fact that low spatial males were more profoundly affected by rotation than the other groups. The results suggest that at least some of the inconsistent findings in studies of lateralization of mental rotation may be accounted for by differences in the level of spatial ability.

Aptitude↗
PubMed2005

Recombinant RoTat 1.2 variable surface glycoprotein as antigen for diagnosis of Trypanosoma evansi in dromedary camels.

The transcript encoding a predominant Trypanosoma evansi variable surface glycoprotein RoTat 1.2 was cloned and expressed as a recombinant protein in Spodoptera frugiperda and Trichoplusia ni (insect) cells. Its potential as an antigen for specific detection of antibody in serum of dromedary camels affected by surra, was evaluated. In ELISA, the reactivity of the recombinant RoTat 1.2 VSG was similar to that of native RoTat 1.2 VSG. An indirect agglutination reagent was therefore prepared by coupling the recombinant RoTat 1.2 VSG onto latex particles. The performance of the latex agglutination test was evaluated on camel sera, and compared with the performance of CATT/T. evansi and LATEX/T. evansi tests, using the immune trypanolysis assay with T. evansi RoTat 1.2 as a reference test. The relative sensitivity and specificity of the latex coated with recombinant RoTat 1.2 VSG, using a 1:4 serum dilution, were respectively, 89.3 and 99.1%. No differences were observed between the performance of latex coated with recombinant RoTat 1.2 VSG and LATEX/T. evansi or CATT/T. evansi. Here, we describe the successful use of the recombinant RoTat 1.2 VSG for detection of specific antibodies induced by T. evansi infections.

Animals↗
PubMed2005

Evaluating the eye's rotational stability during standard photography: effect on determining the axial orientation of toric intraocular lenses.

PURPOSE: To evaluate the rotational stability of the eye during standard photography and determine its effect on the measurement of toric intraocular lens (IOL) orientation. SETTING: Department of Ophthalmology, University Erlangen-Nuremberg, Erlangen, Germany. METHODS: The rotational stability of the eye was evaluated using standard photographs taken with a telecentric fundus camera (Zeiss). Two sets of fundus images were taken at least 6 months apart in 400 eyes of 200 patients. The axial position of the eye was determined using 2 characteristic markers of the fundus. The angle between the 2 images (autorotation angle) was measured in each eye. RESULTS: The mean absolute autorotation was 2.3 degrees +/- 1.7 (SD) (range 0 to 11.5 degrees). Nine percent of eyes did not rotate. The rotation was less than 3 degrees in 55% of eyes and was 3 degrees or greater in 36% of eyes. Eyes of patients younger than 50 years rotated less than eyes in older patients (mean 2.2 +/- 1.5 degrees and 2.5 +/- 1.8 degrees, respectively) (P=.04). A visual acuity of 20/20 or better (P=.02) and a refractive cylinder of less than 1.75 diopters (P=.01) were correlated with smaller amounts of autorotation. Potential causes of artificial eye rotation induced by the photographic technique included camera adaptation (3-degree intrinsic error), slide mounting (<1 degree), slide projection (<0.5 degree), marking of characteristic fundus details (<1 degree), and head inclination. CONCLUSIONS: Cyclorotation of the eye during standard photography may lead to overestimation or underestimation of the presumed spontaneous rotation of an implanted toric IOL. Results show that 11.5 degrees of toric IOL rotation would lead to residual astigmatism that is 40% of the initial astigmatic power and 3 degrees, 10% of the initial power. Digital imaging may reduce the intrinsic errors of standard photography.

Adolescent↗
PubMed2005

Influence of axial rotation on chiropractic pelvic radiographic analysis.

OBJECTIVE: The aim of the study is to explore associations between axial (y-axis) rotation of the pelvis and pelvic radiographic measurements. STUDY DESIGN: Descriptive film statistics of an incrementally rotated pelvis and linear regression analysis were performed. METHODS: A phantom pelvic model was incrementally imaged (1 degrees increments) at 40-in source-to-image distance through 10 degrees of axial rotation. Chiropractic line drawing analysis was performed. The chiropractic examiner was blinded to the degree of rotation during the film analysis. Regression analysis was performed between axial rotation and pubic symphysis deviation, sacral width, and innominate and femur head heights. Each measurement corresponds to a chiropractic listing within the Gonstead system. RESULTS: Regression analysis revealed a strong association between the degree of axial rotation and each response variable: pubic symphysis deviation, sacral width, innominate height, and femur head height. The strongest relationship existed between y-axis rotation and pubic symphysis deviation. CONCLUSIONS: Chiropractic pelvic listings are strongly influenced by positioning of the subject. A few degrees of axial rotation may create apparent misalignments of several millimeters.

Chiropractic↗
PubMedDate not supplied

The effect of radiofrequency thermal capsulorrhaphy on glenohumeral translation, rotation, and volume.

The purpose of this study is to evaluate the effects of radiofrequency (RF) thermal capsulorrhaphy on the kinematic properties of the glenohumeral joint as determined by changes in resistance to multidirectional translational forces, alteration in the range of internal and external rotation, and changes in glenohumeral joint volume. Nonablative RF thermal energy was used to contract the glenohumeral joint capsule in 6 cadaveric shoulders. Measurements of translation were made after application of a 30-N load in anterior, posterior, and inferior directions. The maximum arc of internal and external rotation after application of a 1-N-m moment was also determined for vented specimens before and after thermal capsulorrhaphy. The percent reduction in glenohumeral capsular volume was measured by use of a saline solution injection-aspiration technique. Capsular shrinkage resulted in reductions in anterior, posterior, and inferior translation. The largest percent reductions in anterior translation were seen in external rotation at 45 degrees (48%, P <.05) and 90 degrees (41%, P <.05) abduction. For inferior translation, the largest percent reductions were seen in internal rotation at 45 degrees (40%, P <.05) and 90 degrees (45%, P <.05) abduction. Reductions in posterior translation were noted in internal rotation at 45 degrees (27%, P <.05) and 90 degrees (26%, P <.05) abduction. Other changes in translation were observed but were not statistically significant. The maximum arc of humeral rotation was reduced by a mean of 14 degrees at 45 degrees abduction and 9 degrees at 90 degrees abduction. The mean percent reduction in capsular volume for all shoulders was 37% (range, 8%-50%). This could not be correlated with percent reductions in translation and rotation. This study demonstrated the significant effect of RF thermal capsulorrhaphy in reducing glenohumeral multidirectional translation and volume with only a small loss of rotation in cadaveric shoulders.

Aged↗