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PubMed2006

Effects of velocity gradient and mixing time on particle growth in a rapid mixing tank.

Effects of velocity gradient (G value) and mixing time (t) on the coagulation of particles were investigated by monitoring particle counts in a rapid mixing process. Total particle counts after mixing of 30 s at G value of 200 s(-1) were similar to the summation of clay particles and alum precipitates at alum dose of 30 mg L(-1). Although small particles (microflocs less than 5 microm) were formed within the mixing time of 30 s, macroflocs larger than 8 microm did not increase significantly until the mixing time of 60 s. However, macroflocs larger than 8 microm started to increase after mixing of 75 s and they reached the maximum counts at 150 s. On the other hand, macroflocs larger than 8 microm decreased after mixing time of 180 s due in breaks of the macroflocs, which resulted in resuspension of small particles. The rapid mixing conditions for the maximum growth of macroflocs were the G value of 200 s(-1) and the mixing time of 150 s, which confirmed the best performance of turbidity removal in jar tests. Growth and break of particles and flocs in a rapid mixing step would influence significantly on the sequential flocculation and sedimentation processes for turbidity removal.

Alum Compounds↗

PubMed2000

Flocculation of Saccharomyces cerevisiae is induced by transformation with the GAP1 gene from Kluyveromyces marxianus.

A non-flocculent strain of Saccharomyces cerevisiae was transformed with the GAP1 gene which encodes p37, a GAPDH-like protein present in the cell wall of Kluyveromyces marxianus flocculent cells. The transformed cells were characterized with respect to flocculation behaviour, morphology, growth, cell wall integrity and GAPDH activity. A flocculent phenotype was acquired by the transformed cells, showing a behaviour in respect to flocculation/deflocculation very similar to that of K. marxianus. The presence of p37 in the cell wall was assessed by immunoprecipitation of biotinylated cell wall proteins and an accumulation of p37 was evident in the cell wall of transformed cells. This result was confirmed by studies using a chimeric protein resulting from fusing the p37 with a yeast-enhanced green fluorescent protein, yEGFP. The recombinant protein was localized mainly in the cell wall of the transformed strain, although the presence of p37 in the cytosol was indicated by an increase in GAPDH activity. Calcofluor white sensitivity tests indicated that the cell wall structure is affected by the accumulation of p37. These results provided further evidence of p37 function regarding flocculation and that although lacking a N-terminal signal peptide p37 is targeted to the cell wall.

Biomass↗

PubMed2006

Extracellular polymers in partly ozonated return activated sludge: impact on flocculation and dewaterability.

The purpose of this study was to evaluate the influence of partial ozonation of return activated sludge on settling properties and dewaterability of sludge. Sequencing batch reactors with two sets of aerobic and alternating anoxic/aerobic conditions were used. In each set, one reactor served as a control and the other was subject to the ozone treatment (doses in the range of 0.016-0.080 mg O3/mg TSS of initial excess sludge). The level of total suspended solids (TSS) in each reactor was controlled at 1,800 mg/l. To evaluate settleability and dewaterability, settling kinetic studies, sludge volume index (SVI) and capillary suction time test (CST) were used. For extraction and quantifying sludge biopolymers, thermal-ethanolic extraction was employed. The ratio of bound-to-total extracellular polymer substances (EPS) was higher for the strictly aerobic reactor than for the alternating anoxic/aerobic one, indicating the stronger structure of the aerobic flocs. After ozone treatment, the fraction of bound EPS was released and solubilized, increasing soluble EPS. Increased apparent food-to-microorganism (F/M) ratio favoured production of EPS in ozonated reactors, enhancing flocculation, which had potential to improve settling. Dewaterability, measured by CST test, was better in alternating anoxic/aerobic reactors than in aerobic ones, indicating that incorporation of an anoxic zone for biological nutrient removal leads to improvement in sludge dewatering. The negative impact of ozonation on dewaterability was minimal in terms of the long-term operation.

Aerobiosis↗

PubMed2003

Electrokinetic characteristic and coagulation behavior flocculant polyaluminum silicate chloride (PASiC).

The electrokinetic characteristics and coagulation behaviors of polyaluminum silicate chloride (PASiC) and polyaluminum chloride (PAC) were studied and compared by streaming current (SC) measurement and jar test method. The experimental results showed that the interaction between polysilicic acid characterized negative charge and hydrolyzed aluminum species result in a decrease of the charge-neutralizing ability of PASiC, compared to PAC. The decrease has a close relationship with the basicity (B) and Al/Si molar ratio in PASiC. The less the B value and the Al/Si molar ratio, the lower the charge-neutralizing ability of PASiC is. In contrast, the preparation technique for PASiC affects the charge - neutralization of PASiC to a smaller extent. In addition, compared with PAC, PASiC may enhance aggregating efficiency and give better coagulating effects.

Aluminum Hydroxide↗

PubMedDate not supplied

Flocculation: an alternative process to ion-exchange chromatography: (A scale-up study using recombinant human superoxide dismutase as model protein).

Flocculation using pellicular charged flocculants was investigated as an alternative process to conventional chromatography in purification of recombinant proteins using human recombinant superoxide dismutase expressed in E. coli as a model system. The removal of pyrogens, proteins, debris and the yield were determined. At laboratory scale, the starting conditions were optimized to yield a stable solution and the flocculation process fitted into a purification scheme. 100 L fermentation broth was the initial volume at pilot scale. The process parameters determined at the laboratory scale were tested and the results were compared to the pilot scale. The method was also compared to an ion-exchange chromatography.

Chromatography, Ion Exchange↗

PubMed2005

Acute toxicity removal in textile finishing wastewater by Fenton's oxidation, ozone and coagulation-flocculation processes.

This study evaluates the effectiveness of Fenton's oxidation (FO) process and ozone (O3) oxidation compared with a coagulation-flocculation (CF) process to remove effluent toxicity as well as colour and COD from a textile industry wastewater. Daphnia magna was used to test acute toxicity in raw and pre-treated wastewater. The operational parameters for each process were determined on the basis of complete toxicity removal. The FO process removed COD at a higher rate (59%) than O3 (33%) while colour removal was similar (89% and 91%, respectively). The CF process removed both COD and colour at rates similar to the FO process. A colour range of 150-250 platin-cobalt (Pt-Co) unit was assessed for toxicity.

Flocculation↗

PubMed1994

Continuous ethanol production by flocculating yeast in the fluidized bed bioreactor.

Continuous fermentation by a highly flocculant strain of the yeast Saccharomyces cerevisiae was carried out in a tower fluidized-bed bioreactor. The synthetic and molasses media with a total sugar concentration of 17% (w/v) were used for fermentation. Different dilution rates were tested. Stable cell densities of 50 kg m-3 (dry weight) were maintained for all dilution rates. The ethanol productivity was increasing linearly with dilution rates up to 15-20 kg m-3 h-1. Aeration of the culture stabilized flocculating activity and viability of yeast and also permitted long-term operation of the bioreactor.

Culture Media↗

PubMed2004

Integrated Fenton's reagent-coagulation/flocculation process for the treatment of cork processing wastewaters.

In the present work two methods were tested for the treatment of cork processing wastewaters. In a first phase, a coagulation/flocculation method was applied using FeCl3 as flocculating agent and Ca(OH)2 as base-precipitant. One cycle of the treatment (1000 ppm of FeCl3) reduced chemical oxygen demand (COD), total polyphenols and aromatic compounds of the effluent by 45, 71 and 58%, respectively. Two cycles of treatment (2x 1000 ppm of FeCl3) increase these reductions until values of 67, 92 and 85%, respectively. In a second phase, an integrated Fenton-coagulation/flocculation process was applied (this second method only requires the addition of H2O2). One cycle of the integrated process (1000 ppm of FeCl3 and 0.5 mol/l of H2O2) reduced the COD, total polyphenols and aromatic compounds of the effluent by 74, 99 and 98%, respectively, which supposes a considerable improvement compared with the first method.

Coagulants↗

PubMed1989

Large community outbreak of cryptosporidiosis due to contamination of a filtered public water supply.

Between January 12 and February 7, 1987, an outbreak of gastroenteritis affected an estimated 13,000 people in a county of 64,900 residents in western Georgia. Cryptosporidium oocysts were identified in the stools of 58 of 147 patients with gastroenteritis (39 percent) tested during the outbreak. Studies for bacterial, viral, and other parasitic pathogens failed to implicate any other agent. In a random telephone survey, 299 of 489 household members exposed to the public water supply (61 percent) reported gastrointestinal illness, as compared with 64 of 322 (20 percent) who were not exposed (relative risk, 3.1; 95 percent confidence interval, 2.4 to 3.9). The prevalence of IgG to cryptosporidium was significantly higher among exposed respondents to the survey who had become ill than among nonresident controls. Cryptosporidium oocysts were identified in samples of treated public water with use of a monoclonal-antibody test. Although the sand-filtered and chlorinated water system met all regulatory-agency quality standards, sub-optimal flocculation and filtration probably allowed the parasite to pass into the drinking-water supply. Low-level cryptosporidium infection in cattle in the watershed and a sewage overflow were considered as possible contributors to the contamination of the surface-water supply. We conclude that current standards for the treatment of public water supplies may not prevent the contamination of drinking water by cryptosporidium, with consequent outbreaks of cryptosporidiosis.

Cryptosporidiosis↗

PubMed1998

Breeding of flocculent industrial alcohol yeast strains by self-cloning of the flocculation gene FLO1 and repeated-batch fermentation by transformants.

A nonflocculent industrial polyploid yeast strain, Saccharomyces cerevisiae 396-9-6V, was converted to a flocculent one by introducing a functional FLO1 gene at the URA3 locus. The flocculent strain FSC27 obtained was a so-called self-cloned strain, having no bacterial DNA. FSC27 cells could be easily recovered for reuse from fermentation mash without any physical energy. The strain produced a concentration of alcohol as high as 396-9-6V, although the fermentation rate of FSC27 was slightly lower than that of 396-9-6V. When uracil was added to the medium or when URA3 was reintroduced into FSC27 (named FSCU-L18), the fermentation rate and the growth rate increased, and the ethanol concentration produced was higher than that produced by the parent strain. The stable flocculation and high ethanol productivity were observed by using FSCU-L18 during 10 cycles of repeated-batch fermentation test.

Journal Article↗

PubMed1955