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At least 793 records · Page 44Linked to original sources
PubMed1988

[Evaluation on the capability of indole pyruvic acid production by members of Proteeae on different brands of sulfide-indole-motility].

Members of Proteeae were vertically inoculated into different brands of sulfide-indole-motility (SIM) medium for evaluating the capability of IPA production. A total of 328 clinical strains of tribe Proteeae was used as tested organisms, including 186 Proteus mirabilis strains, 62 Morganella morganii strains, 31 Proteus vulgaris strains, 27 Providencia rettgeri strains, 14 Providencia stuartii and 8 Providencia alcalifaciens strains. Seven different brands of SIM medium used in this study are Kyokuto and Eiken from Japan; Gibco, Difco, Scott and BBL from U.S.A.; as well as Oxoid from England. The results indicate that SIM medium of kyokuto is the most suitable medium for Proteus spp. and Providencia spp. to produce IPA (positive rates are 95.4% and 93.0%, respectively) but not for Morganella morganii (16.1%). Eiken, Gibco and BBL SIM medium are only good for Providencia spp. to produce IPA (positive rates are 95.5%, 85.1% and 87.8%, respectively). Take account the tribe Proteeae the best SIM medium to detect IPA production was from Kyokuto (80%) and the worst was Oxoid (0.3%). Since SIM medium can constantly detect the production of H2S, the relationship between H2S and IPA was analyzed. We found that there is no obvious interaction between these two characters. Production of phenylalanine deaminase (PD) is one of the important characters for tribe Proteeae. We compared PD and IPA positive rates of each members and found that the ability to produce PD and IPA is almost identical for each member in tribe Proteeae except Morganella morganii.(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acid Oxidoreductases↗
PubMed1999

Rapid on-line microdialysis hyphenated technique for the dynamic monitoring of extracellular pyruvate, lactic acid and ascorbic acid during cerebral ischemia.

Rapid on-line microdialysis coupled with liquid chromatography was developed for the continuous monitoring of brain neurochemicals during cerebral ischemia. Isocratic separation of these analytes was achieved within 3 min, hence, over 80 analyses could be performed in a 4-h experiment. The dead volume of the microdialysis system was estimated to be less than 10 microl. The detection limits of the present assay, at a signal-to-noise ratio of five, were 2.0, 0.2 and 0.5 microM, for lactic acid, pyruvate and ascorbic acid, respectively. To validate this assay, a transient ischemia was produced by occlusion of two common carotid arteries for 10 min in an anesthetized gerbil. A microdialysis probe was inserted into the striatum of the gerbil to simultaneously monitor pyruvate, lactic acid and ascorbic acid during cerebral ischemia. Significant and dynamic changes in these analytes could be resolved in 3-min intervals. This rapid assay can be used as a tool to study dynamic changes in neurochemicals of the brain, such as during cerebral ischemia.

Animals↗
PubMed1975

[Fixed acids in protracted parturition].

The levels of lactic acid, pyruvic acid, and L/P index were studied in 15 cows during protracted parturition. All the indices under study respond to the stress to which the organism is exposed as a result of continuing parturient activity:the indices increase in their values, and the peak values occur when the foetus is delivered. The levels of lactic acid and pyruvic acid drop immediately after parturition and the L/P index values decrease from the first hour post partum.

Anaerobiosis↗
PubMed1975

[Fixed acids in physiological parturition].

The levels of lactic acid, pyruvic acid, and L/P index in blood were studied. During physiological parturition in 25 cows it was found that the levels of lactic acid and pyruvic acid showed an increase during parturition, and this increase reached a maximum at the expulsion stage. When foetus is delivered and parturient activity decreases, the levels of the two metabolites decline gradually. The increased values of the L/P index showing the saturation of tissues with oxygen indicate some degree of oxygen starvation during parturition which does not stop when foetus is out but persists some time after parturition.

Anaerobiosis↗